Standard name
Human Ortholog
Description Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.05 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.06 0.05 0 0.07 0 0 – 0 0.05 0 0.08 0.11 0.1 – 0.16 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0.66 0.71 0.48 0.13 0.12 0.06 0.11 0 – 0.08 0 0 0.73 0.81 0.8 – 0 0 0.85 0.72 0.85 0.63 0.62 0.62
Endosome 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.16 0.05 0.13 0.1 0.11 – 0.19 0.24 0.16 0 0 0 – 0.08 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 – 0.06 0.13 0.08 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0.17 0.09 0.22 0.21 0.35 0.28 0.38 0.24 – 0.38 0.25 0.35 0.16 0 0.05 – 0.25 0.19 0.08 0.12 0.08 0.25 0.22 0.15
Nucleolus 0 0 0 0 0 0 0 0.07 – 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.19 0.38 0.57 0.58 0.52 0.45 0.59 – 0.38 0.39 0.44 0.17 0.09 0.12 – 0.56 0.5 0 0.07 0 0.07 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 9 3 0 0 5 2 – 3 2 2 4 10 9 – 3 4 0 3 5 1 11 6
Bud 0 0 0 1 0 3 6 9 – 2 7 1 0 0 0 – 1 0 0 0 4 1 7 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 – 2 2 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 2 0 2 2 5 3 – 0 2 1 11 14 18 – 1 3 0 0 0 0 0 0
Cytoplasm 3 8 25 11 11 29 29 21 – 22 19 21 25 34 33 – 22 39 0 2 3 0 2 4
Endoplasmic Reticulum 63 72 189 29 48 26 72 19 – 44 7 21 217 244 271 – 3 6 100 101 442 154 405 314
Endosome 1 0 11 13 16 15 15 18 – 4 4 8 0 0 0 – 0 6 0 1 1 1 9 11
Golgi 5 0 3 1 4 0 2 0 – 0 0 0 0 4 0 – 2 10 2 1 2 0 6 2
Mitochondria 2 3 35 34 19 53 65 76 – 108 88 82 0 0 0 – 11 10 0 1 4 3 10 12
Nucleus 0 0 1 4 1 12 23 34 – 36 48 39 4 0 2 – 6 3 0 0 0 0 1 1
Nuclear Periphery 16 9 87 46 139 114 254 163 – 218 91 178 49 13 16 – 35 36 9 17 41 60 142 78
Nucleolus 4 1 0 4 6 10 12 51 – 26 9 12 0 0 0 – 1 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 3 1 2 – 0 0 1 0 1 0 – 1 1 0 0 0 0 0 0
Vac/Vac Membrane 23 19 150 125 230 210 298 403 – 216 143 225 51 28 42 – 78 92 2 9 11 17 50 66
Unique Cell Count 96 102 391 218 397 405 663 687 567 365 507 297 301 337 140 185 118 141 521 244 654 508
Labelled Cell Count 118 117 512 272 476 477 787 801 681 422 592 361 348 391 164 212 118 141 521 244 654 508


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 10.9 9.5 9.6 9.7 8.6 8.9 9.0 – 8.5 8.7 8.8 12.0 12.3 12.0 – 9.5 11.7 13.2 13.0 13.2
Std Deviation (1e-4) 2.4 2.1 2.1 2.4 2.2 1.9 1.9 2.0 – 1.8 2.0 2.0 2.0 2.3 2.1 – 2.7 2.9 2.4 2.8 2.8
Intensity Change (Log2) – – – 0.01 0.02 -0.15 -0.1 -0.09 – -0.16 -0.13 -0.12 0.34 0.37 0.33 – -0.01 0.3 0.47 0.45 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 -2.1 0 – 0 -2.0 0 -0.9 0.8 0.3 – 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 3.0 3.6 4.0 – 0 0
Cytoplasm -0.7 -2.4 0.4 -1.4 -2.6 – -1.8 -0.7 -1.5 1.0 2.3 1.7 – 3.3 5.2
Endoplasmic Reticulum -8.6 -11.1 -13.3 -13.6 -18.2 – -14.4 -14.6 -15.5 6.5 8.8 8.9 – -9.8 -10.7
Endosome 1.9 0.9 0.7 -0.6 -0.2 – -2.6 -1.7 -1.3 0 0 -3.1 – 0 0.3
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Mitochondria 2.5 -2.3 1.9 0.5 1.1 – 4.3 5.6 3.2 -5.3 -5.3 -5.6 – -0.4 -1.5
Nucleus 0 0 3.0 3.4 4.2 – 4.8 7.2 5.4 0 0 0 – 0 0
Nuclear Periphery -0.3 4.0 1.9 5.4 0.6 – 5.3 0.9 4.2 -1.9 -6.7 -6.8 – 0.7 -0.8
Nucleolus 0 0 0 0 5.5 – 4.3 0 3.1 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Vacuole 4.5 5.5 3.8 2.1 6.4 – -0.1 0.2 1.8 -6.1 -8.7 -7.9 – 3.6 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.6227 6.0979 6.2468 4.8562 5.476 5.7264 5.2869 6.5676 5.5684 5.7852 4.2707 4.9295 6.3065 6.2052 5.4099 4.6397 3.7748 5.314
Actin 0.0007 0.0007 0.0006 0.0037 0.0002 0.0002 0.001 0.0003 0.0031 0.0002 0.0058 0.0002 0.0007 0.001 0.0002 0.0004 0.0005 0.0002
Bud 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0 0 0 0 0 0
Bud Neck 0.0001 0.0004 0.0001 0.0024 0.0038 0.0003 0.0004 0 0.0003 0.0001 0.0004 0.0003 0.0001 0.0001 0.0001 0.0001 0.0006 0.0001
Bud Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0004 0 0.0004 0.0001 0.0003 0 0.0001 0 0 0 0.0001 0
Bud Site 0 0.0028 0.0002 0.003 0.0001 0 0.0006 0.0001 0.0058 0 0.0009 0 0 0.0002 0 0 0.0003 0
Cell Periphery 0.0001 0.0003 0.0001 0.0004 0 0 0.0002 0.0004 0.0006 0.0002 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0
Cytoplasm 0.0127 0.061 0.0178 0.032 0.0266 0.0094 0.0058 0.001 0.0077 0.0016 0.0346 0.0108 0.0127 0.0025 0.0035 0.0056 0.0281 0.0014
Cytoplasmic Foci 0.0008 0.0059 0.0019 0.0012 0.0356 0.0028 0.0024 0.008 0.0038 0.0001 0.0106 0.0003 0.0003 0.0033 0.0052 0.0006 0.0006 0.0001
Eisosomes 0.0001 0 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.3763 0.4509 0.5316 0.6618 0.4672 0.4438 0.5077 0.7326 0.6581 0.6816 0.5349 0.6054 0.5753 0.7705 0.7545 0.8541 0.7055 0.5562
Endosome 0.0096 0.0081 0.0058 0.0094 0.0431 0.0086 0.0231 0.0012 0.0145 0.005 0.0365 0.0048 0.0058 0.0048 0.0019 0.0087 0.0085 0.0017
Golgi 0.0003 0.0004 0.0003 0.0005 0.0031 0.0009 0.0014 0.0002 0.0106 0.0003 0.034 0.0001 0.0002 0.0018 0.0003 0.0006 0.0003 0.0001
Lipid Particles 0.0026 0.0021 0.0027 0.0014 0.0046 0.0061 0.0104 0.0014 0.0116 0.0006 0.0119 0.0007 0.0015 0.0313 0.0125 0.0019 0.0008 0.0014
Mitochondria 0.0005 0.0003 0.0004 0.0004 0.001 0.0014 0.001 0.0002 0.0087 0.0006 0.0076 0.0002 0.0005 0.0002 0.0002 0.0002 0.0002 0.0001
None 0.0222 0.0036 0.0114 0.0008 0.0011 0.0002 0.0009 0.0003 0.0018 0.0001 0.0126 0.0004 0.0015 0.0001 0.0001 0.0002 0.0004 0.0001
Nuclear Periphery 0.52 0.3816 0.3651 0.2579 0.3221 0.485 0.3885 0.1997 0.2342 0.2811 0.223 0.3587 0.3735 0.1644 0.2068 0.1171 0.2309 0.4237
Nucleolus 0.0003 0.0016 0.0006 0.0001 0.0003 0.0001 0.0026 0.0002 0.0007 0 0.0012 0.0001 0 0.0001 0 0 0.0001 0
Nucleus 0.0387 0.0584 0.0341 0.0134 0.0551 0.0252 0.0108 0.0034 0.0063 0.0042 0.0103 0.0083 0.0076 0.0026 0.003 0.0021 0.0099 0.0054
Peroxisomes 0.0002 0.0001 0.0001 0.0001 0.0043 0.0074 0.002 0.0001 0.0009 0 0.0017 0 0 0.0003 0 0 0 0
Punctate Nuclear 0.0017 0.0077 0.0112 0.0027 0.0073 0.0012 0.0158 0.0431 0.0092 0.0001 0.0639 0.0004 0.0004 0.0056 0.0009 0.0006 0.0007 0.0002
Vacuole 0.0045 0.0087 0.0061 0.003 0.0171 0.0022 0.0103 0.0028 0.0089 0.0056 0.0042 0.0035 0.0056 0.0052 0.0035 0.004 0.0071 0.002
Vacuole Periphery 0.0087 0.0052 0.0095 0.0054 0.0069 0.0051 0.0145 0.005 0.0124 0.0184 0.0051 0.0057 0.0139 0.0059 0.0069 0.0036 0.005 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 81.1972 65.9154 27.9497 40.5357 96.7399 50.0073 67.0437 50.8727 37.317 141.0793
Translational Efficiency 1.2877 1.2237 0.9942 0.7621 1.1906 1.821 1.1984 0.8028 1.1469 0.9877

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
Localization
Cell Percentages ER (68%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Lac1

Lac1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lac1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available