Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.06 0.09 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.08 – 0.05 0.09 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.95 0.96 0.94 0.88 0.77 – 0.7 0.56 0.69 0.63 0.97 0.98 0.97 0.99 0.99 0.99 0.95 0.97 0.92 0.9 0.9 0.89
Endoplasmic Reticulum 0 0 0.05 0 0.06 0.06 0.15 – 0.18 0.21 0.11 0.16 0.05 0 0 0 0 0.06 0 0 0 0.05 0.06 0.06
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0.1 0.17 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 – 0.05 0.07 0.16 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 5 12 – 17 34 16 20 1 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 2 0 0 – 0 1 0 1 0 0 1 0 0 0 2 1 0 1 6 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 7 2 4 12 8 29 – 14 34 13 19 5 3 3 1 5 3 0 0 1 0 0 1
Cytoplasm 508 584 224 214 290 187 269 – 185 219 125 139 202 377 288 205 165 183 493 573 217 188 407 342
Endoplasmic Reticulum 10 13 11 8 18 12 52 – 48 84 20 35 10 17 9 5 1 12 11 3 8 11 25 23
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 2 1 1 3 11 – 27 65 14 23 0 1 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 12 2 – 14 29 29 30 0 0 0 2 1 1 1 0 2 0 1 0
Nucleus 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 1 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 4 – 11 8 4 6 0 0 1 0 2 0 0 4 1 2 3 5
Unique Cell Count 518 592 237 224 310 212 350 265 391 181 220 209 386 296 208 167 185 517 590 236 210 454 383
Labelled Cell Count 522 604 239 228 325 228 379 316 475 221 273 218 398 302 213 174 200 517 590 236 210 454 383


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.8 63.3 53.8 48.6 43.9 23.5 27.5 – 22.4 21.8 19.7 19.3 63.2 57.1 61.5 89.4 96.5 93.5 60.5 65.6 69.6
Std Deviation (1e-4) 13.4 11.8 10.2 10.6 7.5 6.7 6.5 – 5.2 5.0 5.1 4.4 14.4 12.8 14.4 27.6 23.5 26.1 15.4 18.1 17.8
Intensity Change (Log2) – – – -0.15 -0.29 -1.19 -0.97 – -1.26 -1.3 -1.45 -1.48 0.23 0.09 0.19 0.73 0.84 0.8 0.17 0.29 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 4.0 4.7 4.7 4.7 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 0 4.0 – 2.8 4.1 3.5 4.0 0 0 0 0 0 0
Cytoplasm 0.5 -0.5 -2.4 -5.7 – -7.1 -10.3 -6.9 -8.3 1.1 2.1 1.6 2.3 2.2 2.4
Endoplasmic Reticulum -0.6 0.6 0.5 3.9 – 4.7 5.7 2.5 4.0 0.1 -0.1 -1.0 -1.3 0 0.8
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1.9 – 4.5 6.2 3.6 4.5 0 0 0 0 0 0
Mitochondria 0 0 3.7 0 – 3.6 4.3 6.4 5.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 3.2 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 56.6791 72.9946 64.4808 57.6636 58.586 62.3292 70.2891 88.7244 82.0513 74.3624 76.5284 79.7771 73.0899 92.4236 80.952 77.4861 71.9922 84.3816
Actin 0.0034 0.0011 0.0035 0.0354 0.0491 0.001 0.0019 0.0002 0.0003 0.0111 0.0003 0.0015 0.0145 0.0006 0.0014 0.0009 0.0011 0.0015
Bud 0.0014 0.0005 0.0017 0.0023 0.0012 0.0015 0.0012 0.0002 0.0007 0.0011 0.0008 0.001 0.002 0.001 0.0031 0.0066 0.0033 0.0015
Bud Neck 0.0016 0.0005 0.0013 0.0015 0.0014 0.0015 0.0005 0.0003 0.0008 0.0012 0.0006 0.0014 0.0009 0.0009 0.0012 0.0011 0.0013 0.0015
Bud Periphery 0.0024 0.0003 0.0019 0.0019 0.0018 0.0008 0.0015 0.0002 0.0005 0.001 0.0005 0.0013 0.0042 0.0007 0.0039 0.005 0.0034 0.0008
Bud Site 0.0023 0.0004 0.01 0.0088 0.0011 0.0002 0.0006 0.0002 0.0003 0.0027 0.0004 0.0001 0.0016 0.0007 0.0012 0.001 0.0005 0.0003
Cell Periphery 0.0153 0.0088 0.0053 0.0066 0.0467 0.0021 0.0208 0.0081 0.0098 0.0033 0.0059 0.004 0.0142 0.0081 0.0221 0.0063 0.0094 0.0012
Cytoplasm 0.6819 0.9284 0.8313 0.6156 0.7113 0.744 0.7354 0.9377 0.8577 0.7485 0.7596 0.8066 0.6835 0.9124 0.7368 0.514 0.5661 0.8153
Cytoplasmic Foci 0.0108 0.0013 0.0054 0.0053 0.0058 0.0062 0.0074 0.0013 0.0045 0.0031 0.0067 0.0036 0.0078 0.0016 0.0041 0.0022 0.0068 0.0048
Eisosomes 0.0002 0 0.0001 0.0008 0.0001 0 0.0002 0 0 0.0001 0 0.0001 0.0002 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0738 0.0133 0.0229 0.0391 0.0115 0.0491 0.0794 0.0122 0.0443 0.0463 0.0702 0.1033 0.0623 0.0101 0.0154 0.0115 0.0711 0.0116
Endosome 0.0248 0.0018 0.0118 0.016 0.0195 0.0378 0.0127 0.0027 0.0077 0.012 0.0278 0.0145 0.0171 0.0039 0.008 0.0483 0.0331 0.0308
Golgi 0.0033 0.0002 0.0015 0.006 0.005 0.0142 0.0021 0.0005 0.0027 0.0014 0.0036 0.0063 0.0024 0.0008 0.0013 0.0028 0.0055 0.0076
Lipid Particles 0.0046 0.0007 0.0016 0.0038 0.0031 0.0006 0.0033 0.0025 0.0017 0.0027 0.0011 0.0005 0.0032 0.0005 0.0025 0.0007 0.0029 0.0008
Mitochondria 0.009 0.0002 0.0032 0.0204 0.0009 0.0108 0.0082 0.0003 0.0013 0.0026 0.0046 0.0026 0.0076 0.0013 0.0184 0.0018 0.0169 0.0072
None 0.0037 0.0002 0.0011 0.0206 0.0002 0.0016 0.0038 0.0002 0.0011 0.0011 0.0034 0.0004 0.0073 0.0004 0.0026 0.0003 0.0065 0.0007
Nuclear Periphery 0.029 0.0023 0.0078 0.0148 0.0067 0.0142 0.0138 0.0021 0.0038 0.0083 0.0052 0.0071 0.0289 0.0042 0.0078 0.0117 0.0114 0.0083
Nucleolus 0.003 0.0027 0.001 0.0018 0.0002 0.0016 0.0051 0.0001 0.0019 0.0004 0.0045 0.0001 0.0031 0.001 0.0027 0.0002 0.0011 0.0003
Nucleus 0.0104 0.0021 0.0048 0.0054 0.0024 0.0049 0.0058 0.0009 0.002 0.0032 0.0047 0.0013 0.0096 0.0067 0.0065 0.0046 0.0069 0.0055
Peroxisomes 0.001 0 0.0002 0.002 0.0003 0.0002 0.0004 0 0.0001 0.0003 0.0002 0 0.0005 0.0001 0.0003 0.0001 0.0003 0.0001
Punctate Nuclear 0.0031 0.0008 0.0068 0.0018 0.0001 0.0001 0.0009 0 0.0001 0.0021 0.0001 0 0.0013 0.0002 0.0003 0.0001 0.0006 0.0002
Vacuole 0.095 0.0326 0.0674 0.1606 0.1218 0.0697 0.0787 0.028 0.053 0.1197 0.074 0.0364 0.1021 0.04 0.1346 0.2824 0.1792 0.0671
Vacuole Periphery 0.02 0.0016 0.0094 0.0294 0.0099 0.0378 0.0163 0.0021 0.0056 0.0279 0.0258 0.0079 0.0259 0.0047 0.0255 0.0985 0.0726 0.0328

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1330.8086 1343.4514 1770.8073 1458.8979 1384.4633 1059.3143 1275.252 1252.1599 1369.5738 1302.7697
Translational Efficiency 1.6931 2.0414 1.6226 1.898 2.0643 2.3741 1.9877 1.8301 1.75 1.9423

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl43b

Rpl43b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl43b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available