Standard name
Human Ortholog
Description RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – 0.05 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.98 1.0 0.96 0.93 0.9 0.8 0.76 0.74 0.75 0.71 0.97 0.97 0.98 0.85 – 0.89 0.97 0.96 0.96 0.83 0.81 0.82
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.07 0.18 0.17 0.17 0.18 0.22 0 0 0 0.05 – 0.11 0 0 0 0.06 0.13 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0.05 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 4 9 5 8 10 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3 2 0 1 1 0 0 0 0 – 0 0 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 2 3 6 3 8 20 10 13 6 11 1 2 1 1 – 1 0 0 0 0 0 0
Cytoplasm 86 45 206 257 320 274 348 374 179 263 176 162 209 255 382 34 – 17 94 67 195 106 258 302
Endoplasmic Reticulum 1 0 5 0 12 21 29 83 41 61 43 51 6 7 8 2 – 2 1 0 1 8 41 45
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 2 7 2 16 5 6 1 0 0 1 – 1 0 0 0 1 1 1
Mitochondria 0 0 0 0 0 0 0 0 3 5 6 3 0 0 0 2 – 0 0 0 0 1 2 1
Nucleus 1 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 – 0 0 0 1 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 0 2 4 0 8 5 2 0 1 0 2 – 1 0 1 2 6 5 6
Unique Cell Count 88 45 210 258 334 294 388 470 235 354 234 228 215 262 388 40 19 98 71 203 129 318 367
Labelled Cell Count 88 45 213 260 340 298 393 496 246 371 251 248 217 265 391 43 22 98 71 203 129 318 367


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 119.9 112.4 77.9 79.7 73.8 62.8 57.2 53.2 49.8 47.1 44.3 45.4 104.3 102.4 101.6 123.3 – 110.8 88.1 93.4 97.4
Std Deviation (1e-4) 25.8 23.1 15.1 17.8 13.5 14.1 12.2 11.5 13.3 11.3 11.2 9.8 17.5 21.8 17.3 72.3 – 50.5 14.8 20.5 23.7
Intensity Change (Log2) – – – 0.03 -0.08 -0.31 -0.45 -0.55 -0.65 -0.73 -0.81 -0.78 0.42 0.4 0.38 0.66 – 0.51 0.18 0.26 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 2.2 2.1 1.9 0 2.4 0 0 0 0 – 0
Cytoplasm 1.6 -1.5 -2.5 -3.8 -6.3 -6.8 -7.3 -6.9 -7.7 -0.6 -0.5 0.3 -3.9 – -2.3
Endoplasmic Reticulum 0 0.8 2.4 2.6 5.5 5.2 5.3 5.4 6.3 0.3 0.2 0 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 3.1 0 0 0 0 0 0 – 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 62.2461 86.3875 77.3327 78.0058 81.4189 55.8287 78.007 116.693 100.9519 99.2556 82.776 104.4108 90.1049 117.5853 104.1526 92.3019 102.9716 100.067
Actin 0.0108 0.001 0.0053 0.001 0.0004 0.0274 0.0066 0.0017 0.0062 0.0006 0.0012 0.0007 0.0136 0.0016 0.0074 0.0279 0.0554 0.0015
Bud 0.0034 0.0015 0.0022 0.0044 0.001 0.0034 0.0034 0.0005 0.002 0.0028 0.0043 0.0009 0.0013 0.0006 0.0027 0.0014 0.001 0.0008
Bud Neck 0.0008 0.0005 0.0017 0.001 0.0015 0.0029 0.0007 0.0004 0.001 0.0005 0.0009 0.0009 0.0008 0.0004 0.0008 0.001 0.0009 0.0008
Bud Periphery 0.0046 0.0009 0.002 0.0017 0.0006 0.0061 0.0041 0.0005 0.0011 0.0016 0.002 0.0002 0.0011 0.0003 0.0016 0.0012 0.0003 0.0006
Bud Site 0.0014 0.0021 0.0024 0.0011 0.0001 0.0056 0.0014 0.0004 0.003 0.0008 0.0012 0.0001 0.001 0.0005 0.0011 0.0009 0.0008 0.0003
Cell Periphery 0.0093 0.0108 0.0041 0.0067 0.0018 0.0097 0.0094 0.0061 0.0056 0.0116 0.0042 0.0002 0.0084 0.0021 0.0084 0.0049 0.0047 0.0106
Cytoplasm 0.5113 0.881 0.6893 0.5263 0.7536 0.5496 0.6126 0.9471 0.8185 0.7022 0.8047 0.8988 0.6956 0.9419 0.7787 0.6375 0.8175 0.7044
Cytoplasmic Foci 0.0193 0.0089 0.0083 0.0028 0.0034 0.0117 0.0073 0.0009 0.0069 0.0049 0.0104 0.0265 0.0065 0.0018 0.0164 0.0049 0.004 0.0243
Eisosomes 0.0005 0.0001 0.0008 0 0 0.0007 0.0002 0.0002 0.0018 0 0.0001 0 0.0002 0 0.0002 0.0003 0.0002 0.0013
Endoplasmic Reticulum 0.0551 0.0126 0.0106 0.0018 0.0353 0.0546 0.0386 0.0115 0.0136 0.0095 0.0043 0.0026 0.0212 0.0077 0.0129 0.0041 0.001 0.0245
Endosome 0.0297 0.005 0.0202 0.0148 0.019 0.0668 0.0283 0.0012 0.0055 0.039 0.0193 0.0168 0.0162 0.0036 0.0124 0.0147 0.0056 0.0148
Golgi 0.0047 0.0009 0.0046 0.0005 0.0006 0.0183 0.0052 0.0004 0.0011 0.0025 0.0014 0.0013 0.0045 0.0044 0.0048 0.0037 0.0051 0.0019
Lipid Particles 0.0178 0.0009 0.0058 0.0005 0.0003 0.0144 0.0013 0.0008 0.0038 0.0006 0.0036 0.001 0.0148 0.0052 0.0099 0.0059 0.0058 0.0041
Mitochondria 0.0344 0.0029 0.0057 0.005 0.0021 0.0279 0.0227 0.0081 0.026 0.0068 0.0023 0.0025 0.013 0.0027 0.01 0.0049 0.0018 0.0207
None 0.0105 0.0018 0.0175 0.001 0.0004 0.0029 0.0028 0.0001 0.0023 0.0006 0.0048 0.0037 0.005 0.0002 0.0027 0.007 0.0002 0.0339
Nuclear Periphery 0.0758 0.0058 0.0096 0.0128 0.0211 0.0146 0.058 0.0016 0.0071 0.0065 0.0068 0.0021 0.0241 0.0024 0.0077 0.0149 0.0025 0.0708
Nucleolus 0.0063 0.0018 0.0008 0.0022 0.0002 0.01 0.0013 0.0001 0.0064 0.0004 0.0099 0.012 0.0061 0.0001 0.003 0.0016 0.0002 0.0122
Nucleus 0.0154 0.0024 0.0036 0.0196 0.0066 0.0108 0.0187 0.001 0.0049 0.0041 0.0147 0.0049 0.0127 0.0015 0.0044 0.009 0.0028 0.0103
Peroxisomes 0.0146 0.0003 0.0062 0.0002 0.0001 0.0047 0.0002 0 0.0006 0.0001 0.0002 0.0002 0.0055 0.0011 0.0041 0.0007 0.0056 0.001
Punctate Nuclear 0.0031 0.0002 0.001 0.0003 0.0001 0.009 0.0011 0 0.0012 0.0001 0.0007 0.0022 0.0008 0.0002 0.0069 0.0006 0.0002 0.0005
Vacuole 0.111 0.0469 0.1218 0.2829 0.1111 0.0914 0.1327 0.0162 0.0663 0.1692 0.0797 0.0152 0.1105 0.0191 0.087 0.1891 0.0741 0.0343
Vacuole Periphery 0.0602 0.0117 0.0766 0.1136 0.0408 0.0577 0.0434 0.0014 0.015 0.0355 0.0233 0.0071 0.037 0.0026 0.0169 0.0637 0.0102 0.0264

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 117.4576 100.9957 83.5269 142.7274 128.4131 120.8745 106.2778 130.5568 109.1978 136.932
Translational Efficiency 2.7735 2.6581 2.9831 1.3357 2.1234 2.9136 2.0477 1.8569 2.1335 2.0378

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Def1

Def1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Def1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available