Standard name
Human Ortholog
Description Subunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0 – 0 0 0 0 0 0.08 0.13 0.15 0.12 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0.37 0.28 0.06 0.27 – 0.23 0.51 0.35 0.43 0.35 0 0 0 0.07 0.14 0 0 0 0 0 0 0
Nucleus 0.94 0.94 0.88 0.8 0.84 0.76 – 0.75 0.63 0.75 0.67 0.66 0.83 0.83 0.77 0.72 0.71 0.65 0.9 0.81 0.74 0.66 0.67 0.65
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0.13 0.19 0.23 0.13 0.27 – 0.33 0.41 0.37 0.41 0.37 0.17 0.17 0.15 0.05 0 0.1 0.07 0.12 0.14 0.22 0.23 0.18
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.12 0.11 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 0 1 – 0 2 2 2 2 0 1 0 0 1 0 1 3 2 3 2 2
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 3 4 1 8 – 13 13 15 15 22 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 7 11 18 5 – 18 7 5 3 8 28 35 58 19 6 10 1 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 3 2 5 0 0 0 1 0 0
Endosome 0 1 0 0 2 1 – 1 0 0 0 1 6 1 5 3 4 3 0 3 5 1 2 6
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 2 1 2 0 1 2 1 2 5
Mitochondria 42 11 98 70 13 96 – 95 169 150 109 111 9 2 1 11 10 4 1 1 3 0 1 2
Nucleus 291 400 233 200 178 271 – 314 210 321 171 208 292 227 303 110 49 64 281 369 196 119 163 196
Nuclear Periphery 0 2 0 2 0 8 – 4 6 5 3 7 3 0 2 2 0 0 0 0 0 0 0 1
Nucleolus 29 54 49 57 28 97 – 139 138 158 105 116 60 47 58 8 1 10 21 54 38 40 54 53
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 2 3 3 0 1 3 – 0 3 0 1 0 5 1 3 0 0 0 0 2 2 4 2 7
Vac/Vac Membrane 0 1 2 0 2 3 – 3 2 1 1 1 3 0 1 14 8 11 3 14 9 6 9 21
Unique Cell Count 308 424 264 250 213 356 421 334 430 255 314 353 275 392 153 69 98 311 454 266 181 243 301
Labelled Cell Count 366 474 396 345 244 493 587 551 658 410 477 409 315 434 172 82 109 311 454 266 181 243 301


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.0 4.8 5.2 6.4 4.7 – 5.1 4.2 4.7 4.6 4.8 6.9 6.9 7.1 8.7 8.4 8.1 6.6 7.4 7.6
Std Deviation (1e-4) 0.9 1.3 1.4 1.6 2.5 1.2 – 1.8 1.3 1.3 1.4 1.7 1.8 1.6 1.9 1.7 1.8 1.7 1.6 1.5 1.6
Intensity Change (Log2) – – – 0.12 0.42 -0.03 – 0.1 -0.19 -0.01 -0.07 0.01 0.53 0.54 0.58 0.86 0.81 0.77 0.47 0.63 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 1.6 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 2.8 -1.1 – 1.1 -0.4 0 0 -0.1 2.8 4.4 5.1 4.0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.2 -8.0 0 – -4.1 0 0 0 0 -11.2 -10.9 -12.9 -6.7 -3.6 -6.2
Nucleus -2.6 -1.5 -3.8 – -4.3 -7.0 -4.3 -5.8 -6.2 -1.9 -1.9 -3.6 -4.2 -3.5 -5.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 -1.6 0 – 4.1 0 0 0 0 -0.5 -0.4 -1.3 -3.8 -3.5 -1.9
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 4.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9073 0.642 0.4233 0.2411 -0.1548 0.7395 1.0329 0.7097 0.7895 0.2059 1.2048 0.8943 2.6097 2.1798 2.1275 2.1522 2.1587 2.0644
Actin 0.0032 0 0.0106 0 0.0277 0.0001 0.0075 0 0.001 0 0.0443 0.0002 0.014 0 0.0104 0 0 0.0014
Bud 0.0001 0 0.0001 0 0.001 0 0.0003 0 0.0001 0 0.0009 0 0 0 0 0 0 0
Bud Neck 0.0009 0.0002 0.0003 0.0001 0.0171 0.0009 0.0022 0.0002 0.0003 0.0001 0.0066 0.0006 0.0007 0.0001 0.0002 0 0.0005 0.0016
Bud Periphery 0.0002 0 0.0002 0 0.0016 0.0001 0.0011 0 0.0002 0 0.0022 0 0.0001 0 0 0 0 0.0001
Bud Site 0.0003 0.0002 0.0002 0 0.0086 0.0001 0.0008 0.0001 0.0004 0 0.0105 0 0.0002 0.0001 0.0001 0 0.0001 0.0001
Cell Periphery 0 0 0 0 0.0005 0 0.0002 0 0.0001 0 0.0004 0 0.0001 0 0 0 0 0
Cytoplasm 0.0048 0.0008 0.0001 0 0.0019 0.0004 0.0008 0.0021 0.0024 0 0.0031 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0003
Cytoplasmic Foci 0.0016 0 0.0003 0 0.0018 0 0.0023 0.0001 0.0011 0 0.0024 0.0001 0.0001 0 0.0001 0 0 0.0001
Eisosomes 0 0 0 0 0.0007 0 0.0004 0 0.0001 0 0.0004 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0.0001 0.0001 0 0.0018 0.0002 0.0012 0.0001 0.0014 0 0.003 0.0001 0.0003 0 0 0 0 0.0005
Endosome 0.0036 0.0001 0.0005 0 0.0065 0.0009 0.0065 0.0003 0.0023 0 0.0132 0.0017 0.0005 0 0.0001 0 0 0.0018
Golgi 0.0004 0 0.001 0 0.0022 0 0.003 0 0.0005 0 0.0112 0.0001 0.0003 0 0.0002 0 0 0.0005
Lipid Particles 0.0006 0 0.0008 0 0.0017 0.0001 0.0083 0 0.0045 0 0.0081 0.0002 0.0002 0 0.0001 0 0 0.0003
Mitochondria 0.0017 0.0001 0.0002 0.0003 0.0019 0.0003 0.008 0.0001 0.0011 0.0001 0.0085 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0002
None 0.0056 0.0109 0.0001 0 0.0052 0.0001 0.0009 0.0003 0.0027 0 0.0068 0.0003 0.0003 0.0001 0 0 0 0.0001
Nuclear Periphery 0.0109 0.0019 0.0009 0.0005 0.0044 0.0035 0.0075 0.0028 0.0101 0.0002 0.0323 0.0032 0.0012 0.0006 0.0002 0.0004 0.0008 0.0061
Nucleolus 0.0277 0.0275 0.0159 0.0101 0.0731 0.0471 0.0231 0.0225 0.0169 0.0101 0.0604 0.0554 0.0245 0.0412 0.0306 0.0163 0.0891 0.0614
Nucleus 0.919 0.9514 0.9664 0.9886 0.8258 0.9366 0.9139 0.9506 0.945 0.9878 0.7405 0.9307 0.9546 0.9493 0.9569 0.9827 0.9051 0.9192
Peroxisomes 0.0008 0 0.0004 0 0.0011 0 0.0024 0 0.0029 0 0.0014 0 0.0001 0 0.0002 0 0 0
Punctate Nuclear 0.0162 0.0066 0.0017 0.0001 0.0133 0.009 0.007 0.0205 0.0058 0.0016 0.0403 0.0067 0.0016 0.0083 0.0006 0.0003 0.0039 0.0051
Vacuole 0.0009 0.0001 0 0.0001 0.0015 0.0004 0.0016 0.0002 0.0008 0 0.0016 0.0001 0.0003 0 0 0 0 0.0009
Vacuole Periphery 0.0007 0 0 0.0001 0.0006 0.0002 0.0009 0.0001 0.0005 0 0.0017 0.0002 0.0001 0 0 0 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –
Standard name
Human Ortholog
Description Subunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Eaf6

Eaf6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eaf6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available