Standard name
Human Ortholog
Description Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.12 0.11 0.15 0.14 0.13 0.12 0.11
Bud 0.05 0 0 0 0 0.05 0 0.05 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0 0.07 0.09 0.11
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 0.09 0.07 0.13 0.08 0.06 0 0 0 0 0.06 0 0.06 0.06 0.11 0.24 0.17 0.19 0.18 0.15 0.15 0.2 0.16 0.17
Cytoplasm 0.8 0.86 0.82 0.85 0.88 0.7 0.6 0.63 0.6 0.59 0.57 0.54 0.97 0.98 0.99 0.76 0.76 0.78 0.18 0.34 0.27 0.29 0.31 0.36
Endoplasmic Reticulum 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0.23 0.15 0.18 0.08 0.07 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.1 0.09 0.13 0.34 0.52 0.43 0.5 0.49 0.55 0.58 0 0 0 0 0 0 0.08 0 0.15 0.12 0.12 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 7 2 9 8 15 4 5 2 6 4 4 4 3 3 27 28 30 9 17 17 20 35 37
Bud 2 3 4 9 13 33 34 39 32 30 36 44 2 1 1 4 7 4 0 2 1 1 7 3
Bud Neck 0 1 3 0 2 4 2 2 0 1 0 0 5 4 3 0 0 1 6 11 4 11 26 36
Bud Site 0 0 0 0 1 6 0 1 2 5 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 8 7 43 37 41 16 23 20 29 38 38 18 29 57 52 36 47 15 18 19 30 45 57
Cytoplasm 32 81 81 272 396 446 478 506 327 430 349 481 273 464 512 168 161 195 15 40 35 44 89 123
Endoplasmic Reticulum 0 6 5 3 10 27 15 6 9 9 4 19 3 3 4 10 10 18 19 17 23 11 21 20
Endosome 0 0 0 1 1 0 0 2 0 1 3 1 0 0 0 0 4 0 3 1 2 0 3 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0
Mitochondria 2 1 10 29 58 217 420 347 272 359 336 518 0 2 0 6 7 5 7 3 19 17 35 36
Nucleus 0 0 0 1 0 3 5 11 4 2 3 4 1 1 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 27 1 1 2 0 3 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 1 1 1 2 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 2 2 0 3 0 1 2 1 1 0 0 0 0 2 1 0 0 1 0 0 0
SpindlePole 0 0 0 2 1 1 8 6 2 11 3 5 0 1 0 2 3 7 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 2 0 6 8 3 13 6 10 0 1 1 0 1 3 4 1 3 9 11 18
Unique Cell Count 40 94 99 320 450 639 802 809 547 731 612 888 280 472 517 220 212 251 87 117 131 153 288 346
Labelled Cell Count 44 107 112 371 533 794 1019 959 675 900 784 1132 306 509 582 270 259 311 87 117 131 153 288 346


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.8 4.0 4.1 4.1 3.8 3.3 3.8 3.5 3.4 3.4 3.4 5.8 5.6 5.9 6.3 6.4 6.7 6.4 6.7 6.7
Std Deviation (1e-4) 0.5 1.0 0.5 1.0 1.3 1.1 1.0 1.1 1.0 0.9 1.0 0.8 1.1 0.9 1.3 0.8 0.9 1.1 1.4 2.0 1.6
Intensity Change (Log2) – – – 0.03 0.01 -0.08 -0.3 -0.09 -0.21 -0.25 -0.24 -0.24 0.52 0.46 0.54 0.65 0.67 0.74 0.66 0.73 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2.9 3.1 2.9
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 -0.2 -0.3 1.2 3.5 2.4 2.7
Cytoplasm 0.8 1.6 -2.5 -4.3 -3.8 -4.2 -4.4 -4.7 -5.3 5.4 7.2 8.3 -1.1 -1.2 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 -2.5 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0045 0.005 0.0053 0.0078 0.0066 0.0039 0.0071 0.0027 0.0059 0.0151 0.0241 0.0036 – – – – – –
Bud 0.0042 0.0087 0.0052 0.0357 0.0314 0.0023 0.0003 0.0009 0.0005 0.003 0.0007 0.0002 – – – – – –
Bud Neck 0.0019 0.0024 0.0038 0.0025 0.0029 0.0044 0.0009 0.0008 0.0018 0.0008 0.0075 0.0071 – – – – – –
Bud Periphery 0.0028 0.0027 0.0043 0.0128 0.0076 0.0029 0.002 0.0027 0.0023 0.006 0.002 0.0009 – – – – – –
Bud Site 0.0057 0.0656 0.0284 0.0148 0.0101 0.0007 0.0021 0.0324 0.0084 0.0045 0.0033 0.0002 – – – – – –
Cell Periphery 0.0056 0.0049 0.0042 0.0042 0.0019 0.0029 0.0158 0.0133 0.0126 0.005 0.0077 0.006 – – – – – –
Cytoplasm 0.4476 0.4633 0.4506 0.462 0.5141 0.4763 0.5145 0.5259 0.5522 0.3223 0.5732 0.5903 – – – – – –
Cytoplasmic Foci 0.0108 0.0171 0.019 0.0084 0.0132 0.01 0.0144 0.0073 0.0107 0.0101 0.0148 0.0051 – – – – – –
Eisosomes 0.0009 0.0009 0.0009 0.0011 0.0007 0.0004 0.0011 0.0023 0.0007 0.0009 0.0017 0.0005 – – – – – –
Endoplasmic Reticulum 0.0098 0.0085 0.0106 0.0113 0.0082 0.0068 0.0119 0.0115 0.0123 0.0107 0.0066 0.0093 – – – – – –
Endosome 0.0043 0.0073 0.0078 0.0031 0.0032 0.0026 0.0044 0.0029 0.0067 0.0108 0.0213 0.0014 – – – – – –
Golgi 0.0009 0.0034 0.0015 0.0015 0.0017 0.001 0.0013 0.0007 0.0028 0.0044 0.0043 0.0003 – – – – – –
Lipid Particles 0.0026 0.0016 0.0026 0.0008 0.0014 0.0008 0.0077 0.0049 0.0081 0.0037 0.0049 0.0005 – – – – – –
Mitochondria 0.0015 0.0012 0.0018 0.0035 0.0025 0.001 0.0007 0.001 0.0074 0.0038 0.0041 0.0002 – – – – – –
None 0.4838 0.3945 0.4392 0.409 0.3722 0.4756 0.3945 0.3847 0.3486 0.5894 0.2949 0.3724 – – – – – –
Nuclear Periphery 0.001 0.0007 0.0017 0.0019 0.0023 0.0008 0.0027 0.0004 0.0006 0.0013 0.0084 0.0002 – – – – – –
Nucleolus 0.0015 0.0009 0.0014 0.0008 0.001 0.0006 0.0005 0.0005 0.0003 0.0003 0.002 0.0001 – – – – – –
Nucleus 0.003 0.0047 0.0044 0.0134 0.0076 0.0025 0.0013 0.0008 0.0006 0.0008 0.0062 0.0003 – – – – – –
Peroxisomes 0.0003 0.0009 0.0017 0.0004 0.0007 0.0003 0.0051 0.0009 0.0099 0.0013 0.0031 0.0001 – – – – – –
Punctate Nuclear 0.0053 0.0034 0.003 0.0021 0.0091 0.002 0.0084 0.0005 0.0042 0.0006 0.0049 0.0001 – – – – – –
Vacuole 0.002 0.0023 0.0025 0.0026 0.0014 0.0021 0.0032 0.0028 0.0031 0.0044 0.0029 0.0009 – – – – – –
Vacuole Periphery 0.0002 0.0002 0.0003 0.0004 0.0003 0.0002 0.0002 0.0001 0.0002 0.0006 0.0014 0 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7135 4.7154 7.6342 9.3272 10.5695 9.1061 10.0773 12.1403 9.3912 11.6254
Translational Efficiency 0.8105 1.4318 0.7416 0.5357 0.477 0.4275 0.6041 0.4821 0.6285 0.4837

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication
Localization
Cell Percentages ER (27%), cytoplasm (8%), mixed (53%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Stb6

Stb6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stb6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available