Standard name | |
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Human Ortholog | |
Description | Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud | 0 | 0 | 6 | 1 | 7 | 2 | 5 | 14 | 10 | – | 10 | 9 | 2 | 5 | 14 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 9 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 4 | 5 | 13 | 6 | 3 | 9 | 7 | – | 4 | 6 | 6 | 4 | 9 | 12 | 10 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 190 | 269 | 231 | 258 | 543 | 327 | 654 | 782 | 371 | – | 326 | 431 | 327 | 476 | 585 | 437 | 270 | 311 | 189 | 255 | 216 | 249 | 432 | 516 |
Endoplasmic Reticulum | 3 | 1 | 1 | 0 | 3 | 2 | 2 | 1 | 2 | – | 0 | 0 | 0 | 0 | 1 | 14 | 6 | 11 | 1 | 2 | 3 | 4 | 4 | 9 |
Endosome | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 9 | 0 | – | 3 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 0 | 1 | 0 | 0 | 4 | 5 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 1 | 3 | 7 | 19 | 29 | 55 | 158 | 185 | 198 | – | 221 | 284 | 1 | 5 | 20 | 16 | 2 | 4 | 1 | 0 | 8 | 4 | 4 | 7 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 3 | 3 | – | 2 | 0 | 1 | 1 | 0 | 6 | 8 | 6 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | – | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 0 | 2 | 0 | 0 | 7 | 0 | 5 | 9 | 1 | – | 6 | 7 | 3 | 1 | 0 | 11 | 7 | 8 | 0 | 2 | 5 | 5 | 12 | 4 |
Unique Cell Count | 194 | 273 | 236 | 261 | 566 | 343 | 714 | 878 | 454 | 454 | 571 | 333 | 481 | 593 | 456 | 285 | 321 | 197 | 269 | 240 | 274 | 480 | 570 | |
Labelled Cell Count | 201 | 280 | 249 | 285 | 606 | 394 | 832 | 1018 | 597 | 576 | 742 | 341 | 492 | 631 | 501 | 309 | 357 | 197 | 269 | 240 | 274 | 480 | 570 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 7.1 | 5.4 | 5.2 | 5.4 | 4.8 | 4.3 | 4.4 | 3.6 | – | 3.5 | 3.1 | 6.8 | 6.6 | 5.7 | 7.0 | 7.6 | 8.1 | 6.5 | 5.9 | 6.6 |
Std Deviation (1e-4) | 2.2 | 2.1 | 1.3 | 1.4 | 1.3 | 1.3 | 1.4 | 1.3 | 1.2 | – | 1.1 | 0.8 | 1.9 | 1.8 | 1.5 | 1.9 | 1.9 | 1.9 | 1.7 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.02 | -0.16 | -0.33 | -0.3 | -0.59 | – | -0.62 | -0.77 | 0.34 | 0.31 | 0.08 | 0.38 | 0.5 | 0.6 | 0.28 | 0.14 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.8 | 1.3 | 1.8 |
Cytoplasm | 0.9 | -1.4 | -1.6 | -3.3 | -4.2 | -6.0 | – | -8.2 | -7.5 | 0.3 | 1.2 | 0.8 | -1.4 | -1.9 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 2.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.2 | 1.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.3 | 0.4 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.2154 | 29.752 | 21.2456 | 20.966 | 23.0265 | 26.853 | 24.083 | 36.8279 | 31.6705 | 34.133 | 32.794 | 35.201 | 10.7066 | 14.888 | 15.1093 | 15.0325 | 17.3891 | 15.5383 |
Actin | 0.0413 | 0.0009 | 0.0218 | 0.0056 | 0.0003 | 0.0019 | 0.0079 | 0.0002 | 0.0013 | 0.0024 | 0.0059 | 0.0035 | 0.0364 | 0.0097 | 0.0037 | 0.0043 | 0.0798 | 0.008 |
Bud | 0.0012 | 0.0012 | 0.0028 | 0.0059 | 0.0009 | 0.0022 | 0.0011 | 0.0004 | 0.0007 | 0.0036 | 0.001 | 0.0007 | 0.0019 | 0.0021 | 0.0038 | 0.0041 | 0.0051 | 0.0007 |
Bud Neck | 0.0023 | 0.0008 | 0.0011 | 0.0035 | 0.0011 | 0.005 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0007 | 0.0007 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0009 |
Bud Periphery | 0.0012 | 0.0004 | 0.0033 | 0.0036 | 0.0003 | 0.0052 | 0.0007 | 0.0001 | 0.0002 | 0.0013 | 0.0017 | 0.0004 | 0.0015 | 0.0005 | 0.0013 | 0.0025 | 0.0034 | 0.0006 |
Bud Site | 0.0078 | 0.005 | 0.0068 | 0.0581 | 0.0001 | 0.0004 | 0.0015 | 0.0004 | 0.0004 | 0.0003 | 0.0006 | 0.0001 | 0.0037 | 0.0052 | 0.0044 | 0.0023 | 0.0024 | 0.0003 |
Cell Periphery | 0.0025 | 0.0014 | 0.0033 | 0.0017 | 0.0002 | 0.0015 | 0.0007 | 0.0021 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0002 |
Cytoplasm | 0.6536 | 0.9623 | 0.7989 | 0.8044 | 0.83 | 0.8482 | 0.8217 | 0.9836 | 0.9342 | 0.9603 | 0.8824 | 0.9291 | 0.7271 | 0.9465 | 0.9095 | 0.8694 | 0.812 | 0.9066 |
Cytoplasmic Foci | 0.019 | 0.0022 | 0.0105 | 0.0245 | 0.0062 | 0.005 | 0.0085 | 0.002 | 0.0067 | 0.0041 | 0.0032 | 0.0059 | 0.0333 | 0.0078 | 0.0107 | 0.0221 | 0.0129 | 0.012 |
Eisosomes | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.1139 | 0.0042 | 0.027 | 0.0057 | 0.0662 | 0.039 | 0.0264 | 0.0014 | 0.004 | 0.0015 | 0.0109 | 0.0138 | 0.0748 | 0.0097 | 0.0137 | 0.007 | 0.0377 | 0.0248 |
Endosome | 0.0412 | 0.0023 | 0.017 | 0.0153 | 0.0306 | 0.0134 | 0.0135 | 0.0015 | 0.0158 | 0.0097 | 0.0134 | 0.0153 | 0.0376 | 0.0075 | 0.0218 | 0.0379 | 0.0214 | 0.0226 |
Golgi | 0.0104 | 0.0004 | 0.0046 | 0.0017 | 0.0018 | 0.0018 | 0.003 | 0.0001 | 0.0064 | 0.0003 | 0.0022 | 0.0042 | 0.0107 | 0.0014 | 0.002 | 0.0065 | 0.0015 | 0.0079 |
Lipid Particles | 0.0039 | 0.0001 | 0.0027 | 0.0012 | 0.0001 | 0.0002 | 0.0025 | 0.0006 | 0.0028 | 0 | 0.0002 | 0.0002 | 0.0098 | 0.0004 | 0.0012 | 0.0029 | 0.0001 | 0.0005 |
Mitochondria | 0.0093 | 0.0002 | 0.0212 | 0.0021 | 0.0047 | 0.0025 | 0.0366 | 0.0001 | 0.0084 | 0.0002 | 0.0519 | 0.0103 | 0.0048 | 0.0003 | 0.0042 | 0.0012 | 0.0065 | 0.0043 |
None | 0.0091 | 0.0008 | 0.019 | 0.0028 | 0.0005 | 0.0004 | 0.0062 | 0.0006 | 0.0007 | 0.0007 | 0.0005 | 0.0005 | 0.0146 | 0.001 | 0.0041 | 0.0014 | 0.0019 | 0.0017 |
Nuclear Periphery | 0.0155 | 0.0011 | 0.0173 | 0.0049 | 0.0092 | 0.0362 | 0.04 | 0.0005 | 0.002 | 0.0016 | 0.0054 | 0.0036 | 0.0113 | 0.001 | 0.0027 | 0.003 | 0.0024 | 0.0013 |
Nucleolus | 0.001 | 0.0002 | 0.0003 | 0.0011 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0091 | 0.0017 | 0.0031 | 0.016 | 0.0038 | 0.0037 | 0.0078 | 0.0008 | 0.0012 | 0.0013 | 0.0019 | 0.0017 | 0.003 | 0.001 | 0.0016 | 0.0021 | 0.0014 | 0.0009 |
Peroxisomes | 0.0042 | 0 | 0.0007 | 0.0006 | 0 | 0.0001 | 0.0008 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.008 | 0.0002 | 0.001 | 0.0034 | 0.0005 | 0.0005 |
Punctate Nuclear | 0.0019 | 0.0001 | 0.001 | 0.0017 | 0.0002 | 0.0001 | 0.0015 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0051 | 0.0002 | 0.0006 | 0.0005 | 0.0002 | 0.0002 |
Vacuole | 0.0443 | 0.013 | 0.0273 | 0.0334 | 0.0342 | 0.022 | 0.0134 | 0.005 | 0.0112 | 0.009 | 0.0133 | 0.0071 | 0.0119 | 0.0042 | 0.0108 | 0.0208 | 0.0064 | 0.0046 |
Vacuole Periphery | 0.0069 | 0.0018 | 0.0098 | 0.006 | 0.0095 | 0.0111 | 0.0051 | 0.0004 | 0.003 | 0.0031 | 0.0039 | 0.0026 | 0.0025 | 0.0005 | 0.0019 | 0.0076 | 0.003 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.1048 | 30.7602 | 34.6608 | 46.6459 | 31.7253 | 30.06 | 26.4363 | 27.1334 | 34.2114 | 34.2841 |
Translational Efficiency | 1.1127 | 1.1419 | 0.827 | 0.7992 | 0.9734 | 1.2475 | 0.7035 | 0.4923 | 0.5942 | 0.7034 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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