Standard name | |
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Human Ortholog | |
Description | Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 2 | 1 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 6 | – | – | 0 | 4 | 8 | 1 | 4 | 13 | 2 | 0 | 4 | 3 |
Bud | 5 | 4 | 8 | 14 | 13 | 10 | 21 | 21 | 21 | 15 | 18 | 28 | – | – | 0 | 4 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 4 | 0 | 0 | 2 | 1 | 5 | 7 | 7 | 6 | 7 | 4 | 9 | – | – | 0 | 1 | 4 | 2 | – | – | – | – | – | – |
Cell Periphery | 5 | 9 | 0 | 2 | 0 | 0 | 2 | 0 | 2 | 1 | 5 | 0 | – | – | 0 | 3 | 9 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 2 | 7 | 9 | 8 | 8 | 4 | 5 | 5 | 3 | 5 | – | – | 0 | 10 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 2 | 2 | 0 | 1 | 3 | 0 | 2 | 1 | 1 | 5 | 0 | – | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 9 | 2 | 1 | 3 | 9 | 1 | 3 | 3 | 1 | 4 | 0 | 1 | – | – | 0 | 4 | 6 | 10 | 20 | 15 | 8 | 0 | 2 | 4 |
Golgi | 0 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | – | – | 0 | 10 | 2 | 8 | 13 | 39 | 9 | 2 | 7 | 5 |
Mitochondria | 239 | 301 | 173 | 275 | 399 | 411 | 687 | 506 | 513 | 688 | 598 | 883 | – | – | 12 | 6 | 7 | 1 | 173 | 216 | 123 | 11 | 16 | 20 |
Nucleus | 4 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 1 | 0 | 2 | 0 | – | – | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 20 | 23 | 14 | 17 | 34 | 53 | 82 | 39 | 46 | 69 | 37 | 58 | – | – | 1 | 10 | 6 | 8 | 2 | 3 | 1 | 0 | 0 | 0 |
Nucleolus | 25 | 5 | 5 | 8 | 30 | 40 | 102 | 77 | 69 | 72 | 67 | 88 | – | – | 3 | 12 | 8 | 11 | 1 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 23 | 21 | 15 | 41 | 16 | 19 | 23 | 26 | 19 | 38 | 12 | 40 | – | – | 1 | 63 | 44 | 30 | 51 | 37 | 30 | 3 | 7 | 5 |
SpindlePole | 5 | 0 | 3 | 7 | 1 | 1 | 6 | 12 | 9 | 10 | 7 | 7 | – | – | 0 | 15 | 3 | 4 | 5 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 26 | 5 | 5 | 8 | 7 | 8 | 15 | 6 | 5 | 3 | 8 | – | – | 1 | 4 | 5 | 5 | 1 | 3 | 0 | 0 | 0 | 0 |
Unique Cell Count | 266 | 324 | 180 | 291 | 434 | 442 | 751 | 579 | 563 | 754 | 635 | 945 | 17 | 124 | 94 | 85 | 281 | 334 | 179 | 21 | 42 | 45 | ||
Labelled Cell Count | 346 | 405 | 231 | 383 | 523 | 564 | 954 | 718 | 700 | 916 | 763 | 1135 | 18 | 150 | 119 | 94 | 281 | 334 | 179 | 21 | 42 | 45 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 9.0 | 6.9 | 7.7 | 8.0 | 8.6 | 8.9 | 9.7 | 11.7 | 13.2 | 14.8 | 15.1 | – | – | 8.5 | 11.0 | 11.2 | 11.9 | – | 9.5 | – |
Std Deviation (1e-4) | 1.7 | 2.0 | 1.5 | 1.8 | 2.0 | 2.3 | 2.5 | 3.3 | 4.2 | 5.0 | 6.4 | 6.8 | – | – | 1.2 | 3.5 | 4.1 | 3.3 | – | 1.8 | – |
Intensity Change (Log2) | – | – | – | 0.15 | 0.21 | 0.31 | 0.37 | 0.49 | 0.76 | 0.93 | 1.1 | 1.13 | – | – | 0.29 | 0.67 | 0.7 | 0.78 | – | 0.46 | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Bud | 0.2 | -0.9 | -1.5 | -1.1 | -0.5 | -0.4 | 0 | -1.1 | -1.0 | – | – | 0 | 0 | 1.7 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Mitochondria | -0.8 | -1.9 | -1.5 | -2.1 | -3.3 | -2.2 | -2.2 | -1.0 | -1.4 | – | – | -4.2 | -15.9 | -14.7 | -15.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.8 | 0 | 1.5 | 1.2 | -0.5 | 0.2 | 0.6 | -1.0 | -0.8 | – | – | 0 | 0.1 | -0.4 | 0.4 |
Nucleolus | 0 | 2.0 | 2.7 | 4.1 | 4.0 | 3.7 | 3.0 | 3.2 | 2.9 | – | – | 0 | 2.6 | 0 | 3.2 |
Peroxisomes | 1.9 | -2.4 | -2.0 | -3.2 | -2.0 | -2.8 | -1.7 | -4.3 | -2.3 | – | – | 0 | 8.3 | 7.4 | 5.5 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 3.8 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.0456 | 30.8067 | 28.8496 | 24.8335 | 26.3056 | 27.2723 | 20.0653 | 25.6489 | 23.8418 | 22.8825 | 21.0388 | 24.4355 | 23.9834 | 27.7783 | 26.5555 | 21.6162 | 23.9548 | 24.732 |
Actin | 0.0092 | 0.0154 | 0.0107 | 0.0063 | 0.0019 | 0.0036 | 0.028 | 0.0038 | 0.0199 | 0.0041 | 0.0074 | 0.0015 | 0.0068 | 0.0075 | 0.0059 | 0.001 | 0.0013 | 0.0006 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0022 | 0.0001 | 0.0046 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Periphery | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0032 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0014 | 0.0011 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.017 | 0.0016 | 0.0027 | 0.0001 | 0.0011 | 0.001 | 0.0023 | 0.0011 | 0.0008 | 0.0022 | 0.003 | 0.0003 |
Eisosomes | 0.0007 | 0.0015 | 0.0033 | 0.0004 | 0.0003 | 0.0008 | 0.0007 | 0.0004 | 0.0009 | 0.0044 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0012 | 0.0002 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0025 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0009 | 0.0016 | 0.0011 | 0.001 | 0.0004 | 0.0018 | 0.0153 | 0.0005 | 0.0036 | 0.0006 | 0.0047 | 0.0002 | 0.002 | 0.0018 | 0.0008 | 0.0009 | 0.0012 | 0.0008 |
Golgi | 0.008 | 0.0203 | 0.0138 | 0.0196 | 0.0034 | 0.0205 | 0.0162 | 0.0103 | 0.0093 | 0.0032 | 0.0056 | 0.0023 | 0.0062 | 0.0208 | 0.0104 | 0.0103 | 0.0044 | 0.0098 |
Lipid Particles | 0.023 | 0.0296 | 0.017 | 0.007 | 0.0396 | 0.0202 | 0.0526 | 0.0218 | 0.0146 | 0.007 | 0.0361 | 0.0179 | 0.0398 | 0.0319 | 0.0399 | 0.0303 | 0.0542 | 0.022 |
Mitochondria | 0.9344 | 0.9026 | 0.9354 | 0.9607 | 0.9323 | 0.9317 | 0.8013 | 0.9322 | 0.9117 | 0.9697 | 0.872 | 0.9537 | 0.9137 | 0.9047 | 0.922 | 0.9265 | 0.9071 | 0.9489 |
None | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0037 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0036 | 0.0054 | 0 | 0.0004 | 0 | 0.0023 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0034 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.005 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0001 |
Nucleus | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0.001 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0105 | 0.0098 | 0.011 | 0.0024 | 0.0123 | 0.0025 | 0.0366 | 0.0261 | 0.0134 | 0.0077 | 0.0204 | 0.0223 | 0.0129 | 0.0112 | 0.0076 | 0.0271 | 0.0251 | 0.0039 |
Punctate Nuclear | 0.0002 | 0 | 0 | 0 | 0 | 0.0007 | 0.0025 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0026 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0005 | 0.0011 | 0.0003 | 0.0002 | 0.0003 | 0.0013 | 0.0014 | 0.0003 | 0.0012 | 0.0002 | 0.0016 | 0.0001 | 0.0004 | 0.0015 | 0.0006 | 0.0001 | 0.0004 | 0.0005 |
Vacuole Periphery | 0.0067 | 0.0157 | 0.0063 | 0.002 | 0.0086 | 0.0113 | 0.0072 | 0.0022 | 0.0096 | 0.0024 | 0.0458 | 0.0007 | 0.0098 | 0.0181 | 0.0102 | 0.0013 | 0.0023 | 0.0127 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 102.9844 | 69.516 | 73.8904 | 121.8841 | 83.1118 | 75.6201 | 89.7592 | 87.827 | 86.3358 | 93.1895 |
Translational Efficiency | 2.0715 | 2.5233 | 2.6405 | 1.4559 | 2.5496 | 2.8795 | 1.8298 | 1.9139 | 2.1396 | 2.1825 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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