Standard name | |
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Human Ortholog | |
Description | Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 2 | 5 | 5 | 9 | 12 | 7 | 10 | 3 | 9 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 1 | 4 | 7 | 7 | 13 | 10 | 22 | 19 | 19 | 10 | 29 | 0 | 0 | 1 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 16 | 9 | 8 | 24 | 21 | 30 | 37 | 14 | 22 | 16 | 25 | 7 | 21 | 22 | 52 | 33 | 52 | 1 | 4 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 2 | 3 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 14 | 16 | 19 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 3 | 0 | 2 | 1 | 6 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 14 | 13 | 9 | 2 | 7 | 2 | 1 | 4 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | 128 | 82 | 98 | 106 | 156 | 189 | 272 | 248 | 270 | 181 | 264 | 8 | 4 | 30 | 35 | 17 | 53 | 1 | 12 | 5 | 0 | 4 | 2 |
Nucleus | 151 | 355 | 163 | 158 | 227 | 152 | 196 | 248 | 191 | 215 | 118 | 164 | 66 | 86 | 89 | 524 | 403 | 476 | 136 | 360 | 152 | 37 | 111 | 71 |
Nuclear Periphery | 2 | 0 | 0 | 2 | 2 | 1 | 7 | 5 | 4 | 3 | 2 | 4 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 8 | 5 | 4 | 5 | 8 | 6 | 5 | 12 | 5 | 19 | 4 | 6 | 2 | 5 | 8 | 3 | 2 | 5 | 15 | 13 | 15 | 3 | 16 | 14 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 6 | 4 | 8 | 8 | 3 | 11 | 8 | 2 | 6 | 5 | 7 | 0 | 1 | 1 | 12 | 15 | 14 | 1 | 4 | 3 | 0 | 5 | 7 |
Vac/Vac Membrane | 5 | 11 | 1 | 2 | 5 | 4 | 7 | 3 | 3 | 1 | 2 | 3 | 3 | 3 | 5 | 18 | 19 | 16 | 8 | 23 | 23 | 3 | 23 | 17 |
Unique Cell Count | 168 | 422 | 199 | 197 | 312 | 235 | 320 | 397 | 298 | 361 | 221 | 311 | 80 | 113 | 131 | 619 | 469 | 572 | 175 | 444 | 214 | 51 | 174 | 133 |
Labelled Cell Count | 185 | 524 | 273 | 293 | 398 | 363 | 465 | 621 | 494 | 566 | 342 | 512 | 86 | 122 | 157 | 679 | 522 | 649 | 175 | 444 | 214 | 51 | 174 | 133 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 4.3 | 4.1 | 3.9 | 4.4 | 3.5 | 3.8 | 3.5 | 3.0 | 3.2 | 3.1 | 2.9 | 4.8 | 5.2 | 4.3 | 6.5 | 6.9 | 7.1 | 5.2 | 5.4 | 6.2 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.9 | 1.8 | 2.5 | 1.5 | 2.3 | 1.6 | 1.1 | 1.7 | 1.6 | 1.7 | 0.9 | 1.4 | 1.0 | 1.3 | 1.5 | 1.8 | 1.3 | 1.4 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.09 | -0.22 | -0.12 | -0.24 | -0.46 | -0.36 | -0.39 | -0.52 | 0.23 | 0.33 | 0.06 | 0.67 | 0.75 | 0.78 | 0.33 | 0.41 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.5 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6509 | 0.9441 | 0.6784 | 0.3284 | 1.2774 | 0.5997 | 1.4774 | 2.1256 | 2.0181 | 1.7773 | 1.9339 | 1.7854 | 3.461 | 3.2451 | 3.0296 | 2.6944 | 2.4378 | 2.9256 |
Actin | 0.0153 | 0.0001 | 0.0206 | 0.0266 | 0 | 0.0013 | 0.0012 | 0 | 0.0028 | 0.017 | 0.005 | 0.0036 | 0.0283 | 0.0002 | 0.0043 | 0.0375 | 0.0001 | 0 |
Bud | 0.0005 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0013 | 0 | 0 |
Bud Neck | 0.0098 | 0.0007 | 0.0009 | 0.0021 | 0.0003 | 0.0021 | 0.0036 | 0.0003 | 0.0007 | 0.0006 | 0.0007 | 0.0061 | 0.001 | 0.0002 | 0.0005 | 0.0031 | 0.0007 | 0.0028 |
Bud Periphery | 0.0014 | 0.0002 | 0.0013 | 0.0003 | 0 | 0.0001 | 0.0018 | 0 | 0.0014 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0008 | 0.0045 | 0 | 0.0001 |
Bud Site | 0.0046 | 0.0011 | 0.0078 | 0.0035 | 0 | 0.0001 | 0.0015 | 0.0001 | 0.0025 | 0.001 | 0.0004 | 0.0001 | 0.0014 | 0.0002 | 0.0003 | 0.0021 | 0.0001 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0006 | 0 | 0 |
Cytoplasm | 0.0063 | 0.006 | 0.0026 | 0.0089 | 0.0003 | 0.0008 | 0.0037 | 0.0008 | 0.0042 | 0.0007 | 0.0004 | 0.0051 | 0.0011 | 0.0009 | 0.0021 | 0.0007 | 0.0016 | 0.0007 |
Cytoplasmic Foci | 0.0044 | 0.0004 | 0.0012 | 0.0336 | 0 | 0.0007 | 0.0022 | 0 | 0.0077 | 0.0026 | 0.0251 | 0.0008 | 0.0131 | 0 | 0.0025 | 0.0088 | 0.0003 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.003 | 0.0005 | 0.0005 | 0.0007 | 0 | 0.0002 | 0.001 | 0 | 0.0011 | 0.0004 | 0.0002 | 0.0005 | 0.0009 | 0 | 0.0008 | 0.0011 | 0.0001 | 0 |
Endosome | 0.0102 | 0.0009 | 0.004 | 0.0142 | 0 | 0.0018 | 0.0092 | 0 | 0.0165 | 0.0071 | 0.0219 | 0.0014 | 0.0153 | 0.0001 | 0.0032 | 0.0055 | 0.0005 | 0.0001 |
Golgi | 0.0089 | 0 | 0.0023 | 0.009 | 0 | 0.0004 | 0.0008 | 0 | 0.0037 | 0.0038 | 0.0227 | 0.0007 | 0.0073 | 0 | 0.0014 | 0.0069 | 0 | 0 |
Lipid Particles | 0.0057 | 0.0002 | 0.0023 | 0.022 | 0 | 0.0045 | 0.0018 | 0 | 0.0032 | 0.0092 | 0.033 | 0.0002 | 0.0122 | 0 | 0.0015 | 0.0157 | 0.0001 | 0 |
Mitochondria | 0.0046 | 0.0006 | 0.0159 | 0.0014 | 0.0001 | 0.0012 | 0.006 | 0.0001 | 0.0188 | 0.0045 | 0.0067 | 0.003 | 0.0049 | 0.0003 | 0.0026 | 0.0376 | 0.0002 | 0.0002 |
None | 0.0133 | 0.0246 | 0.0047 | 0.0297 | 0.0001 | 0.014 | 0.0132 | 0.0004 | 0.0066 | 0.0055 | 0.0002 | 0.0038 | 0.0013 | 0.0092 | 0.0154 | 0.0005 | 0.0017 | 0.0019 |
Nuclear Periphery | 0.0128 | 0.008 | 0.0054 | 0.0058 | 0.0012 | 0.004 | 0.012 | 0.0023 | 0.0036 | 0.0029 | 0.0066 | 0.0022 | 0.004 | 0.0039 | 0.01 | 0.0039 | 0.0039 | 0.0009 |
Nucleolus | 0.0273 | 0.0219 | 0.0241 | 0.0221 | 0.0183 | 0.039 | 0.0265 | 0.0157 | 0.0224 | 0.0109 | 0.0329 | 0.0352 | 0.0116 | 0.0238 | 0.0191 | 0.0176 | 0.0391 | 0.0361 |
Nucleus | 0.8458 | 0.9074 | 0.8773 | 0.7332 | 0.9781 | 0.9134 | 0.8888 | 0.9682 | 0.8691 | 0.8961 | 0.7742 | 0.9153 | 0.8741 | 0.9576 | 0.9013 | 0.8349 | 0.9242 | 0.9459 |
Peroxisomes | 0.0017 | 0 | 0.0075 | 0.0276 | 0 | 0.0012 | 0.0007 | 0 | 0.0068 | 0.0035 | 0.0639 | 0.0001 | 0.0127 | 0 | 0.0117 | 0.0124 | 0 | 0 |
Punctate Nuclear | 0.0198 | 0.0264 | 0.014 | 0.0582 | 0.0013 | 0.0146 | 0.0205 | 0.0119 | 0.0196 | 0.0318 | 0.005 | 0.0209 | 0.0083 | 0.0032 | 0.0151 | 0.0022 | 0.027 | 0.0108 |
Vacuole | 0.0026 | 0.0006 | 0.0044 | 0.0004 | 0.0001 | 0.0003 | 0.0034 | 0.0001 | 0.0063 | 0.001 | 0.0004 | 0.0004 | 0.0011 | 0.0001 | 0.0042 | 0.0015 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0012 | 0.0002 | 0 | 0.0002 | 0.0018 | 0 | 0.0019 | 0.0005 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0028 | 0.0013 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3938 | 10.5676 | 12.4282 | 13.9469 | 14.604 | 14.9358 | 17.3882 | 18.2331 | 14.3473 | 18.9731 |
Translational Efficiency | 0.4989 | 0.6117 | 0.4468 | 0.3439 | 0.3326 | 0.4201 | 0.3849 | 0.2787 | 0.295 | 0.2985 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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