Standard name | |
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Human Ortholog | |
Description | TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 11 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 6 | 7 | 2 | 10 | 1 | 1 | 6 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 6 | 3 | 8 | 16 | 6 | 14 | 30 | 12 | 16 | 5 | 13 | 30 | 84 | 114 | 32 | 16 | 24 | 1 | 1 | 0 | 0 | 2 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 7 | 16 | 7 | 4 | 0 | 0 | 0 | 1 | 1 | 8 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 1 |
Mitochondria | 6 | 11 | 8 | 6 | 8 | 42 | 41 | 102 | 76 | 123 | 55 | 116 | 0 | 2 | 0 | 18 | 3 | 4 | 1 | 1 | 0 | 1 | 4 | 5 |
Nucleus | 232 | 165 | 143 | 151 | 288 | 287 | 440 | 551 | 310 | 513 | 207 | 356 | 612 | 850 | 831 | 320 | 281 | 204 | 222 | 170 | 146 | 509 | 662 | 730 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 10 | 1 | 6 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 44 | 27 | 24 | 17 | 25 | 45 | 86 | 177 | 84 | 216 | 127 | 120 | 54 | 84 | 157 | 18 | 14 | 11 | 7 | 1 | 4 | 23 | 87 | 122 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 11 | 14 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 27 | 32 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 3 |
Vac/Vac Membrane | 0 | 3 | 0 | 1 | 2 | 3 | 2 | 7 | 4 | 0 | 1 | 3 | 1 | 9 | 22 | 8 | 6 | 9 | 4 | 5 | 5 | 15 | 77 | 161 |
Unique Cell Count | 243 | 180 | 153 | 166 | 316 | 321 | 518 | 703 | 388 | 664 | 283 | 470 | 653 | 1003 | 1078 | 384 | 313 | 246 | 243 | 187 | 159 | 559 | 858 | 1064 |
Labelled Cell Count | 287 | 212 | 179 | 186 | 339 | 386 | 588 | 873 | 494 | 886 | 401 | 625 | 706 | 1078 | 1193 | 418 | 325 | 258 | 243 | 187 | 159 | 559 | 858 | 1064 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 9.3 | 8.5 | 9.0 | 8.9 | 7.4 | 7.4 | 6.9 | 7.0 | 6.5 | 6.3 | 6.2 | 13.0 | 12.0 | 11.8 | 10.5 | 13.5 | 14.5 | 11.4 | 10.8 | 11.8 |
Std Deviation (1e-4) | 2.2 | 2.5 | 1.7 | 2.0 | 1.8 | 1.6 | 1.6 | 1.5 | 1.7 | 1.5 | 1.6 | 1.6 | 3.5 | 3.7 | 3.9 | 3.3 | 3.8 | 3.8 | 2.3 | 2.2 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.06 | -0.21 | -0.19 | -0.3 | -0.28 | -0.39 | -0.42 | -0.45 | 0.61 | 0.5 | 0.47 | 0.31 | 0.67 | 0.77 | 0.43 | 0.35 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 1.6 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 1.5 | 2.8 | 3.4 | 2.7 | 1.6 | 3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.7 | -1.5 | 2.6 | 1.1 | 3.1 | 4.2 | 4.0 | 4.0 | 5.2 | 0 | 0 | 0 | -0.3 | 0 | 0 |
Nucleus | -0.8 | -0.9 | -1.4 | -2.7 | -4.3 | -3.9 | -4.5 | -5.1 | -4.8 | 0.1 | -2.9 | -4.7 | -3.1 | -1.3 | -3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.5 | -2.6 | -0.5 | 0.3 | 2.5 | 1.6 | 4.1 | 6.1 | 2.5 | -2.8 | -2.9 | -0.4 | -4.3 | -4.2 | -3.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.7793 | 12.4744 | 12.1278 | 11.6448 | 11.2601 | 11.8943 | 6.184 | 6.0409 | 5.9775 | 6.2954 | 3.1755 | 6.1234 | 7.213 | 7.1756 | 6.7525 | 7.6457 | 7.4812 | 7.926 |
Actin | 0.0005 | 0 | 0.0014 | 0.0001 | 0.0019 | 0.0003 | 0.005 | 0 | 0.0194 | 0 | 0.0908 | 0.015 | 0.007 | 0 | 0.0238 | 0 | 0 | 0 |
Bud | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0011 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0.0005 | 0 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0004 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0012 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0004 | 0 | 0.0014 | 0 | 0.0006 | 0.0001 | 0.0017 | 0.0002 | 0.0011 | 0 | 0.0028 | 0.0001 | 0.0008 | 0.0001 | 0.0016 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Cytoplasm | 0.003 | 0 | 0.0016 | 0.0001 | 0.0006 | 0.0003 | 0.0079 | 0.0247 | 0.0008 | 0 | 0.0052 | 0 | 0.0028 | 0.0072 | 0.0004 | 0.0001 | 0 | 0 |
Cytoplasmic Foci | 0.0043 | 0 | 0.0008 | 0.0028 | 0.0079 | 0.0001 | 0.0361 | 0.0002 | 0.0089 | 0 | 0.0296 | 0.0004 | 0.0072 | 0.0001 | 0.0108 | 0 | 0 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0004 | 0 | 0.0019 | 0.0001 | 0.0002 | 0.0002 | 0.0018 | 0.0002 | 0.0002 | 0 | 0.0035 | 0.0001 | 0.005 | 0.0002 | 0.0007 | 0 | 0 | 0 |
Endosome | 0.0012 | 0 | 0.0034 | 0.0005 | 0.0066 | 0.0001 | 0.0074 | 0.0019 | 0.0078 | 0 | 0.045 | 0.0003 | 0.015 | 0.0005 | 0.0036 | 0 | 0 | 0 |
Golgi | 0.0003 | 0 | 0.0007 | 0.0003 | 0.0049 | 0 | 0.0029 | 0 | 0.003 | 0 | 0.0243 | 0.0006 | 0.0017 | 0 | 0.0013 | 0 | 0 | 0 |
Lipid Particles | 0.0038 | 0 | 0.0006 | 0.0041 | 0.0064 | 0.0001 | 0.022 | 0 | 0.0027 | 0 | 0.0113 | 0.0007 | 0.0026 | 0 | 0.0041 | 0 | 0 | 0 |
Mitochondria | 0.0019 | 0.0001 | 0.0007 | 0 | 0.0023 | 0.0005 | 0.0032 | 0.0001 | 0.0006 | 0.0002 | 0.0093 | 0.0001 | 0.0017 | 0.0002 | 0.0099 | 0 | 0.0003 | 0.0002 |
None | 0.0011 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0032 | 0.0003 | 0.0209 | 0 | 0.0033 | 0.0001 | 0.0018 | 0.0001 | 0.0006 | 0 | 0 | 0 |
Nuclear Periphery | 0.0032 | 0.0015 | 0.0031 | 0.0035 | 0.0029 | 0.0008 | 0.0236 | 0.0036 | 0.0013 | 0.0016 | 0.0054 | 0.0003 | 0.0142 | 0.0051 | 0.0065 | 0.0031 | 0.0006 | 0.0001 |
Nucleolus | 0.0456 | 0.0162 | 0.0136 | 0.0077 | 0.1068 | 0.0779 | 0.0188 | 0.0111 | 0.0052 | 0.0626 | 0.0609 | 0.0485 | 0.0536 | 0.0199 | 0.0186 | 0.0052 | 0.0779 | 0.0502 |
Nucleus | 0.9247 | 0.9809 | 0.9659 | 0.9528 | 0.8469 | 0.9175 | 0.8018 | 0.9418 | 0.9239 | 0.9348 | 0.6874 | 0.9323 | 0.8424 | 0.9576 | 0.9046 | 0.9908 | 0.9201 | 0.9486 |
Peroxisomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.005 | 0 | 0.0176 | 0 | 0.0028 | 0 | 0.0117 | 0.0005 | 0.0031 | 0 | 0.0052 | 0 | 0 | 0 |
Punctate Nuclear | 0.0055 | 0.0011 | 0.0027 | 0.0278 | 0.0054 | 0.0009 | 0.0215 | 0.0007 | 0.0006 | 0.0003 | 0.0045 | 0.0004 | 0.0333 | 0.0014 | 0.0031 | 0.0001 | 0.0004 | 0.0001 |
Vacuole | 0.0017 | 0 | 0.0008 | 0 | 0.0004 | 0.0001 | 0.0132 | 0.0138 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0031 | 0.0063 | 0.0016 | 0.0003 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0013 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0097 | 0.0012 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0015 | 0.0011 | 0.0022 | 0.0002 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.3635 | 40.163 | 36.8745 | 38.1747 | 36.5305 | 31.965 | 33.7259 | 41.3445 | 47.6491 | 35.0598 |
Translational Efficiency | 0.8855 | 0.8267 | 0.7565 | 1.0403 | 0.7643 | 0.873 | 0.8133 | 0.6865 | 0.5929 | 0.6961 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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