Standard name
Human Ortholog
Description Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0.18 0.08 0.19 0 0 0 0.05 0.06 0.06
Bud 0 0 0.05 0.05 0.1 0.09 0.1 0.08 0.1 0.12 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.12 0.19 0.14 0.14 0.17 0.19 0.19 0.2 0.21 0.2 0.08 0.16 0.2 0.17 0.19 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.05 0.06 0 0.05 0 0 0 0 0 0
Endosome 0.19 0.14 0.28 0.24 0.3 0.2 0.15 0.15 0 0 0 0.08 0 0.23 0.19 0.28 0.28 0.17 0.33 0.34 0.37 0.36 0.35 0.25
Golgi 0.09 0.17 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.06 0.33 0.39 0.48 0.32 0.33 0.19 0.29 0.32 0.37
Mitochondria 0.68 0.66 0.31 0.44 0.35 0.55 0.57 0.48 0.75 0.76 0.74 0.63 0.82 0.42 0.5 0.15 0.14 0.09 0.09 0.12 0.19 0.15 0.11 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0.08 0.07 0.1 0.1 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0.16 0.06 0.12 0.06 0.11 0.12
SpindlePole 0.07 0 0.3 0.16 0.22 0.19 0.21 0.24 0.1 0.11 0.1 0.17 0 0 0 0.08 0.1 0.13 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0.05 0.08 0 0.05 0 0 0.07 0 0 0 0 0 0.08 0 0.06 0.08 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 14 15 16 6 4 7 4 2 5 1 3 5 4 0 19 9 26 10 11 7 4 11 10
Bud 13 4 12 13 25 31 45 24 35 42 39 32 4 1 4 1 1 4 1 1 1 0 0 0
Bud Neck 0 0 0 1 1 1 2 2 1 1 0 2 0 0 0 0 0 0 0 2 1 0 0 0
Bud Site 0 0 0 1 0 1 4 4 10 18 10 12 0 0 0 0 0 0
Cell Periphery 0 1 2 0 2 2 3 3 2 3 2 7 1 1 0 0 0 2 0 0 0 0 0 0
Cytoplasm 9 28 28 48 36 45 80 54 67 70 67 79 11 17 25 18 21 14 1 6 3 0 0 1
Endoplasmic Reticulum 9 11 0 3 0 3 0 0 3 2 0 1 13 7 6 6 2 7 1 0 1 0 2 4
Endosome 58 39 68 61 78 66 69 42 15 8 13 31 2 24 24 30 31 23 112 117 124 28 70 44
Golgi 26 46 9 4 2 0 0 0 0 0 0 0 9 14 8 36 43 65 110 115 62 23 64 63
Mitochondria 202 180 76 113 92 182 269 139 258 271 241 250 117 44 62 16 15 12 30 40 64 11 23 21
Nucleus 0 1 0 2 2 1 1 4 3 3 4 5 0 2 1 0 0 1 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 2 1 0 0 2 0 0 0 1 0 1 0 1 0
Nucleolus 8 1 0 0 1 2 1 9 0 4 4 2 2 0 2 4 1 1 0 0 0 0 0 0
Peroxisomes 17 8 20 18 25 33 39 19 9 1 7 11 2 3 5 0 0 0 53 20 40 4 23 21
SpindlePole 20 12 73 40 58 64 99 69 35 41 33 68 2 2 3 9 11 17 10 8 13 4 1 1
Vac/Vac Membrane 11 14 20 5 13 13 14 21 3 10 11 7 4 8 4 7 9 11 6 15 6 0 1 1
Unique Cell Count 298 273 242 257 262 331 469 289 345 358 324 396 142 105 125 108 109 135 344 346 335 79 204 174
Labelled Cell Count 390 359 323 325 341 448 633 394 443 479 434 511 172 127 146 146 143 183 344 346 335 79 204 174


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.4 6.2 6.1 6.5 5.0 5.0 5.6 4.5 4.2 4.2 4.7 5.8 6.2 6.1 10.0 10.4 9.5 5.6 5.7 6.5
Std Deviation (1e-4) 0.7 1.1 1.3 1.8 1.6 1.4 1.0 1.6 1.8 1.2 1.6 1.0 0.8 1.1 0.9 1.7 2.0 1.7 0.8 1.3 1.9
Intensity Change (Log2) -0.04 0.05 -0.32 -0.33 -0.17 -0.48 -0.57 -0.57 -0.41 -0.11 -0.01 -0.03 0.67 0.74 0.61 -0.15 -0.13 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.2 -3.3 -3.4 -3.0 0 0 0 0 -1.1 -0.9 -2.8 3.3 0.7 3.9
Bud 0.1 2.0 2.0 2.2 1.5 0 0 0 0 -1.0 0 -0.8 0 0 -0.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 0.7 0.7 1.9 2.3 2.5 2.6 2.9 2.7 -1.2 1.2 2.2 1.3 1.9 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.1 0.4 -2.3 -4.3 -3.8 0 0 0 0 -6.5 -1.0 -1.9 -0.1 0.1 -2.4
Golgi -1.5 -2.3 0 0 0 0 0 0 0 1.2 3.3 1.2 7.6 8.7 10.4
Mitochondria 2.9 0.9 5.6 6.6 3.9 0 0 0 0 9.6 1.9 3.4 -3.3 -3.5 -5.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.5 0.5 0.7 0 -0.7 0 0 0 0 -2.8 -1.9 -1.5 -3.1 -3.1 -3.4
SpindlePole -3.9 -2.1 -3.0 -2.7 -1.6 0 0 0 0 -6.9 -5.9 -6.2 -4.5 -4.1 -3.8
Vacuole -3.2 -1.5 -2.2 -3.1 -0.4 -4.5 -3.0 -2.5 -4.0 -2.1 -0.2 -1.9 -0.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9054 1.7417 1.3572 0.8529 0.9032 1.4995 1.0768 2.1609 1.7614 1.5498 1.6152 1.8527 1.208 1.9782 1.4216 1.7029 1.5282 1.4198
Actin 0.031 0.0083 0.0094 0.0039 0.0071 0.0253 0.0105 0.0101 0.0257 0.0051 0.0445 0.0085 0.0081 0.0054 0.0057 0.006 0.0025 0.0042
Bud 0.0003 0.0004 0.0001 0.0007 0.0011 0.0004 0.0002 0.0001 0.0002 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001
Bud Neck 0.0014 0.0008 0.0004 0.0006 0.0009 0.0007 0.001 0.0003 0.0006 0.0004 0.0012 0.0005 0.0004 0.0015 0.0007 0.0008 0.0004 0.0004
Bud Periphery 0.0002 0.0002 0.0001 0.0008 0.0007 0.0004 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0023 0.0028 0.0005 0.0033 0.0023 0.0007 0.0019 0.0007 0.0006 0.0003 0.0026 0.0003 0.0005 0.0026 0.0012 0.0012 0.0006 0.0002
Cell Periphery 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0001 0 0.0001 0.0002 0.0001 0 0 0
Cytoplasm 0.016 0.0137 0.0054 0.0028 0.0089 0.0025 0.0073 0.0062 0.0035 0.0035 0.002 0.0021 0.0026 0.0156 0.0041 0.0105 0.0034 0.0018
Cytoplasmic Foci 0.2169 0.2305 0.2141 0.2313 0.2056 0.1722 0.2189 0.2638 0.2177 0.1691 0.1985 0.2024 0.2264 0.2323 0.2239 0.2126 0.2161 0.216
Eisosomes 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0002 0 0 0 0
Endoplasmic Reticulum 0.0016 0.0009 0.0006 0.0012 0.0014 0.0011 0.0018 0.0002 0.0003 0.0003 0.0003 0.0004 0.0005 0.0004 0.0004 0.0004 0.0009 0.0003
Endosome 0.3066 0.3319 0.3557 0.3982 0.2627 0.2564 0.3153 0.286 0.2967 0.3978 0.2949 0.2228 0.316 0.2398 0.3196 0.3367 0.2926 0.2266
Golgi 0.2167 0.2918 0.2689 0.2426 0.3258 0.3496 0.3035 0.3254 0.3479 0.2976 0.3618 0.4297 0.2842 0.3019 0.3081 0.3175 0.2863 0.3612
Lipid Particles 0.0835 0.0279 0.0277 0.0166 0.0721 0.031 0.0284 0.0042 0.0124 0.0164 0.0095 0.0104 0.0242 0.007 0.0158 0.0241 0.023 0.0206
Mitochondria 0.0145 0.0145 0.0187 0.0216 0.0206 0.0163 0.0106 0.0049 0.0146 0.0051 0.0162 0.0108 0.0188 0.0051 0.0165 0.0098 0.0297 0.0109
None 0.0047 0.0046 0.0011 0.0006 0.0002 0.0003 0.008 0.0002 0.0003 0.0001 0.0001 0.0003 0.0039 0.035 0.0026 0.0006 0.0002 0.0001
Nuclear Periphery 0.0055 0.0002 0.0015 0.0013 0.0007 0.0014 0.005 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001
Nucleolus 0.0004 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0049 0.0003 0.0001 0.0001 0.0001 0
Nucleus 0.0046 0.0001 0.0004 0.0007 0.0005 0.0003 0.0022 0.0001 0.0001 0 0 0.0002 0.0004 0.0005 0.0001 0.0001 0.0001 0.0001
Peroxisomes 0.0864 0.0627 0.0882 0.0686 0.0841 0.1368 0.0809 0.0936 0.0765 0.1003 0.0654 0.1088 0.1053 0.1485 0.0979 0.0757 0.14 0.1546
Punctate Nuclear 0.0032 0.0038 0.0029 0.0007 0.0005 0.0009 0.0008 0.0003 0.0003 0.0003 0.0003 0.0005 0.0008 0.0009 0.0003 0.0005 0.0012 0.0006
Vacuole 0.0025 0.0032 0.0021 0.0023 0.0026 0.002 0.0019 0.0029 0.0013 0.0018 0.0006 0.0008 0.0015 0.0018 0.0015 0.0016 0.0012 0.0013
Vacuole Periphery 0.0013 0.0013 0.0016 0.0019 0.0019 0.0015 0.0013 0.0007 0.001 0.0014 0.0008 0.0008 0.0008 0.0006 0.001 0.0011 0.0009 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.2213 17.647 15.5071 20.8623 21.2167 22.8451 20.4254 23.1348 23.656 23.9732
Translational Efficiency 1.2839 0.8112 0.9254 0.787 0.7732 1.0067 0.6219 0.6585 0.7351 0.7266

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Sgm1

Sgm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available