Standard name
Human Ortholog
Description Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.07 0.11 0.11 0.3 0.29 0.17 0.28 0.18 0.2 0.11 0.15 0.19 0.21 0.22 0.14 0.17 0.19 0 0 0.06 0.06 0 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.12 0 0 0 0.06 0.09 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0.31 0.48 0.37 0.67 0.6 0.77 0.52 0 0 0 0 0 0 0 0 0 0 0.06 0.05
Nucleus 0.68 0.57 0.79 0.71 0.48 0.38 0.41 0.39 0.28 0.27 0.27 0.37 0.69 0.64 0.64 0.36 0.29 0.32 0.67 0.68 0.64 0.48 0.39 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0.07 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.08 0.1
Vac/Vac Membrane 0.27 0.43 0.12 0.19 0.19 0.19 0.18 0.13 0.12 0.12 0.08 0.17 0.15 0.2 0.15 0.43 0.45 0.4 0.11 0.11 0.15 0.15 0.18 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud 0 1 0 0 0 0 9 4 3 5 8 4 0 0 0 0 0 0 17 16 5 12 12 23
Bud Neck 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 3 5
Bud Site 0 1 0 0 1 0 3 1 3 5 5 4 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 20 18 19 19 54 50 36 53 34 43 21 26 39 46 60 63 34 64 13 13 13 15 13 22
Endoplasmic Reticulum 0 0 0 0 0 3 1 0 0 0 0 0 1 0 1 24 12 21 0 0 1 1 1 4
Endosome 4 4 2 5 6 3 0 1 0 2 0 0 2 6 11 54 25 40 10 6 3 15 30 43
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 5 2 1 1 8 9
Mitochondria 31 2 0 2 4 53 103 70 124 130 141 89 2 0 0 20 6 5 3 2 6 4 21 21
Nucleus 226 150 142 119 87 66 88 74 51 59 50 64 144 141 178 162 58 109 249 213 139 120 138 125
Nuclear Periphery 2 0 0 0 1 0 0 1 1 2 0 0 0 0 0 2 0 0 0 0 0 2 2 2
Nucleolus 0 1 0 0 1 0 1 0 2 1 1 1 0 0 1 33 12 19 10 9 2 16 14 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 3 7
SpindlePole 2 1 1 1 4 0 0 1 1 4 0 4 2 0 2 21 4 7 15 9 6 13 29 38
Vac/Vac Membrane 89 112 22 32 34 32 39 24 23 25 15 29 31 44 41 191 90 137 40 34 33 36 63 71
Unique Cell Count 331 263 180 168 181 172 216 191 185 215 183 172 210 221 277 449 199 345 375 313 218 249 356 405
Labelled Cell Count 375 291 187 180 192 207 281 229 242 276 241 221 221 237 294 573 243 407 375 313 218 249 356 405


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.7 7.0 6.4 5.5 4.5 4.4 4.9 4.0 4.3 3.9 4.2 7.4 7.2 7.2 9.0 9.6 8.9 7.2 6.7 6.9
Std Deviation (1e-4) 1.5 2.0 1.5 1.2 1.5 1.2 1.1 1.4 0.9 1.1 1.2 1.0 1.6 1.4 1.7 2.6 2.8 2.5 2.2 1.8 2.1
Intensity Change (Log2) – – – -0.13 -0.35 -0.64 -0.68 -0.53 -0.83 -0.7 -0.85 -0.76 0.08 0.04 0.02 0.35 0.44 0.33 0.03 -0.07 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 4.6 4.4 1.8 4.2 2.1 2.6 0.3 1.3 2.2 2.8 3.1 1.2 1.8 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.3 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 1.8 4.3 4.3 4.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 9.0 0 0 0 0 0 0 0 2.9 0 0
Nucleus -1.7 -6.1 -7.7 -7.7 -7.8 -9.8 -10.2 -9.8 -7.9 -2.3 -3.3 -3.3 -9.7 -9.7 -10.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.3 3.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Vacuole 1.8 1.7 1.7 1.6 0.1 0.1 -0.2 -1.3 1.2 0.7 2.1 0.8 7.3 7.0 6.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5244 1.4034 1.5028 2.209 1.8838 1.3267 0.7586 0.5539 0.4859 0.4058 -0.0258 0.4148 0.5026 0.0453 -0.1586 0.0752 0.6304 -0.0897
Actin 0.0164 0.002 0.0018 0.0218 0.0031 0.0083 0.0001 0.0008 0.0004 0.0002 0.0002 0.0004 0.0071 0.0006 0.0007 0.0114 0.0075 0.0004
Bud 0.001 0.0014 0.0031 0.0046 0.0012 0.0028 0.0001 0.0007 0.0002 0.0001 0 0.0002 0.0004 0.0007 0.0025 0.0034 0.0144 0.0009
Bud Neck 0.0053 0.0009 0.0015 0.0048 0.002 0.0101 0.0005 0.001 0.0007 0.0009 0.0008 0.0031 0.0038 0.0007 0.0048 0.0106 0.0019 0.0077
Bud Periphery 0.0017 0.0008 0.0022 0.0091 0.0024 0.0029 0.0001 0.0004 0.0002 0.0001 0 0.0002 0.0003 0.0006 0.002 0.0014 0.0043 0.0006
Bud Site 0.0062 0.0035 0.0039 0.021 0.003 0.0017 0.0003 0.0052 0.0004 0.0002 0.0001 0.0002 0.0057 0.0079 0.0035 0.0013 0.0014 0.0005
Cell Periphery 0.0005 0.0001 0.0001 0.0014 0.0002 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0002 0.0002 0.0001 0
Cytoplasm 0.0846 0.1429 0.083 0.0726 0.1039 0.0646 0.0949 0.1316 0.0685 0.0802 0.0677 0.1054 0.112 0.2163 0.1421 0.061 0.2495 0.1314
Cytoplasmic Foci 0.0342 0.006 0.0135 0.0159 0.0731 0.0075 0.0026 0.004 0.0022 0.0031 0.0015 0.003 0.0473 0.0041 0.0087 0.0067 0.0598 0.0027
Eisosomes 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0 0.0001 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0081 0.0036 0.0022 0.0036 0.0043 0.0019 0.001 0.003 0.0009 0.0014 0.0009 0.001 0.0054 0.0056 0.003 0.0013 0.0067 0.0017
Endosome 0.0661 0.0048 0.0175 0.0325 0.1062 0.012 0.0011 0.0022 0.001 0.0036 0.0012 0.0015 0.0359 0.0077 0.0176 0.0053 0.018 0.0028
Golgi 0.0142 0.0003 0.0048 0.0092 0.0223 0.0034 0 0.0001 0 0.0001 0 0 0.0054 0.0021 0.0027 0.008 0.0132 0.0001
Lipid Particles 0.0299 0.0004 0.0143 0.0107 0.0168 0.0045 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0168 0.0002 0.0042 0.0018 0.0442 0.0001
Mitochondria 0.0062 0.0069 0.0063 0.0279 0.0337 0.0088 0.0003 0.0008 0.0006 0.0008 0.0003 0.0005 0.001 0.0034 0.0085 0.1097 0.0041 0.0004
None 0.1751 0.3065 0.2424 0.2425 0.225 0.1675 0.186 0.2906 0.2139 0.2051 0.1683 0.1679 0.1365 0.1769 0.124 0.1656 0.1752 0.0612
Nuclear Periphery 0.0386 0.0172 0.0188 0.0136 0.0268 0.0153 0.0068 0.0115 0.0101 0.0123 0.0119 0.0074 0.0144 0.0205 0.0136 0.0108 0.0167 0.0094
Nucleolus 0.0126 0.0066 0.0124 0.0145 0.0034 0.011 0.0037 0.0064 0.0051 0.0085 0.008 0.0066 0.0095 0.0023 0.004 0.0037 0.0027 0.0062
Nucleus 0.3866 0.4315 0.5069 0.3678 0.2871 0.5739 0.6563 0.4601 0.6391 0.5655 0.6379 0.6017 0.5686 0.5313 0.6347 0.5348 0.226 0.7313
Peroxisomes 0.0185 0.0007 0.0077 0.0091 0.0199 0.0022 0.0001 0.0005 0.0001 0.0002 0 0.0001 0.007 0.0009 0.0041 0.004 0.044 0.0001
Punctate Nuclear 0.072 0.0611 0.0507 0.0668 0.0437 0.0952 0.0441 0.0789 0.0554 0.1145 0.1006 0.0999 0.016 0.0138 0.0161 0.0566 0.1045 0.0411
Vacuole 0.0157 0.0022 0.0056 0.0405 0.0176 0.004 0.0017 0.0016 0.0006 0.0026 0.0004 0.0006 0.0055 0.0037 0.0021 0.0016 0.0046 0.0012
Vacuole Periphery 0.0062 0.0004 0.0012 0.0098 0.0041 0.0021 0.0001 0.0003 0.0002 0.0005 0.0001 0.0002 0.0015 0.0008 0.0007 0.0007 0.0014 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4168 10.0744 15.6309 15.5554 29.2233 10.2395 16.4369 10.3125 11.908 29.5219
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (89%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hms2

Hms2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hms2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available