Standard name
Human Ortholog
Description Plasma membrane protein that may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.17 0.16 0.39 0.26 0.39 0.28 0.18 0.12 0.18 0.12 0.14 0.12 0.26 0.24 0.14 0.23 0.2 0.21 0 0 0 0 0 0
Bud 0 0 0 0 0.1 0.12 0.09 0.11 0.13 0.1 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.87 0.91 0.88 0.88 0.67 0.47 0.31 0.27 0.35 0.26 0.37 0.29 0.92 0.91 0.94 0.83 0.82 0.75 0.86 0.89 0.93 0.9 0.88 0.88
Cytoplasm 0.1 0.06 0.07 0.1 0.2 0.38 0.39 0.46 0.44 0.46 0.4 0.44 0.08 0.07 0 0.16 0.18 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0.21 0.23 0.19 0.21 0.36 0.27 0.38 0.35 0.5 0.44 0 0 0 0.08 0.06 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 27 30 70 72 122 114 113 62 74 69 58 64 44 93 52 25 26 21 5 6 2 7 4 5
Bud 1 1 5 6 30 49 56 57 52 59 42 56 1 3 1 2 1 1 2 1 1 1 3 3
Bud Neck 0 0 0 0 0 0 1 2 1 0 0 1 0 0 0 0 0 0 1 0 0 1 5 3
Bud Site 0 0 1 0 2 12 5 6 6 6 5 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 135 174 158 245 211 190 194 146 141 150 153 154 158 349 339 89 107 75 159 197 181 244 241 258
Cytoplasm 16 12 12 27 63 153 242 243 179 261 165 236 13 28 14 17 23 17 5 1 1 5 5 5
Endoplasmic Reticulum 2 2 2 8 5 6 18 3 3 9 1 3 2 11 10 3 2 6 1 6 2 4 6 5
Endosome 0 0 0 0 0 0 2 1 0 2 0 0 0 0 1 1 1 1 1 0 0 0 0 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Mitochondria 11 9 37 65 59 87 223 145 153 200 206 233 1 5 6 9 8 10 1 2 3 5 3 2
Nucleus 1 0 1 0 2 1 4 14 6 7 4 6 1 0 2 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 3 1 3 14 6 8 3 5 3 0 0 1 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 3 2 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 3 3 1 7 10 3 6 5 9 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 7 21 30 33 11 19 4 9 0 0 1 0 2 0 1 0 0 0 0 0
Vac/Vac Membrane 0 0 0 3 8 1 7 27 5 17 5 1 0 0 0 0 0 3 0 2 0 1 1 4
Unique Cell Count 155 191 179 277 315 406 627 532 407 570 409 533 172 385 361 107 131 100 187 222 195 273 274 295
Labelled Cell Count 193 228 287 433 513 638 916 758 644 810 654 782 220 490 428 146 171 135 187 222 195 273 274 295


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.6 18.2 13.3 13.7 10.3 7.7 6.8 6.9 6.2 5.9 5.5 5.6 17.1 17.4 17.9 12.5 13.1 12.8 15.7 15.4 15.2
Std Deviation (1e-4) 4.5 4.4 3.2 3.3 3.3 3.1 2.8 2.7 2.8 2.6 2.4 2.4 3.7 4.2 4.4 2.9 3.2 3.4 3.6 4.0 3.7
Intensity Change (Log2) – – – 0.05 -0.36 -0.78 -0.96 -0.95 -1.1 -1.18 -1.27 -1.26 0.37 0.39 0.44 -0.08 -0.02 -0.05 0.24 0.22 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.0 -0.1 -2.6 -5.9 -8.2 -5.4 -8.1 -6.7 -8.0 -2.7 -3.6 -6.5 -2.7 -3.6 -3.1
Bud 0 2.8 3.6 2.7 3.2 3.8 3.2 3.1 3.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -5.2 -9.4 -13.6 -14.2 -12.0 -14.7 -11.4 -13.9 1.1 0.9 2.3 -1.2 -1.6 -2.9
Cytoplasm 1.1 4.0 7.7 8.1 9.4 8.9 9.5 8.2 9.1 0.3 0.2 -1.4 2.5 3.0 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 -0.5 0.2 3.8 1.7 4.0 3.6 6.7 5.5 -6.1 -8.2 -7.7 -2.7 -3.6 -2.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2.7 2.6 3.1 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.4053 15.234 13.3377 13.1513 10.1102 12.9226 14.1899 20.2615 16.9513 15.3441 15.165 17.4681 16.3528 22.6027 19.0972 19.3087 18.812 18.8417
Actin 0.0052 0.0004 0.0077 0.0006 0.0098 0.003 0.0165 0 0.0139 0.0175 0.0103 0.0087 0.0004 0 0 0 0 0
Bud 0.0009 0.0007 0.0004 0.0001 0.0013 0.0001 0.0008 0 0.0013 0.0018 0.0083 0.0002 0.0001 0 0 0 0.0001 0
Bud Neck 0.0004 0.0001 0.0003 0.0001 0.0004 0.0001 0.0006 0 0.0005 0.0075 0.0005 0.0008 0.0002 0.0001 0.0001 0.0003 0.0002 0
Bud Periphery 0.0085 0.0028 0.0043 0.002 0.0107 0.0017 0.0099 0.0006 0.0069 0.008 0.0333 0.0028 0.0021 0.0004 0.0009 0.0018 0.0016 0.0013
Bud Site 0.0022 0.0043 0.0018 0.0004 0.0022 0.0003 0.0042 0.0008 0.0074 0.0075 0.0173 0.0003 0.001 0.0009 0.0002 0 0.0001 0
Cell Periphery 0.8724 0.9572 0.9345 0.914 0.8033 0.9611 0.8758 0.9674 0.9136 0.8116 0.869 0.9648 0.9494 0.9723 0.9705 0.9334 0.9713 0.9739
Cytoplasm 0.0041 0.0094 0.0042 0.0022 0.0028 0.0016 0.0099 0.0088 0.0058 0.0349 0.0044 0.0018 0.0055 0.0046 0.0034 0.0024 0.0029 0.0008
Cytoplasmic Foci 0.0038 0 0.0009 0.0024 0.0074 0.0015 0.0114 0 0.001 0.0141 0.007 0.0001 0 0 0 0 0 0
Eisosomes 0.0099 0.0036 0.0047 0.0072 0.0057 0.0014 0.0035 0.0005 0.006 0.0017 0.0013 0.0004 0.0019 0.0001 0 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0109 0.0089 0.0121 0.0203 0.0298 0.0071 0.0125 0.0041 0.0122 0.0246 0.0083 0.0075 0.0067 0.0037 0.006 0.033 0.0051 0.0071
Endosome 0.0027 0 0.0019 0.0218 0.0063 0.0012 0.0102 0 0.0029 0.0152 0.0054 0.0002 0.0001 0 0 0 0 0
Golgi 0.0017 0 0.0011 0.004 0.0056 0.0015 0.0052 0 0.0014 0.0078 0.0032 0.0004 0.0001 0 0 0 0 0
Lipid Particles 0.0049 0.0001 0.0014 0.0044 0.0375 0.0009 0.0051 0.0001 0.0018 0.0056 0.0017 0.0003 0.0003 0.0002 0.0001 0.0003 0.0001 0
Mitochondria 0.032 0.0005 0.0108 0.0082 0.0305 0.0083 0.0059 0.0002 0.008 0.0057 0.0079 0.0001 0.0008 0 0 0 0 0.0007
None 0.0068 0.0036 0.0013 0.0011 0.011 0.0005 0.0009 0 0.0008 0.0018 0.0005 0.0001 0.0002 0 0 0 0 0
Nuclear Periphery 0.0056 0.0007 0.0018 0.0014 0.0011 0.0006 0.0026 0.0014 0.0011 0.0053 0.0013 0.0007 0.0021 0.0012 0.0007 0.003 0.0007 0.0004
Nucleolus 0.0004 0.0001 0.0003 0.0001 0.0007 0.0021 0.0003 0 0.0003 0.0023 0.0003 0 0.0001 0 0 0 0 0
Nucleus 0.0007 0.0002 0.0004 0.0002 0.0003 0.0003 0.0003 0.0001 0.0006 0.0082 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Peroxisomes 0.0076 0 0.0003 0.0004 0.0232 0.0001 0.0036 0 0.0003 0.0015 0.0012 0 0 0 0 0 0 0
Punctate Nuclear 0.0004 0.0001 0.0007 0.0001 0.0005 0.0001 0.0007 0 0.0001 0.0012 0.001 0 0 0 0 0 0 0
Vacuole 0.0169 0.0073 0.0086 0.0083 0.0092 0.0062 0.0196 0.0159 0.0136 0.0142 0.0164 0.0104 0.0288 0.0163 0.0179 0.025 0.0175 0.0154
Vacuole Periphery 0.0018 0.0001 0.0005 0.0007 0.0008 0.0002 0.0005 0.0001 0.0006 0.002 0.0007 0.0001 0.0003 0.0001 0.0001 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5848 15.7894 6.892 10.7812 4.7148 16.6146 10.5649 11.092 9.2639 9.8323
Translational Efficiency 0.9639 0.8722 1.1882 0.657 1.4346 0.9216 1.3094 1.2076 1.1459 0.7937

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane protein that may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane
Localization
Cell Percentages cell periphery (100%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Sfk1

Sfk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available