Standard name
Human Ortholog
Description Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0.08 0.05 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.99 0.98 0.95 – 0.81 0.87 0.84 0.82 0.85 0.99 1.0 0.99 1.0 1.0 0.99 0.8 0.96 0.95 0.95 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.15 0.08 0.12 0.13 0.1 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 – 6 5 7 10 7 0 0 0 0 0 0 1 0 0 0 0 0
Bud 4 1 10 0 0 0 – 2 1 3 4 5 2 1 2 0 0 0 159 0 0 6 8 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 20 2 6 0 8 13 – 27 35 25 41 23 6 1 11 6 2 4 1 0 1 0 0 0
Cytoplasm 1148 346 514 289 304 557 – 544 397 425 408 423 435 353 466 215 214 252 910 330 469 393 394 420
Endoplasmic Reticulum 7 0 0 0 6 26 – 102 35 63 64 50 3 1 0 13 17 13 10 0 7 3 2 4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 2 0 0 0 0 – 0 3 2 4 1 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 1 2 0 1 – 2 3 2 5 5 0 0 1 0 0 0 0 0 0 0 0 0
Nucleus 1 0 0 2 0 0 – 2 0 0 0 0 0 0 0 0 0 0 4 3 0 0 2 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 1 0 0 0 0 0 – 6 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Vac/Vac Membrane 3 0 0 0 0 0 – 1 0 1 0 0 0 0 1 2 1 1 16 2 1 1 2 2
Unique Cell Count 1161 348 516 292 311 587 670 456 508 497 496 438 354 470 215 215 254 1134 345 493 413 417 439
Labelled Cell Count 1186 351 531 293 318 597 693 479 530 536 516 446 357 481 236 234 271 1134 345 493 413 417 439


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 132.3 213.8 116.0 125.2 104.5 86.3 – 68.5 65.3 63.6 61.0 57.8 170.1 163.1 157.5 242.5 219.9 222.1 183.0 183.3 186.0
Std Deviation (1e-4) 52.0 42.6 29.1 37.5 27.2 21.1 – 18.6 18.3 19.4 18.7 16.5 49.7 44.6 40.6 78.3 77.0 77.2 44.8 49.0 52.0
Intensity Change (Log2) – – – 0.11 -0.15 -0.43 – -0.76 -0.83 -0.87 -0.93 -1.0 0.55 0.49 0.44 1.06 0.92 0.94 0.66 0.66 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – -2.8 -2.5 -1.9 -1.5 -1.2 -2.0 0 -2.2 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 1.3 – 3.0 5.0 3.5 5.4 3.3 0.3 0 1.4 0 0 0
Cytoplasm -1.1 -2.5 -4.7 – -10.1 -8.1 -9.3 -9.7 -8.7 -0.6 0.3 -0.9 0.9 -0.1 -0.7
Endoplasmic Reticulum 0 0 4.8 – 9.3 6.4 8.3 8.4 7.4 0 0 0 0 6.5 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 147.5211 222.0986 200.1359 180.5355 117.2878 193.9574 152.6247 205.3772 189.0301 194.3593 172.5687 171.7368 156.0218 213.1758 186.7015 171.2548 192.2878 207.6331
Actin 0.0096 0.0003 0.0051 0.0045 0.0706 0.0024 0.0062 0.0004 0.0009 0.0002 0.0004 0.0005 0.0035 0.0002 0.0217 0.0066 0.0001 0.0001
Bud 0.0015 0.0004 0.0007 0.0023 0.0022 0.0025 0.0012 0.0008 0.0005 0.0007 0.0004 0.0006 0.001 0.0008 0.0037 0.0009 0.0004 0
Bud Neck 0.0026 0.0006 0.0008 0.001 0.0034 0.0034 0.0007 0.0004 0.001 0.0009 0.0006 0.0019 0.0005 0.0002 0.0016 0.001 0.0006 0.0008
Bud Periphery 0.0012 0.0001 0.0004 0.0016 0.0037 0.0015 0.001 0.0008 0.0004 0.0015 0.0002 0.0002 0.0005 0.0004 0.0028 0.0004 0.0004 0
Bud Site 0.0021 0.0004 0.0007 0.0022 0.0115 0.0006 0.0014 0.0009 0.0026 0.0005 0.0002 0.0001 0.0005 0.0003 0.0048 0.0016 0.0001 0
Cell Periphery 0.0295 0.0247 0.0134 0.0155 0.001 0.0107 0.0201 0.0139 0.0229 0.036 0.0026 0.0036 0.0144 0.0118 0.0149 0.0063 0.0037 0.0001
Cytoplasm 0.6708 0.926 0.8337 0.7784 0.4933 0.6994 0.8076 0.9532 0.89 0.8617 0.9081 0.9123 0.8331 0.9592 0.8305 0.7402 0.9234 0.9941
Cytoplasmic Foci 0.0214 0.0005 0.0128 0.0063 0.0236 0.0034 0.0092 0.0007 0.0053 0.0005 0.0167 0.0232 0.027 0.0024 0.0073 0.0177 0.0009 0.0008
Eisosomes 0.001 0 0.0005 0 0.001 0.0004 0.0009 0 0.0003 0 0.0004 0 0.0009 0 0.0004 0.0018 0.0001 0
Endoplasmic Reticulum 0.0089 0.0023 0.002 0.0015 0.0026 0.0012 0.0164 0.0021 0.0038 0.0032 0.0023 0.0117 0.0145 0.0008 0.0027 0.0011 0.0009 0.0007
Endosome 0.0051 0.0002 0.0022 0.0099 0.0702 0.0022 0.0057 0.0003 0.0059 0.0008 0.0013 0.0051 0.0052 0.0003 0.0077 0.0262 0.0006 0.0005
Golgi 0.0031 0 0.0028 0.0036 0.0814 0.0005 0.0016 0.0002 0.0009 0.0001 0.0002 0.0006 0.0012 0.0001 0.0022 0.0182 0 0
Lipid Particles 0.0117 0.0006 0.0101 0.0166 0.102 0.0024 0.004 0.0007 0.0024 0.0022 0.0067 0.0012 0.005 0.001 0.0029 0.0261 0.0006 0
Mitochondria 0.0151 0.0002 0.0149 0.0065 0.083 0.0497 0.0074 0.0026 0.0027 0.0001 0.0155 0.0018 0.0084 0.0007 0.0028 0.0166 0.005 0
None 0.0117 0.0003 0.0035 0.0004 0.0012 0.0022 0.0128 0.0002 0.0053 0.0001 0.0034 0.0087 0.0121 0.001 0.0157 0.0121 0.0039 0.0001
Nuclear Periphery 0.0418 0.0048 0.0074 0.0102 0.0064 0.0162 0.0194 0.0026 0.0057 0.0048 0.0031 0.0034 0.0171 0.0024 0.0061 0.0071 0.0064 0.0008
Nucleolus 0.0329 0.0004 0.016 0.001 0.0021 0.0525 0.0121 0.0002 0.0078 0.0003 0.0036 0.0006 0.0105 0.0004 0.0044 0.022 0.0106 0
Nucleus 0.0319 0.0044 0.0092 0.0139 0.0039 0.0529 0.0129 0.0017 0.0069 0.0055 0.0035 0.0035 0.0085 0.0021 0.0109 0.014 0.0117 0.001
Peroxisomes 0.0019 0 0.0083 0.0133 0.0154 0.001 0.0005 0.0001 0.0004 0 0.0018 0.0003 0.0009 0.0002 0.0006 0.0058 0.0001 0
Punctate Nuclear 0.0088 0 0.0011 0.0009 0.004 0.0005 0.0008 0 0.0009 0 0.0005 0.0004 0.0013 0.0002 0.0007 0.0031 0.0002 0
Vacuole 0.0695 0.0316 0.0462 0.0933 0.0107 0.0337 0.0517 0.0176 0.0309 0.07 0.0258 0.0177 0.0294 0.015 0.0505 0.0554 0.0201 0.0008
Vacuole Periphery 0.0178 0.0019 0.0081 0.017 0.0067 0.0606 0.0064 0.0005 0.0024 0.0107 0.0029 0.0026 0.0046 0.0005 0.0053 0.0157 0.0103 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2552.7174 2849.5312 3241.4206 2331.0572 2502.1519 2588.5016 2261.2935 2072.1343 2297.1283 1809.8075
Translational Efficiency 1.1297 1.084 0.9601 1.3614 1.1978 1.1059 1.3803 1.5932 1.2934 1.5086

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tma19

Tma19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tma19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available