Standard name | |
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Human Ortholog | |
Description | Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Bud | 0 | 1 | 1 | 1 | 0 | 5 | 2 | – | 1 | 2 | 1 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 10 | 5 | 3 | 8 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 3 | 2 | 1 | – | 2 | 3 | 4 | 1 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 77 | 459 | 395 | 284 | 308 | 282 | 410 | – | 256 | 215 | 172 | 207 | 298 | 443 | 412 | 158 | 214 | 195 | 76 | 419 | 383 | 244 | 321 | 315 |
Endoplasmic Reticulum | 1 | 4 | 1 | 0 | 1 | 0 | 0 | – | 1 | 3 | 0 | 1 | 1 | 0 | 1 | 10 | 5 | 9 | 0 | 0 | 3 | 2 | 1 | 1 |
Endosome | 0 | 0 | 2 | 0 | 5 | 0 | 4 | – | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 3 | 0 | 2 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 4 | 0 | 0 | 2 | 1 |
Mitochondria | 1 | 8 | 1 | 9 | 6 | 67 | 53 | – | 103 | 82 | 99 | 127 | 0 | 0 | 2 | 2 | 4 | 1 | 0 | 5 | 4 | 2 | 1 | 1 |
Nucleus | 0 | 9 | 1 | 2 | 6 | 6 | 12 | – | 12 | 19 | 13 | 27 | 0 | 2 | 0 | 7 | 3 | 6 | 0 | 19 | 5 | 2 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 2 | 3 | 3 |
Vac/Vac Membrane | 1 | 16 | 39 | 53 | 48 | 25 | 162 | – | 45 | 45 | 56 | 42 | 6 | 8 | 4 | 29 | 39 | 38 | 0 | 9 | 2 | 1 | 0 | 4 |
Unique Cell Count | 80 | 477 | 412 | 323 | 348 | 331 | 568 | 352 | 320 | 288 | 353 | 304 | 453 | 419 | 170 | 237 | 218 | 84 | 489 | 425 | 266 | 355 | 351 | |
Labelled Cell Count | 80 | 498 | 440 | 350 | 377 | 388 | 646 | 420 | 370 | 348 | 427 | 307 | 456 | 424 | 207 | 270 | 250 | 84 | 489 | 425 | 266 | 355 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.8 | 6.6 | 6.4 | 6.9 | 4.8 | 6.1 | – | 5.4 | 5.6 | 5.7 | 5.3 | 7.0 | 7.3 | 7.0 | 10.4 | 11.0 | 10.6 | 5.6 | 6.0 | 6.2 |
Std Deviation (1e-4) | 1.2 | 1.1 | 1.6 | 1.4 | 1.5 | 1.2 | 1.4 | – | 1.2 | 1.2 | 2.5 | 1.2 | 1.7 | 1.7 | 1.5 | 2.3 | 2.2 | 2.3 | 1.4 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.07 | -0.45 | -0.12 | – | -0.29 | -0.24 | -0.21 | -0.32 | 0.08 | 0.16 | 0.09 | 0.66 | 0.74 | 0.68 | -0.23 | -0.13 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.0 | -3.8 | -5.1 | -9.6 | – | -9.0 | -10.3 | -12.0 | -12.5 | 1.6 | 1.6 | 2.1 | -1.5 | -2.8 | -3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 6.2 | – | 11.7 | 10.7 | 12.7 | 13.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.5 | – | 3.4 | 4.7 | 4.0 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.8 | 1.9 | -0.9 | 7.3 | – | 1.5 | 1.9 | 3.8 | 1.1 | -4.1 | -5.0 | -5.5 | 2.6 | 2.6 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0233 | 0.0009 | 0.0043 | 0.0372 | 0.0037 | 0.0107 | 0.0196 | 0.0005 | 0.0156 | 0.0391 | 0.0192 | 0.0017 | – | – | – | – | – | – |
Bud | 0.0005 | 0.0013 | 0.0003 | 0.0018 | 0.001 | 0.0004 | 0.0009 | 0.0002 | 0.002 | 0.0006 | 0.0028 | 0.0003 | – | – | – | – | – | – |
Bud Neck | 0.0064 | 0.0004 | 0.0007 | 0.0009 | 0.0011 | 0.0137 | 0.0009 | 0.0039 | 0.005 | 0.0015 | 0.0158 | 0.0012 | – | – | – | – | – | – |
Bud Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0023 | 0.0011 | 0.0006 | 0.0007 | 0 | 0.0013 | 0.0008 | 0.0031 | 0.0001 | – | – | – | – | – | – |
Bud Site | 0.0062 | 0.0021 | 0.001 | 0.0022 | 0.0009 | 0.0003 | 0.0026 | 0.0021 | 0.0041 | 0.0019 | 0.0506 | 0.0002 | – | – | – | – | – | – |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0005 | 0 | – | – | – | – | – | – |
Cytoplasm | 0.6402 | 0.8453 | 0.8597 | 0.7045 | 0.779 | 0.8295 | 0.7618 | 0.9073 | 0.7825 | 0.8226 | 0.8037 | 0.87 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0319 | 0.0095 | 0.0069 | 0.0202 | 0.0177 | 0.0169 | 0.0136 | 0.0067 | 0.0126 | 0.017 | 0.0084 | 0.0105 | – | – | – | – | – | – |
Eisosomes | 0.0003 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0078 | 0.0053 | 0.0045 | 0.0031 | 0.0034 | 0.0046 | 0.0049 | 0.0019 | 0.0031 | 0.0086 | 0.0061 | 0.0045 | – | – | – | – | – | – |
Endosome | 0.034 | 0.0049 | 0.0066 | 0.0191 | 0.018 | 0.0123 | 0.0132 | 0.0014 | 0.0126 | 0.0102 | 0.0125 | 0.0052 | – | – | – | – | – | – |
Golgi | 0.015 | 0.0008 | 0.0023 | 0.0075 | 0.0042 | 0.0015 | 0.0048 | 0.0003 | 0.0053 | 0.0059 | 0.0018 | 0.001 | – | – | – | – | – | – |
Lipid Particles | 0.0255 | 0.0011 | 0.0013 | 0.0211 | 0.0011 | 0.0027 | 0.0051 | 0.0001 | 0.0031 | 0.0044 | 0.0008 | 0.0002 | – | – | – | – | – | – |
Mitochondria | 0.0157 | 0.0006 | 0.0019 | 0.0044 | 0.0116 | 0.0011 | 0.0041 | 0.0002 | 0.0108 | 0.0022 | 0.0013 | 0.0015 | – | – | – | – | – | – |
None | 0.0861 | 0.0616 | 0.0448 | 0.0883 | 0.0607 | 0.0247 | 0.0673 | 0.0268 | 0.0356 | 0.0301 | 0.0176 | 0.0169 | – | – | – | – | – | – |
Nuclear Periphery | 0.0146 | 0.0061 | 0.0055 | 0.0053 | 0.0154 | 0.0089 | 0.0145 | 0.0027 | 0.0059 | 0.0097 | 0.0203 | 0.0088 | – | – | – | – | – | – |
Nucleolus | 0.0071 | 0.0004 | 0.0001 | 0.0012 | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0015 | 0.0002 | 0.0005 | 0.0005 | – | – | – | – | – | – |
Nucleus | 0.051 | 0.0425 | 0.052 | 0.0327 | 0.0404 | 0.0577 | 0.0688 | 0.043 | 0.086 | 0.0307 | 0.0253 | 0.0646 | – | – | – | – | – | – |
Peroxisomes | 0.0111 | 0.0089 | 0.0035 | 0.0262 | 0.0168 | 0.0016 | 0.0072 | 0.0007 | 0.0048 | 0.0079 | 0.0011 | 0.0024 | – | – | – | – | – | – |
Punctate Nuclear | 0.0183 | 0.0064 | 0.0028 | 0.02 | 0.0197 | 0.01 | 0.0063 | 0.0016 | 0.0061 | 0.0046 | 0.0059 | 0.0095 | – | – | – | – | – | – |
Vacuole | 0.0029 | 0.0013 | 0.0011 | 0.0014 | 0.0027 | 0.002 | 0.0021 | 0.0007 | 0.0011 | 0.001 | 0.002 | 0.0007 | – | – | – | – | – | – |
Vacuole Periphery | 0.0013 | 0.0003 | 0.0003 | 0.0005 | 0.0007 | 0.0005 | 0.0009 | 0.0001 | 0.0005 | 0.0007 | 0.0008 | 0.0003 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.9413 | 63.056 | 49.0184 | 47.3023 | 45.7273 | 58.7485 | 57.3044 | 53.2764 | 59.8981 | 52.6802 |
Translational Efficiency | 1.6018 | 1.1525 | 1.5324 | 1.4773 | 1.8815 | 1.744 | 1.3133 | 1.4682 | 1.4928 | 1.5431 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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