Standard name
Human Ortholog
Description Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.16 0.1 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.11 0.1 0.07 0.07 0.11 0 0.06 0.06 0.05 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 0.28 0.28 0.37 0.05 0.06 0.12 0 0.13 0 0.07 0.07 0.23 0.35 0.31 0.28 0.05 0 0.13 0.06 0.07 0.08 0.07
Mitochondria 0.96 0.94 0.7 0.66 0.55 0.75 0.75 0.65 0.82 0.67 0.87 0.82 0.9 0.79 0.74 0.11 0.09 0.15 0.85 0.77 0.7 0.78 0.68 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0.07 0 0.1 0.14 0.13 0.14 0.12 0.09 0.11 0 0 0 0.12 0.25 0.33 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0.15 0.09 0.15 0.05 0 0 0.05 0.07 0.11
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.08 0.06 0.1 0.14 0.1 0.05 0.09 0.06 0.14 0 0.06 0 0.08 0.07 0.17 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 9 5 2 1 2 4 1 2 1 2 4 6 4 8 5 4 4 3 7 2 14 18
Bud 10 1 4 3 1 1 4 4 4 2 1 3 2 4 3 2 1 1 0 0 3 1 6 0
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1
Bud Site 2 0 0 0 0 0 1 0 1 1 0 1 0 0 0 1 0 5 – – – – – –
Cell Periphery 3 5 5 8 3 4 1 2 1 3 1 1 4 7 5 5 2 2 0 0 0 0 0 0
Cytoplasm 1 1 9 15 18 14 20 27 10 14 11 9 3 5 6 5 0 3 0 1 4 0 0 1
Endoplasmic Reticulum 0 0 1 0 0 0 3 2 3 2 1 1 0 0 1 1 0 2 0 0 0 0 0 1
Endosome 4 0 1 4 1 0 3 1 0 1 0 1 2 7 6 4 0 0 5 1 6 2 13 9
Golgi 3 6 39 39 64 10 19 30 5 33 3 14 12 68 93 31 9 2 12 14 10 10 28 25
Mitochondria 293 106 97 91 96 147 225 166 200 166 153 164 150 236 197 11 3 6 259 90 116 121 238 223
Nucleus 1 2 0 0 1 1 0 2 2 0 0 1 0 1 0 3 1 2 0 0 0 0 0 0
Nuclear Periphery 10 1 9 1 4 13 18 11 8 8 3 8 7 7 2 0 0 0 2 0 0 0 4 4
Nucleolus 20 3 6 10 6 19 43 34 34 31 15 21 4 5 3 12 8 13 0 1 0 2 3 5
Peroxisomes 11 0 7 8 2 2 4 3 5 3 3 1 8 25 30 15 3 6 16 1 7 7 23 36
SpindlePole 1 0 2 1 2 0 0 3 3 2 1 1 3 2 5 8 0 0 0 0 0 0 6 3
Vac/Vac Membrane 15 9 8 14 24 19 14 23 15 34 7 11 7 24 19 17 1 1 0 2 1 3 8 7
Unique Cell Count 306 113 139 138 173 195 300 257 245 249 175 199 167 297 267 101 32 39 305 118 167 157 352 341
Labelled Cell Count 374 135 197 199 225 231 357 312 292 302 200 239 206 397 374 123 34 47 305 118 167 157 352 341


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 22.4 15.3 17.4 18.5 20.0 22.0 22.9 20.9 24.2 22.2 24.6 14.8 15.5 15.5 26.2 31.6 30.2 15.8 15.4 16.9
Std Deviation (1e-4) 3.9 6.5 4.7 5.8 5.7 14.3 9.7 10.2 10.4 11.3 10.6 11.6 3.6 4.2 4.0 11.6 10.7 12.7 5.8 5.8 5.6
Intensity Change (Log2) – – – 0.18 0.28 0.39 0.53 0.58 0.45 0.66 0.54 0.69 -0.05 0.02 0.02 0.78 1.05 0.98 0.04 0.02 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 0 0 0 0 0 0 0 0 -1.8 0 0 0.4 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.2 0.3 0.1 1.3 -1.0 -0.3 -0.1 -0.8 -2.1 0 -2.1 -0.5 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 1.7 -5.8 -6.3 -4.1 -7.7 -3.6 -6.8 -5.2 -4.9 -1.2 1.4 0.4 0 -3.0
Mitochondria -0.7 -2.6 1.1 1.1 -1.0 2.7 -0.6 3.9 2.7 4.4 2.2 0.9 -9.1 -6.3 -6.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -1.8 0.1 -0.2 -1.0 -1.5 -1.5 -2.2 -1.0 -0.9 -2.1 0 0 0 0
Nucleolus 1.0 -0.4 1.9 3.1 2.8 2.9 2.6 1.5 2.1 0 0 0 2.2 0 0
Peroxisomes 0.3 0 0 0 0 0 0 0 0 -0.1 1.3 2.1 2.6 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.4 2.3 1.3 -0.5 1.1 0.1 2.4 -0.7 -0.1 -0.6 0.9 0.5 2.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 40.6871 41.0371 42.4609 39.9593 39.8188 41.3703 34.1012 36.4447 33.8177 30.3168 25.8405 35.7082 50.944 49.1104 48.9042 43.3139 54.5552 53.2399
Actin 0.005 0.0012 0.001 0.0001 0.0022 0.0003 0.0082 0.0049 0.0052 0.0002 0.0006 0.0019 0.0104 0.0015 0.0054 0.0036 0.0014 0.0007
Bud 0.0001 0.0001 0 0 0.0001 0.0003 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0 0
Bud Neck 0.0001 0 0 0 0.0002 0 0.0005 0 0.0002 0 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001
Bud Periphery 0.0001 0.0001 0 0 0.0001 0.0004 0.0004 0 0.0003 0 0.0002 0 0.0001 0 0.0002 0.0001 0.0001 0
Bud Site 0.0002 0.0001 0 0 0.0004 0.0001 0.0006 0.0001 0.0007 0 0.0004 0.0001 0.0009 0 0.0003 0.0006 0.0002 0
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0003 0 0 0 0.0006 0 0.0004 0 0.001 0 0.001 0 0.0014 0 0.0003 0.0004 0 0
Cytoplasmic Foci 0.0094 0.0001 0.0016 0.0005 0.0025 0.0001 0.0103 0.0003 0.0067 0.0005 0.0026 0.0029 0.0078 0 0.0069 0.0385 0.0004 0.0032
Eisosomes 0.0006 0.0007 0.0013 0.001 0.0016 0.0003 0.0005 0.0004 0.0007 0.0002 0.0003 0.0019 0.0037 0.0009 0.0008 0.001 0.0007 0.0001
Endoplasmic Reticulum 0.0003 0 0 0 0.0004 0 0.001 0 0.0026 0 0.0011 0 0.0006 0 0.0002 0.0001 0 0
Endosome 0.0022 0.0002 0.0008 0 0.0027 0.0004 0.0185 0.0006 0.0027 0.0004 0.0352 0.0009 0.0033 0.0001 0.0037 0.0437 0.0007 0.0011
Golgi 0.0014 0.0015 0.0009 0 0.0027 0.0063 0.014 0.0102 0.0042 0.0022 0.0173 0.0208 0.0021 0.0018 0.002 0.0141 0.0008 0.0023
Lipid Particles 0.0088 0.004 0.0043 0.0019 0.0176 0.0006 0.0111 0.011 0.009 0.021 0.0131 0.0079 0.014 0.0037 0.0041 0.0226 0.0062 0.0065
Mitochondria 0.9463 0.9864 0.9784 0.9878 0.9153 0.9862 0.9088 0.9638 0.9444 0.9602 0.9025 0.9531 0.9354 0.9868 0.9618 0.8076 0.9833 0.978
None 0.0011 0 0.0004 0 0.0067 0 0.0009 0 0.0028 0 0.0012 0.0001 0.0042 0 0.0007 0.0017 0.0002 0
Nuclear Periphery 0.0043 0 0.0002 0 0.0044 0 0.0036 0.0001 0.0045 0.0001 0.0071 0 0.0025 0 0.0011 0.0002 0.0001 0
Nucleolus 0.0059 0.0002 0.003 0.0046 0.0192 0.0011 0.0003 0.0002 0.0003 0.0014 0.0017 0.0052 0.0018 0.0003 0.0007 0.0105 0.0002 0
Nucleus 0.003 0 0.0003 0.0001 0.0024 0.0002 0.0004 0 0.0004 0.0002 0.0026 0.0004 0.0004 0 0.0012 0.0007 0.0001 0
Peroxisomes 0.0038 0.0007 0.001 0.0021 0.0025 0.0004 0.0148 0.0026 0.0044 0.0018 0.0019 0.0022 0.0054 0.0005 0.0044 0.0497 0.0043 0.0059
Punctate Nuclear 0.0008 0 0.0004 0 0.011 0 0.0005 0 0.001 0 0.003 0.0005 0.0016 0 0.0005 0.0021 0.0001 0.0001
Vacuole 0.0008 0.0006 0.0003 0.0002 0.0005 0.0012 0.0015 0.0005 0.0018 0.001 0.0037 0.0002 0.0008 0.0003 0.0033 0.0009 0.0002 0.0001
Vacuole Periphery 0.0055 0.004 0.0059 0.0015 0.0067 0.0018 0.0036 0.0051 0.0063 0.0104 0.0043 0.0015 0.0034 0.0038 0.0021 0.0014 0.001 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.8749 90.2951 65.5463 91.2894 81.1392 95.4657 102.9576 101.4713 111.1787 95.4944
Translational Efficiency 1.9957 1.4588 1.8989 1.7828 1.9335 1.9229 1.7321 2.3457 2.1196 1.9364

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
Localization
Cell Percentages mitochondrion (100%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mdh1

Mdh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available