Standard name | |
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Human Ortholog | |
Description | Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 45 | 22 | 6 | 6 | 5 | 8 | 12 | 3 | 9 | 14 | 17 | 9 | 9 | 5 | 86 | 45 | 55 | 15 | 7 | 2 | 11 | 15 | 13 |
Cytoplasm | 26 | 232 | 193 | 152 | 234 | 238 | 280 | 316 | 209 | 190 | 157 | 200 | 116 | 133 | 145 | 125 | 57 | 101 | 52 | 362 | 256 | 100 | 107 | 141 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 4 | 6 | 11 | 10 | 12 | 0 | 0 | 0 | 2 | 4 | 7 | 0 | 2 | 2 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 |
Unique Cell Count | 38 | 260 | 204 | 152 | 235 | 241 | 296 | 328 | 218 | 206 | 174 | 222 | 121 | 138 | 148 | 195 | 90 | 144 | 71 | 384 | 269 | 118 | 132 | 163 |
Labelled Cell Count | 40 | 277 | 215 | 158 | 240 | 245 | 301 | 335 | 219 | 211 | 184 | 236 | 125 | 142 | 150 | 213 | 106 | 163 | 71 | 384 | 269 | 118 | 132 | 163 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 642.1 | 498.2 | 433.9 | 362.6 | 319.5 | 245.7 | 199.6 | 171.8 | 150.5 | 130.8 | 118.8 | 105.8 | 496.7 | 467.1 | 422.3 | 603.9 | 594.6 | 592.0 | 551.4 | 559.2 | 552.9 |
Std Deviation (1e-4) | 60.8 | 90.0 | 83.2 | 74.9 | 64.8 | 64.3 | 52.6 | 45.6 | 42.0 | 45.1 | 43.0 | 42.3 | 84.6 | 90.0 | 77.0 | 127.1 | 149.8 | 138.4 | 160.5 | 159.0 | 124.2 |
Intensity Change (Log2) | – | – | – | -0.26 | -0.44 | -0.82 | -1.12 | -1.34 | -1.53 | -1.73 | -1.87 | -2.04 | 0.19 | 0.11 | -0.04 | 0.48 | 0.45 | 0.45 | 0.35 | 0.37 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.4 | -3.5 | -3.8 | -3.7 | -3.3 | -4.1 | -2.5 | -0.9 | -1.1 | -1.0 | -1.3 | -2.6 | 7.5 | 7.4 | 6.1 |
Cytoplasm | 2.9 | 3.2 | 2.5 | 0 | 1.0 | 0.6 | -1.0 | -1.6 | -1.7 | 0.5 | 0.8 | 1.6 | -7.6 | -6.9 | -6.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 586.5545 | 871.3488 | 642.5129 | 760.4482 | 405.8297 | 238.906 | 433.8122 | 697.7408 | 580.1685 | 387.145 | 486.4264 | 586.7285 | 605.5671 | 828.3716 | 710.1399 | 541.9143 | 596.3872 | 630.6699 |
Actin | 0.0231 | 0.0002 | 0.0027 | 0.0001 | 0.1055 | 0.0054 | 0.0041 | 0.0001 | 0.004 | 0.0003 | 0.0002 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0002 |
Bud | 0.0016 | 0.0004 | 0.0007 | 0.0001 | 0.0009 | 0.0098 | 0.0015 | 0.0003 | 0.0008 | 0.0006 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.008 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0006 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0011 |
Bud Periphery | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0006 | 0.0082 | 0.0008 | 0 | 0.0004 | 0.0005 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Site | 0.0015 | 0.0001 | 0.0006 | 0 | 0.0007 | 0.0039 | 0.0013 | 0.0001 | 0.001 | 0.0016 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0006 | 0.0018 | 0.0007 | 0.0001 | 0.0009 | 0.0171 | 0.0038 | 0.0016 | 0.0009 | 0.0024 | 0.0013 | 0.0004 | 0.0007 | 0 | 0.0001 | 0.0006 | 0.0009 | 0.0001 |
Cytoplasm | 0.7693 | 0.9782 | 0.8228 | 0.9858 | 0.5245 | 0.4726 | 0.7593 | 0.9848 | 0.9497 | 0.6246 | 0.7779 | 0.9515 | 0.9019 | 0.9916 | 0.989 | 0.9648 | 0.7345 | 0.8921 |
Cytoplasmic Foci | 0.0334 | 0.0001 | 0.0454 | 0 | 0.0169 | 0.0421 | 0.0286 | 0.0001 | 0.0024 | 0.0414 | 0.0032 | 0.0003 | 0.0145 | 0 | 0.0001 | 0.0002 | 0.0626 | 0.0514 |
Eisosomes | 0.001 | 0 | 0.0008 | 0 | 0.0024 | 0.0003 | 0.0014 | 0.0001 | 0.0001 | 0.0037 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0 | 0.0019 | 0.0003 |
Endoplasmic Reticulum | 0.0018 | 0 | 0.0022 | 0 | 0.0015 | 0.0212 | 0.002 | 0.0001 | 0.0007 | 0.0024 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endosome | 0.0102 | 0 | 0.0037 | 0 | 0.032 | 0.0143 | 0.0087 | 0 | 0.0013 | 0.0439 | 0.0001 | 0 | 0.0031 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Golgi | 0.0059 | 0 | 0.0009 | 0 | 0.0103 | 0.0016 | 0.0047 | 0 | 0.0006 | 0.0018 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0135 | 0 | 0.006 | 0 | 0.0379 | 0.0031 | 0.0097 | 0.0001 | 0.0006 | 0.0121 | 0.0012 | 0.0001 | 0.002 | 0 | 0 | 0.0001 | 0.0055 | 0.0019 |
Mitochondria | 0.0276 | 0.0009 | 0.0104 | 0.0002 | 0.0869 | 0.0563 | 0.0177 | 0.0004 | 0.0087 | 0.0408 | 0.0104 | 0.0003 | 0.0107 | 0.0003 | 0.0005 | 0.0002 | 0.0381 | 0.01 |
None | 0.0151 | 0.0008 | 0.013 | 0.0002 | 0.0083 | 0.0539 | 0.0254 | 0.0009 | 0.0038 | 0.0376 | 0.0114 | 0.0007 | 0.0088 | 0.0002 | 0.0002 | 0.0004 | 0.0651 | 0.0025 |
Nuclear Periphery | 0.0071 | 0.0014 | 0.0059 | 0.0027 | 0.0171 | 0.0308 | 0.0087 | 0.0019 | 0.0038 | 0.0067 | 0.0065 | 0.0076 | 0.0036 | 0.0005 | 0.0013 | 0.0054 | 0.0015 | 0.0037 |
Nucleolus | 0.0278 | 0.001 | 0.0257 | 0 | 0.103 | 0.0227 | 0.0651 | 0.0006 | 0.0037 | 0.1098 | 0.1337 | 0.0013 | 0.0289 | 0 | 0 | 0.0001 | 0.0549 | 0.0154 |
Nucleus | 0.0401 | 0.0132 | 0.0285 | 0.0103 | 0.0324 | 0.0642 | 0.0412 | 0.0076 | 0.0148 | 0.0267 | 0.0402 | 0.0352 | 0.0171 | 0.0067 | 0.0071 | 0.0261 | 0.0258 | 0.018 |
Peroxisomes | 0.0133 | 0 | 0.0228 | 0 | 0.0037 | 0.0033 | 0.0034 | 0 | 0.0004 | 0.0022 | 0.0002 | 0 | 0.0008 | 0 | 0 | 0 | 0.0027 | 0.0007 |
Punctate Nuclear | 0.0036 | 0 | 0.0016 | 0 | 0.0015 | 0.0927 | 0.0023 | 0 | 0.0003 | 0.0014 | 0.0009 | 0.0003 | 0.0026 | 0 | 0 | 0.0001 | 0.0009 | 0.0002 |
Vacuole | 0.0011 | 0.0014 | 0.0024 | 0.0001 | 0.0043 | 0.0536 | 0.0058 | 0.001 | 0.0013 | 0.0311 | 0.005 | 0.0007 | 0.0006 | 0.0001 | 0.0005 | 0.0005 | 0.0024 | 0.0008 |
Vacuole Periphery | 0.0011 | 0.0003 | 0.0027 | 0.0001 | 0.0084 | 0.0149 | 0.0042 | 0.0001 | 0.0004 | 0.0082 | 0.0065 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0024 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 223.0146 | 157.7849 | 165.4863 | 200.0931 | 171.3155 | 261.4905 | 228.5348 | 219.5631 | 202.478 | 199.089 |
Translational Efficiency | 1.3813 | 1.5342 | 1.3082 | 1.1834 | 1.2213 | 1.3138 | 1.1371 | 0.869 | 0.9433 | 0.885 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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