Standard name | |
---|---|
Human Ortholog | |
Description | Phosphatidylinositol:ceramide phosphoinositol transferase; required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; also known as IPC synthase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 39 | 64 | 11 | 6 | 7 | 26 | 12 | 18 | 11 | 17 | 17 | 27 | 28 | 8 | 6 | 33 | 28 | 44 | 9 | 34 | 22 | 17 | 15 | 21 |
Bud | 5 | 3 | 15 | 9 | 15 | 57 | 34 | 29 | 30 | 37 | 31 | 36 | 1 | 2 | 1 | 2 | 3 | 3 | 0 | 4 | 0 | 2 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 3 | 5 | 7 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 3 | 1 | 3 | 11 | 0 | 7 | 6 | 2 | 7 | 9 | 2 | 1 | 1 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 22 | 8 | 14 | 31 | 21 | 19 | 15 | 28 | 20 | 10 | 23 | 9 | 8 | 13 | 11 | 22 | 13 | 1 | 1 | 1 | 2 | 3 | 2 |
Endoplasmic Reticulum | 10 | 25 | 8 | 2 | 13 | 12 | 18 | 8 | 16 | 16 | 19 | 32 | 13 | 11 | 3 | 5 | 9 | 4 | 2 | 2 | 2 | 3 | 2 | 2 |
Endosome | 10 | 7 | 43 | 29 | 32 | 42 | 43 | 43 | 12 | 38 | 7 | 9 | 16 | 18 | 21 | 20 | 17 | 19 | 39 | 45 | 40 | 36 | 29 | 25 |
Golgi | 335 | 335 | 224 | 121 | 125 | 38 | 42 | 74 | 55 | 56 | 28 | 52 | 355 | 356 | 329 | 194 | 172 | 185 | 445 | 399 | 521 | 311 | 344 | 321 |
Mitochondria | 302 | 268 | 506 | 297 | 548 | 684 | 876 | 911 | 641 | 742 | 708 | 899 | 175 | 160 | 106 | 26 | 26 | 27 | 11 | 27 | 23 | 14 | 13 | 11 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 5 | 4 | 1 | 4 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 6 | 5 | 11 | 6 | 6 | 10 | 12 | 8 | 1 | 5 | 3 | 0 | 1 | 3 | 1 | 2 | 2 | 2 | 0 | 1 |
Nucleolus | 11 | 3 | 6 | 11 | 17 | 14 | 33 | 76 | 27 | 35 | 25 | 39 | 6 | 1 | 5 | 11 | 10 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 2 | 4 | 5 | 25 | 12 | 10 | 5 | 12 | 3 | 14 | 2 | 3 | 1 | 2 | 0 | 1 | 3 | 1 | 8 | 3 | 6 | 3 |
SpindlePole | 2 | 1 | 2 | 2 | 3 | 7 | 12 | 8 | 7 | 12 | 3 | 10 | 0 | 2 | 1 | 3 | 7 | 2 | 1 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 58 | 39 | 21 | 20 | 22 | 25 | 39 | 41 | 34 | 29 | 20 | 32 | 56 | 93 | 94 | 25 | 27 | 31 | 9 | 19 | 5 | 6 | 8 | 4 |
Unique Cell Count | 479 | 479 | 587 | 374 | 635 | 781 | 985 | 1042 | 737 | 854 | 748 | 975 | 449 | 473 | 436 | 266 | 248 | 254 | 529 | 546 | 635 | 404 | 429 | 399 |
Labelled Cell Count | 788 | 770 | 851 | 516 | 827 | 978 | 1161 | 1250 | 887 | 1034 | 895 | 1201 | 664 | 669 | 585 | 335 | 324 | 344 | 529 | 546 | 635 | 404 | 429 | 399 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.5 | 15.5 | 11.1 | 10.5 | 9.1 | 9.0 | 8.3 | 8.8 | 9.3 | 8.7 | 8.8 | 8.8 | 14.7 | 13.8 | 13.0 | 15.2 | 14.8 | 15.1 | 15.6 | 14.4 | 14.3 |
Std Deviation (1e-4) | 2.6 | 3.3 | 1.9 | 1.9 | 1.7 | 1.7 | 1.8 | 1.9 | 2.5 | 1.8 | 1.8 | 1.7 | 2.6 | 2.5 | 2.5 | 3.5 | 3.3 | 3.6 | 2.7 | 2.8 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.29 | -0.3 | -0.42 | -0.34 | -0.26 | -0.36 | -0.34 | -0.33 | 0.41 | 0.31 | 0.23 | 0.46 | 0.42 | 0.45 | 0.49 | 0.38 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.3 | -1.1 | 1.6 | -1.0 | -0.2 | -0.5 | 0.2 | 0.5 | 1.1 | 3.7 | -0.2 | -0.6 | 6.4 | 5.9 | 8.3 |
Bud | -0.1 | -0.2 | 3.9 | 1.0 | 0.3 | 1.5 | 1.8 | 1.6 | 1.2 | -3.0 | -2.7 | -3.0 | -1.7 | -1.2 | -1.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.4 | 3.5 | 1.7 | 0.8 | 0.1 | 2.7 | 1.3 | 0 | 1.4 | 0.8 | 0.4 | 1.8 | 2.5 | 5.3 | 3.2 |
Endoplasmic Reticulum | 0 | 0.9 | 0.3 | 0.7 | -1.2 | 1.1 | 0.7 | 1.5 | 2.3 | 1.7 | 1.2 | 0 | 0 | 2.1 | 0 |
Endosome | 0.2 | -1.7 | -1.5 | -2.5 | -2.8 | -5.2 | -2.3 | -6.1 | -6.8 | -2.6 | -2.4 | -1.6 | 0.1 | -0.2 | 0.1 |
Golgi | -1.8 | -7.1 | -15.5 | -17.3 | -15.6 | -13.6 | -14.9 | -16.0 | -16.5 | 13.1 | 12.1 | 11.8 | 9.4 | 8.2 | 9.2 |
Mitochondria | -2.8 | 0 | 0.8 | 1.6 | 0.7 | 0.4 | 0.4 | 5.3 | 3.8 | -15.9 | -17.5 | -20.0 | -21.3 | -20.8 | -20.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.2 | 2.1 | 1.2 | 2.9 | 5.6 | 3.1 | 3.5 | 2.8 | 3.4 | 0.5 | 0 | 0 | 3.0 | 0 | 0 |
Peroxisomes | 0 | 0 | 3.8 | 1.8 | 0 | 0 | 2.0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1.8 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.3 | -0.1 | -0.4 | 0.4 | 0.4 | 0.9 | -0.2 | -0.9 | -0.3 | 5.4 | 8.4 | 9.0 | 3.5 | 4.1 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.6325 | 9.7841 | 9.0556 | 9.6097 | 9.1419 | 9.2449 | 12.1015 | 14.1081 | 13.2642 | 13.4941 | 12.8299 | 13.5291 | 9.2625 | 11.7092 | 10.3713 | 9.5734 | 10.699 | 10.1877 |
Actin | 0.0049 | 0.014 | 0.0023 | 0.0011 | 0.0005 | 0.0045 | 0.0049 | 0.0014 | 0.0016 | 0.0001 | 0.0006 | 0.0017 | 0.0093 | 0.0019 | 0.0034 | 0.0013 | 0.0004 | 0.0046 |
Bud | 0.0001 | 0.0024 | 0.0007 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Periphery | 0.0001 | 0.0006 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0002 | 0.0023 | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0039 | 0.0033 | 0.0021 | 0.0008 | 0.001 | 0.002 | 0.0008 | 0.001 | 0.001 | 0.0002 | 0.0005 | 0.0006 | 0.0011 | 0.0062 | 0.0008 | 0.0013 | 0.0003 | 0.0005 |
Cytoplasmic Foci | 0.0528 | 0.0593 | 0.0569 | 0.0377 | 0.0536 | 0.0372 | 0.0424 | 0.0454 | 0.0351 | 0.0166 | 0.0303 | 0.0274 | 0.0411 | 0.0349 | 0.0285 | 0.0204 | 0.028 | 0.0185 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0032 | 0.0005 | 0.0014 | 0.0003 | 0.0003 | 0.0044 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0012 | 0.0017 | 0.0013 | 0.0007 | 0.0013 | 0.0002 | 0.0009 |
Endosome | 0.1914 | 0.1676 | 0.1518 | 0.1685 | 0.1801 | 0.1122 | 0.103 | 0.084 | 0.0884 | 0.1447 | 0.0703 | 0.074 | 0.1609 | 0.141 | 0.1256 | 0.1966 | 0.1244 | 0.1109 |
Golgi | 0.6798 | 0.7243 | 0.7488 | 0.7607 | 0.7606 | 0.801 | 0.8203 | 0.8599 | 0.8445 | 0.7986 | 0.8897 | 0.8764 | 0.6958 | 0.7726 | 0.8017 | 0.7316 | 0.8288 | 0.8281 |
Lipid Particles | 0.01 | 0.0026 | 0.0041 | 0.0203 | 0.0012 | 0.0026 | 0.0031 | 0.0005 | 0.0009 | 0.0018 | 0.0008 | 0.0014 | 0.0242 | 0.0028 | 0.003 | 0.0055 | 0.0009 | 0.003 |
Mitochondria | 0.0415 | 0.0171 | 0.0215 | 0.0071 | 0.0008 | 0.028 | 0.0191 | 0.0063 | 0.0261 | 0.0299 | 0.0068 | 0.0152 | 0.0552 | 0.0336 | 0.033 | 0.0311 | 0.0149 | 0.0265 |
None | 0.0006 | 0.0004 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0018 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0003 |
Nucleolus | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0001 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0055 | 0.0012 | 0.0026 | 0.0004 | 0.0003 | 0.0038 | 0.0042 | 0.0005 | 0.0009 | 0.0003 | 0.0002 | 0.0009 | 0.0027 | 0.0005 | 0.0007 | 0.0004 | 0.0007 | 0.0005 |
Punctate Nuclear | 0.0007 | 0.0001 | 0.0008 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0018 | 0.0017 | 0.0021 | 0.0009 | 0.0005 | 0.0008 | 0.0004 | 0.0002 | 0.0003 | 0.0018 | 0.0001 | 0.0003 | 0.0015 | 0.0019 | 0.0006 | 0.0024 | 0.0002 | 0.0016 |
Vacuole Periphery | 0.0021 | 0.0018 | 0.0024 | 0.0019 | 0.0008 | 0.0016 | 0.0011 | 0.0004 | 0.0008 | 0.0055 | 0.0005 | 0.0007 | 0.0034 | 0.002 | 0.0014 | 0.0074 | 0.0011 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 75.9391 | 114.5047 | 67.367 | 81.8695 | 58.126 | 41.149 | 99.0295 | 89.5827 | 64.0967 | 87.6046 |
Translational Efficiency | 0.6868 | 0.57 | 0.8753 | 0.4877 | 0.7193 | 1.1331 | 0.7342 | 0.6712 | 0.7933 | 0.5372 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|