Standard name
Human Ortholog
Description Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.09 0.09 0.07 0.07 0.06 0.06 0 – 0 0.05 0.06 0.13 0.14 0.2 0.26 0.17 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0.72 0.75 0.37 0.76 0.71 0.75 0.88 – 0.78 0.85 0 0 0.2 0.08 0.09 0.08 0 0 0 0 0 0
Nucleus 0.9 0.81 0.54 0.61 0.73 0.58 0.69 0.64 0.59 – 0.69 0.65 0.87 0.78 0.73 0.59 0.61 0.71 0.8 0.84 0.57 0.76 0.68 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.07 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0.2 0.13 0.12 0.11 0.08 0.26 0.15 0.17 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 1 0 0 2 4 9 3 – 8 6 0 1 1 0 0 0 5 0 2 1 5 5
Bud Neck 0 0 0 0 1 2 0 1 0 – 1 1 0 0 0 0 0 0 1 0 0 0 1 2
Bud Site 0 0 0 0 1 2 13 15 9 – 6 15 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 4 12 19 27 24 30 41 9 – 13 20 10 30 34 42 37 52 7 1 7 3 10 9
Endoplasmic Reticulum 1 1 0 1 0 1 0 0 0 – 0 0 0 0 3 11 7 20 0 0 1 0 0 1
Endosome 3 0 0 0 5 0 0 0 0 – 0 0 1 2 0 2 4 4 4 1 1 0 1 1
Golgi 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 0 1 0
Mitochondria 13 46 91 164 148 260 369 506 266 – 239 360 7 10 47 16 12 24 4 2 5 0 1 3
Nucleus 357 122 68 133 294 201 357 431 177 – 211 274 136 174 177 124 86 217 356 137 84 70 167 161
Nuclear Periphery 0 0 1 2 1 0 1 2 2 – 1 2 0 0 0 0 0 0 2 0 0 0 0 2
Nucleolus 0 0 0 1 1 0 1 6 1 – 1 7 0 0 2 1 0 0 0 1 0 0 4 11
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 2 3 1 5 6 2 – 1 0 0 0 0 2 0 2 6 1 3 0 5 7
Vac/Vac Membrane 28 11 2 3 16 6 12 5 3 – 4 6 7 13 13 43 18 37 50 13 39 13 43 45
Unique Cell Count 395 151 127 218 403 344 520 676 302 307 424 157 223 241 211 140 304 445 163 149 93 247 257
Labelled Cell Count 413 184 176 325 497 500 792 1022 472 485 691 161 230 277 242 164 356 445 163 149 93 247 257


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.2 3.3 3.2 3.7 3.2 3.2 3.2 2.8 – 3.1 2.9 5.2 5.2 4.6 5.5 6.1 6.6 4.4 4.7 4.9
Std Deviation (1e-4) 0.6 0.6 0.7 0.8 0.9 0.7 1.0 1.0 0.6 – 0.9 1.0 1.4 1.5 1.5 1.3 1.9 1.9 0.8 1.3 1.1
Intensity Change (Log2) – – – -0.02 0.15 -0.04 -0.02 -0.02 -0.21 – -0.1 -0.2 0.67 0.66 0.47 0.74 0.89 1.0 0.42 0.5 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.9 3.5 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5089 3.145 2.6394 2.4716 2.0498 2.901 2.452 1.8506 1.5523 1.3273 1.3448 1.3635 0.4918 -0.1143 -0.0823 0.2224 0.2929 -0.1848
Actin 0.0002 0.0001 0 0 0 0 0.0848 0.0006 0.0089 0.0188 0.0134 0.0003 0.0002 0.0001 0.0001 0 0.0003 0
Bud 0 0 0 0 0 0 0.0011 0.0001 0.0004 0.001 0.0005 0.0003 0 0 0.0001 0 0 0
Bud Neck 0.0002 0.0002 0.0002 0.0001 0.0007 0.0042 0.0018 0.0002 0.0019 0.0003 0.0007 0.0014 0.0002 0.0001 0.0003 0.0001 0.0006 0.001
Bud Periphery 0 0 0 0 0 0.0001 0.0019 0.0001 0.0005 0.0016 0.0003 0.0004 0 0 0.0001 0 0 0
Bud Site 0.0002 0.0004 0.0009 0 0.0001 0.0001 0.0027 0.001 0.003 0.0044 0.0014 0.0003 0.0003 0.0001 0.0004 0 0.0001 0
Cell Periphery 0 0 0 0 0 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0 0 0 0.0001 0 0 0
Cytoplasm 0.0136 0.0171 0.004 0.0005 0.0007 0.0009 0.0295 0.0234 0.0057 0.0004 0.0058 0.0012 0.0197 0.0711 0.0102 0.0205 0.0109 0.0055
Cytoplasmic Foci 0.0017 0.0003 0 0 0 0 0.0321 0.0008 0.0057 0.0125 0.0125 0.0002 0.008 0.0014 0 0.0003 0.0004 0.0002
Eisosomes 0 0 0 0 0 0 0.0006 0 0 0.0002 0 0 0 0 0 0 0.0006 0
Endoplasmic Reticulum 0.0023 0.0041 0.0002 0 0 0.0001 0.0045 0.0014 0.0015 0.0004 0.0006 0.0003 0.0005 0.0033 0.0007 0.0002 0.002 0.0003
Endosome 0.0041 0.002 0.0002 0 0 0.0001 0.0344 0.0052 0.0066 0.0136 0.0594 0.0018 0.0025 0.0014 0.0003 0.0001 0.0022 0.0004
Golgi 0.0001 0 0 0 0 0 0.012 0.0011 0.0039 0.0046 0.0254 0.0002 0.0001 0.0001 0 0 0 0
Lipid Particles 0.0004 0.0001 0 0 0 0 0.0253 0.0001 0.0044 0.0047 0.0023 0.0001 0.0004 0.0003 0 0 0.0003 0
Mitochondria 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0113 0.0015 0.0023 0.0171 0.0027 0.0018 0.0002 0.0002 0.0003 0.0001 0.0004 0.0001
None 0.0107 0.003 0.0003 0.0001 0.0001 0.0002 0.0288 0.0117 0.011 0.0002 0.0004 0.0002 0.0094 0.1204 0.0379 0.095 0.1457 0.0322
Nuclear Periphery 0.0091 0.0047 0.0034 0.0014 0.0006 0.0015 0.0426 0.0055 0.01 0.0011 0.0029 0.0038 0.0103 0.0082 0.0127 0.002 0.089 0.0079
Nucleolus 0.0053 0.0052 0.0041 0.0026 0.0087 0.0146 0.0174 0.0059 0.0043 0.0019 0.005 0.0092 0.0041 0.0036 0.0044 0.0021 0.0091 0.008
Nucleus 0.9393 0.9572 0.9846 0.9946 0.9877 0.9745 0.575 0.9331 0.915 0.861 0.8596 0.975 0.7954 0.7727 0.9285 0.8708 0.6827 0.9319
Peroxisomes 0 0 0 0 0 0 0.0231 0 0.0092 0.0253 0.0015 0.0001 0.0001 0 0 0 0 0
Punctate Nuclear 0.0107 0.0042 0.0013 0.0006 0.001 0.0028 0.0641 0.0063 0.0031 0.0004 0.0021 0.0021 0.1481 0.0161 0.0026 0.0087 0.054 0.012
Vacuole 0.0013 0.0011 0.0005 0 0.0001 0.0005 0.0034 0.0014 0.0019 0.0174 0.0022 0.0008 0.0004 0.0008 0.0013 0.0001 0.0006 0.0003
Vacuole Periphery 0.0004 0.0001 0.0001 0 0 0.0001 0.0036 0.0004 0.0008 0.0128 0.0013 0.0004 0.0001 0.0001 0.0002 0 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.3073 8.7041 11.876 19.4425 15.8821 13.8124 19.5178 20.5023 18.4535 20.1219
Translational Efficiency 0.5077 0.4972 0.4086 0.2906 0.3509 0.4497 0.3272 0.3127 0.3399 0.3218

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (45%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rgt1

Rgt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available