Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which trimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 509; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; ortholog of human gene FAM86A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.99 0.98 0.95 0.83 0.75 0.73 0.62 0.7 0.68 0.68 1.0 0.99 0.99 0.99 0.99 0.99 0.9 0.92 0.87 0.82 0.81 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.1 0.17 0.31 0.48 0.45 0.59 0.47 0.56 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 3 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 1 0
Bud 0 1 2 0 3 6 9 17 8 7 22 19 0 1 0 0 0 0 0 2 1 0 3 1
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 2
Bud Site 0 0 0 0 0 1 1 0 2 4 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 4 1 0 0 0 3 1 5 1 5 3 4 0 1 7 8 4 0 0 0 0 0 0
Cytoplasm 246 249 230 228 247 396 392 481 304 408 290 431 261 317 349 135 132 94 228 240 233 121 170 225
Endoplasmic Reticulum 0 1 1 0 0 1 1 0 8 0 1 2 1 3 3 5 2 4 0 0 1 3 3 3
Endosome 1 0 0 1 0 2 0 1 0 3 1 2 0 1 0 0 0 0 4 1 2 1 2 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 1 1
Mitochondria 8 1 14 24 45 150 250 298 289 275 237 345 0 0 0 2 2 1 7 3 10 5 7 9
Nucleus 1 1 0 0 2 1 2 6 2 4 2 1 0 0 1 0 1 0 0 1 0 0 0 1
Nuclear Periphery 0 0 1 0 1 0 2 1 5 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 0 0 1 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 3 2 2 2 3 0 0 0 0 0 0 0 0 0 0 4 1
Vac/Vac Membrane 1 0 0 0 2 2 3 6 3 11 7 6 0 0 0 0 0 0 1 2 9 9 8 11
Unique Cell Count 257 253 232 233 261 477 521 657 494 587 424 634 261 320 352 136 133 95 254 261 269 148 210 266
Labelled Cell Count 263 258 249 253 302 563 664 815 628 721 568 818 266 322 354 149 145 103 254 261 269 148 210 266


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.4 3.9 4.1 3.9 3.7 3.4 3.4 3.1 3.4 3.4 3.4 6.2 5.8 5.9 6.2 6.7 6.6 7.1 6.7 6.6
Std Deviation (1e-4) 0.8 0.8 0.6 1.8 1.8 1.0 1.5 1.1 0.6 0.8 0.9 0.9 1.6 1.3 1.3 0.9 1.0 0.9 2.9 2.2 1.8
Intensity Change (Log2) – – – 0.08 0.01 -0.08 -0.21 -0.21 -0.32 -0.21 -0.2 -0.18 0.66 0.56 0.6 0.66 0.77 0.76 0.86 0.78 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -2.8 -6.3 -8.0 -8.5 -10.7 -9.2 -9.3 -9.6 1.5 -0.1 0 0.1 0.1 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.0 -4.5 -4.7 -2.1 -2.0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8272 1.4951 1.1239 0.5554 1.0838 1.3284 1.7606 2.6715 2.3004 2.0746 1.6091 2.313 -0.3423 0.1976 -0.1534 0.4525 -0.973 -0.2314
Actin 0.0061 0.0053 0.0047 0.0405 0.0238 0.0016 0.0154 0.0012 0.0027 0.0003 0.0006 0.0026 0.0298 0.0011 0.0197 0.0002 0.0001 0.0006
Bud 0.0003 0.0041 0.0022 0.0013 0.0009 0.0006 0.0003 0.0006 0.0008 0.0002 0.0001 0.0002 0.0003 0.0003 0.0032 0.0001 0.0001 0.0001
Bud Neck 0.0012 0.0005 0.0011 0.0016 0.0003 0.0013 0.0024 0.0001 0.0002 0.0002 0.0002 0.0006 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003
Bud Periphery 0.0003 0.0014 0.0042 0.0015 0.002 0.0008 0.0003 0.0006 0.0011 0.0001 0.0001 0.0001 0.0004 0.0002 0.0041 0 0.0001 0.0002
Bud Site 0.0027 0.0149 0.0034 0.0176 0.0007 0.0001 0.0012 0.0008 0.0013 0.0002 0.0002 0 0.0017 0.002 0.0137 0.0001 0 0.0003
Cell Periphery 0.0003 0.0003 0.0004 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0002 0.0001 0 0.0002 0.0002 0.0004 0 0 0
Cytoplasm 0.3398 0.4696 0.3764 0.2984 0.3104 0.451 0.3914 0.4969 0.4959 0.5791 0.5126 0.5219 0.2308 0.5961 0.5621 0.6661 0.8546 0.69
Cytoplasmic Foci 0.0207 0.0096 0.0124 0.0385 0.0092 0.0092 0.0096 0.0057 0.0153 0.0167 0.0226 0.0107 0.0592 0.0054 0.0088 0.0194 0.0037 0.0116
Eisosomes 0.0001 0.0001 0.0002 0.0012 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0034 0.0019 0.004 0.0014 0.0037 0.0014 0.0033 0.0017 0.0011 0.0013 0.0034 0.0007 0.0052 0.0016 0.002 0.0019 0.0017 0.0012
Endosome 0.0057 0.0028 0.0033 0.0062 0.011 0.0023 0.0044 0.0023 0.0058 0.0037 0.0051 0.0022 0.0497 0.0068 0.0077 0.0147 0.0025 0.0082
Golgi 0.0014 0.0005 0.0009 0.0023 0.0023 0.0002 0.0013 0.0002 0.0004 0.0003 0.0005 0.0004 0.0158 0.0053 0.0085 0.0003 0.0001 0.0038
Lipid Particles 0.0022 0.0003 0.001 0.0029 0.0014 0.0002 0.0014 0.0001 0.0004 0.0001 0.0006 0.0006 0.0473 0.0002 0.0015 0 0 0.0012
Mitochondria 0.0008 0.0005 0.001 0.0031 0.0033 0.0004 0.0005 0.0002 0.0007 0.0003 0.0004 0.0002 0.0059 0.0047 0.0284 0.0001 0.0001 0.0352
None 0.603 0.4826 0.5722 0.5586 0.6157 0.5269 0.5587 0.4867 0.4691 0.3951 0.4491 0.4558 0.5179 0.3733 0.3323 0.2936 0.1337 0.2425
Nuclear Periphery 0.0014 0.0005 0.0014 0.002 0.0075 0.0005 0.0017 0.0003 0.0006 0.0003 0.0009 0.0009 0.0023 0.0003 0.0005 0.0005 0.0005 0.0003
Nucleolus 0.0005 0.0003 0.0006 0.0009 0.0003 0.0002 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0
Nucleus 0.0013 0.0018 0.0036 0.0038 0.0016 0.0013 0.0011 0.0007 0.001 0.0005 0.0009 0.0008 0.0006 0.0004 0.0007 0.0006 0.0006 0.0018
Peroxisomes 0.003 0.0004 0.0016 0.0057 0.0011 0.0003 0.0012 0.0001 0.0007 0.0004 0.0006 0.0003 0.0241 0.0002 0.0018 0 0 0.0006
Punctate Nuclear 0.0047 0.0015 0.0024 0.0107 0.0014 0.0009 0.0019 0.0002 0.0011 0.0003 0.0011 0.0011 0.0037 0.0002 0.0003 0.0002 0.0001 0.0001
Vacuole 0.0011 0.001 0.0025 0.0013 0.0013 0.0006 0.0025 0.0011 0.0012 0.0006 0.0007 0.0005 0.0033 0.001 0.0032 0.0017 0.0017 0.0011
Vacuole Periphery 0.0002 0.0001 0.0004 0.0003 0.0013 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.001 0.0002 0.0006 0.0003 0.0003 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.8995 12.5715 7.1758 7.1433 6.3115 12.974 12.4665 13.8585 13.6068 14.033
Translational Efficiency 0.801 0.526 0.9151 0.7812 1.029 0.6078 0.5358 0.3391 0.4634 0.5963

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which trimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 509; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; ortholog of human gene FAM86A
Localization
Cell Percentages cytoplasm (76%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Efm3

Efm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available