Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 16 | 14 | 15 | 16 | 6 | 4 | 7 | 4 | 2 | 5 | 1 | 3 | 5 | 4 | 0 | 19 | 9 | 26 | 10 | 11 | 7 | 4 | 11 | 10 |
Bud | 13 | 4 | 12 | 13 | 25 | 31 | 45 | 24 | 35 | 42 | 39 | 32 | 4 | 1 | 4 | 1 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 4 | 10 | 18 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 0 | 2 | 2 | 3 | 3 | 2 | 3 | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 28 | 28 | 48 | 36 | 45 | 80 | 54 | 67 | 70 | 67 | 79 | 11 | 17 | 25 | 18 | 21 | 14 | 1 | 6 | 3 | 0 | 0 | 1 |
Endoplasmic Reticulum | 9 | 11 | 0 | 3 | 0 | 3 | 0 | 0 | 3 | 2 | 0 | 1 | 13 | 7 | 6 | 6 | 2 | 7 | 1 | 0 | 1 | 0 | 2 | 4 |
Endosome | 58 | 39 | 68 | 61 | 78 | 66 | 69 | 42 | 15 | 8 | 13 | 31 | 2 | 24 | 24 | 30 | 31 | 23 | 112 | 117 | 124 | 28 | 70 | 44 |
Golgi | 26 | 46 | 9 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 14 | 8 | 36 | 43 | 65 | 110 | 115 | 62 | 23 | 64 | 63 |
Mitochondria | 202 | 180 | 76 | 113 | 92 | 182 | 269 | 139 | 258 | 271 | 241 | 250 | 117 | 44 | 62 | 16 | 15 | 12 | 30 | 40 | 64 | 11 | 23 | 21 |
Nucleus | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 4 | 3 | 3 | 4 | 5 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 8 | 1 | 0 | 0 | 1 | 2 | 1 | 9 | 0 | 4 | 4 | 2 | 2 | 0 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 17 | 8 | 20 | 18 | 25 | 33 | 39 | 19 | 9 | 1 | 7 | 11 | 2 | 3 | 5 | 0 | 0 | 0 | 53 | 20 | 40 | 4 | 23 | 21 |
SpindlePole | 20 | 12 | 73 | 40 | 58 | 64 | 99 | 69 | 35 | 41 | 33 | 68 | 2 | 2 | 3 | 9 | 11 | 17 | 10 | 8 | 13 | 4 | 1 | 1 |
Vac/Vac Membrane | 11 | 14 | 20 | 5 | 13 | 13 | 14 | 21 | 3 | 10 | 11 | 7 | 4 | 8 | 4 | 7 | 9 | 11 | 6 | 15 | 6 | 0 | 1 | 1 |
Unique Cell Count | 298 | 273 | 242 | 257 | 262 | 331 | 469 | 289 | 345 | 358 | 324 | 396 | 142 | 105 | 125 | 108 | 109 | 135 | 344 | 346 | 335 | 79 | 204 | 174 |
Labelled Cell Count | 390 | 359 | 323 | 325 | 341 | 448 | 633 | 394 | 443 | 479 | 434 | 511 | 172 | 127 | 146 | 146 | 143 | 183 | 344 | 346 | 335 | 79 | 204 | 174 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 6.4 | 6.2 | 6.1 | 6.5 | 5.0 | 5.0 | 5.6 | 4.5 | 4.2 | 4.2 | 4.7 | 5.8 | 6.2 | 6.1 | 10.0 | 10.4 | 9.5 | 5.6 | 5.7 | 6.5 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.3 | 1.8 | 1.6 | 1.4 | 1.0 | 1.6 | 1.8 | 1.2 | 1.6 | 1.0 | 0.8 | 1.1 | 0.9 | 1.7 | 2.0 | 1.7 | 0.8 | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.05 | -0.32 | -0.33 | -0.17 | -0.48 | -0.57 | -0.57 | -0.41 | -0.11 | -0.01 | -0.03 | 0.67 | 0.74 | 0.61 | -0.15 | -0.13 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.2 | -3.3 | -3.4 | -3.0 | 0 | 0 | 0 | 0 | -1.1 | -0.9 | -2.8 | 3.3 | 0.7 | 3.9 |
Bud | 0.1 | 2.0 | 2.0 | 2.2 | 1.5 | 0 | 0 | 0 | 0 | -1.0 | 0 | -0.8 | 0 | 0 | -0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 0.7 | 0.7 | 1.9 | 2.3 | 2.5 | 2.6 | 2.9 | 2.7 | -1.2 | 1.2 | 2.2 | 1.3 | 1.9 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.1 | 0.4 | -2.3 | -4.3 | -3.8 | 0 | 0 | 0 | 0 | -6.5 | -1.0 | -1.9 | -0.1 | 0.1 | -2.4 |
Golgi | -1.5 | -2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 3.3 | 1.2 | 7.6 | 8.7 | 10.4 |
Mitochondria | 2.9 | 0.9 | 5.6 | 6.6 | 3.9 | 0 | 0 | 0 | 0 | 9.6 | 1.9 | 3.4 | -3.3 | -3.5 | -5.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.5 | 0.5 | 0.7 | 0 | -0.7 | 0 | 0 | 0 | 0 | -2.8 | -1.9 | -1.5 | -3.1 | -3.1 | -3.4 |
SpindlePole | -3.9 | -2.1 | -3.0 | -2.7 | -1.6 | 0 | 0 | 0 | 0 | -6.9 | -5.9 | -6.2 | -4.5 | -4.1 | -3.8 |
Vacuole | -3.2 | -1.5 | -2.2 | -3.1 | -0.4 | -4.5 | -3.0 | -2.5 | -4.0 | -2.1 | -0.2 | -1.9 | -0.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9054 | 1.7417 | 1.3572 | 0.8529 | 0.9032 | 1.4995 | 1.0768 | 2.1609 | 1.7614 | 1.5498 | 1.6152 | 1.8527 | 1.208 | 1.9782 | 1.4216 | 1.7029 | 1.5282 | 1.4198 |
Actin | 0.031 | 0.0083 | 0.0094 | 0.0039 | 0.0071 | 0.0253 | 0.0105 | 0.0101 | 0.0257 | 0.0051 | 0.0445 | 0.0085 | 0.0081 | 0.0054 | 0.0057 | 0.006 | 0.0025 | 0.0042 |
Bud | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0011 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0014 | 0.0008 | 0.0004 | 0.0006 | 0.0009 | 0.0007 | 0.001 | 0.0003 | 0.0006 | 0.0004 | 0.0012 | 0.0005 | 0.0004 | 0.0015 | 0.0007 | 0.0008 | 0.0004 | 0.0004 |
Bud Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0023 | 0.0028 | 0.0005 | 0.0033 | 0.0023 | 0.0007 | 0.0019 | 0.0007 | 0.0006 | 0.0003 | 0.0026 | 0.0003 | 0.0005 | 0.0026 | 0.0012 | 0.0012 | 0.0006 | 0.0002 |
Cell Periphery | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.016 | 0.0137 | 0.0054 | 0.0028 | 0.0089 | 0.0025 | 0.0073 | 0.0062 | 0.0035 | 0.0035 | 0.002 | 0.0021 | 0.0026 | 0.0156 | 0.0041 | 0.0105 | 0.0034 | 0.0018 |
Cytoplasmic Foci | 0.2169 | 0.2305 | 0.2141 | 0.2313 | 0.2056 | 0.1722 | 0.2189 | 0.2638 | 0.2177 | 0.1691 | 0.1985 | 0.2024 | 0.2264 | 0.2323 | 0.2239 | 0.2126 | 0.2161 | 0.216 |
Eisosomes | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0016 | 0.0009 | 0.0006 | 0.0012 | 0.0014 | 0.0011 | 0.0018 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0009 | 0.0003 |
Endosome | 0.3066 | 0.3319 | 0.3557 | 0.3982 | 0.2627 | 0.2564 | 0.3153 | 0.286 | 0.2967 | 0.3978 | 0.2949 | 0.2228 | 0.316 | 0.2398 | 0.3196 | 0.3367 | 0.2926 | 0.2266 |
Golgi | 0.2167 | 0.2918 | 0.2689 | 0.2426 | 0.3258 | 0.3496 | 0.3035 | 0.3254 | 0.3479 | 0.2976 | 0.3618 | 0.4297 | 0.2842 | 0.3019 | 0.3081 | 0.3175 | 0.2863 | 0.3612 |
Lipid Particles | 0.0835 | 0.0279 | 0.0277 | 0.0166 | 0.0721 | 0.031 | 0.0284 | 0.0042 | 0.0124 | 0.0164 | 0.0095 | 0.0104 | 0.0242 | 0.007 | 0.0158 | 0.0241 | 0.023 | 0.0206 |
Mitochondria | 0.0145 | 0.0145 | 0.0187 | 0.0216 | 0.0206 | 0.0163 | 0.0106 | 0.0049 | 0.0146 | 0.0051 | 0.0162 | 0.0108 | 0.0188 | 0.0051 | 0.0165 | 0.0098 | 0.0297 | 0.0109 |
None | 0.0047 | 0.0046 | 0.0011 | 0.0006 | 0.0002 | 0.0003 | 0.008 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0039 | 0.035 | 0.0026 | 0.0006 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0055 | 0.0002 | 0.0015 | 0.0013 | 0.0007 | 0.0014 | 0.005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Nucleolus | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0049 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0046 | 0.0001 | 0.0004 | 0.0007 | 0.0005 | 0.0003 | 0.0022 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0864 | 0.0627 | 0.0882 | 0.0686 | 0.0841 | 0.1368 | 0.0809 | 0.0936 | 0.0765 | 0.1003 | 0.0654 | 0.1088 | 0.1053 | 0.1485 | 0.0979 | 0.0757 | 0.14 | 0.1546 |
Punctate Nuclear | 0.0032 | 0.0038 | 0.0029 | 0.0007 | 0.0005 | 0.0009 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0003 | 0.0005 | 0.0012 | 0.0006 |
Vacuole | 0.0025 | 0.0032 | 0.0021 | 0.0023 | 0.0026 | 0.002 | 0.0019 | 0.0029 | 0.0013 | 0.0018 | 0.0006 | 0.0008 | 0.0015 | 0.0018 | 0.0015 | 0.0016 | 0.0012 | 0.0013 |
Vacuole Periphery | 0.0013 | 0.0013 | 0.0016 | 0.0019 | 0.0019 | 0.0015 | 0.0013 | 0.0007 | 0.001 | 0.0014 | 0.0008 | 0.0008 | 0.0008 | 0.0006 | 0.001 | 0.0011 | 0.0009 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.2213 | 17.647 | 15.5071 | 20.8623 | 21.2167 | 22.8451 | 20.4254 | 23.1348 | 23.656 | 23.9732 |
Translational Efficiency | 1.2839 | 0.8112 | 0.9254 | 0.787 | 0.7732 | 1.0067 | 0.6219 | 0.6585 | 0.7351 | 0.7266 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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