ORF
Human Ortholog
Description Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0.05 0.08 0 0 0 0.11 0.17 0.2 0.13 0.16 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.14 0 0.08 0.11 0.31 0.49 0.33 0.4 0.61 0.65 0.59 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleus 0.86 0.9 0.95 0.92 0.88 0.84 0.75 0.79 0.75 0.76 0.75 0.76 0.88 0.82 0.78 0.71 0.71 0.69 0.79 0.87 0.83 0.78 0.66 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.15 0.17 0.14 0.07 0.09 0.11 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud 1 0 0 0 1 4 7 3 6 3 4 4 0 0 0 2 0 0 3 1 1 2 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 3
Bud Site 1 0 0 0 0 3 8 12 4 18 7 6 0 0 0 0 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 2 5 8 13 16 15 21 17 15 8 8 29 88 93 26 38 21 1 1 1 10 17 13
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 14 8 5 0 0 0 0 0 1
Endosome 3 0 1 0 3 1 2 3 2 0 0 0 0 1 0 7 4 2 1 0 0 3 6 6
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 2 0 0 0 0 0 1 4
Mitochondria 121 16 5 12 25 118 247 144 87 250 149 155 2 0 0 4 19 6 5 0 3 4 13 15
Nucleus 244 103 176 146 200 315 380 344 162 308 173 200 236 413 363 142 174 110 248 104 173 280 305 326
Nuclear Periphery 4 0 1 0 2 2 3 3 4 3 5 5 0 0 1 0 0 0 0 0 0 0 0 2
Nucleolus 5 0 0 0 1 4 18 11 2 5 14 9 0 0 0 0 0 0 0 1 1 5 15 22
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 4 0 1 0 0 0 1 6 1 0 0 1 0 5 1 1 0 2 2 0 2 8 14 21
Vac/Vac Membrane 6 7 3 5 4 10 17 8 8 9 10 3 2 11 12 20 36 27 43 7 19 39 72 104
Unique Cell Count 284 114 186 159 226 377 507 434 217 407 231 263 269 506 465 199 245 160 315 119 208 358 463 541
Labelled Cell Count 397 128 192 171 249 473 699 555 294 611 370 391 269 518 471 220 282 174 315 119 208 358 463 541


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.7 5.2 5.0 4.7 4.2 4.0 4.1 4.1 3.7 3.7 3.5 6.2 6.3 6.2 6.1 6.1 6.4 6.1 6.3 6.5
Std Deviation (1e-4) 0.5 0.7 1.4 2.5 1.3 1.6 1.3 1.6 1.6 0.8 1.3 0.9 1.0 1.1 1.1 1.2 1.5 1.4 1.1 1.2 1.3
Intensity Change (Log2) – – – -0.06 -0.14 -0.29 -0.37 -0.35 -0.32 -0.48 -0.48 -0.55 0.27 0.28 0.27 0.25 0.24 0.3 0.24 0.28 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.5 4.7 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2576 1.845 1.4236 1.6821 1.0996 1.5933 0.5389 0.1659 0.1947 -0.0016 0.6598 0.2625 0.2725 0.5557 0.2044 0.0649 -0.4513 0.1807
Actin 0.0002 0.0001 0.0029 0.0001 0.0001 0.0001 0.065 0.0001 0.0009 0.0019 0.0009 0.0083 0.0055 0 0.0065 0.0153 0.0001 0.0099
Bud 0 0 0.0001 0 0.0001 0.0001 0.0007 0.0001 0.0005 0.0008 0.0002 0.0006 0.0005 0 0.0012 0.0166 0.0001 0.0046
Bud Neck 0.0002 0.0002 0.0003 0.0001 0.0032 0.0028 0.0084 0.0003 0.0028 0.0015 0.0014 0.0028 0.0017 0.0001 0.0008 0.0003 0.0008 0.0017
Bud Periphery 0 0 0.0003 0 0.0005 0.0001 0.0015 0.0001 0.0013 0.0032 0.0002 0.0009 0.0006 0 0.001 0.0047 0 0.0013
Bud Site 0.0002 0.0002 0.0002 0 0.0002 0.0001 0.0048 0.0004 0.0054 0.0017 0.0008 0.0007 0.0024 0.0002 0.0084 0.0026 0.0001 0.0007
Cell Periphery 0.0001 0 0 0 0.0002 0 0.0005 0 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0002 0.0002 0 0.0001
Cytoplasm 0.0208 0.0056 0.0094 0.0032 0.0088 0.0107 0.0469 0.0225 0.0123 0.0038 0.021 0.012 0.037 0.0097 0.0201 0.0042 0.0091 0.0087
Cytoplasmic Foci 0.0004 0.0001 0.0008 0 0.0007 0.0051 0.0252 0.0004 0.0059 0.0016 0.0038 0.0063 0.0309 0.01 0.0039 0.0024 0.0028 0.0062
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0001
Endoplasmic Reticulum 0.0011 0.0002 0.001 0.0001 0.0002 0.0004 0.0064 0.0021 0.0013 0.0006 0.0014 0.0012 0.0031 0.0003 0.0026 0.0004 0.0002 0.0018
Endosome 0.0007 0.0002 0.0026 0.0001 0.0003 0.0004 0.0334 0.001 0.0032 0.0163 0.0421 0.0092 0.0281 0.0011 0.0097 0.0058 0.0005 0.0075
Golgi 0 0 0.0003 0 0 0 0.0125 0 0.0011 0.0305 0.0322 0.0052 0.0081 0.0001 0.0015 0.0052 0 0.004
Lipid Particles 0.0001 0 0.0008 0 0.0001 0.0001 0.0334 0.0001 0.0024 0.0006 0.0066 0.01 0.0169 0.0004 0.0015 0.0037 0.0001 0.0061
Mitochondria 0.0005 0.0002 0.0043 0.0003 0.0004 0.0012 0.0166 0.0004 0.0013 0.0089 0.0062 0.018 0.005 0.0003 0.0016 0.0284 0.0002 0.0032
None 0.0937 0.0349 0.0332 0.0002 0.0422 0.0338 0.0172 0.0081 0.0013 0.0006 0.042 0.0148 0.0329 0.0221 0.0226 0.0035 0.0019 0.0027
Nuclear Periphery 0.0133 0.0239 0.0202 0.0093 0.0066 0.0031 0.0235 0.0192 0.0146 0.0382 0.0115 0.0087 0.028 0.011 0.0153 0.0033 0.007 0.0116
Nucleolus 0.0061 0.0065 0.0057 0.0028 0.0103 0.0155 0.0093 0.0073 0.0063 0.0143 0.0163 0.0158 0.0182 0.0036 0.0042 0.0061 0.0111 0.0113
Nucleus 0.8431 0.9078 0.8955 0.9816 0.9155 0.898 0.6327 0.926 0.9217 0.8674 0.7935 0.8461 0.7294 0.9378 0.8759 0.8538 0.8764 0.9014
Peroxisomes 0 0 0.0002 0 0 0.0001 0.0254 0 0.0021 0.0003 0.0004 0.0062 0.0172 0 0.0069 0.0028 0 0.0013
Punctate Nuclear 0.0182 0.0193 0.0209 0.0017 0.0089 0.028 0.0212 0.0104 0.0109 0.0037 0.0162 0.0288 0.0296 0.0027 0.013 0.0387 0.0892 0.014
Vacuole 0.0009 0.0003 0.0006 0.0001 0.0015 0.0004 0.0097 0.0011 0.0037 0.0023 0.002 0.0031 0.0027 0.0004 0.0022 0.0011 0.0002 0.0009
Vacuole Periphery 0.0002 0.0003 0.0006 0.0001 0.0002 0.0001 0.0052 0.0003 0.0009 0.0014 0.0014 0.001 0.0018 0.0002 0.0009 0.0007 0.0001 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7107 13.9582 9.7821 11.4562 14.0587 11.2707 13.133 10.0546 11.0493 11.3451
Translational Efficiency 0.95 0.6111 0.8145 0.7616 0.6032 0.6652 0.7555 0.7983 0.7849 0.7676

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YJR084W

YJR084W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr084w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available