Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein; forms complex with Ups2p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.85 0.86 0.85 0.85 0.7 0.57 0.46 0.42 0.38 0.36 0.37 0.35 0.97 0.96 0.95 0.88 0.74 0.86 0.81 0.74 0.64 0.74 0.71 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.09 0 0 0 0 0 0
Endosome 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0 0.06 0.06 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.29 0.25 0.58 0.65 0.67 0.77 0.73 0.72 0.79 0 0 0 0.05 0 0 0 0.06 0.15 0.06 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0 0 0.09 0.07 0.06 0 0 0 0 0 0 0 0.05 0.06 0.12 0.09 0 0 0.05 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 2 1 0 0 1 0 0 0 0 0 0 0 1 0 2 0 5 7 6 4 6 7
Bud 6 3 11 3 4 11 9 11 12 9 19 12 0 0 0 0 1 0 4 6 2 3 7 10
Bud Neck 0 0 4 0 0 0 0 0 2 2 0 0 0 0 0 0 1 0 4 5 3 4 7 12
Bud Site 0 0 0 0 0 1 1 4 0 3 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 8 1 2 5 0 1 2 2 2 4 3 2 3 3 5 3 4 0 1 2 1 2 3
Cytoplasm 219 261 270 209 198 207 208 170 169 166 122 135 409 411 511 98 51 64 242 260 230 202 281 283
Endoplasmic Reticulum 1 13 7 1 2 3 2 4 4 18 3 8 5 6 7 11 1 7 5 9 10 6 14 13
Endosome 14 5 0 4 19 7 5 7 0 0 0 0 4 10 3 2 5 1 8 16 12 16 23 44
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 3 0 1 3 1 0 2 3
Mitochondria 9 1 46 71 70 209 297 268 345 341 238 300 0 1 1 6 3 2 5 20 54 15 17 27
Nucleus 5 0 1 0 2 2 9 6 2 3 2 3 1 2 0 5 2 1 7 0 0 2 1 1
Nuclear Periphery 1 0 0 0 2 0 2 1 1 1 0 1 0 0 0 0 0 0 1 1 1 0 1 2
Nucleolus 0 0 0 0 1 0 1 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 1 0 0
SpindlePole 1 0 0 0 0 1 4 6 1 4 4 5 0 0 2 0 0 1 4 3 9 2 6 10
Vac/Vac Membrane 16 21 7 6 24 27 27 18 15 18 13 12 17 10 27 7 8 7 5 8 17 9 20 22
Unique Cell Count 259 303 318 247 282 363 456 402 446 467 332 381 423 428 540 112 69 74 301 354 362 274 398 450
Labelled Cell Count 279 315 349 297 327 468 567 499 553 567 408 484 438 445 556 136 80 87 301 354 362 274 398 450


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.2 4.3 4.9 5.0 3.7 3.8 3.7 3.2 3.5 3.3 3.3 8.1 7.8 7.1 8.0 8.7 7.9 7.6 7.9 7.7
Std Deviation (1e-4) 4.6 1.2 0.8 1.4 1.5 1.0 1.5 0.8 0.8 0.5 0.9 0.5 1.2 1.2 1.2 2.3 2.4 2.1 1.3 1.3 1.3
Intensity Change (Log2) – – – 0.19 0.22 -0.23 -0.16 -0.2 -0.43 -0.31 -0.38 -0.4 0.91 0.87 0.73 0.9 1.02 0.88 0.83 0.88 0.86


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -4.3 -7.9 -11.1 -11.6 -13.0 -13.7 -12.5 -13.2 5.7 5.3 4.8 0.7 -2.2 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -1.1 -0.8 -1.0 0 0 0
Endosome 0 4.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.2 0 0 0 0 0 0 0 -8.1 -7.9 -8.9 -2.5 -2.3 -2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 3.5 3.1 2.5 1.7 0.9 1.3 1.3 0.8 1.4 0.1 2.0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8502 4.6022 4.1866 4.0583 3.3183 4.186 2.4251 3.6638 3.3493 2.9426 3.3391 3.1142 4.9762 5.1349 4.7422 5.1363 4.259 4.9475
Actin 0.0422 0.0032 0.0042 0.0064 0.0058 0.0034 0.0254 0.0021 0.0106 0.0005 0.0628 0.011 0.0048 0.0006 0.0023 0.0007 0.0009 0.0005
Bud 0.0007 0.0017 0.0026 0.0019 0.0035 0.0005 0.0017 0.001 0.0017 0.0002 0.0016 0.0057 0.0005 0.0024 0.0025 0.0002 0.0002 0.0002
Bud Neck 0.0168 0.0009 0.0158 0.0012 0.0115 0.0024 0.0154 0.0002 0.0019 0.0009 0.0026 0.0048 0.0022 0.001 0.0024 0.0008 0.0008 0.004
Bud Periphery 0.0009 0.0008 0.0014 0.0027 0.0026 0.0007 0.0022 0.0004 0.0011 0.0002 0.0014 0.0048 0.0006 0.0014 0.0014 0.0001 0.0001 0.0001
Bud Site 0.0076 0.0065 0.0127 0.0376 0.0056 0.001 0.0096 0.0045 0.0048 0.0006 0.0206 0.0017 0.0022 0.0128 0.0092 0.0002 0.0003 0.0003
Cell Periphery 0.0004 0.0002 0.0004 0.0003 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0003 0.0002 0 0 0
Cytoplasm 0.6379 0.9079 0.7869 0.8657 0.6604 0.8918 0.6399 0.9102 0.8578 0.8135 0.7675 0.8604 0.8389 0.9094 0.8338 0.8572 0.9306 0.8878
Cytoplasmic Foci 0.0515 0.0146 0.0347 0.0125 0.076 0.024 0.0496 0.0046 0.0169 0.0101 0.0144 0.0154 0.0087 0.009 0.0109 0.041 0.0186 0.0159
Eisosomes 0.0004 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0197 0.0057 0.0079 0.0086 0.0064 0.0055 0.0069 0.0036 0.0068 0.0041 0.0078 0.0072 0.0108 0.0033 0.0058 0.0136 0.0048 0.0065
Endosome 0.0407 0.008 0.0301 0.0193 0.0631 0.0179 0.0348 0.002 0.0097 0.052 0.0181 0.0169 0.0093 0.0059 0.0086 0.0244 0.0233 0.0117
Golgi 0.0129 0.0007 0.0056 0.0045 0.0278 0.0029 0.0093 0.0002 0.0013 0.0056 0.0056 0.008 0.0011 0.0004 0.0005 0.0018 0.0008 0.0005
Lipid Particles 0.0203 0.0004 0.0099 0.001 0.0402 0.0039 0.0178 0.0001 0.0008 0.0078 0.0033 0.0022 0.0004 0.0001 0.0005 0.0012 0.0001 0.0001
Mitochondria 0.0048 0.0003 0.0038 0.0066 0.0307 0.0047 0.0025 0.0001 0.0092 0.001 0.0023 0.0038 0.0011 0.0004 0.0003 0.001 0.0003 0.0004
None 0.0742 0.0338 0.0382 0.0188 0.01 0.0118 0.1328 0.0646 0.0526 0.0834 0.0044 0.0291 0.0947 0.0356 0.107 0.0357 0.0093 0.0537
Nuclear Periphery 0.0107 0.0025 0.0074 0.0021 0.0025 0.0045 0.0046 0.0009 0.0085 0.0035 0.023 0.008 0.0073 0.0019 0.0025 0.0036 0.0018 0.0022
Nucleolus 0.0015 0.0009 0.0027 0.0003 0.0056 0.0002 0.0017 0 0.0001 0.0011 0.0051 0.0004 0.0003 0.0003 0.0002 0.0001 0 0.0001
Nucleus 0.0171 0.0057 0.0143 0.0041 0.0089 0.0094 0.0138 0.0033 0.0059 0.0051 0.0396 0.0081 0.0112 0.009 0.0067 0.0102 0.0051 0.0118
Peroxisomes 0.0178 0.0024 0.0094 0.0006 0.0196 0.0079 0.0107 0.0001 0.0036 0.0006 0.0022 0.0036 0.0006 0.0002 0.0002 0.0032 0.0003 0.0003
Punctate Nuclear 0.0174 0.002 0.0064 0.001 0.0101 0.0032 0.015 0.0005 0.0038 0.0026 0.0091 0.004 0.0022 0.0025 0.003 0.0021 0.0008 0.0019
Vacuole 0.0035 0.0017 0.0045 0.0038 0.0076 0.0034 0.0052 0.0014 0.0017 0.0057 0.0069 0.0041 0.0024 0.0031 0.0018 0.0024 0.0016 0.0015
Vacuole Periphery 0.0011 0.0002 0.0011 0.0009 0.0018 0.0007 0.0009 0.0001 0.0009 0.0015 0.0012 0.0008 0.0006 0.0002 0.0002 0.0004 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.1622 83.1428 61.6956 79.5708 43.8502 126.7579 76.4233 76.0813 108.3717 89.0051
Translational Efficiency 2.9062 1.4352 2.1135 2.3814 2.4482 1.5418 2.0941 2.7226 1.7163 1.3543

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein; forms complex with Ups2p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (31%), nucleus (9%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mdm35

Mdm35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available