Standard name
Human Ortholog
Description Protein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.11 0.14 0 0.12 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.1 0 0.15 0.19 0.17 0.11 0.11 0.08 0.13 0.14 0 0 0 0.12 0 0.05 0 0 0 0 0.08 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0 0 0 0.1 0.08 0.12 0.14 0.19 0.19 0.15 0 0 0 0.12 0.25 0 0 0 0 0 0 0
Nucleus 0.44 0.41 0.21 0 0.11 0.06 0.07 0.07 0.08 0.07 0.06 0.08 0.06 0.05 0.05 0.19 0 0.05 0.24 0.27 0.09 0.09 0 0
Nuclear Periphery 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleolus 0.54 0.58 0.72 0.9 0.78 0.77 0.75 0.7 0.75 0.73 0.77 0.73 0.9 0.84 0.78 0.69 0.75 0.86 0.64 0.61 0.58 0.62 0.51 0.46
Peroxisomes 0 0 0 0.07 0.05 0.09 0.1 0.07 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.11 0.48 0.28 0.39 0.47 0.39 0.33 0.37 0.33 0.38 0.46 0.44 0.39 0 0 0.05 0 0 0.13 0.15 0.21 0.19
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0.05 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 1 0 1 1 7 4 5 6 2 0 0 0 0 0 0 3 0 4 1 5 4
Bud Neck 0 0 1 13 5 3 3 2 1 0 2 0 2 11 15 0 0 0 0 0 2 0 3 4
Bud Site 0 0 0 2 0 5 5 8 13 9 14 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 0 11 7 11 16 7 22 14 5 11 10 4 29 50 0 3 1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Endosome 3 0 33 9 39 81 81 59 32 25 56 57 1 5 10 2 1 1 5 4 13 5 27 43
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4 0 0 1
Mitochondria 4 16 10 5 7 41 39 68 42 62 82 64 1 0 1 2 6 0 1 1 7 4 9 10
Nucleus 105 48 72 11 28 24 33 41 25 23 28 34 6 14 17 3 1 1 68 34 35 16 15 9
Nuclear Periphery 8 2 22 0 3 3 4 3 3 6 2 6 0 1 0 0 1 0 6 1 22 3 5 7
Nucleolus 131 68 244 232 202 330 345 391 227 239 342 306 87 233 274 11 18 18 179 76 230 114 183 144
Peroxisomes 0 0 1 17 13 39 46 40 17 16 14 26 2 3 2 0 0 0 0 0 0 0 3 4
SpindlePole 3 0 37 124 72 169 218 221 101 119 146 160 45 121 136 0 1 1 4 2 51 27 75 59
Vac/Vac Membrane 9 2 10 0 8 5 10 5 8 5 5 7 0 1 3 2 2 1 8 2 13 6 16 17
Unique Cell Count 241 118 337 259 259 430 463 561 304 326 443 417 97 276 351 16 24 21 283 126 395 183 358 317
Labelled Cell Count 268 136 441 421 388 718 792 867 487 514 708 676 148 418 508 20 33 23 283 126 395 183 358 317


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 10.9 11.2 10.8 9.8 7.2 7.0 7.1 6.8 6.8 6.7 6.9 10.8 11.1 10.6 15.0 16.0 17.7 12.8 12.8 12.8
Std Deviation (1e-4) 3.1 2.4 3.5 3.3 4.2 2.6 2.6 2.8 2.2 2.5 2.1 2.5 2.5 4.3 3.6 6.1 7.4 4.2 4.6 3.6 4.0
Intensity Change (Log2) – – – -0.06 -0.19 -0.63 -0.67 -0.65 -0.72 -0.73 -0.75 -0.71 -0.05 -0.02 -0.09 0.42 0.51 0.66 0.19 0.2 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 3.8 0 0 0 0 0 0 0 0 0 3.3 3.5 0 0 0
Bud Site 0 0 0 0 0 3.8 0 3.3 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 0.6 0.3 -1.7 0.5 0.9 -1.5 -0.7 -0.7 0 3.6 5.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.0 2.0 3.5 3.1 0.3 0.3 -1.0 1.2 1.6 -2.8 -4.1 -3.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.8 -0.2 3.6 3.2 4.7 5.0 6.6 6.7 5.7 0 0 -2.8 0 0 0
Nucleus -6.0 -3.4 -6.6 -5.9 -6.1 -4.6 -5.3 -6.2 -5.2 -3.4 -5.8 -6.5 0 0 0
Nuclear Periphery -4.2 -3.2 -4.5 -4.5 -5.3 -3.6 -3.0 -4.9 -3.7 0 -4.0 -4.9 0 0 0
Nucleolus 5.2 1.6 1.4 0.7 -0.9 0.6 0.3 1.5 0.3 3.5 3.6 1.7 -0.3 0.3 1.3
Peroxisomes 4.4 3.8 5.4 5.7 4.7 4.1 3.8 2.9 4.4 0 0 0 0 0 0
SpindlePole 10.1 5.3 8.8 10.8 9.1 6.8 7.7 7.2 8.5 7.9 9.3 8.4 0 0 0
Vacuole 0 0.1 -1.8 -0.7 -2.4 -0.3 -1.2 -1.9 -1.2 0 0 -2.0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0101 0 0.0021 0.0193 0 0.0272 0.0228 0 0.0021 0 0.001 0 – – – – – –
Bud 0.0002 0.0001 0.0003 0.0007 0 0.0002 0.0002 0 0.0001 0 0.0002 0 – – – – – –
Bud Neck 0.0006 0.0005 0.0003 0.0012 0.0002 0.0008 0.0039 0.0003 0.0029 0.0008 0.0006 0.0004 – – – – – –
Bud Periphery 0.0003 0.0002 0.0003 0.0017 0.0001 0.0006 0.0004 0.0001 0.0002 0.0002 0.0004 0.0002 – – – – – –
Bud Site 0.0011 0.0004 0.0015 0.0009 0.0001 0.0003 0.0047 0.0002 0.0025 0.0003 0.0009 0.0001 – – – – – –
Cell Periphery 0.0002 0.0004 0.0001 0.0003 0 0.0005 0.0007 0.0001 0.0003 0.0002 0.0005 0.0002 – – – – – –
Cytoplasm 0.0066 0.0001 0.0031 0.0007 0.0001 0.0001 0.0005 0 0.001 0 0.0009 0 – – – – – –
Cytoplasmic Foci 0.0301 0.002 0.0017 0.0015 0.0128 0.0023 0.0202 0.0018 0.0159 0.0085 0.015 0.0005 – – – – – –
Eisosomes 0.0001 0 0 0.0003 0 0.0002 0.0003 0 0.0001 0 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0074 0 0.0007 0.0094 0 0.0004 0.0006 0 0.0002 0 0.0006 0 – – – – – –
Endosome 0.0189 0.0004 0.0038 0.0069 0.0004 0.0057 0.0051 0.0001 0.0055 0.0004 0.0057 0.0001 – – – – – –
Golgi 0.0065 0.0001 0.0019 0.0015 0.0003 0.0109 0.0038 0.0002 0.0044 0.0004 0.003 0.0002 – – – – – –
Lipid Particles 0.0215 0.0022 0.0011 0.002 0.0338 0.0068 0.028 0.006 0.0288 0.0221 0.1119 0.0066 – – – – – –
Mitochondria 0.0114 0.0004 0.001 0.006 0.001 0.0272 0.0034 0.002 0.0187 0.0022 0.0044 0.0003 – – – – – –
None 0.0022 0.0001 0.0005 0.0009 0.0002 0.0001 0.0008 0 0.0075 0.0001 0.0013 0 – – – – – –
Nuclear Periphery 0.0083 0.0014 0.0054 0.0094 0.0792 0.0233 0.0083 0.0206 0.0017 0.0065 0.0672 0.0119 – – – – – –
Nucleolus 0.6731 0.768 0.8524 0.7823 0.5858 0.7279 0.7727 0.8705 0.8219 0.881 0.541 0.8782 – – – – – –
Nucleus 0.1277 0.1982 0.0968 0.131 0.2553 0.1534 0.0814 0.0759 0.0584 0.0614 0.1456 0.0808 – – – – – –
Peroxisomes 0.0122 0.0001 0.0002 0.0002 0.0005 0.0016 0.0047 0.0001 0.002 0.001 0.0024 0.0001 – – – – – –
Punctate Nuclear 0.0594 0.0175 0.0259 0.0212 0.0282 0.0064 0.0312 0.0185 0.0231 0.0128 0.0854 0.0175 – – – – – –
Vacuole 0.0013 0.0068 0.0006 0.0013 0.0004 0.0014 0.0022 0.0003 0.0014 0.0008 0.0035 0.0005 – – – – – –
Vacuole Periphery 0.0008 0.0013 0.0002 0.0012 0.0015 0.0025 0.004 0.0033 0.0012 0.001 0.0086 0.0026 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.2715 28.743 27.0509 27.5834 29.9951 24.2975 25.3407 21.0502 20.4777 26.3502
Translational Efficiency 2.7452 2.1372 1.9547 1.8752 1.5225 1.8554 1.7744 1.5866 1.3349 1.5411

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
Localization
Cell Percentages nucleolus (28%), nucleus (13%), mixed (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mak11

Mak11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mak11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available