Standard name | |
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Human Ortholog | |
Description | ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | – | – | 4 | 11 | 5 | 29 | 13 | 9 | 9 |
Bud | 2 | 7 | 3 | 8 | 2 | 8 | 10 | 18 | – | 21 | 8 | 20 | 1 | 0 | 0 | – | – | 2 | 0 | 1 | 2 | 1 | 3 | 3 |
Bud Neck | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | – | – | 2 | 9 | 9 | 8 | 8 | 6 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 2 | 2 | 0 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 6 | 11 | 10 | 5 | 4 | 6 | 2 | – | 0 | 3 | 7 | 11 | 11 | 10 | – | – | 5 | 5 | 8 | 18 | 22 | 19 | 21 |
Cytoplasm | 276 | 176 | 234 | 194 | 243 | 236 | 262 | 256 | – | 222 | 136 | 164 | 161 | 225 | 270 | – | – | 251 | 193 | 94 | 67 | 115 | 135 | 231 |
Endoplasmic Reticulum | 12 | 2 | 9 | 3 | 2 | 3 | 3 | 3 | – | 2 | 6 | 3 | 8 | 1 | 0 | – | – | 16 | 72 | 45 | 116 | 58 | 36 | 38 |
Endosome | 1 | 1 | 6 | 1 | 5 | 4 | 6 | 7 | – | 1 | 2 | 3 | 0 | 0 | 0 | – | – | 1 | 1 | 6 | 4 | 1 | 3 | 3 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 2 | 0 | 0 | 1 |
Mitochondria | 4 | 8 | 16 | 50 | 25 | 127 | 195 | 260 | – | 301 | 209 | 242 | 0 | 0 | 0 | – | – | 8 | 26 | 23 | 50 | 63 | 36 | 51 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | – | 2 | 2 | 0 | 0 | 0 | 1 | – | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | – | 2 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 3 | – | 4 | 7 | 4 | 1 | 0 | 1 | – | – | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 1 | 1 | 2 | 5 | 7 | 8 | 19 | 4 | – | 8 | 8 | 7 | 0 | 0 | 0 | – | – | 1 | 6 | 10 | 8 | 12 | 8 | 16 |
Unique Cell Count | 294 | 189 | 269 | 232 | 272 | 314 | 421 | 461 | 435 | 308 | 354 | 172 | 226 | 272 | 260 | 333 | 212 | 318 | 303 | 266 | 396 | |||
Labelled Cell Count | 309 | 206 | 283 | 273 | 292 | 394 | 510 | 560 | 565 | 385 | 452 | 184 | 238 | 282 | 291 | 333 | 212 | 318 | 303 | 266 | 396 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.0 | 4.5 | 4.6 | 4.7 | 3.6 | 3.4 | 3.4 | – | 2.8 | 3.0 | 3.1 | 5.5 | 5.5 | 5.5 | 4.0 | 4.3 | 5.3 | 5.9 | 6.1 | 6.2 |
Std Deviation (1e-4) | 0.5 | 1.2 | 1.7 | 2.1 | 2.4 | 1.7 | 1.3 | 1.0 | – | 0.8 | 0.7 | 0.9 | 0.6 | 0.6 | 0.7 | 1.0 | 0.9 | 1.4 | 1.3 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.06 | -0.31 | -0.4 | -0.4 | – | -0.67 | -0.6 | -0.54 | 0.29 | 0.3 | 0.28 | -0.16 | -0.05 | 0.23 | 0.4 | 0.45 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.1 | 0.4 | -0.2 | – | – | -1.5 |
Cytoplasm | -1.1 | 0.8 | -3.6 | -7.1 | -8.7 | – | -9.7 | -10.7 | -10.5 | 2.2 | 5.4 | 5.7 | – | – | 4.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.7 | 0 | 0 | – | – | 1.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.3 | -3.7 | -4.1 | – | – | -1.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | 0 | 0 | 0 | 2.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.4817 | 0.0044 | 0.0061 | 0.0006 | 0.0129 | 0.0135 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0.0035 | 0.0005 | 0.0007 | 0.0009 | 0.0001 | 0.0021 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | 0.0116 | 0.0002 | 0.0018 | 0.0023 | 0.0006 | 0.011 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | 0.0076 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0028 | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | 0.0028 | 0.0078 | 0.0202 | 0.0087 | 0.0002 | 0.0102 | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0.0012 | 0.001 | 0.0004 | 0.0003 | 0.0004 | 0.0026 | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | 0.0085 | 0.5898 | 0.322 | 0.3315 | 0.5634 | 0.5496 | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0039 | 0.0133 | 0.0663 | 0.1401 | 0.0117 | 0.0236 | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | 0.0036 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0005 | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0556 | 0.0228 | 0.0127 | 0.019 | 0.0337 | 0.0194 | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | 0.0896 | 0.004 | 0.2306 | 0.2314 | 0.0395 | 0.0261 | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | 0.0249 | 0.0008 | 0.0233 | 0.0324 | 0.0057 | 0.0035 | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | 0.0052 | 0.0015 | 0.0663 | 0.0031 | 0.0025 | 0.0169 | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | 0.0176 | 0.0004 | 0.0033 | 0.003 | 0.0053 | 0.0167 | – | – | – | – | – | – | – | – | – | – | – | – |
None | 0.0191 | 0.3438 | 0.1434 | 0.0305 | 0.1278 | 0.1656 | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | 0.2122 | 0.0028 | 0.005 | 0.003 | 0.1739 | 0.0023 | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0.0012 | 0.0002 | 0.0057 | 0.0012 | 0.0005 | 0.0723 | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | 0.0216 | 0.0018 | 0.0193 | 0.0024 | 0.0078 | 0.0233 | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | 0.0012 | 0.0002 | 0.0106 | 0.0054 | 0.0001 | 0.0055 | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | 0.0049 | 0.0015 | 0.0203 | 0.001 | 0.0034 | 0.0114 | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | 0.0067 | 0.0023 | 0.0347 | 0.1618 | 0.0032 | 0.0179 | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | 0.0157 | 0.0003 | 0.0068 | 0.0209 | 0.0069 | 0.0032 | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9618 | 17.0013 | 12.6157 | 19.2374 | 16.9525 | 15.953 | 20.2596 | 17.7139 | 19.6285 | 22.6236 |
Translational Efficiency | 0.7215 | 0.7508 | 0.9605 | 0.6135 | 0.6373 | 0.7876 | 0.583 | 0.7786 | 0.6577 | 0.6146 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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