Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 2 | 2 | 4 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 303 | 20 | 65 | 186 | 272 | 253 | 511 | – | 305 | 359 | 310 | 281 | 123 | 333 | 315 | 220 | 223 | 204 | 293 | 52 | 89 | 112 | 291 | 273 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 11 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 10 | 0 | 0 | 4 | – | 1 | 0 | 3 | 4 | 0 | 3 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 0 | 0 | 4 | 5 | 36 | 28 | – | 110 | 115 | 155 | 119 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleus | 2 | 0 | 2 | 8 | 8 | 6 | 14 | – | 14 | 11 | 7 | 4 | 3 | 2 | 2 | 5 | 5 | 4 | 17 | 6 | 3 | 3 | 10 | 7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 28 | 0 | 6 | 109 | 69 | 8 | 69 | – | 29 | 19 | 21 | 18 | 9 | 84 | 68 | 48 | 93 | 54 | 4 | 4 | 1 | 1 | 4 | 7 |
Unique Cell Count | 321 | 20 | 72 | 272 | 325 | 282 | 577 | 385 | 440 | 429 | 375 | 132 | 385 | 344 | 234 | 269 | 230 | 328 | 66 | 102 | 119 | 316 | 298 | |
Labelled Cell Count | 337 | 20 | 73 | 318 | 356 | 306 | 633 | 464 | 513 | 505 | 434 | 136 | 424 | 386 | 284 | 327 | 268 | 328 | 66 | 102 | 119 | 316 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 7.2 | 6.9 | 7.9 | 7.3 | 4.6 | 5.4 | – | 4.4 | 4.5 | 4.3 | 4.4 | 8.6 | 7.8 | 7.9 | 13.2 | 13.3 | 13.0 | 8.1 | 7.9 | 7.5 |
Std Deviation (1e-4) | 1.1 | 0.7 | 0.9 | 1.2 | 1.5 | 1.4 | 1.5 | – | 1.7 | 0.9 | 0.7 | 1.0 | 1.3 | 1.4 | 1.6 | 2.1 | 2.0 | 2.1 | 1.3 | 1.4 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.2 | 0.08 | -0.58 | -0.35 | – | -0.64 | -0.63 | -0.67 | -0.64 | 0.32 | 0.17 | 0.2 | 0.93 | 0.94 | 0.91 | 0.22 | 0.2 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.7 | -1.4 | -0.1 | -0.4 | – | -2.2 | -1.8 | -3.3 | -2.9 | 0.7 | -0.9 | 0.4 | 1.1 | -1.5 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 5.1 | 2.5 | 0 | 0.9 | – | -0.2 | 0 | 0 | 0 | -0.4 | 2.6 | 2.3 | 2.4 | 4.4 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2471 | 4.352 | 3.776 | 3.2763 | 3.1808 | 3.6465 | 4.4728 | 6.424 | 5.9523 | 5.4737 | 5.4145 | 5.7645 | 5.8845 | 7.7737 | 6.7479 | 5.8919 | 6.4389 | 6.7595 |
Actin | 0.0261 | 0.0002 | 0.0006 | 0.041 | 0.0085 | 0.0007 | 0.0197 | 0.0027 | 0.0093 | 0.0059 | 0.0038 | 0.0014 | 0.0095 | 0.0004 | 0.0014 | 0.0004 | 0.0002 | 0.0004 |
Bud | 0.0003 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0013 | 0.0042 | 0.0013 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0003 |
Bud Neck | 0.0047 | 0.0003 | 0.0006 | 0.0013 | 0.001 | 0.0022 | 0.0033 | 0.0003 | 0.0013 | 0.0012 | 0.0011 | 0.0022 | 0.0006 | 0.0007 | 0.0005 | 0.0009 | 0.0008 | 0.0021 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0011 | 0.0008 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0014 | 0.0004 | 0.0003 | 0.0013 | 0.0003 | 0.0001 | 0.0094 | 0.0025 | 0.0027 | 0.0007 | 0.0002 | 0.0001 | 0.0009 | 0.0019 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.7048 | 0.8825 | 0.8443 | 0.7165 | 0.7967 | 0.7525 | 0.6751 | 0.8563 | 0.7408 | 0.721 | 0.7474 | 0.6969 | 0.8459 | 0.8742 | 0.8756 | 0.8378 | 0.8434 | 0.8 |
Cytoplasmic Foci | 0.0201 | 0.0043 | 0.0054 | 0.0123 | 0.0102 | 0.0029 | 0.0234 | 0.0015 | 0.0082 | 0.0041 | 0.0053 | 0.0061 | 0.0082 | 0.0022 | 0.002 | 0.0018 | 0.0018 | 0.0042 |
Eisosomes | 0.0002 | 0 | 0 | 0.0005 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0111 | 0.0025 | 0.004 | 0.0084 | 0.0049 | 0.003 | 0.0073 | 0.0016 | 0.0021 | 0.0036 | 0.0033 | 0.0027 | 0.003 | 0.0021 | 0.0019 | 0.0034 | 0.0013 | 0.0027 |
Endosome | 0.0291 | 0.001 | 0.0043 | 0.0153 | 0.016 | 0.0037 | 0.0362 | 0.0009 | 0.0048 | 0.0126 | 0.0195 | 0.0069 | 0.01 | 0.0009 | 0.0006 | 0.0031 | 0.0007 | 0.0021 |
Golgi | 0.0049 | 0 | 0.0002 | 0.0022 | 0.0016 | 0.0001 | 0.0092 | 0.0001 | 0.0021 | 0.0058 | 0.0025 | 0.0011 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0073 | 0 | 0.0014 | 0.0023 | 0.002 | 0.0001 | 0.011 | 0.0002 | 0.002 | 0.0105 | 0.009 | 0.0028 | 0.0016 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0019 | 0.0001 | 0.0003 | 0.0023 | 0.0007 | 0.0005 | 0.0121 | 0.0038 | 0.0114 | 0.0089 | 0.0118 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 |
None | 0.0367 | 0.019 | 0.009 | 0.0282 | 0.006 | 0.0076 | 0.0164 | 0.002 | 0.0036 | 0.0028 | 0.0024 | 0.002 | 0.0105 | 0.0043 | 0.0072 | 0.0023 | 0.0031 | 0.0133 |
Nuclear Periphery | 0.0295 | 0.0035 | 0.0081 | 0.0104 | 0.0257 | 0.0135 | 0.0174 | 0.0062 | 0.0081 | 0.0164 | 0.009 | 0.0104 | 0.0058 | 0.005 | 0.0042 | 0.007 | 0.0041 | 0.0055 |
Nucleolus | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0017 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0019 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.1032 | 0.0815 | 0.1167 | 0.1426 | 0.1075 | 0.2058 | 0.1323 | 0.1129 | 0.1936 | 0.1945 | 0.1706 | 0.2502 | 0.0925 | 0.1019 | 0.1038 | 0.1379 | 0.1384 | 0.1635 |
Peroxisomes | 0.0039 | 0 | 0.0003 | 0.0053 | 0.0011 | 0 | 0.0039 | 0.0005 | 0.0017 | 0.0044 | 0.008 | 0.0037 | 0.0016 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0091 | 0.0035 | 0.0028 | 0.0051 | 0.015 | 0.0046 | 0.0111 | 0.0019 | 0.0044 | 0.0037 | 0.0033 | 0.011 | 0.005 | 0.0032 | 0.0014 | 0.0023 | 0.0052 | 0.0044 |
Vacuole | 0.003 | 0.0007 | 0.0011 | 0.0024 | 0.0012 | 0.0019 | 0.0058 | 0.0009 | 0.0011 | 0.0015 | 0.0017 | 0.0012 | 0.001 | 0.001 | 0.0004 | 0.0018 | 0.0003 | 0.0007 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0002 | 0.0006 | 0.0006 | 0.0005 | 0.0016 | 0.0002 | 0.0004 | 0.001 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.6931 | 13.3037 | 2.8206 | 10.8872 | 13.7824 | 10.6242 | 11.0493 | 16.2121 | 7.7856 | 14.8069 |
Translational Efficiency | 3.4579 | 2.3701 | 9.0603 | 1.5486 | 2.1169 | 2.8604 | 1.8357 | 1.2407 | 1.9934 | 2.3041 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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