Standard name
Human Ortholog
Description Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 0.93 0.53 0.73 0.54 0.56 0.45 0.4 0.45 0.38 0.39 0.43 – – – 0.83 0.76 0.91 0.58 0.73 0.41 0.61 0.61 0.62
Cytoplasm – 0.14 0.55 0.31 0.56 0.55 0.67 0.68 0.64 0.69 0.73 0.68 – – – 0.21 0.27 0.09 0.4 0.22 0.56 0.35 0.36 0.36
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.07 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud – 1 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 3 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 39 60 72 88 73 89 70 84 85 64 64 – – – 70 57 21 72 161 85 61 81 68
Cytoplasm – 6 62 31 92 72 132 118 120 154 119 101 – – – 18 20 2 49 48 117 34 48 40
Endoplasmic Reticulum – 0 0 0 0 0 0 1 0 0 0 0 – – – 0 5 0 1 2 2 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 1 1 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 1 0 0 0 0 0 0 – – – 0 3 0 0 0 0 0 0 0
Unique Cell Count 42 113 99 164 131 197 173 188 222 163 148 84 75 23 124 220 210 101 134 111
Labelled Cell Count 46 122 103 180 146 221 189 204 239 183 165 88 85 23 124 220 210 101 134 111


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 740.6 676.8 679.3 629.7 583.7 560.8 551.8 516.7 515.6 477.1 488.8 – – – 719.9 740.2 719.4 696.2 681.8 –
Std Deviation (1e-4) – 130.2 131.7 113.0 135.1 100.6 109.0 113.6 114.7 106.7 92.6 97.8 – – – 151.8 179.6 110.3 190.2 202.8 –
Intensity Change (Log2) – – – 0.01 -0.1 -0.21 -0.27 -0.29 -0.39 -0.39 -0.5 -0.47 – – – 0.09 0.13 0.09 0.04 0.01 –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 2.9 0.1 0.4 -1.3 -2.1 -1.4 -2.6 -2.3 -1.6 – – – 4.4 3.2 3.4
Cytoplasm -3.4 0.2 0 2.1 2.3 1.5 2.6 3.1 2.2 – – – -4.7 -3.8 -4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0067 0 0.0031 0.0001 0.0149 0.0001 0.0005 0.0001 0.0003 0.0053 0.0001 0.0002 – – – – – –
Bud 0.0033 0.0007 0.0027 0.0008 0.012 0.0001 0.0048 0.0002 0.001 0.0149 0.0022 0.0003 – – – – – –
Bud Neck 0.0011 0 0.0006 0.0001 0.0007 0.0002 0.0003 0.0001 0.0003 0.0004 0.0002 0.0003 – – – – – –
Bud Periphery 0.0021 0.0001 0.0013 0.0002 0.0251 0 0.0018 0 0.0007 0.0072 0.0005 0.0001 – – – – – –
Bud Site 0.0018 0 0.0014 0.0002 0.0041 0 0.0013 0.0001 0.0003 0.0011 0.0002 0 – – – – – –
Cell Periphery 0.0245 0.0001 0.008 0.0019 0.0615 0 0.0016 0.0001 0.0013 0.0026 0.0019 0 – – – – – –
Cytoplasm 0.6018 0.975 0.7998 0.8242 0.4974 0.9822 0.8928 0.9787 0.8783 0.7962 0.6958 0.9578 – – – – – –
Cytoplasmic Foci 0.0028 0.0001 0.0044 0.0024 0.0008 0.0001 0.0052 0.0025 0.0287 0.0411 0.013 0.0005 – – – – – –
Eisosomes 0.0089 0 0.0029 0.0003 0.0051 0 0.0004 0 0.0006 0.0003 0.0005 0 – – – – – –
Endoplasmic Reticulum 0.0018 0 0.0004 0 0.0008 0 0.0007 0 0.0012 0.0016 0.0001 0.0001 – – – – – –
Endosome 0.0011 0 0.0011 0.0001 0.0043 0 0.0007 0 0.0089 0.0074 0.0001 0 – – – – – –
Golgi 0.0005 0 0.0003 0 0.0031 0 0.0001 0 0.0006 0.0013 0 0 – – – – – –
Lipid Particles 0.0038 0 0.0025 0.0009 0.0062 0 0.0005 0 0.0014 0.0059 0.0015 0 – – – – – –
Mitochondria 0.0589 0.0024 0.0435 0.0262 0.1604 0.0002 0.0193 0.0007 0.0088 0.0192 0.0313 0.0023 – – – – – –
None 0.0138 0.0005 0.0072 0.0037 0.0027 0.0002 0.0181 0.0016 0.0151 0.0068 0.0915 0.0056 – – – – – –
Nuclear Periphery 0.0894 0.0004 0.0164 0.0021 0.0028 0.0012 0.0064 0.0008 0.0024 0.0355 0.0025 0.0031 – – – – – –
Nucleolus 0.0434 0.0035 0.015 0.0578 0.0229 0 0.0109 0.0013 0.0267 0.0057 0.0322 0.0002 – – – – – –
Nucleus 0.0831 0.0168 0.0366 0.0656 0.0135 0.0156 0.0287 0.0134 0.015 0.0252 0.1139 0.0288 – – – – – –
Peroxisomes 0.0015 0 0.0011 0.0005 0.0021 0 0.0006 0 0.0004 0.0024 0.0005 0.0001 – – – – – –
Punctate Nuclear 0.0022 0 0.0006 0.0002 0.0001 0.0001 0.0004 0.0001 0.001 0.001 0.0008 0.0002 – – – – – –
Vacuole 0.0193 0.0002 0.0246 0.0054 0.0691 0 0.0041 0.0002 0.005 0.0114 0.0067 0.0002 – – – – – –
Vacuole Periphery 0.0282 0.0002 0.0266 0.0073 0.0905 0 0.001 0.0001 0.0019 0.0076 0.0045 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8097.7626 8484.6617 9377.5614 8544.1414 9128.0999 7558.2206 8993.9226 9032.0479 9776.2853 8620.2347
Translational Efficiency 1.4411 1.4695 1.3823 1.3396 1.4642 1.5478 1.6036 1.6605 1.5305 1.7172

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo
Localization
Cell Percentages cytoplasm (76%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fba1

Fba1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fba1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available