Standard name
Human Ortholog
Description Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0.05 0.05 0 0 0 0.06 0.07 0.06 0.09 0.06 0 0.06 0 0.1 0.09 0.11 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0.07 0.07 0.07 0.06 0 0 0.05 0.07 0.06 0.07 0 0.16 0.23 0.34 0.26 0.26 0 0 0 0 0 0
Cytoplasm – 0.1 0.07 0.11 0.11 0.1 0.06 0.09 0.11 0.08 0.07 0.08 0.08 0 0.07 0.31 0.23 0.31 0 0 0 0 0 0
Endoplasmic Reticulum – 0.86 0.61 0.43 0.47 0.77 0.86 0.47 0.65 0.68 0.68 0.66 0.92 0.82 0.88 0 0 0 0.93 0.88 0.93 0.87 0.86 0.85
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0.13 0.17 0.15 0 0 0.13 0 0 0 0.05 0 0 0 0.24 0.42 0.26 0 0 0 0 0 0
Mitochondria – 0 0.05 0 0 0.05 0 0 0.05 0 0.07 0.06 0 0 0 0.07 0 0.11 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0.08 0 0 0.05 0.06 0 0.05 0 0 0.05 0.09 0.07 0 0 0 0 0.06 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.1 0.25 0.35 0.36 0.13 0.07 0.38 0.15 0.18 0.15 0.15 0 0 0 0.08 0.17 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 5 19 20 20 25 26 49 44 36 54 39 2 5 2 8 5 8 0 2 4 1 3 2
Bud – 2 2 3 2 4 3 13 10 3 10 6 0 0 0 1 0 1 0 3 1 1 0 1
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 6 30 28 34 36 31 28 31 41 37 44 1 13 21 27 14 19 0 0 3 0 0 3
Cytoplasm – 22 31 42 54 63 47 69 68 49 43 52 5 3 6 25 12 23 0 5 3 0 1 2
Endoplasmic Reticulum – 196 254 166 227 500 694 383 393 398 428 435 59 66 79 1 2 0 28 236 449 71 114 115
Endosome – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Golgi – 1 53 67 74 12 23 102 16 17 13 36 0 0 0 19 22 19 0 0 1 0 2 2
Mitochondria – 0 22 15 10 30 30 10 30 16 44 39 1 0 0 6 1 8 0 1 2 0 3 1
Nucleus – 0 0 1 1 0 1 1 0 0 1 0 0 0 0 0 0 0 0 3 0 0 0 0
Nuclear Periphery – 8 35 16 14 35 50 8 33 23 27 36 6 6 1 0 0 0 1 2 8 1 1 0
Nucleolus – 0 0 1 1 0 2 0 1 1 1 1 0 0 0 0 1 0 0 0 1 0 0 0
Peroxisomes – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 22 105 135 176 86 53 306 91 106 96 102 0 1 0 6 9 10 0 7 0 1 4 3
Unique Cell Count 229 414 387 486 648 807 810 601 584 633 660 64 80 90 80 53 74 31 268 482 82 134 136
Labelled Cell Count 262 552 495 613 791 960 970 718 690 754 791 74 94 109 93 66 88 31 268 482 82 134 136


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 17.7 18.3 18.7 20.7 18.7 20.0 22.3 21.0 21.6 20.8 22.0 16.7 19.1 20.6 27.7 26.7 26.0 14.3 19.0 21.2
Std Deviation (1e-4) – 4.8 4.4 5.4 6.8 3.7 4.4 5.0 4.6 5.0 4.9 5.8 3.5 3.6 3.5 12.4 10.8 11.1 4.9 5.6 5.5
Intensity Change (Log2) – – – 0.03 0.17 0.03 0.12 0.28 0.2 0.24 0.18 0.26 -0.14 0.06 0.17 0.59 0.54 0.51 -0.36 0.05 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 -0.3 -0.6 -1.2 1.1 1.8 1.1 2.4 0.9 0 0 0 0 0 0
Bud 0 0 0 0 1.7 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.1 -1.1 -2.6 -3.0 -1.4 -0.1 -0.9 -0.4 0 2.6 4.6 6.8 0 4.9
Cytoplasm 1.7 1.9 1.3 -1.1 0.6 2.0 0.5 -0.4 0.2 0 -1.2 -0.3 6.1 0 6.0
Endoplasmic Reticulum -5.2 -4.4 5.5 9.8 -4.7 1.3 2.2 2.1 1.5 4.8 3.6 4.8 -9.8 -7.9 -9.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.8 1.0 -7.3 -6.8 -0.1 -6.3 -6.0 -7.0 -4.3 -3.0 -3.4 -3.6 2.5 5.4 2.9
Mitochondria -1.0 -2.6 -0.5 -1.3 -4.2 -0.2 -2.1 1.1 0.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.5 -3.7 -2.0 -1.5 -6.7 -1.9 -3.0 -2.8 -1.9 0.2 -0.3 -2.5 -2.7 0 -2.6
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.9 3.5 -5.0 -9.3 4.4 -4.1 -2.7 -4.1 -4.0 -4.6 -4.8 -5.4 -3.5 -1.3 -2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.7535 17.1029 18.1463 18.111 18.0861 18.3448 23.4867 23.5577 22.869 21.2319 20.9477 22.2598 19.6357 19.8837 17.3506 16.8244 16.7625 17.4454
Actin 0.0004 0.0013 0.0001 0.0062 0.0002 0 0.009 0 0.0006 0 0 0.0015 0.0093 0.0043 0.0041 0.0427 0.0273 0.0071
Bud 0 0 0 0.0005 0 0 0.0001 0 0 0 0 0 0.0001 0.0002 0.0006 0.0125 0.0001 0
Bud Neck 0.0001 0.0002 0.0002 0.0064 0.0034 0.0002 0.0002 0.0001 0 0 0 0.0002 0.0013 0.0026 0.0006 0.0014 0.0003 0.0019
Bud Periphery 0.0001 0.0001 0.0001 0.0009 0.0001 0 0.0002 0 0.0001 0 0 0.0001 0.0002 0.0011 0.0005 0.0132 0.0001 0.0002
Bud Site 0.0001 0.0009 0.0002 0.0015 0 0 0.0004 0 0.0001 0 0 0.0001 0.0045 0.0022 0.0118 0.0089 0.0001 0
Cell Periphery 0.0017 0.0036 0.0053 0.002 0.0022 0.0023 0.0027 0.0022 0.0021 0.0018 0.0009 0.001 0.0008 0.0225 0.0011 0.0015 0.0002 0.0006
Cytoplasm 0.0093 0.0097 0.0062 0.0084 0.0203 0.0038 0.0022 0.0064 0.0006 0.0003 0.0003 0.0018 0.0257 0.0032 0.0136 0.0018 0.0037 0.0247
Cytoplasmic Foci 0.0008 0.0003 0.0038 0.0003 0.0001 0.0031 0.0023 0 0.0006 0 0 0.0009 0.0144 0.0056 0.0015 0.0004 0.0018 0.0001
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0002 0 0 0.0001 0.0002 0.0034 0 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.9069 0.9481 0.9594 0.9636 0.9612 0.9683 0.8717 0.9698 0.9629 0.9826 0.9882 0.9744 0.6327 0.8429 0.8999 0.8327 0.8486 0.8184
Endosome 0.0004 0.0009 0.0007 0.0003 0.0002 0.0004 0.0063 0.0001 0.0026 0.0007 0.0004 0.0015 0.0029 0.0082 0.0035 0.0018 0.0259 0.0016
Golgi 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.002 0 0.0011 0.0004 0.0001 0.0004 0.0009 0.0232 0.0011 0.0021 0.0042 0.0006
Lipid Particles 0.0012 0.0073 0.0019 0.0006 0.0002 0.0004 0.0087 0.0003 0.0058 0.0008 0.0002 0.0011 0.0011 0.0055 0.0012 0.0006 0.002 0.0002
Mitochondria 0.0001 0 0.0001 0.0001 0 0 0.0027 0 0.0089 0.0018 0 0.0003 0.0003 0.0025 0.0005 0.0022 0.0012 0.0003
None 0.0005 0 0 0 0 0.0004 0.0011 0 0.0002 0 0 0.0004 0.0113 0.0007 0.0108 0.0002 0.0025 0.0003
Nuclear Periphery 0.0692 0.0204 0.016 0.006 0.0071 0.0154 0.0739 0.0183 0.0101 0.0081 0.008 0.0127 0.2608 0.0593 0.0408 0.0668 0.0676 0.1229
Nucleolus 0.0001 0.0001 0.0003 0.0001 0 0 0.0003 0 0.0002 0 0 0.0001 0.0006 0.0005 0.0001 0.0001 0.0002 0
Nucleus 0.0029 0.0008 0.0008 0.001 0.0014 0.0024 0.0022 0.0005 0.0003 0.0001 0.0002 0.0007 0.0162 0.0038 0.0026 0.0012 0.0044 0.0135
Peroxisomes 0 0 0 0 0 0 0.0022 0 0.0005 0 0 0.0001 0.002 0.0004 0.0003 0.0003 0.0003 0
Punctate Nuclear 0.001 0.0001 0.0004 0.0002 0 0.0004 0.0007 0 0.0001 0 0 0.0009 0.006 0.0002 0.0005 0.0002 0.0062 0.0003
Vacuole 0.003 0.0054 0.0038 0.0014 0.0032 0.0023 0.0072 0.0017 0.0019 0.0019 0.0013 0.0014 0.0043 0.0056 0.0036 0.002 0.0016 0.0039
Vacuole Periphery 0.0019 0.0002 0.0005 0.0002 0.0002 0.0003 0.0036 0.0004 0.0012 0.0012 0.0003 0.0003 0.0043 0.0022 0.0012 0.007 0.0014 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 116.3816 77.83 81.326 98.4128 78.3266 83.4642 120.0483 105.6841 112.5957 109.2955
Translational Efficiency 1.0616 1.0318 0.9648 0.8132 1.049 1.7409 0.9553 0.8661 0.9501 0.8782

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Yet1

Yet1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yet1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available