Standard name
Human Ortholog
Description Mitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0.06 0.05 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.78 0.9 0.9 0.9 0.89 0.74 0.63 0.65 0.51 0.57 0.47 0.49 0.99 0.97 0.97 0.59 0.59 0.51 0.41 0.48 0.36 0.53 0.48 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0.05 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0.12 0.06 0 0.05 0.05 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.05 0 0 0.05 0.22 0.4 0.32 0.55 0.45 0.65 0.58 0 0 0 0 0 0.08 0.27 0.31 0.34 0.27 0.25 0.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.07 0.06 0.08 0.08 0.1 0.09 0.07 0.07 0.09 0 0 0 0.24 0.27 0.17 0.05 0.05 0.07 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 1 0 0 0 1 0 0 0 0 0 0 0 2 1 6 4 7 7 6 0 7 6
Bud 5 5 1 3 2 8 13 19 14 15 14 12 0 2 1 1 7 6 2 1 4 1 3 7
Bud Neck 8 4 14 6 11 10 8 27 4 13 3 6 1 1 2 1 5 3 2 2 2 1 3 7
Bud Site 0 0 0 0 0 1 3 1 2 2 6 2 0 0 0 0 2 2 – – – – – –
Cell Periphery 0 2 0 0 0 2 2 2 1 1 0 1 0 1 6 6 7 4 3 1 3 0 5 2
Cytoplasm 161 194 162 193 273 264 267 229 123 175 144 135 91 127 188 79 73 74 104 123 74 73 126 152
Endoplasmic Reticulum 0 2 0 0 0 0 0 1 0 0 0 0 0 1 0 9 5 7 14 0 3 2 2 1
Endosome 0 2 2 0 4 2 2 1 0 0 0 2 0 0 1 15 6 18 14 10 9 6 13 10
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 1 0 0 0 3
Mitochondria 39 10 0 5 16 80 169 112 133 139 198 160 0 0 0 2 5 11 70 80 70 36 66 58
Nucleus 4 0 1 1 2 0 2 1 2 2 1 0 0 0 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 5 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 0 1 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 1 1
Peroxisomes 0 0 1 1 1 1 2 1 1 0 1 1 0 0 0 5 0 23 2 0 0 0 2 4
SpindlePole 4 3 11 14 17 30 35 34 22 23 22 26 1 1 2 32 33 24 12 13 13 5 14 18
Vac/Vac Membrane 1 3 1 0 3 4 2 3 2 1 4 5 0 0 0 5 2 4 8 7 2 1 7 8
Unique Cell Count 206 216 180 215 308 356 424 352 240 307 305 278 92 131 193 135 123 145 258 258 207 137 262 287
Labelled Cell Count 228 231 194 224 330 402 508 431 306 371 394 350 93 133 203 161 152 180 258 258 207 137 262 287


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.0 4.7 4.2 4.6 3.6 3.3 4.1 3.1 3.2 2.8 3.3 4.2 4.7 4.6 6.0 5.2 4.9 4.3 4.6 5.3
Std Deviation (1e-4) 0.5 0.8 1.7 1.6 2.6 1.6 1.5 1.7 1.2 1.0 0.7 0.7 0.7 1.0 0.9 1.8 1.0 0.6 1.4 1.3 2.0
Intensity Change (Log2) – – – -0.17 -0.01 -0.4 -0.52 -0.21 -0.58 -0.53 -0.73 -0.52 -0.17 0.0 -0.03 0.36 0.17 0.07 -0.13 -0.01 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5085 0.3526 0.471 0.4632 0.777 0.4877 -0.0118 0.5154 0.3095 0.2171 -0.4212 0.3692 13.9028 2.103 9.5278 -1.1171 -0.8999 -1.1149
Actin 0.0194 0.0036 0.0247 0.0412 0.0291 0.0105 0.0041 0.0026 0.0042 0.0337 0.0009 0.0059 0.029 0.0157 0.019 0.005 0.0016 0.0088
Bud 0.0021 0.0032 0.0023 0.0028 0.0012 0.0018 0.0011 0.0022 0.0021 0.0015 0.0004 0.0014 0.0045 0.0043 0.0009 0.0042 0.0026 0.0013
Bud Neck 0.001 0.002 0.0034 0.0038 0.0018 0.0038 0.0024 0.002 0.0015 0.012 0.0009 0.004 0.0017 0.0014 0.0026 0.0002 0.0009 0.0047
Bud Periphery 0.0091 0.0119 0.0063 0.0097 0.0018 0.0043 0.0029 0.0053 0.0047 0.0042 0.0007 0.0033 0.0058 0.0052 0.0018 0.0084 0.0039 0.001
Bud Site 0.0041 0.0208 0.0175 0.0097 0.0025 0.0018 0.0095 0.0458 0.0089 0.0041 0.001 0.0017 0.0115 0.0261 0.0061 0.0007 0.0205 0.0006
Cell Periphery 0.001 0.0023 0.0023 0.0011 0.0004 0.0017 0.0011 0.001 0.0007 0.0008 0.0003 0.0006 0.0014 0.0016 0.0011 0.0015 0.0005 0.0007
Cytoplasm 0.078 0.1305 0.1098 0.0891 0.0949 0.1053 0.0823 0.0765 0.0804 0.1347 0.0857 0.0958 0.0685 0.1154 0.1109 0.102 0.0684 0.1572
Cytoplasmic Foci 0.0976 0.1272 0.1217 0.0767 0.1648 0.1538 0.1155 0.1111 0.1099 0.1531 0.1392 0.139 0.1427 0.1552 0.1077 0.027 0.0558 0.1618
Eisosomes 0.001 0.0012 0.001 0.0012 0.0005 0.0005 0.0007 0.0005 0.0005 0.0012 0.0002 0.0003 0.0009 0.0006 0.0017 0.0003 0.0001 0.0003
Endoplasmic Reticulum 0.0059 0.0034 0.0038 0.0031 0.003 0.0044 0.0022 0.0027 0.0027 0.0029 0.0035 0.0015 0.0066 0.0033 0.0027 0.0013 0.0009 0.0029
Endosome 0.0859 0.0924 0.0562 0.058 0.0771 0.0988 0.0417 0.048 0.0477 0.0555 0.0975 0.0378 0.1109 0.087 0.0558 0.01 0.1231 0.0722
Golgi 0.027 0.0248 0.0235 0.0333 0.0527 0.0383 0.0068 0.0119 0.0098 0.0114 0.0236 0.009 0.0244 0.0132 0.0199 0.0021 0.0384 0.0159
Lipid Particles 0.0371 0.0107 0.0193 0.0292 0.0542 0.0185 0.0198 0.0165 0.0144 0.0063 0.0268 0.0144 0.0487 0.0148 0.0109 0.0015 0.0079 0.0168
Mitochondria 0.0729 0.0784 0.0923 0.092 0.0676 0.1639 0.0404 0.0901 0.066 0.0429 0.0464 0.063 0.0547 0.026 0.048 0.0418 0.0535 0.0473
None 0.502 0.4343 0.4507 0.4658 0.3629 0.3193 0.6214 0.5418 0.5884 0.4596 0.5266 0.5334 0.3401 0.4551 0.5341 0.6751 0.5757 0.4351
Nuclear Periphery 0.0024 0.0018 0.0014 0.0015 0.0016 0.0014 0.0023 0.0012 0.0017 0.0009 0.0013 0.0015 0.0089 0.0033 0.0008 0.0012 0.0008 0.0007
Nucleolus 0.0009 0.0009 0.0013 0.001 0.0004 0.0007 0.002 0.0022 0.0025 0.0012 0.0012 0.0014 0.0173 0.0067 0.0111 0.0141 0.0075 0.0053
Nucleus 0.0014 0.0015 0.0014 0.0016 0.0008 0.0011 0.0032 0.0027 0.0034 0.004 0.0026 0.0034 0.0681 0.0217 0.038 0.0111 0.0022 0.0022
Peroxisomes 0.0393 0.0408 0.0512 0.0708 0.0736 0.0603 0.0249 0.0259 0.0365 0.058 0.0306 0.0664 0.0265 0.0262 0.0202 0.0829 0.0242 0.0573
Punctate Nuclear 0.0028 0.0012 0.0035 0.0017 0.0043 0.0021 0.0069 0.0037 0.0082 0.0057 0.0032 0.0119 0.0161 0.0046 0.0021 0.0009 0.0019 0.0024
Vacuole 0.0067 0.0055 0.0049 0.0047 0.0032 0.0055 0.0078 0.0051 0.0042 0.0045 0.0056 0.0033 0.009 0.0107 0.0034 0.0066 0.0072 0.0039
Vacuole Periphery 0.0024 0.0015 0.0015 0.0019 0.0017 0.0022 0.0011 0.0012 0.0016 0.0017 0.0017 0.0011 0.0025 0.0019 0.0011 0.0022 0.0026 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3119 10.2167 2.0676 7.0941 8.7561 10.3841 10.7996 9.5074 9.4293 9.6666
Translational Efficiency 0.6286 0.7026 2.065 0.8127 0.6776 0.4739 0.7012 0.5283 0.629 0.7612

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (11%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cce1

Cce1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cce1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available