Standard name
Human Ortholog
Description Mitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0.09 0.15 0.12 0.05 0.05 0 0.08 0.07 0.07
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.12 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.17 0.31 0.08 0.2 0 0 0 0 – 0 0 0 0.48 0.5 0.64 0.77 0.81 0.86 0.08 0.17 0.12 0.19 0.21 0.17
Mitochondria 0.7 0.76 0.92 0.83 0.84 0.88 0.87 0.86 – 0.89 0.92 0.91 0.66 0.58 0.4 0.13 0.12 0.09 0.72 0.65 0.73 0.58 0.52 0.58
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.06 0.05 – 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.07 0.14 0.11 0.17 0.21 0.2 0.2 – 0.19 0.18 0.18 0.09 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.17 0 0.1 0.08 0 0 0 – 0 0 0 0.18 0.18 0.26 0.13 0.11 0.14 0 0.05 0 0.06 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 16 4 4 1 1 2 3 – 2 3 1 22 6 6 22 32 21 3 21 11 19 27 22
Bud 0 3 2 1 2 8 5 6 – 9 4 11 2 0 2 3 4 3 0 4 1 4 6 6
Bud Neck 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 5 2 1 – 7 4 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 16 3 1 1 1 0 1 – 4 0 0 8 2 6 14 11 8 0 1 0 0 0 0
Cytoplasm 7 6 1 5 6 2 4 3 – 0 3 1 1 4 8 22 12 13 2 2 0 0 0 0
Endoplasmic Reticulum 0 2 2 2 1 1 4 3 – 3 4 3 5 5 5 6 8 7 0 1 0 0 2 1
Endosome 0 0 1 1 2 1 2 0 – 2 0 0 0 0 1 2 2 0 0 8 4 6 9 4
Golgi 10 119 22 29 11 1 5 3 – 10 2 1 183 140 191 194 175 145 5 75 33 44 87 58
Mitochondria 42 293 251 120 246 229 361 358 – 461 304 428 253 164 120 32 26 15 49 287 211 137 213 194
Nucleus 0 2 0 1 0 5 4 4 – 1 1 2 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 3 5 2 7 8 26 20 – 31 17 20 4 2 1 0 1 0 1 2 1 1 2 2
Nucleolus 7 28 39 16 50 54 81 84 – 97 61 86 34 13 8 11 5 3 0 5 2 1 3 9
Peroxisomes 0 0 4 1 1 0 1 3 – 8 2 2 1 3 0 0 0 0 0 4 10 4 16 4
SpindlePole 0 1 0 0 1 0 2 1 – 5 0 3 0 0 0 1 1 0 0 1 1 0 2 0
Vac/Vac Membrane 6 66 10 14 24 9 15 14 – 10 11 11 68 50 77 32 24 24 2 23 6 13 33 23
Unique Cell Count 60 388 272 145 292 260 413 414 516 330 471 382 281 298 251 215 169 69 445 288 237 412 334
Labelled Cell Count 80 555 344 197 353 325 514 504 651 416 573 581 389 426 339 301 239 69 445 288 237 412 334


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.8 32.3 23.8 25.0 20.1 16.1 15.6 16.1 – 16.6 17.4 17.4 34.6 30.0 29.0 28.0 29.6 29.9 36.6 35.7 34.4
Std Deviation (1e-4) 8.0 8.8 6.9 6.1 5.4 5.4 5.5 4.9 – 6.2 6.9 6.3 9.6 8.2 8.4 8.3 8.9 8.4 12.5 12.1 12.5
Intensity Change (Log2) – – – 0.07 -0.24 -0.56 -0.61 -0.56 – -0.52 -0.45 -0.45 0.54 0.33 0.28 0.24 0.32 0.33 0.62 0.59 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 2.8 0 0 3.8 5.6 4.8
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 2.9 2.6 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 4.7 3.5 4.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 3.5 -2.2 -4.4 -4.5 -5.0 – -4.2 -4.7 -6.0 10.8 10.8 13.8 16.1 16.4 16.4
Mitochondria -3.0 -2.9 -1.6 -2.0 -2.4 – -1.3 -0.1 -0.7 -7.8 -9.2 -13.0 -18.2 -17.7 -17.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.5 0.9 2.7 2.0 – 2.7 2.2 1.8 0 0 0 0 0 0
Nucleolus -0.9 0.9 2.0 1.8 2.0 – 1.6 1.4 1.4 -2.2 -3.9 -5.1 -3.9 -4.6 -4.4
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 2.5 2.3 -0.1 0 -0.2 – -1.5 -0.2 -1.1 5.5 5.3 7.3 3.8 3.2 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.231 30.5583 28.5854 27.3598 27.4068 29.2251 39.6716 40.1852 39.6179 40.7681 33.4201 38.4478 38.2595 34.4172 36.3471 34.3147 37.0386 35.1159
Actin 0.0245 0.0342 0.0356 0.0327 0.0024 0.0154 0.0212 0.0273 0.0258 0.0068 0.02 0.0086 0.0064 0.0287 0.0216 0.0088 0.0073 0.0087
Bud 0.0007 0.0023 0.0017 0.0056 0.0007 0.0004 0.0004 0.0018 0.0005 0.0002 0.0004 0.0001 0.0004 0.0034 0.0015 0.0003 0.0007 0.0004
Bud Neck 0.0005 0.0009 0.001 0.0021 0.0003 0.0005 0.0003 0.0005 0.0003 0.0002 0.0001 0.0001 0.0003 0.0008 0.0009 0.0005 0.0004 0.0005
Bud Periphery 0.0013 0.0035 0.0026 0.0067 0.0013 0.0012 0.0006 0.0031 0.0008 0.0003 0.0008 0.0001 0.0008 0.0046 0.0025 0.001 0.001 0.0013
Bud Site 0.0017 0.0082 0.005 0.0075 0.0006 0.0005 0.0016 0.0044 0.0013 0.0002 0.0003 0.0002 0.0007 0.0113 0.0071 0.0003 0.0012 0.0003
Cell Periphery 0.0013 0.0064 0.0047 0.0029 0.0016 0.0008 0.0005 0.0033 0.0012 0.0003 0.0008 0.0002 0.0016 0.0039 0.0037 0.0015 0.0011 0.0016
Cytoplasm 0.0014 0.0009 0.001 0.0008 0.0025 0.0004 0.0043 0.0004 0.0004 0.0001 0.0003 0 0.0005 0.0009 0.0004 0.0002 0 0.0002
Cytoplasmic Foci 0.0133 0.0082 0.0146 0.0074 0.004 0.0028 0.0086 0.0067 0.003 0.0007 0.0034 0.0007 0.0052 0.0076 0.0044 0.0013 0.001 0.0019
Eisosomes 0.0017 0.0027 0.002 0.0015 0.0011 0.0013 0.0012 0.0018 0.0011 0.0008 0.0006 0.0006 0.0013 0.0033 0.0028 0.0051 0.0015 0.001
Endoplasmic Reticulum 0.0038 0.0083 0.0011 0.0102 0.0006 0.0129 0.0009 0.0008 0.0022 0.0001 0.0007 0.0005 0.0006 0.0084 0.001 0.0029 0.0011 0.0029
Endosome 0.0311 0.0306 0.025 0.0246 0.0064 0.0239 0.0072 0.0129 0.0105 0.0026 0.0121 0.0069 0.0168 0.031 0.0141 0.0123 0.0044 0.0209
Golgi 0.0402 0.0877 0.076 0.1103 0.0394 0.0863 0.0134 0.0547 0.0263 0.0166 0.0581 0.0466 0.0502 0.0752 0.0628 0.0466 0.028 0.081
Lipid Particles 0.0224 0.0171 0.0198 0.0044 0.0274 0.0109 0.0071 0.006 0.0039 0.0006 0.0085 0.002 0.0249 0.0238 0.0173 0.0157 0.034 0.0143
Mitochondria 0.7853 0.7076 0.743 0.7229 0.8493 0.8018 0.8981 0.8348 0.8999 0.9639 0.8674 0.913 0.8305 0.7193 0.8084 0.8792 0.8822 0.8033
None 0.0059 0.0001 0.0005 0.0002 0.0009 0.0001 0.0056 0.0001 0.0001 0 0.0002 0 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0042 0.0016 0.002 0.0018 0.0013 0.0019 0.0013 0.0004 0.0009 0 0.0002 0.0003 0.0005 0.0014 0.0005 0.0012 0.0006 0.004
Nucleolus 0.0112 0.0024 0.0065 0.0014 0.0111 0.0005 0.0042 0.0012 0.0021 0.0004 0.0007 0.0001 0.0093 0.0069 0.0058 0.0002 0.0009 0.0005
Nucleus 0.0015 0.0009 0.001 0.001 0.0112 0.0002 0.0007 0.0007 0.0005 0.0001 0.0003 0.0001 0.0009 0.0011 0.0014 0.0002 0.0002 0.0005
Peroxisomes 0.006 0.0046 0.0097 0.0037 0.0043 0.0021 0.0023 0.0033 0.0031 0.0012 0.002 0.0014 0.0019 0.0056 0.003 0.0006 0.0021 0.0011
Punctate Nuclear 0.0012 0.0001 0.0018 0.0002 0.0016 0.0002 0.0023 0 0 0 0 0 0.0002 0.0001 0.0001 0 0 0
Vacuole 0.0131 0.0305 0.0131 0.0233 0.0102 0.0047 0.0045 0.0118 0.0068 0.001 0.0069 0.002 0.0092 0.0201 0.0122 0.0037 0.0033 0.0111
Vacuole Periphery 0.0278 0.0413 0.0325 0.0284 0.0218 0.0313 0.0138 0.0239 0.0094 0.0039 0.0161 0.0164 0.0372 0.0426 0.0286 0.0185 0.0289 0.0445

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 215.1403 189.6877 194.7131 183.1173 164.3064 212.0757 197.9198 167.2029 213.5978 173.9819
Translational Efficiency 1.3642 1.3057 1.3041 1.7429 1.6038 1.5542 1.3828 1.8294 1.5013 1.5993

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
Localization
Cell Percentages mitochondrion (67%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mir1

Mir1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mir1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available