Standard name
Human Ortholog
Description Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0.07 0.15 0.26 0.14 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0.1 0.07 0.1 0 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.14 0 0 0.06 0.07 0.12 0.06 0.06 0.07 0.06 0.07 0.07 0.12 0 0 0 0 0 0.05
Nucleus 0.87 0.89 0.84 0.08 0.1 0.12 0.11 0.12 0.18 0.11 0.15 0.14 0.3 0.08 0.06 0.25 0.33 0.31 0.66 0.73 0.57 0.38 0.25 0.21
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.12 0.16 0.9 0.83 0.77 0.79 0.79 0.72 0.76 0.74 0.76 0.69 0.87 0.76 0.43 0.47 0.58 0.28 0.19 0.36 0.37 0.51 0.47
Peroxisomes 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.17 0.18 0.23 0.16 0.12 0.14 0.13 0.13 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0.05 0 0.21 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 2 0 0 2 0 1 0 0 0 0 0 1 1 1 3 4
Bud Neck 0 0 0 0 4 1 0 1 0 0 5 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 4 1 4 5 1 12 8 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 6 6 2 4 7 2 17 6 4 14 10 11 8 19 51 49 23 9 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 5 4 3 0 0 0 0 0 0
Endosome 0 0 0 6 23 5 32 22 25 11 38 48 0 3 6 1 0 0 0 0 1 0 1 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 2
Mitochondria 0 2 0 13 3 30 5 8 15 19 52 29 15 21 21 14 12 14 1 1 0 1 11 13
Nucleus 241 380 134 15 26 25 37 38 46 29 68 68 77 23 20 47 53 37 194 349 106 93 88 60
Nuclear Periphery 0 3 0 0 3 2 7 3 1 3 5 3 0 0 0 0 0 0 1 1 0 1 3 3
Nucleolus 44 51 25 162 214 160 256 248 184 194 331 362 179 252 262 82 76 69 83 91 67 89 181 136
Peroxisomes 0 0 0 1 11 7 19 10 5 7 16 13 2 1 6 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 11 44 38 74 50 30 35 57 60 2 8 17 1 2 0 0 1 0 1 6 5
Vac/Vac Membrane 7 6 3 0 6 4 6 4 5 3 8 9 1 0 5 10 13 4 10 23 7 52 57 50
Unique Cell Count 276 426 159 180 259 209 326 313 254 254 445 476 258 291 343 190 162 119 293 477 187 245 359 289
Labelled Cell Count 298 448 164 212 342 279 454 394 322 316 602 614 284 329 388 210 184 136 293 477 187 245 359 289


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.1 24.8 23.1 21.8 19.3 11.0 14.7 13.7 13.0 12.8 13.1 12.9 26.9 24.6 22.4 30.1 32.6 34.3 23.4 23.9 23.4
Std Deviation (1e-4) 7.4 7.5 5.3 6.7 8.2 4.0 4.4 4.2 3.6 3.7 4.6 4.1 9.4 10.2 12.9 20.8 18.9 17.0 7.9 9.0 7.0
Intensity Change (Log2) – – – -0.09 -0.27 -1.07 -0.65 -0.75 -0.83 -0.85 -0.83 -0.84 0.22 0.09 -0.04 0.38 0.49 0.57 0.02 0.04 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.1 0 0 2.2 0 0 0 2.5 4.6 6.5 4.3 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.9 0 4.1 3.4 4.1 0 3.8 4.2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.5 0 5.0 0 0 3.1 3.5 4.5 3.2 3.1 3.5 3.2 3.5 3.5 4.4
Nucleus -14.1 -15.2 -13.9 -15.8 -15.4 -13.2 -14.7 -15.8 -16.4 -10.8 -16.2 -17.7 -11.1 -9.4 -9.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 13.7 13.4 11.6 13.2 13.2 11.2 12.0 12.9 13.5 10.6 14.8 12.8 5.5 6.0 7.4
Peroxisomes 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.2 5.5 5.7 6.5 5.3 4.5 4.9 4.7 4.7 0 0 2.9 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.7 2.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.4188 26.8528 24.1648 20.5648 19.9499 23.4364 30.1265 34.8622 31.3838 28.313 29.9956 30.2205 30.1334 32.9984 29.6618 29.3721 24.7569 28.8015
Actin 0.0007 0.0001 0.0015 0.0002 0.0097 0.0003 0.0027 0 0 0.0092 0.0001 0.0031 0 0 0 0 0.0006 0
Bud 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0004 0.0006 0.0001 0.0001 0 0 0 0 0.0001 0
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0002
Bud Periphery 0.0004 0.0001 0.0001 0.0004 0.0004 0.0003 0.0003 0.0001 0.0002 0.0008 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0002
Bud Site 0.0014 0 0.0002 0.0004 0.0002 0.0001 0.0005 0.0001 0.0005 0.0013 0.0001 0.0001 0.0001 0 0 0 0.0005 0
Cell Periphery 0.0003 0.0002 0.0002 0.0005 0.0002 0.0008 0.0007 0.0008 0.0009 0.0005 0.0003 0.0009 0.0001 0.0004 0.0001 0.0001 0.0001 0.0004
Cytoplasm 0.0013 0 0.0004 0.0001 0.0219 0.0002 0.0021 0 0.0012 0.0164 0.0002 0.0001 0 0 0 0 0.0006 0
Cytoplasmic Foci 0.0084 0 0.0053 0.0003 0.0024 0.005 0.0073 0 0.0085 0.0018 0.0013 0.0024 0.0001 0 0 0.0002 0.0057 0
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0001 0 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0007 0 0.0002 0.0001 0.0006 0.0001 0.0004 0 0 0.002 0 0.0001 0 0 0 0 0.0001 0
Endosome 0.0099 0 0.0078 0.0003 0.0073 0.0026 0.0053 0 0.0011 0.0227 0 0.0004 0 0 0 0 0.0002 0
Golgi 0.001 0 0.0019 0 0.0133 0.0009 0.006 0 0.0004 0.0072 0 0.0004 0 0 0 0 0.0002 0
Lipid Particles 0.0061 0.0001 0.0071 0.0008 0.0107 0.0015 0.0058 0.0001 0.0013 0.0015 0.0008 0.0037 0.0006 0 0.0001 0.0001 0.0022 0.0004
Mitochondria 0.003 0.0006 0.0053 0.0042 0.0291 0.0104 0.0066 0.0007 0.0035 0.0018 0.005 0.0109 0.001 0.0004 0.0002 0.0007 0.0048 0.001
None 0.001 0 0.0022 0.0002 0.0266 0.0001 0.0012 0 0.0001 0.0002 0.0005 0.0001 0 0 0 0 0.0028 0
Nuclear Periphery 0.0071 0.0039 0.0036 0.0441 0.0634 0.0122 0.0076 0.0051 0.0028 0.0108 0.0081 0.0092 0.0003 0.0015 0.0014 0.0036 0.0016 0.0079
Nucleolus 0.716 0.6603 0.8025 0.7153 0.4405 0.756 0.632 0.6072 0.7075 0.5528 0.5166 0.6801 0.7622 0.7152 0.8425 0.566 0.6734 0.7332
Nucleus 0.2259 0.332 0.1514 0.2222 0.359 0.1913 0.3091 0.378 0.2654 0.3649 0.4598 0.2722 0.2349 0.2795 0.1547 0.4265 0.2943 0.2535
Peroxisomes 0.0004 0 0.001 0.0001 0.0023 0.0001 0.0013 0 0.0008 0.0002 0.0001 0.0035 0 0 0 0 0.0004 0
Punctate Nuclear 0.0104 0.0004 0.0047 0.0016 0.0045 0.0014 0.0023 0.0003 0.0004 0.0005 0.0007 0.0007 0.0003 0.0001 0.0004 0.0004 0.0106 0.0004
Vacuole 0.0025 0.0005 0.0009 0.0017 0.0011 0.0024 0.0028 0.0018 0.0028 0.0018 0.001 0.002 0.0002 0.0012 0.0001 0.0005 0.0003 0.0007
Vacuole Periphery 0.003 0.0015 0.0036 0.0073 0.0064 0.0141 0.0056 0.0056 0.0018 0.0022 0.0049 0.0094 0.0003 0.0014 0.0004 0.0014 0.0009 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 100.8738 91.5623 71.0315 92.5085 85.5148 72.8992 84.8637 55.8572 47.9361 94.718
Translational Efficiency 3.1464 3.3624 3.215 1.6574 2.6255 2.84 2.2467 2.4669 2.4111 2.0656

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus
Localization
Cell Percentages nucleus (88%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Mrt4

Mrt4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrt4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available