Standard name
Human Ortholog
Description Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.51 0.39 0.37 0.52 0.75 0.73 0.61 0.64 0.34 0.41 0.26 0.31 0.23 0.67 0.93 0.86 0.73 0.73 0.33 0.3 0.22 0.11 0.46 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0 0 0 0.05 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0 0.05 0.06 0.17 0.36 0.32 0.56 0.47 0.68 0.52 0 0 0 0 0 0.05 0.06 0 0.07 0 0 0
Nucleus 0.18 0.27 0.47 0.27 0.06 0.07 0.08 0.09 0.2 0.19 0.21 0.21 0.5 0 0 0 0.06 0.06 0.22 0.35 0.38 0.52 0.16 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.05 0.11 0.08 0.1
Vac/Vac Membrane 0.16 0.34 0.16 0.19 0.16 0.11 0.11 0.06 0.09 0.1 0.1 0.13 0.22 0.35 0.12 0.1 0.19 0.19 0.13 0.12 0.14 0.11 0.13 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 3 2 2 0 3 5
Bud 0 0 0 0 0 4 7 3 14 14 16 10 0 0 1 0 0 0 11 5 4 15 12 20
Bud Neck 0 0 1 0 2 1 2 5 3 5 0 4 0 0 0 0 0 0 3 3 1 3 8 10
Bud Site 0 0 0 0 0 0 2 1 5 5 10 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 1 2 0 0 0 0 0 0 1 2 0 4 0 0 0 0 0 1
Cytoplasm 175 81 55 140 283 279 311 298 172 213 109 150 61 227 455 214 92 154 146 79 43 34 190 298
Endoplasmic Reticulum 1 3 1 1 1 2 3 2 0 0 0 0 0 0 0 20 4 14 1 1 1 2 1 4
Endosome 7 9 4 12 13 7 4 4 8 6 3 6 27 32 7 3 4 10 22 8 4 2 10 13
Golgi 1 0 0 1 1 0 0 0 0 0 0 0 0 0 2 1 1 3 2 2 0 1 1 0
Mitochondria 81 5 1 14 22 65 185 151 286 241 284 251 1 2 0 7 4 10 27 4 13 2 11 8
Nucleus 63 55 70 73 23 25 42 44 99 99 90 103 132 8 2 7 7 12 96 93 75 163 67 35
Nuclear Periphery 0 1 0 1 1 1 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 1 1 0 3 1 0 0 1 1 0 0 6 8 5 15 6 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 2 0 1 2 1 1 5 5 8 6 4 4 4 0 0 1 0 1 36 17 9 33 34 49
Vac/Vac Membrane 55 70 23 52 61 41 54 29 47 51 41 63 59 119 57 25 24 40 57 32 27 34 52 32
Unique Cell Count 342 207 148 268 376 383 512 468 507 517 419 486 264 337 491 250 126 211 437 263 199 316 412 499
Labelled Cell Count 386 225 156 296 408 426 616 545 643 640 561 598 284 388 527 282 137 249 437 263 199 316 412 499


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.0 6.0 5.8 5.4 4.5 4.1 4.5 4.0 4.0 3.7 4.0 6.8 6.5 6.1 5.1 6.0 5.8 6.2 6.1 6.3
Std Deviation (1e-4) 0.8 0.8 1.2 2.0 1.7 1.6 1.6 1.6 1.6 1.1 1.2 0.9 2.2 1.8 1.8 1.1 1.2 1.5 1.6 1.8 1.8
Intensity Change (Log2) – – – -0.05 -0.14 -0.39 -0.53 -0.4 -0.57 -0.57 -0.68 -0.57 0.18 0.13 0.03 -0.22 0.0 -0.04 0.05 0.04 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 8.2 7.6 5.1 5.7 -0.7 0.9 -2.6 -1.4 -3.0 6.2 14.7 10.0 5.9 6.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 0 2.8
Endosome 0.9 0 0 0 0 0 0 0 0 2.8 2.6 0 0 0 1.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -4.1 -11.1 -11.0 -11.2 -10.3 -6.8 -6.9 -6.0 -6.2 0.5 -12.4 -15.8 -10.9 -7.7 -9.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 0.2 -1.5 -1.7 -3.6 -2.2 -1.9 -1.9 -0.8 1.7 4.4 -1.3 -1.6 0.8 0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0103 0.0012 0.0119 0.0077 0.0078 0.002 0.0243 0.0072 0.0058 0.0003 0.0402 0.0009 0.0563 0.0003 0.0295 0.0135 0.0001 0.0003
Bud 0.0008 0.0009 0.0037 0.0013 0.0004 0.0004 0.0005 0.0005 0.0022 0.0003 0.0003 0.0014 0.0012 0.0006 0.0005 0.0179 0.0001 0.0006
Bud Neck 0.0021 0.0009 0.0022 0.0054 0.0015 0.0032 0.0018 0.0007 0.0017 0.0018 0.0015 0.0035 0.0016 0.0005 0.0011 0.0053 0.0009 0.0035
Bud Periphery 0.0012 0.0006 0.0012 0.0018 0.0008 0.0004 0.0009 0.0003 0.0013 0.0003 0.0003 0.0012 0.0017 0.0002 0.0003 0.0248 0 0.0003
Bud Site 0.0075 0.006 0.0041 0.0009 0.0009 0.0005 0.0065 0.0041 0.0038 0.0006 0.0009 0.0007 0.007 0.001 0.0005 0.0436 0.0001 0.0006
Cell Periphery 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0005 0.0001 0.0001 0 0.0001 0.0002 0.0006 0.0001 0.0001 0.0014 0 0
Cytoplasm 0.1966 0.3315 0.2397 0.3035 0.2806 0.1788 0.1197 0.1929 0.1459 0.1318 0.0529 0.1429 0.0607 0.2019 0.1528 0.0625 0.004 0.1681
Cytoplasmic Foci 0.0167 0.0063 0.0145 0.0139 0.0099 0.0071 0.0145 0.0018 0.0063 0.0119 0.0151 0.003 0.0644 0.0009 0.0009 0.0232 0 0.0004
Eisosomes 0.0001 0 0.0002 0 0.0001 0 0.0006 0 0.0001 0 0.0001 0 0.0007 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0033 0.0026 0.0053 0.0027 0.002 0.0017 0.0056 0.0022 0.0021 0.0034 0.0011 0.004 0.0048 0.0022 0.0019 0.0075 0.0001 0.0013
Endosome 0.0258 0.0045 0.0151 0.0055 0.0187 0.0062 0.028 0.0018 0.0163 0.0407 0.0089 0.0085 0.0712 0.0012 0.0013 0.0662 0.0001 0.0006
Golgi 0.0054 0.0004 0.0055 0.0029 0.0083 0.0012 0.0142 0.0001 0.0046 0.0266 0.0374 0.0021 0.0302 0 0.0032 0.0361 0 0
Lipid Particles 0.0086 0.0003 0.0061 0.0106 0.029 0.0007 0.0068 0.0001 0.0057 0.0183 0.0072 0.0004 0.024 0 0.0001 0.0073 0 0
Mitochondria 0.0075 0.0012 0.0087 0.001 0.0087 0.0036 0.0186 0.0004 0.0224 0.0008 0.0053 0.0241 0.0433 0.0003 0.0022 0.0856 0.0002 0.0003
None 0.0561 0.0622 0.0826 0.0903 0.0198 0.0377 0.0134 0.035 0.066 0.037 0.0007 0.0127 0.0015 0.0023 0.0293 0.0017 0 0.0074
Nuclear Periphery 0.0174 0.0109 0.0142 0.0128 0.014 0.0091 0.0202 0.0124 0.0105 0.0251 0.0111 0.0078 0.006 0.0127 0.0115 0.013 0.0071 0.0132
Nucleolus 0.0026 0.002 0.0023 0.0019 0.0018 0.0039 0.0032 0.0023 0.0019 0.004 0.0018 0.0026 0.0018 0.0014 0.0027 0.0021 0.0015 0.0027
Nucleus 0.5811 0.5494 0.5551 0.484 0.5586 0.7012 0.6947 0.7276 0.6737 0.6017 0.7809 0.7559 0.5687 0.7677 0.7491 0.5508 0.9843 0.7907
Peroxisomes 0.0233 0.0019 0.0061 0.0177 0.0212 0.0037 0.0015 0.0001 0.006 0.0004 0.0274 0.0009 0.0435 0 0.0002 0.0067 0 0
Punctate Nuclear 0.0257 0.0147 0.0187 0.0347 0.013 0.0369 0.0181 0.0082 0.0213 0.0913 0.0023 0.0215 0.003 0.0053 0.0116 0.0065 0.0012 0.0088
Vacuole 0.0057 0.0021 0.0021 0.0012 0.0018 0.0014 0.0052 0.0018 0.0014 0.0025 0.0043 0.0045 0.0054 0.001 0.001 0.0202 0.0002 0.0009
Vacuole Periphery 0.0019 0.0004 0.0005 0.0003 0.0011 0.0003 0.0013 0.0003 0.0007 0.0011 0.0005 0.001 0.0026 0.0002 0.0002 0.0038 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0328 10.7507 7.3127 16.792 10.395 20.0326 12.4552 14.6731 21.0628 14.0129
Translational Efficiency 0.4954 0.7481 1.0238 0.5151 0.8085 0.5655 0.5633 0.702 0.6593 0.667

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (69%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Phd1

Phd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Phd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available