Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0.07 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0.06 0 0 0.07 0.07 0.08 0.05 0.07 0.09 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 – 0.94 0.97 0.95 0.93 0.75 0.88 0.8 0.72 0.77 0.67 0.99 1.0 0.99 1.0 0.99 0.98 0.94 0.91 0.84 0.88 0.93 0.77
Endoplasmic Reticulum 0 – 0 0 0 0 0.14 0.05 0.13 0.1 0.12 0.17 0 0 0 0 0.08 0.06 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 3 1 3 7 4 13 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 2 2 6
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 1 1 2 3 8 9 4 7 7 19 1 0 2 1 2 1 0 0 0 1 1 4
Cytoplasm 119 – 15 74 109 39 86 97 66 76 62 80 91 68 104 90 88 135 122 27 20 78 100 102
Endoplasmic Reticulum 0 – 0 1 4 1 16 6 11 10 10 20 1 0 1 4 7 8 1 0 0 2 0 5
Endosome 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 1 1 3 6 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 1 0 5 2 6 1 0 0 0 0 0 0 1 0 0 0 0 2
Nucleus 0 – 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 – 0 0 0 0 0 0 0 5 0 3 0 0 0 0 0 2 0 0 0 0 0 5
Unique Cell Count 120 16 76 115 42 114 110 83 105 81 120 92 68 105 90 89 138 130 30 24 89 109 132
Labelled Cell Count 120 16 76 117 43 118 114 93 115 90 140 93 68 107 95 97 146 130 30 24 89 109 132


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 146.1 – 117.7 121.4 93.0 61.7 58.2 57.9 45.5 47.5 44.2 43.6 139.5 145.7 151.6 238.0 305.2 301.0 127.5 122.7 122.1
Std Deviation (1e-4) 36.2 – 37.1 41.8 38.2 25.3 23.8 20.5 19.3 18.1 13.7 12.3 40.2 44.3 37.8 69.9 71.5 89.1 41.7 54.3 60.6
Intensity Change (Log2) – – – 0.04 -0.34 -0.93 -1.02 -1.02 -1.37 -1.31 -1.41 -1.43 0.25 0.31 0.37 1.02 1.37 1.35 0.12 0.06 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.2 -0.1 -1.6 -0.7 -1.4 -1.8 -1.6 -2.2 1.4 2.1 1.5 2.4 1.4 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 173.4114 243.9982 219.4856 152.7077 176.0452 167.5323 191.7582 253.9583 211.6907 211.3036 250.7893 175.7368 154.7422 237.8715 242.9908 189.1348 193.424 211.2025
Actin 0.0061 0.0007 0.0033 0.0076 0.0013 0.0173 0.0068 0.0006 0.0066 0.0009 0.0005 0.0058 0.0489 0.0027 0.0025 0.0004 0.0769 0.0107
Bud 0.0039 0.0023 0.0044 0.0039 0.0054 0.0051 0.0031 0.0017 0.0042 0.002 0.005 0.0137 0.0068 0.0038 0.0088 0.002 0.0117 0.0102
Bud Neck 0.0012 0.0012 0.0013 0.0008 0.0012 0.0061 0.0043 0.001 0.0013 0.0011 0.0019 0.0019 0.0017 0.0027 0.0009 0.0004 0.0014 0.0025
Bud Periphery 0.0058 0.0019 0.004 0.0036 0.0027 0.0044 0.0038 0.0009 0.0036 0.0014 0.0074 0.0077 0.0108 0.0019 0.0029 0.0026 0.0112 0.0203
Bud Site 0.0032 0.0019 0.0019 0.0024 0.0005 0.001 0.0027 0.0015 0.0036 0.0009 0.0006 0.0028 0.0055 0.0047 0.0033 0.0006 0.0028 0.0006
Cell Periphery 0.0976 0.0571 0.0554 0.0464 0.01 0.0455 0.0834 0.0385 0.0477 0.0738 0.049 0.0226 0.0748 0.0452 0.0306 0.0644 0.0271 0.0093
Cytoplasm 0.3522 0.7162 0.627 0.3181 0.5646 0.4007 0.5098 0.8054 0.6033 0.4898 0.5014 0.4197 0.4265 0.7915 0.7049 0.2787 0.2635 0.4174
Cytoplasmic Foci 0.0136 0.0016 0.0088 0.0041 0.0754 0.0063 0.0031 0.0011 0.0052 0.0191 0.0019 0.0036 0.0149 0.0042 0.0031 0.0012 0.0049 0.0057
Eisosomes 0.0021 0.0001 0.0003 0.001 0.0021 0.0005 0.0013 0.0001 0.0004 0.0006 0 0.0049 0.0027 0.0006 0.0013 0.0001 0.0007 0.0003
Endoplasmic Reticulum 0.0294 0.0045 0.007 0.0104 0.0046 0.018 0.0292 0.0034 0.0037 0.002 0.0029 0.0086 0.0194 0.0025 0.0047 0.0006 0.0132 0.0419
Endosome 0.0213 0.0041 0.0144 0.0989 0.0086 0.0572 0.0135 0.0019 0.0121 0.0048 0.0054 0.0269 0.0064 0.0045 0.0034 0.0014 0.032 0.023
Golgi 0.0102 0.0006 0.0033 0.0076 0.0008 0.067 0.0024 0.0004 0.0032 0.0007 0.0002 0.0021 0.0027 0.0129 0.0005 0.0001 0.0196 0.0048
Lipid Particles 0.009 0.0027 0.0024 0.0129 0.0154 0.0025 0.0045 0.0014 0.0027 0.018 0.0017 0.0038 0.0067 0.0015 0.0025 0.0011 0.0036 0.0012
Mitochondria 0.0385 0.0022 0.0101 0.025 0.0542 0.0437 0.0134 0.0049 0.0113 0.0123 0.0009 0.0135 0.0424 0.0024 0.0143 0.0035 0.093 0.073
None 0.0093 0.0019 0.0056 0.0101 0.0151 0.0022 0.0044 0.0006 0.0105 0.0142 0.0005 0.004 0.0508 0.0014 0.0024 0.2001 0.0026 0.0032
Nuclear Periphery 0.0474 0.0066 0.0094 0.0391 0.0114 0.0086 0.0646 0.0083 0.0075 0.0161 0.0375 0.0565 0.0676 0.0145 0.0181 0.021 0.0356 0.0309
Nucleolus 0.023 0.0041 0.0066 0.0407 0.023 0.0021 0.005 0.0016 0.0042 0.026 0.0023 0.04 0.0113 0.0028 0.0079 0.0111 0.0103 0.0048
Nucleus 0.0192 0.0073 0.0089 0.026 0.0115 0.011 0.0249 0.0092 0.0119 0.0189 0.0222 0.114 0.0376 0.0155 0.0216 0.0305 0.0294 0.0222
Peroxisomes 0.0023 0.0002 0.0004 0.001 0.0041 0.0008 0.0003 0.0001 0.0003 0.0011 0.0001 0.0005 0.0012 0.0003 0.0008 0.0002 0.0011 0.0008
Punctate Nuclear 0.0009 0.0001 0.0013 0.0019 0.0016 0.0002 0.0008 0.0001 0.0002 0.0027 0.0001 0.0011 0.0027 0.0001 0.0002 0.0002 0.0004 0.0002
Vacuole 0.2317 0.1623 0.1827 0.2359 0.1262 0.24 0.1714 0.1009 0.2106 0.2287 0.2902 0.1427 0.1109 0.0704 0.1175 0.2116 0.1666 0.1353
Vacuole Periphery 0.072 0.0204 0.0416 0.1026 0.0604 0.06 0.0475 0.0165 0.0461 0.0648 0.0683 0.1035 0.0478 0.0141 0.0481 0.1683 0.1924 0.1817

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3092.6209 2990.5018 3110.2525 3462.5275 3426.3706 2228.0359 2912.9853 2756.3072 2709.009 3010.4452
Translational Efficiency 0.8452 0.8606 0.9183 0.6088 0.8682 1.1317 0.8755 0.8565 0.8723 0.9177

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rps4a

Rps4a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps4a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available