Standard name
Human Ortholog
Description Kinesin-like myosin passenger-protein; interacts with Myo2p and enhances its interaction with Sec4p during transport of secretory vesicles; controls actin cable structure and dynamics
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.25 0.22 0.33 0.23 0.16 0.19 – 0.12 0.09 0.12 0.1 – 0.14 0.17 0.25 0.18 0.12 0.19 0.22 0.15 0.15 0.13 0.13
Bud 0.18 0.17 0.15 0.18 0.19 0.18 0.15 – 0.12 0.17 0.16 0.14 – 0.06 0.1 0.09 0.07 0.08 0.31 0.24 0.2 0.24 0.42 0.39
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0.09 0.13 0.12 0.11 0.17 0.15
Bud Site 0.14 0.08 0.07 0.06 0.06 0.11 0.09 – 0 0 0 0.05 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.05 0 0 0 – 0 0.08 0.06 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0.36 0.4 0.54 0.29 0.34 0.35 0.39 – 0.38 0.36 0.31 0.37 – 0.66 0.73 0.34 0.34 0.32 0.21 0.2 0.21 0.1 0.06 0.15
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 – 0.05 0 0.05 0 – 0.05 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.09 0 0.09 0.05 0.08 0 – 0 0.06 0 0.07 – 0 0 0.08 0.09 0.17 0.06 0.05 0.08 0 0.07 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.08 0.07 0 0 0 0 0 0 0
Mitochondria 0.19 0.05 0.05 0 0 0.14 0.13 – 0.29 0.28 0.42 0.27 – 0 0 0.11 0.06 0.08 0 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.13 0.12 0 0.1 0.1 0.14 0.07 – 0.05 0 0 0.05 – 0.07 0 0 0.09 0.08 0 0 0 0.06 0 0
SpindlePole 0.05 0 0.07 0.2 0.22 0.19 0.11 – 0.06 0.1 0.05 0.08 – 0.07 0 0.09 0.15 0.17 0 0 0 0.13 0.07 0.06
Vac/Vac Membrane 0 0 0.07 0 0.05 0 0.07 – 0.08 0.09 0.08 0.06 – 0 0 0.16 0.1 0.13 0 0 0 0.08 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 25 32 9 56 69 38 60 – 25 25 43 46 – 15 19 54 59 35 26 35 13 3 23 35
Bud 20 22 6 31 55 43 47 – 26 48 57 60 – 6 12 20 21 24 43 38 17 5 76 102
Bud Neck 0 1 0 4 4 1 4 – 1 4 1 4 – 0 0 0 6 0 11 20 10 2 30 41
Bud Site 16 11 3 10 19 26 29 – 9 12 11 24 – 4 1 6 13 13 – – – – – –
Cell Periphery 1 4 1 7 3 2 5 – 10 7 13 11 – 2 9 12 32 20 0 0 0 0 0 0
Cytoplasm 41 52 22 49 100 84 122 – 80 103 109 164 – 71 84 74 111 96 28 33 18 2 11 39
Endoplasmic Reticulum 1 6 1 7 3 9 12 – 10 9 16 14 – 5 0 8 9 13 3 3 2 0 0 3
Endosome 10 12 1 15 14 20 14 – 9 16 14 33 – 4 1 17 28 51 8 8 6 0 13 9
Golgi 0 5 0 0 3 2 1 – 0 0 4 3 – 1 1 17 23 11 2 1 0 0 0 0
Mitochondria 22 7 2 6 8 33 41 – 61 81 145 119 – 0 0 24 20 24 4 6 9 0 4 4
Nucleus 2 1 1 1 5 3 7 – 2 5 0 3 – 0 0 1 4 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 1 1 3 4 13 – 3 11 5 6 – 0 0 0 1 5 0 0 0 0 0 0
Peroxisomes 15 15 0 17 30 33 22 – 11 10 12 21 – 7 5 8 30 23 1 1 0 1 4 5
SpindlePole 6 4 3 34 64 45 35 – 12 29 17 36 – 8 3 19 49 52 1 3 3 2 12 16
Vac/Vac Membrane 2 5 3 4 15 9 23 – 17 25 27 26 – 2 4 34 31 39 2 6 2 1 3 3
Unique Cell Count 113 130 41 168 296 242 314 209 290 348 442 107 115 217 322 299 138 162 87 21 182 266
Labelled Cell Count 161 178 53 242 395 352 435 276 385 474 571 125 139 294 437 412 138 162 87 21 182 266


Ambiguous, Bud, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.2 7.1 7.5 7.9 7.1 7.1 – 6.5 6.8 6.3 6.6 – 8.7 8.7 10.3 10.3 10.0 – 7.4 7.6
Std Deviation (1e-4) 0.8 1.1 1.2 1.0 1.5 1.4 1.8 – 1.4 1.5 1.3 1.4 – 1.1 1.4 2.0 1.9 2.2 – 1.1 1.0
Intensity Change (Log2) – – – 0.09 0.17 0.01 0.02 – -0.12 -0.05 -0.16 -0.09 – 0.29 0.29 0.55 0.54 0.5 – 0.06 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.4 0.2 -1.0 -0.4 – -1.7 0 -1.7 0 – -1.2 -0.8 0.4 -0.6 -1.8
Bud 0.6 0.6 0.5 0.1 – -0.4 0.3 0.3 -0.2 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cytoplasm -3.0 -2.5 -2.3 -1.8 – -1.8 -2.2 -2.9 -2.1 – 1.4 2.3 -2.4 -2.4 -2.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – 0 0 0 0 2.4
Golgi 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 1.6 0 – 3.3 3.2 4.6 3.1 – 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 1.9 2.2 1.8 0 – 0 0 0 0 – 0 0 0 1.4 1.6
Vacuole 0 0 0 0 – 0 0 0 0 – 0 0 1.4 0 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5869 3.3819 2.9228 3.4572 2.3901 3.0058 3.4741 4.2539 4.1594 3.3851 3.7584 4.1336 1.299 1.9264 1.7782 1.7575 1.626 2.0494
Actin 0.2129 0.052 0.176 0.2946 0.3239 0.0361 0.1287 0.0553 0.1824 0.0651 0.2819 0.08 0.1119 0.0488 0.1775 0.1461 0.2623 0.1003
Bud 0.0267 0.0203 0.1923 0.0917 0.0454 0.0074 0.0647 0.0573 0.2424 0.15 0.1031 0.0127 0.0586 0.0642 0.2323 0.1419 0.0238 0.0098
Bud Neck 0.0387 0.0087 0.0121 0.0092 0.0447 0.5925 0.0302 0.0067 0.0015 0.0018 0.0162 0.495 0.0372 0.0091 0.0037 0.0025 0.0021 0.4935
Bud Periphery 0.0427 0.0115 0.2446 0.1448 0.092 0.0071 0.0554 0.0339 0.2972 0.3363 0.1723 0.0056 0.0804 0.0405 0.2714 0.2599 0.0388 0.011
Bud Site 0.0917 0.4289 0.0951 0.0319 0.0084 0.0122 0.1541 0.5103 0.1062 0.0135 0.0187 0.006 0.1875 0.4439 0.0642 0.0304 0.0107 0.0092
Cell Periphery 0.003 0.0016 0.0017 0.0015 0.0004 0.0007 0.0027 0.0023 0.0016 0.0014 0.0026 0.0006 0.0021 0.0014 0.0012 0.0015 0.0007 0.0007
Cytoplasm 0.1052 0.2767 0.1076 0.2381 0.1568 0.1324 0.1439 0.1965 0.0919 0.1383 0.2258 0.1494 0.1853 0.2181 0.12 0.1736 0.3169 0.1356
Cytoplasmic Foci 0.2196 0.0895 0.0473 0.0292 0.0636 0.1385 0.2201 0.0938 0.0226 0.0779 0.0474 0.1219 0.169 0.0697 0.0339 0.0362 0.0824 0.1454
Eisosomes 0.0013 0.0002 0.0002 0.0004 0.0006 0.0001 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0079 0.002 0.0062 0.0033 0.0011 0.0027 0.0156 0.0007 0.0019 0.003 0.0025 0.0022 0.0068 0.0078 0.0012 0.0057 0.0023 0.0019
Endosome 0.0483 0.0201 0.0421 0.0603 0.0566 0.0349 0.039 0.0053 0.0112 0.0603 0.025 0.0356 0.0567 0.0437 0.026 0.1015 0.0881 0.0248
Golgi 0.046 0.008 0.0241 0.021 0.093 0.017 0.0242 0.0054 0.0081 0.0532 0.0283 0.0366 0.0283 0.017 0.0116 0.0275 0.0307 0.02
Lipid Particles 0.018 0.0018 0.008 0.0033 0.0481 0.0011 0.0131 0.0024 0.0015 0.0307 0.0026 0.0038 0.0095 0.0011 0.0063 0.0024 0.0322 0.0101
Mitochondria 0.0198 0.0017 0.0105 0.0188 0.0205 0.0015 0.0138 0.001 0.0057 0.0187 0.028 0.0056 0.0046 0.0026 0.0068 0.0095 0.0103 0.0061
None 0.045 0.0311 0.0061 0.0043 0.0011 0.0016 0.0105 0.0015 0.0135 0.0026 0.0275 0.0014 0.0131 0.0084 0.0064 0.0064 0.0219 0.0033
Nuclear Periphery 0.0076 0.0013 0.0059 0.0022 0.0007 0.0003 0.0101 0.0002 0.0005 0.0009 0.0008 0.0018 0.0035 0.0009 0.0011 0.0052 0.0012 0.0005
Nucleolus 0.0015 0.0012 0.0004 0.0027 0.0001 0.0002 0.0013 0.0005 0.0002 0.0002 0.0008 0.0006 0.0035 0.0012 0.0018 0.0005 0.001 0.0019
Nucleus 0.0038 0.0035 0.0022 0.0026 0.0003 0.0005 0.0177 0.001 0.0005 0.0007 0.0012 0.0031 0.0025 0.0038 0.0021 0.0022 0.0012 0.0008
Peroxisomes 0.0312 0.0079 0.01 0.0067 0.036 0.0073 0.0383 0.0181 0.0075 0.0281 0.0048 0.034 0.0179 0.0078 0.02 0.006 0.0363 0.0204
Punctate Nuclear 0.0183 0.008 0.0021 0.0174 0.0016 0.0023 0.0059 0.0035 0.0006 0.0009 0.0023 0.0018 0.0077 0.0032 0.0064 0.0015 0.0275 0.0026
Vacuole 0.009 0.021 0.0041 0.0132 0.0029 0.0028 0.0085 0.0041 0.0022 0.0112 0.0061 0.0014 0.0117 0.0057 0.0044 0.0315 0.0065 0.0015
Vacuole Periphery 0.002 0.003 0.0015 0.0028 0.0021 0.0006 0.0017 0.0003 0.0007 0.005 0.0019 0.0006 0.0018 0.0011 0.0015 0.0077 0.0026 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0361 21.0187 13.74 16.1446 15.2424 11.7497 17.71 20.3202 16.4007 16.9946
Translational Efficiency 0.7502 0.6038 0.8748 0.7025 0.6106 0.9197 0.8175 0.6639 0.744 0.7464

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Kinesin-like myosin passenger-protein; interacts with Myo2p and enhances its interaction with Sec4p during transport of secretory vesicles; controls actin cable structure and dynamics
Localization
Cell Percentages cytoplasm (21%), actin (18%), mixed (25%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Smy1

Smy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available