Standard name | |
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Human Ortholog | |
Description | Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 1 | 0 | – | – | – | 1 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 8 | 3 | 1 | 5 | 6 |
Bud | 4 | 6 | 0 | – | – | – | 0 | – | 1 | 1 | 1 | 0 | 2 | 1 | 0 | 3 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 1 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 12 | 1 | – | – | – | 2 | – | 3 | 0 | 1 | 0 | 3 | 2 | 6 | 17 | 14 | 7 | 0 | 1 | 0 | 1 | 1 | 0 |
Endoplasmic Reticulum | 1 | 3 | 1 | – | – | – | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 4 | 2 | 0 | 2 | 0 | 0 | 0 | 0 |
Endosome | 38 | 66 | 18 | – | – | – | 34 | – | 11 | 3 | 12 | 7 | 17 | 11 | 32 | 63 | 55 | 43 | 106 | 124 | 41 | 24 | 49 | 46 |
Golgi | 2 | 4 | 0 | – | – | – | 3 | – | 2 | 2 | 2 | 0 | 1 | 1 | 0 | 2 | 10 | 3 | 9 | 11 | 4 | 4 | 10 | 12 |
Mitochondria | 60 | 63 | 5 | – | – | – | 13 | – | 24 | 14 | 22 | 14 | 16 | 3 | 8 | 11 | 16 | 14 | 8 | 19 | 2 | 5 | 4 | 5 |
Nucleus | 1 | 3 | 0 | – | – | – | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | – | – | – | 0 | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 7 | 1 | 0 | 2 | 0 | 0 | 2 | 1 |
Nucleolus | 13 | 25 | 2 | – | – | – | 3 | – | 9 | 4 | 5 | 2 | 8 | 1 | 3 | 51 | 42 | 25 | 0 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 8 | 14 | 3 | – | – | – | 5 | – | 1 | 2 | 0 | 0 | 2 | 3 | 7 | 5 | 6 | 2 | 6 | 8 | 5 | 1 | 8 | 5 |
SpindlePole | 15 | 20 | 3 | – | – | – | 5 | – | 1 | 0 | 3 | 1 | 4 | 6 | 10 | 22 | 22 | 25 | 2 | 5 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 5 | 19 | 0 | – | – | – | 10 | – | 3 | 0 | 3 | 2 | 1 | 2 | 2 | 15 | 13 | 7 | 2 | 23 | 0 | 3 | 3 | 4 |
Unique Cell Count | 119 | 171 | 25 | 48 | 37 | 16 | 38 | 20 | 42 | 23 | 49 | 129 | 124 | 84 | 145 | 219 | 62 | 46 | 89 | 91 | ||||
Labelled Cell Count | 151 | 238 | 34 | 77 | 56 | 26 | 52 | 28 | 55 | 30 | 69 | 197 | 192 | 130 | 145 | 219 | 62 | 46 | 89 | 91 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 6.8 | 6.8 | – | – | – | 10.1 | – | 10.3 | 9.8 | 10.5 | 9.0 | 6.6 | 8.5 | 7.6 | 9.7 | 12.4 | 9.9 | 8.1 | 8.5 | 9.3 |
Std Deviation (1e-4) | 1.6 | 1.8 | 1.5 | – | – | – | 2.5 | – | 2.7 | 2.8 | 3.6 | 2.3 | 1.7 | 1.8 | 1.5 | 2.7 | 3.6 | 3.5 | 2.0 | 2.8 | 2.6 |
Intensity Change (Log2) | – | – | – | – | – | – | 0.57 | – | 0.59 | 0.52 | 0.63 | 0.41 | -0.06 | 0.31 | 0.15 | 0.5 | 0.86 | 0.53 | 0.25 | 0.32 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | – | – | – | -0.1 | – | -3.3 | -3.3 | -3.1 | -2.5 | -2.5 | -1.7 | -0.6 | -2.1 | -2.5 | -1.8 |
Golgi | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | – | – | 0.7 | – | 3.5 | 4.2 | 3.0 | 3.4 | 1.5 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.6 | 2.2 |
Peroxisomes | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 |
Vacuole | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6365 | 2.8202 | 2.6053 | 1.1441 | 1.3952 | 1.9787 | 4.2752 | 7.7452 | 7.7644 | 7.2538 | 6.6451 | 7.2635 | 3.3576 | 6.2174 | 4.7219 | 6.7671 | 3.8455 | 4.0394 |
Actin | 0.0542 | 0.0047 | 0.0209 | 0.0438 | 0.01 | 0.0074 | 0.0005 | 0.0423 | 0.0006 | 0.0001 | 0.0003 | 0.0016 | 0.0326 | 0.0009 | 0.001 | 0.0008 | 0 | 0.0024 |
Bud | 0.0018 | 0.0012 | 0.0003 | 0.0012 | 0.0003 | 0.0027 | 0 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0.0022 | 0.0003 | 0.0011 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0064 | 0.0009 | 0.0013 | 0.0049 | 0.0006 | 0.0005 | 0.0002 | 0.001 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0029 | 0.0006 | 0.0075 | 0 | 0.0006 |
Bud Periphery | 0.0027 | 0.0013 | 0.0004 | 0.0022 | 0.0005 | 0.0017 | 0 | 0.0005 | 0.0003 | 0 | 0 | 0.0001 | 0.0034 | 0.0003 | 0.0026 | 0.0004 | 0 | 0.0003 |
Bud Site | 0.0086 | 0.0032 | 0.0013 | 0.0247 | 0.0008 | 0.0007 | 0.0001 | 0.0024 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.008 | 0.0059 | 0.0018 | 0.0037 | 0 | 0.0004 |
Cell Periphery | 0.0011 | 0.0005 | 0.0006 | 0.0013 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0016 | 0.0006 | 0.0011 | 0.0009 | 0 | 0.0013 |
Cytoplasm | 0.0322 | 0.005 | 0.0025 | 0.0012 | 0.0098 | 0.0181 | 0.0006 | 0.0057 | 0.0011 | 0.0015 | 0.0008 | 0.0005 | 0.0202 | 0.0013 | 0.0042 | 0.0056 | 0.0011 | 0.0013 |
Cytoplasmic Foci | 0.1133 | 0.1318 | 0.155 | 0.0682 | 0.0949 | 0.1378 | 0.1398 | 0.1442 | 0.1406 | 0.1255 | 0.1254 | 0.0821 | 0.0798 | 0.2328 | 0.2073 | 0.1697 | 0.034 | 0.097 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0025 | 0 | 0 | 0.0006 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0083 | 0.0015 | 0.0068 | 0.0007 | 0.0058 | 0.003 | 0.0003 | 0.0009 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0133 | 0.0004 | 0.0046 | 0.0041 | 0.0002 | 0.005 |
Endosome | 0.2125 | 0.3458 | 0.313 | 0.3209 | 0.3256 | 0.4108 | 0.2034 | 0.2688 | 0.3076 | 0.3382 | 0.282 | 0.1658 | 0.1622 | 0.1938 | 0.1702 | 0.3408 | 0.4635 | 0.2408 |
Golgi | 0.0796 | 0.1383 | 0.1181 | 0.0492 | 0.1366 | 0.1039 | 0.1036 | 0.2123 | 0.1099 | 0.0615 | 0.1251 | 0.1011 | 0.0476 | 0.0863 | 0.0678 | 0.1194 | 0.0203 | 0.1515 |
Lipid Particles | 0.2139 | 0.2697 | 0.2423 | 0.2853 | 0.2966 | 0.2045 | 0.4151 | 0.1016 | 0.2493 | 0.4396 | 0.433 | 0.5642 | 0.2065 | 0.2657 | 0.3511 | 0.161 | 0.4584 | 0.3086 |
Mitochondria | 0.0795 | 0.0142 | 0.014 | 0.0626 | 0.0333 | 0.0061 | 0.0256 | 0.0487 | 0.0627 | 0.0012 | 0.0026 | 0.0203 | 0.0455 | 0.0109 | 0.0615 | 0.0042 | 0.0006 | 0.0486 |
None | 0.0252 | 0.0051 | 0.0003 | 0.0002 | 0.0026 | 0.0246 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.3105 | 0.0002 | 0.0007 | 0.0421 | 0 | 0.0007 |
Nuclear Periphery | 0.0329 | 0.0008 | 0.0129 | 0.0004 | 0.0312 | 0.0022 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0095 | 0.0003 | 0.0024 | 0.0028 | 0.0002 | 0.0113 |
Nucleolus | 0.0027 | 0.0021 | 0.0081 | 0.001 | 0.0008 | 0.0003 | 0.0002 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0013 | 0.002 | 0.0009 | 0.0064 | 0 | 0.0013 |
Nucleus | 0.02 | 0.0005 | 0.0011 | 0.0006 | 0.0077 | 0.0013 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0013 | 0.0004 | 0.0007 | 0.002 | 0 | 0.0007 |
Peroxisomes | 0.0434 | 0.0329 | 0.0444 | 0.0356 | 0.0144 | 0.045 | 0.1053 | 0.0517 | 0.1073 | 0.0222 | 0.0202 | 0.0503 | 0.0281 | 0.1705 | 0.0903 | 0.0238 | 0.0103 | 0.0315 |
Punctate Nuclear | 0.0087 | 0.0022 | 0.0013 | 0.0008 | 0.0071 | 0.0014 | 0.0006 | 0.0004 | 0.0011 | 0.0021 | 0.0006 | 0.0007 | 0.0082 | 0.0011 | 0.001 | 0.0172 | 0.0002 | 0.0006 |
Vacuole | 0.0394 | 0.0296 | 0.042 | 0.0878 | 0.0125 | 0.0177 | 0.0024 | 0.1059 | 0.0115 | 0.0042 | 0.0037 | 0.0072 | 0.0108 | 0.0208 | 0.0224 | 0.072 | 0.0083 | 0.0782 |
Vacuole Periphery | 0.0132 | 0.0087 | 0.0135 | 0.0071 | 0.0083 | 0.01 | 0.0017 | 0.0107 | 0.0039 | 0.0027 | 0.005 | 0.0039 | 0.004 | 0.0024 | 0.0069 | 0.0146 | 0.0028 | 0.0179 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.6744 | 33.867 | 28.2721 | 34.8164 | 45.0264 | 44.1749 | 33.5295 | 38.0379 | 47.5023 | 34.5765 |
Translational Efficiency | 1.498 | 1.5968 | 1.5353 | 1.83 | 1.181 | 1.6613 | 1.5543 | 1.5826 | 1.404 | 1.4822 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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