Standard name | |
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Human Ortholog | |
Description | Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p; human homolog CBR4 complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 10 | 5 | 3 | 4 | 3 | 1 | 1 | 0 | 1 | 0 | 4 | 1 | 4 | 0 | – | 0 | 3 | 0 | 4 | 12 | 3 | 5 | 13 |
Bud | 2 | 4 | 10 | 4 | 8 | 10 | 19 | 13 | 22 | 29 | 20 | 25 | 3 | 6 | 5 | – | 2 | 4 | 0 | 0 | 3 | 3 | 11 | 9 |
Bud Neck | 1 | 3 | 12 | 4 | 7 | 7 | 7 | 11 | 2 | 3 | 3 | 3 | 1 | 6 | 4 | – | 0 | 0 | 1 | 3 | 3 | 1 | 4 | 5 |
Bud Site | 0 | 3 | 1 | 3 | 0 | 4 | 7 | 0 | 4 | 3 | 9 | 8 | 0 | 1 | 0 | – | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 3 | 3 | 1 | 3 | 3 | 3 | 2 | 6 | 3 | 3 | 0 | 3 | 2 | – | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 |
Cytoplasm | 32 | 118 | 158 | 112 | 189 | 126 | 264 | 199 | 142 | 155 | 129 | 79 | 88 | 217 | 296 | – | 41 | 56 | 19 | 66 | 63 | 87 | 121 | 113 |
Endoplasmic Reticulum | 2 | 2 | 1 | 4 | 3 | 7 | 1 | 2 | 1 | 3 | 2 | 3 | 1 | 1 | 2 | – | 3 | 13 | 1 | 0 | 2 | 1 | 1 | 7 |
Endosome | 1 | 1 | 18 | 9 | 40 | 33 | 60 | 52 | 8 | 21 | 4 | 16 | 9 | 13 | 20 | – | 23 | 28 | 11 | 12 | 27 | 11 | 12 | 23 |
Golgi | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 3 | 4 | 2 | 4 |
Mitochondria | 17 | 37 | 5 | 30 | 15 | 109 | 224 | 142 | 387 | 468 | 407 | 370 | 10 | 2 | 1 | – | 11 | 5 | 45 | 52 | 128 | 59 | 58 | 86 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 6 | 10 | 17 | 9 | 13 | 15 | 7 | 0 | 1 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 2 | 3 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 1 | 2 | 1 | 10 | 9 | 10 | 5 | 3 | 3 | 2 | 10 | 2 | 9 | 11 | – | 11 | 4 | 1 | 1 | 8 | 1 | 1 | 1 |
SpindlePole | 10 | 14 | 72 | 34 | 78 | 44 | 69 | 94 | 35 | 87 | 46 | 56 | 11 | 37 | 43 | – | 29 | 27 | 15 | 30 | 62 | 21 | 40 | 39 |
Vac/Vac Membrane | 1 | 1 | 4 | 1 | 35 | 33 | 51 | 45 | 26 | 36 | 16 | 17 | 3 | 1 | 7 | – | 10 | 18 | 0 | 6 | 3 | 6 | 9 | 13 |
Unique Cell Count | 62 | 166 | 260 | 184 | 338 | 326 | 595 | 500 | 528 | 694 | 550 | 492 | 118 | 277 | 369 | 97 | 125 | 102 | 185 | 333 | 210 | 283 | 334 | |
Labelled Cell Count | 69 | 195 | 291 | 211 | 390 | 396 | 728 | 588 | 641 | 834 | 659 | 604 | 129 | 301 | 393 | 131 | 164 | 102 | 185 | 333 | 210 | 283 | 334 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 3.4 | 5.4 | 5.1 | 5.7 | 4.6 | 4.4 | 4.9 | 3.8 | 4.0 | 3.7 | 3.9 | 4.7 | 5.0 | 5.0 | 4.4 | 5.7 | 5.6 | 6.5 | 6.7 | 6.3 |
Std Deviation (1e-4) | 0.7 | 0.5 | 1.5 | 1.8 | 1.5 | 1.5 | 1.4 | 1.7 | 1.3 | 0.8 | 1.0 | 0.8 | 0.9 | 1.0 | 1.3 | 1.1 | 2.1 | 1.5 | 3.2 | 3.1 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.06 | -0.24 | -0.31 | -0.13 | -0.52 | -0.45 | -0.54 | -0.48 | -0.22 | -0.13 | -0.12 | -0.29 | 0.07 | 0.06 | 0.26 | 0.31 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.391 | 4.5011 | 4.5544 | 4.1323 | 4.3162 | 4.3062 | 4.0758 | 3.6133 | 3.2994 | 3.4669 | 2.5134 | 3.0548 | -0.2743 | 0.3634 | 0.0374 | 0.0256 | -0.2122 | 0.3489 |
Actin | 0.062 | 0.0461 | 0.0287 | 0.0152 | 0.0163 | 0.0178 | 0.0933 | 0.037 | 0.0424 | 0.001 | 0.0073 | 0.0165 | 0.0606 | 0.0418 | 0.0402 | 0.057 | 0.0026 | 0.0146 |
Bud | 0.0022 | 0.0188 | 0.0082 | 0.0139 | 0.0099 | 0.0024 | 0.0027 | 0.0077 | 0.0038 | 0.0021 | 0.0025 | 0.004 | 0.002 | 0.0043 | 0.0033 | 0.0089 | 0.0055 | 0.001 |
Bud Neck | 0.0027 | 0.0018 | 0.0034 | 0.0039 | 0.0035 | 0.0155 | 0.0149 | 0.004 | 0.0143 | 0.0047 | 0.002 | 0.0199 | 0.0075 | 0.0032 | 0.0079 | 0.0075 | 0.0012 | 0.0275 |
Bud Periphery | 0.0044 | 0.0153 | 0.0139 | 0.0403 | 0.0249 | 0.0055 | 0.0051 | 0.0092 | 0.0053 | 0.0093 | 0.0071 | 0.0098 | 0.0037 | 0.0046 | 0.0057 | 0.0111 | 0.0058 | 0.0018 |
Bud Site | 0.0136 | 0.0562 | 0.018 | 0.0125 | 0.0387 | 0.0016 | 0.027 | 0.11 | 0.0388 | 0.0031 | 0.0019 | 0.0055 | 0.017 | 0.0487 | 0.0154 | 0.0082 | 0.0029 | 0.0017 |
Cell Periphery | 0.0034 | 0.003 | 0.0051 | 0.002 | 0.0026 | 0.0017 | 0.0024 | 0.0041 | 0.0018 | 0.0018 | 0.0018 | 0.0043 | 0.0025 | 0.0017 | 0.0071 | 0.0011 | 0.0004 | 0.0007 |
Cytoplasm | 0.0814 | 0.1518 | 0.1568 | 0.2035 | 0.1633 | 0.1478 | 0.0869 | 0.159 | 0.2562 | 0.2622 | 0.1789 | 0.2841 | 0.1572 | 0.1869 | 0.2597 | 0.2568 | 0.1795 | 0.1478 |
Cytoplasmic Foci | 0.1152 | 0.1083 | 0.0984 | 0.0706 | 0.1038 | 0.1296 | 0.1081 | 0.1211 | 0.0881 | 0.043 | 0.1749 | 0.1158 | 0.1463 | 0.1486 | 0.1494 | 0.1003 | 0.1681 | 0.2065 |
Eisosomes | 0.0021 | 0.0014 | 0.0016 | 0.0004 | 0.0003 | 0.0007 | 0.0013 | 0.0007 | 0.001 | 0.0003 | 0.0005 | 0.0006 | 0.0006 | 0.0006 | 0.0018 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0118 | 0.0047 | 0.005 | 0.0044 | 0.0078 | 0.0045 | 0.0158 | 0.0061 | 0.0108 | 0.0033 | 0.0059 | 0.0137 | 0.009 | 0.0039 | 0.0083 | 0.0043 | 0.0044 | 0.003 |
Endosome | 0.1676 | 0.1281 | 0.1139 | 0.072 | 0.1387 | 0.1284 | 0.1241 | 0.1184 | 0.1023 | 0.0308 | 0.0927 | 0.0961 | 0.1786 | 0.13 | 0.1222 | 0.1234 | 0.1455 | 0.1983 |
Golgi | 0.0982 | 0.0778 | 0.0788 | 0.0302 | 0.0894 | 0.0675 | 0.058 | 0.0835 | 0.0361 | 0.0128 | 0.0476 | 0.0633 | 0.0677 | 0.0978 | 0.0612 | 0.0543 | 0.0412 | 0.0811 |
Lipid Particles | 0.0373 | 0.0073 | 0.0091 | 0.0104 | 0.0031 | 0.0143 | 0.0899 | 0.0108 | 0.0079 | 0.0123 | 0.0124 | 0.0216 | 0.0252 | 0.0192 | 0.0166 | 0.0055 | 0.066 | 0.025 |
Mitochondria | 0.1965 | 0.2142 | 0.2741 | 0.3883 | 0.2464 | 0.3158 | 0.1059 | 0.1596 | 0.1847 | 0.2938 | 0.2021 | 0.1853 | 0.1999 | 0.1986 | 0.1695 | 0.2747 | 0.2201 | 0.1951 |
None | 0.1271 | 0.1324 | 0.12 | 0.0657 | 0.0329 | 0.0803 | 0.1265 | 0.1063 | 0.1567 | 0.2622 | 0.1526 | 0.1001 | 0.0759 | 0.0689 | 0.1003 | 0.0254 | 0.1116 | 0.0269 |
Nuclear Periphery | 0.0062 | 0.001 | 0.0058 | 0.0093 | 0.0302 | 0.0034 | 0.0518 | 0.0012 | 0.0022 | 0.0006 | 0.0013 | 0.0026 | 0.0035 | 0.0013 | 0.0011 | 0.0009 | 0.0015 | 0.0075 |
Nucleolus | 0.0017 | 0.0007 | 0.0012 | 0.0015 | 0.0007 | 0.0007 | 0.0022 | 0.0009 | 0.0014 | 0.0007 | 0.0006 | 0.0003 | 0.0011 | 0.0007 | 0.0004 | 0.0009 | 0.0013 | 0.0006 |
Nucleus | 0.0029 | 0.0014 | 0.003 | 0.0054 | 0.0105 | 0.0036 | 0.007 | 0.0014 | 0.0031 | 0.0013 | 0.0011 | 0.001 | 0.0012 | 0.0013 | 0.0008 | 0.0019 | 0.0017 | 0.0023 |
Peroxisomes | 0.0392 | 0.0192 | 0.0363 | 0.0276 | 0.0462 | 0.0459 | 0.0455 | 0.0329 | 0.0292 | 0.0417 | 0.0894 | 0.0443 | 0.021 | 0.0251 | 0.0189 | 0.0112 | 0.0185 | 0.0428 |
Punctate Nuclear | 0.0072 | 0.0008 | 0.0023 | 0.003 | 0.0038 | 0.0015 | 0.0163 | 0.0014 | 0.0028 | 0.0005 | 0.0008 | 0.0011 | 0.0008 | 0.0014 | 0.0007 | 0.0008 | 0.0024 | 0.0023 |
Vacuole | 0.0124 | 0.0072 | 0.0099 | 0.015 | 0.0178 | 0.0074 | 0.011 | 0.0214 | 0.0082 | 0.0097 | 0.011 | 0.0072 | 0.0142 | 0.0088 | 0.0068 | 0.0384 | 0.0134 | 0.0078 |
Vacuole Periphery | 0.005 | 0.0026 | 0.0064 | 0.0049 | 0.0094 | 0.0039 | 0.0046 | 0.0033 | 0.0029 | 0.0029 | 0.0058 | 0.003 | 0.0045 | 0.0026 | 0.0025 | 0.0073 | 0.0064 | 0.0055 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.228 | 3.5354 | 5.2468 | 6.5732 | 6.8369 | 2.6351 | 8.9366 | 10.0527 | 4.6177 | 6.7283 |
Translational Efficiency | 0.3771 | 1.1775 | 0.5459 | 0.4807 | 0.4217 | 1.4596 | 0.7085 | 0.5734 | 0.7062 | 0.6569 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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