Standard name | |
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Human Ortholog | |
Description | Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Bud | 0 | 2 | 0 | 0 | 0 | 2 | 0 | – | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 0 | 0 | 3 | 0 | 6 | – | 5 | 6 | 7 | 4 | 1 | 5 | 3 | 11 | 12 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 357 | 502 | 91 | 135 | 144 | 92 | 222 | – | 194 | 163 | 155 | 133 | 312 | 368 | 486 | 346 | 433 | 443 | 362 | 482 | 101 | 143 | 300 | 328 |
Endoplasmic Reticulum | 0 | 6 | 0 | 0 | 0 | 1 | 4 | – | 1 | 5 | 6 | 4 | 3 | 0 | 1 | 22 | 15 | 21 | 0 | 4 | 0 | 2 | 3 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 2 | 0 | 0 | 0 | 10 | 2 | – | 29 | 8 | 20 | 16 | 0 | 1 | 1 | 4 | 4 | 2 | 1 | 0 | 0 | 1 | 0 | 0 |
Nucleus | 0 | 21 | 3 | 2 | 0 | 7 | 9 | – | 9 | 10 | 12 | 10 | 0 | 1 | 3 | 8 | 8 | 15 | 2 | 7 | 2 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 0 | 2 | 10 | 31 | 17 | 52 | – | 44 | 31 | 41 | 31 | 2 | 1 | 8 | 10 | 20 | 8 | 2 | 19 | 4 | 0 | 1 | 2 |
Unique Cell Count | 361 | 516 | 94 | 141 | 171 | 117 | 281 | 260 | 212 | 224 | 182 | 316 | 374 | 495 | 362 | 452 | 461 | 378 | 527 | 109 | 151 | 314 | 343 | |
Labelled Cell Count | 362 | 536 | 96 | 147 | 178 | 129 | 296 | 289 | 227 | 243 | 198 | 318 | 380 | 502 | 401 | 492 | 497 | 378 | 527 | 109 | 151 | 314 | 343 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.1 | 15.2 | 14.2 | 13.5 | 13.9 | 7.9 | 11.9 | – | 10.5 | 10.4 | 10.4 | 10.1 | 18.3 | 18.4 | 18.3 | 24.4 | 25.9 | 23.0 | 15.5 | 16.5 | 17.0 |
Std Deviation (1e-4) | 3.1 | 3.1 | 3.5 | 2.9 | 2.2 | 2.2 | 2.2 | – | 2.2 | 2.5 | 2.1 | 2.1 | 3.4 | 3.4 | 3.2 | 6.9 | 6.6 | 6.9 | 2.9 | 3.4 | 3.3 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.04 | -0.84 | -0.26 | – | -0.44 | -0.46 | -0.45 | -0.5 | 0.36 | 0.37 | 0.36 | 0.77 | 0.86 | 0.69 | 0.12 | 0.21 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -3.1 | -3.9 | -4.0 | – | -4.7 | -4.3 | -5.4 | -4.8 | 1.3 | 1.0 | 0.9 | -0.5 | -0.5 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.4 | 0 | 3.0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.8 | 3.1 | 3.9 | – | 3.7 | 3.3 | 3.8 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.2259 | 10.8429 | 10.4533 | 8.9453 | 7.9048 | 9.2754 | 12.3626 | 16.3003 | 14.2093 | 13.1616 | 13.3128 | 13.9795 | 14.6582 | 17.644 | 16.1711 | 15.7792 | 16.7003 | 16.0588 |
Actin | 0.0408 | 0.0003 | 0.03 | 0.0488 | 0.0939 | 0.0042 | 0.0161 | 0.0001 | 0.0022 | 0.0346 | 0.0035 | 0.0007 | 0.009 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0077 |
Bud | 0.0008 | 0.0001 | 0.0001 | 0.0006 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.005 | 0.0001 | 0.0003 | 0.0021 | 0.0016 | 0.0029 | 0.0017 | 0.0002 | 0.0006 | 0.0013 | 0.0007 | 0.0012 | 0.0005 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0011 |
Bud Periphery | 0.0011 | 0 | 0.0001 | 0.0004 | 0.0007 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.004 | 0.0001 | 0.0004 | 0.0026 | 0.0055 | 0.0003 | 0.0013 | 0.0002 | 0.0008 | 0.0003 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7506 | 0.988 | 0.9542 | 0.8973 | 0.7643 | 0.9267 | 0.8536 | 0.9827 | 0.9478 | 0.872 | 0.9033 | 0.9449 | 0.9369 | 0.9913 | 0.9658 | 0.9526 | 0.9873 | 0.9587 |
Cytoplasmic Foci | 0.0278 | 0.0006 | 0.0008 | 0.0178 | 0.015 | 0.01 | 0.0135 | 0.0009 | 0.0035 | 0.0131 | 0.0034 | 0.0025 | 0.0057 | 0.0007 | 0.0051 | 0.0087 | 0.0006 | 0.0014 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0105 | 0.0005 | 0.0005 | 0.0025 | 0.0023 | 0.0034 | 0.0079 | 0.0008 | 0.0035 | 0.0032 | 0.0015 | 0.0018 | 0.0022 | 0.0003 | 0.0027 | 0.0012 | 0.0003 | 0.0027 |
Endosome | 0.0273 | 0.0001 | 0.0009 | 0.0027 | 0.0247 | 0.0064 | 0.0246 | 0.0004 | 0.0054 | 0.0155 | 0.0097 | 0.0046 | 0.0039 | 0.0002 | 0.0087 | 0.0071 | 0.0001 | 0.0025 |
Golgi | 0.0126 | 0 | 0.0009 | 0.0028 | 0.0204 | 0.0027 | 0.0025 | 0 | 0.0006 | 0.0067 | 0.0039 | 0.0011 | 0.0016 | 0 | 0.0011 | 0.0002 | 0 | 0.0004 |
Lipid Particles | 0.0231 | 0 | 0.0004 | 0.0008 | 0.0193 | 0.003 | 0.0063 | 0 | 0.0001 | 0.0081 | 0.0204 | 0.0018 | 0.0067 | 0 | 0.0007 | 0.0006 | 0 | 0.0005 |
Mitochondria | 0.0065 | 0.0001 | 0.0003 | 0.0012 | 0.0059 | 0.0005 | 0.003 | 0.0001 | 0.0018 | 0.0017 | 0.0057 | 0.0005 | 0.0024 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0003 |
None | 0.0058 | 0.0011 | 0.0009 | 0.0018 | 0.0014 | 0.001 | 0.0054 | 0.0009 | 0.0017 | 0.0053 | 0.0015 | 0.0012 | 0.0047 | 0.0008 | 0.0009 | 0.0033 | 0.0008 | 0.0009 |
Nuclear Periphery | 0.0277 | 0.0007 | 0.0009 | 0.0024 | 0.0045 | 0.0047 | 0.0148 | 0.0011 | 0.0039 | 0.005 | 0.0034 | 0.0036 | 0.0029 | 0.0004 | 0.0014 | 0.0021 | 0.0005 | 0.0063 |
Nucleolus | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 |
Nucleus | 0.0292 | 0.0079 | 0.0086 | 0.0064 | 0.0124 | 0.0276 | 0.0304 | 0.0115 | 0.0248 | 0.0216 | 0.0332 | 0.0338 | 0.0119 | 0.0054 | 0.0089 | 0.0183 | 0.0097 | 0.0158 |
Peroxisomes | 0.0096 | 0 | 0.0003 | 0.0067 | 0.0226 | 0.0015 | 0.0032 | 0 | 0.0002 | 0.0019 | 0.0042 | 0.0002 | 0.0061 | 0 | 0.0004 | 0.0001 | 0 | 0.0006 |
Punctate Nuclear | 0.0125 | 0.0002 | 0.0003 | 0.0023 | 0.0025 | 0.0037 | 0.0057 | 0.0004 | 0.0011 | 0.0066 | 0.0019 | 0.0015 | 0.0025 | 0.0001 | 0.0006 | 0.0034 | 0.0002 | 0.0006 |
Vacuole | 0.002 | 0.0001 | 0.0001 | 0.0005 | 0.001 | 0.0007 | 0.0062 | 0.0003 | 0.0009 | 0.0009 | 0.0012 | 0.0004 | 0.0004 | 0.0001 | 0.0008 | 0.0011 | 0.0001 | 0.0004 |
Vacuole Periphery | 0.0021 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0017 | 0 | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 165.0621 | 147.5555 | 138.0558 | 149.5027 | 137.2457 | 156.0049 | 150.8148 | 125.8692 | 147.4025 | 131.7283 |
Translational Efficiency | 1.3368 | 1.6685 | 1.5151 | 1.3693 | 1.6672 | 1.6644 | 1.504 | 2.26 | 1.5558 | 1.496 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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