Standard name
Human Ortholog
Description Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.07 0.14 0.08 0.08 0.05 0.09 0.07 0.08 0.05 0.07 0 0.06 0.07 0.08 0.14 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0.08 0.06 0.05 0 0.07 0.06 0.06 0 0.05 0.07 0.19 0.23 0.26 0.26 0.28 0 0 0 0 0 0
Cytoplasm 0.05 0.12 0.05 0.07 0.09 0.11 0.11 0.11 0.11 0.11 0.15 0.14 0 0.07 0 0.17 0.17 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0.94 0.87 0.93 0.82 0.85 0.77 0.69 0.59 0.69 0.64 0.61 0.58 0.88 0.84 0.82 0 0 0 0.93 0.88 0.92 0.89 0.9 0.85
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.12 0.1 0.11 0 0 0 0 0 0 0 0.05 0 0 0.16 0.15 0.12 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.11 0.09 0.11 0.15 0.12 0.24 0.18 0.26 0.15 0 0 0 0.06 0.1 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.13 0.18 0.09 0.13 0.12 0.16 0 0 0 0.47 0.31 0.38 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 22 14 24 21 18 18 26 17 30 10 23 4 18 21 14 15 14 1 6 5 5 5 5
Bud 1 2 0 1 1 1 3 1 5 10 2 2 2 0 0 0 0 0 0 2 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 3 7 9 13 15 12 10 20 13 23 7 15 8 55 67 45 27 39 0 0 1 1 3 7
Cytoplasm 10 35 9 12 22 26 37 32 25 42 28 44 4 19 13 29 18 26 2 12 1 1 4 3
Endoplasmic Reticulum 189 258 174 137 215 178 226 175 159 252 112 179 95 237 242 4 4 2 202 287 176 149 239 277
Endosome 0 0 1 0 0 0 1 2 1 0 0 2 0 0 0 1 1 0 0 0 0 0 0 0
Golgi 5 3 23 16 29 2 4 13 1 6 0 8 5 4 5 27 16 17 0 2 0 0 1 5
Mitochondria 2 1 12 18 22 25 48 37 55 70 47 45 0 0 2 11 10 19 3 4 3 1 4 9
Nucleus 0 1 0 0 1 1 2 0 0 1 2 1 1 1 1 0 1 2 0 0 0 0 0 0
Nuclear Periphery 2 2 11 9 4 5 9 9 14 16 8 12 4 2 2 0 0 2 1 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 8 4 3 8 14 43 53 21 52 23 48 2 0 5 80 32 53 3 7 0 2 4 5
Unique Cell Count 200 298 187 168 253 232 328 299 232 396 184 308 108 283 296 170 104 139 218 328 192 167 267 325
Labelled Cell Count 218 339 257 233 338 282 401 368 313 502 240 379 125 336 358 211 125 174 218 328 192 167 267 325


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.7 16.4 17.5 17.0 15.6 13.1 12.3 13.7 12.5 12.5 12.0 12.3 17.2 17.2 17.5 13.8 13.8 13.9 15.8 15.8 17.0
Std Deviation (1e-4) 2.9 2.8 3.0 2.7 2.8 2.5 2.3 2.8 2.8 2.5 2.6 2.9 3.2 2.9 2.7 5.4 5.4 5.3 2.5 2.7 2.9
Intensity Change (Log2) -0.04 -0.17 -0.42 -0.51 -0.35 -0.49 -0.48 -0.55 -0.51 -0.03 -0.03 -0.0 -0.34 -0.34 -0.33 -0.15 -0.15 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.1 0.3 0.1 -0.9 0.5 -0.1 0 -0.8 0 -1.3 -0.5 -0.2 0.3 1.9 0.8
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 0.5 0.2 -1.0 0.8 0.4 0.5 -0.5 0 0.9 4.5 5.2 5.7 5.3 5.9
Cytoplasm 0.9 1.6 2.4 2.5 2.3 2.2 2.3 3.3 3.3 0 0.9 -0.2 3.7 3.5 4.0
Endoplasmic Reticulum -3.3 -2.6 -4.5 -6.3 -8.2 -6.2 -7.5 -7.4 -8.3 -1.5 -3.0 -3.5 -17.1 -15.0 -16.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.8 -0.3 -4.9 -5.4 -3.3 -5.2 -5.6 -4.9 -4.3 -2.2 -5.0 -4.9 1.0 0.7 0
Mitochondria 1.5 0.9 1.6 2.8 2.1 4.8 3.7 5.0 2.8 0 0 -3.7 0 1.0 2.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.2 -2.5 -2.0 -1.8 -1.6 0.1 -1.0 -0.7 -1.0 -0.8 -3.3 -3.4 -3.2 0 -2.0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.7 2.0 4.2 5.2 3.0 4.2 3.8 4.7 0 0 0 10.0 7.1 8.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.4041 11.0448 11.3515 9.9678 11.2468 10.8605 13.6691 14.4624 13.1252 12.1436 10.6418 13.5536 9.749 13.6203 10.5922 10.7434 9.552 11.0576
Actin 0.0093 0.0133 0.0015 0.0008 0.0014 0.0011 0.0181 0.0001 0.0101 0.0032 0.0388 0.0017 0.0396 0.0011 0.0014 0 0.0086 0.0003
Bud 0 0.0002 0.0002 0 0 0 0.0003 0 0.0003 0.0002 0.0002 0 0.0007 0 0 0 0.0003 0
Bud Neck 0.0001 0.0012 0.0007 0.0002 0.0004 0.0006 0.0004 0.0001 0.0018 0.0012 0.0003 0.0006 0.0009 0.0003 0.0001 0 0.0005 0.0004
Bud Periphery 0.0001 0.0006 0.0005 0.0001 0 0.0002 0.0003 0 0.0005 0.001 0.0004 0.0001 0.0008 0 0.0001 0 0.0003 0
Bud Site 0.0001 0.0034 0.0004 0 0 0 0.004 0 0.0014 0.0009 0.0008 0 0.0128 0.0002 0 0 0.0006 0
Cell Periphery 0.0018 0.0149 0.0044 0.0017 0.0007 0.0012 0.001 0.0021 0.0011 0.0041 0.0008 0.0004 0.0012 0.0029 0.0014 0.0008 0.0002 0.0006
Cytoplasm 0.0109 0.0373 0.0153 0.004 0.0029 0.0054 0.0103 0.0061 0.0034 0.002 0.0021 0.007 0.0085 0.1013 0.0078 0.001 0.0025 0.0018
Cytoplasmic Foci 0.002 0.002 0.0006 0.0015 0.001 0.0003 0.0081 0.0003 0.0033 0.0014 0.0071 0.0038 0.0082 0.0025 0.0009 0.0001 0.0113 0.0002
Eisosomes 0.0001 0.0008 0.0002 0.0001 0 0 0.0001 0.0001 0.0003 0.0006 0.0004 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.9102 0.8855 0.9596 0.9319 0.9625 0.9663 0.8987 0.9813 0.9364 0.924 0.8328 0.9522 0.8025 0.8629 0.949 0.9918 0.7903 0.9823
Endosome 0.0054 0.0048 0.0023 0.0045 0.0128 0.0051 0.0164 0.0007 0.0096 0.008 0.0276 0.0069 0.0426 0.0032 0.0068 0.0009 0.0607 0.0019
Golgi 0.0017 0.0063 0.0013 0.0024 0.0055 0.0012 0.0046 0.0016 0.0049 0.0057 0.0289 0.0046 0.0239 0.0008 0.0215 0.0019 0.0839 0.0006
Lipid Particles 0.0024 0.0143 0.002 0.0231 0.0007 0.0014 0.0108 0.004 0.0034 0.0106 0.0077 0.0027 0.0098 0.0093 0.0005 0.0003 0.0013 0.0005
Mitochondria 0.0002 0.0032 0.0006 0.0002 0.0003 0.0002 0.0007 0 0.0146 0.0252 0.0162 0.0013 0.0068 0 0.0015 0.0001 0.0299 0.0001
None 0.0007 0.0004 0.0004 0.0003 0.0001 0.0001 0.0012 0 0.0002 0.0002 0.001 0.0048 0.0012 0.0001 0 0 0.0001 0
Nuclear Periphery 0.036 0.0071 0.0061 0.0258 0.0097 0.0129 0.012 0.0024 0.0037 0.0031 0.0093 0.0071 0.0239 0.0089 0.0064 0.0019 0.0053 0.0076
Nucleolus 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0.0002 0.0001 0 0.0006 0 0 0 0 0
Nucleus 0.0077 0.0008 0.0005 0.0007 0.0002 0.0006 0.0006 0.0001 0.0003 0.0003 0.0005 0.0004 0.0063 0.0006 0.0002 0 0.0002 0.0003
Peroxisomes 0.0049 0.0001 0 0 0 0 0.0074 0 0.0005 0.0006 0.0205 0.0032 0.0016 0 0 0 0.0006 0
Punctate Nuclear 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0007 0 0.0004 0.0001 0.0005 0.0003 0.0038 0.0002 0 0 0.0001 0
Vacuole 0.0045 0.003 0.0029 0.0016 0.0012 0.0028 0.0029 0.001 0.003 0.0059 0.0018 0.002 0.0029 0.0054 0.0017 0.0008 0.0012 0.0028
Vacuole Periphery 0.0012 0.0005 0.0006 0.0007 0.0005 0.0006 0.0011 0.0001 0.0007 0.0014 0.0022 0.0008 0.0014 0.0003 0.0006 0.0001 0.0019 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.0736 42.642 42.4671 53.8511 59.4054 37.638 48.7883 55.5195 68.9542 54.9252
Translational Efficiency 1.7203 2.0105 2.0736 2.1407 1.4193 1.9603 1.9628 1.9062 1.3424 1.4656

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Emc2

Emc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available