Standard name | |
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Human Ortholog | |
Description | GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 10 | 31 | 24 | 36 | 45 | 32 | 36 | 19 | – | 20 | 17 | 36 | 76 | 68 | 3 | 8 | 7 | 9 | 8 | 30 | 37 | 61 | 56 |
Bud | 9 | 7 | 30 | 31 | 48 | 50 | 46 | 57 | 42 | – | 66 | 63 | 18 | 36 | 39 | 2 | 6 | 3 | 5 | 5 | 17 | 27 | 61 | 86 |
Bud Neck | 3 | 1 | 16 | 16 | 39 | 27 | 35 | 30 | 18 | – | 32 | 30 | 14 | 15 | 23 | 0 | 3 | 0 | 26 | 32 | 63 | 74 | 121 | 113 |
Bud Site | 1 | 0 | 3 | 19 | 28 | 35 | 24 | 27 | 24 | – | 32 | 49 | 4 | 15 | 31 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 22 | 27 | 55 | 45 | 14 | 25 | 27 | 19 | 26 | – | 21 | 26 | 32 | 22 | 24 | 2 | 5 | 4 | 11 | 15 | 53 | 10 | 6 | 5 |
Cytoplasm | 77 | 128 | 262 | 136 | 123 | 120 | 140 | 137 | 83 | – | 78 | 72 | 166 | 118 | 116 | 9 | 16 | 32 | 44 | 91 | 132 | 23 | 17 | 7 |
Endoplasmic Reticulum | 3 | 2 | 6 | 3 | 7 | 8 | 11 | 9 | 3 | – | 3 | 5 | 2 | 3 | 2 | 2 | 2 | 4 | 2 | 2 | 7 | 0 | 2 | 2 |
Endosome | 1 | 1 | 0 | 2 | 5 | 2 | 2 | 4 | 0 | – | 0 | 0 | 5 | 4 | 6 | 0 | 1 | 4 | 0 | 1 | 7 | 1 | 3 | 2 |
Golgi | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 0 | 4 | 6 | 23 | 17 | 58 | 80 | 110 | 101 | – | 159 | 164 | 1 | 1 | 5 | 1 | 1 | 3 | 1 | 5 | 16 | 6 | 5 | 7 |
Nucleus | 0 | 0 | 0 | 1 | 5 | 3 | 13 | 7 | 6 | – | 8 | 12 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | – | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 8 | 6 | 9 | 12 | – | 8 | 8 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 4 | 9 | 20 | 26 | 25 | 10 | – | 20 | 35 | 0 | 7 | 17 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 2 | 0 | 12 | 14 | 38 | 24 | 54 | 53 | 21 | – | 32 | 45 | 13 | 14 | 16 | 2 | 10 | 8 | 0 | 0 | 3 | 0 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 1 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 1 |
Unique Cell Count | 97 | 147 | 324 | 222 | 277 | 302 | 358 | 367 | 257 | 329 | 352 | 227 | 224 | 246 | 16 | 39 | 58 | 108 | 167 | 342 | 186 | 290 | 288 | |
Labelled Cell Count | 130 | 181 | 424 | 318 | 375 | 425 | 501 | 526 | 366 | 479 | 529 | 291 | 313 | 348 | 23 | 55 | 76 | 108 | 167 | 342 | 186 | 290 | 288 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 5.9 | 5.0 | 4.9 | 5.2 | 4.8 | 4.7 | 4.6 | 4.2 | – | 4.0 | 3.8 | 5.6 | 5.7 | 5.0 | 7.4 | 8.1 | 8.3 | 5.9 | 5.8 | 6.4 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.1 | 1.5 | 1.9 | 1.3 | 1.4 | 1.5 | 1.7 | – | 1.3 | 1.1 | 0.9 | 0.7 | 0.9 | 0.9 | 1.9 | 2.3 | 1.6 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.06 | -0.05 | -0.08 | -0.11 | -0.26 | – | -0.31 | -0.38 | 0.17 | 0.19 | 0.0 | 0.58 | 0.7 | 0.74 | 0.25 | 0.22 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | 1.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2.2 | 7.1 | 5.6 | 0 | 0 | 0.6 |
Bud | 1.7 | 2.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.5 | 2.4 | 2.4 | 0 | 0 | -1.0 |
Bud Neck | 1.1 | 3.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.6 | 0.9 | 2.1 | 0 | 0 | 0 |
Bud Site | 4.5 | 5.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.7 | 5.8 | 0 | 0 | 0 |
Cell Periphery | 1.0 | -4.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.9 | -2.4 | -2.5 | 0 | -0.7 | -2.0 |
Cytoplasm | -5.1 | -9.3 | -10.5 | -11.1 | -11.6 | -11.8 | – | -14.6 | -15.7 | -2.1 | -7.0 | -8.4 | -2.4 | -5.6 | -4.3 |
Endoplasmic Reticulum | 0 | 0.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | 2.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 3.5 | 0 | 0 | – | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4.5 | 0 | 0 | 0 |
SpindlePole | 1.4 | 4.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.1 | 1.4 | 1.5 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1092 | 3.2823 | 3.4387 | 3.1495 | 3.2644 | 3.3444 | 4.2301 | 4.7654 | 4.7304 | 5.0942 | 4.3769 | 4.508 | 4.3181 | 5.2113 | 4.688 | 4.7056 | 4.0988 | 4.788 |
Actin | 0.0605 | 0.0059 | 0.0352 | 0.0104 | 0.0221 | 0.0267 | 0.019 | 0.0091 | 0.04 | 0.0065 | 0.0486 | 0.0096 | 0.0253 | 0.0131 | 0.0307 | 0.0037 | 0.0163 | 0.0107 |
Bud | 0.0039 | 0.0025 | 0.0025 | 0.0018 | 0.0028 | 0.0033 | 0.008 | 0.0094 | 0.0073 | 0.0117 | 0.007 | 0.0062 | 0.0014 | 0.0031 | 0.0027 | 0.0007 | 0.0008 | 0.0009 |
Bud Neck | 0.0277 | 0.014 | 0.4354 | 0.4908 | 0.3005 | 0.124 | 0.0199 | 0.0072 | 0.3595 | 0.5121 | 0.2117 | 0.0912 | 0.04 | 0.002 | 0.3684 | 0.6535 | 0.2655 | 0.1308 |
Bud Periphery | 0.0076 | 0.0032 | 0.0031 | 0.0044 | 0.0067 | 0.0107 | 0.0187 | 0.0078 | 0.0096 | 0.0285 | 0.0147 | 0.0158 | 0.004 | 0.0051 | 0.0068 | 0.001 | 0.0013 | 0.0037 |
Bud Site | 0.1575 | 0.183 | 0.1314 | 0.0145 | 0.0097 | 0.0143 | 0.1509 | 0.1746 | 0.1856 | 0.0322 | 0.0286 | 0.0074 | 0.1671 | 0.1156 | 0.1516 | 0.0484 | 0.0194 | 0.0033 |
Cell Periphery | 0.0306 | 0.0159 | 0.0065 | 0.0057 | 0.0044 | 0.0098 | 0.0229 | 0.0138 | 0.013 | 0.0071 | 0.006 | 0.0072 | 0.0515 | 0.0131 | 0.0139 | 0.0021 | 0.0037 | 0.0092 |
Cytoplasm | 0.399 | 0.6348 | 0.2591 | 0.3526 | 0.5092 | 0.6696 | 0.5083 | 0.6476 | 0.2565 | 0.231 | 0.4869 | 0.685 | 0.5836 | 0.7243 | 0.3381 | 0.2739 | 0.6236 | 0.7171 |
Cytoplasmic Foci | 0.0207 | 0.0097 | 0.0279 | 0.0057 | 0.0073 | 0.0145 | 0.02 | 0.007 | 0.0244 | 0.0339 | 0.0134 | 0.0124 | 0.0103 | 0.0019 | 0.0115 | 0.0055 | 0.0251 | 0.0048 |
Eisosomes | 0.0017 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0008 | 0.0003 | 0.0008 | 0.0001 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0097 | 0.0041 | 0.003 | 0.005 | 0.0035 | 0.0026 | 0.0073 | 0.002 | 0.0016 | 0.0014 | 0.0023 | 0.0024 | 0.0067 | 0.0072 | 0.0018 | 0.0018 | 0.0022 | 0.0033 |
Endosome | 0.044 | 0.0039 | 0.0079 | 0.0353 | 0.0083 | 0.0082 | 0.0146 | 0.0013 | 0.0115 | 0.0464 | 0.0243 | 0.0048 | 0.0152 | 0.0005 | 0.0073 | 0.0014 | 0.0034 | 0.0007 |
Golgi | 0.0179 | 0.0022 | 0.0047 | 0.0044 | 0.0073 | 0.0037 | 0.0025 | 0.0008 | 0.006 | 0.0061 | 0.0156 | 0.0051 | 0.0022 | 0.0004 | 0.0013 | 0.001 | 0.0028 | 0.0004 |
Lipid Particles | 0.0356 | 0.0008 | 0.0047 | 0.012 | 0.0094 | 0.0043 | 0.0102 | 0.0007 | 0.005 | 0.0127 | 0.0158 | 0.0031 | 0.0046 | 0.0004 | 0.0031 | 0.0001 | 0.0014 | 0.0007 |
Mitochondria | 0.0306 | 0.0002 | 0.0025 | 0.0112 | 0.0479 | 0.0016 | 0.0026 | 0.0004 | 0.0032 | 0.0033 | 0.0105 | 0.0046 | 0.0014 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 |
None | 0.0741 | 0.1163 | 0.0329 | 0.0267 | 0.0395 | 0.0793 | 0.1488 | 0.1097 | 0.0574 | 0.0438 | 0.0713 | 0.1299 | 0.0742 | 0.1108 | 0.05 | 0.0048 | 0.028 | 0.1118 |
Nuclear Periphery | 0.024 | 0.0003 | 0.0079 | 0.0015 | 0.0004 | 0.0036 | 0.0132 | 0.0006 | 0.0007 | 0.0009 | 0.0011 | 0.0014 | 0.0014 | 0.0004 | 0.0012 | 0.0001 | 0.0003 | 0.0002 |
Nucleolus | 0.0064 | 0.0002 | 0.0008 | 0.0007 | 0.0003 | 0.0023 | 0.0015 | 0.0006 | 0.0007 | 0.0027 | 0.0025 | 0.0003 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0006 | 0.0001 |
Nucleus | 0.0114 | 0.0009 | 0.0058 | 0.0024 | 0.0009 | 0.0026 | 0.0127 | 0.0038 | 0.0032 | 0.0015 | 0.0023 | 0.002 | 0.0009 | 0.0006 | 0.0018 | 0.0006 | 0.0011 | 0.0005 |
Peroxisomes | 0.0178 | 0.0002 | 0.0221 | 0.0017 | 0.013 | 0.0089 | 0.0032 | 0.0003 | 0.0097 | 0.0035 | 0.0178 | 0.0078 | 0.0032 | 0.0001 | 0.0018 | 0.0001 | 0.0009 | 0.0002 |
Punctate Nuclear | 0.0072 | 0.0003 | 0.0032 | 0.0005 | 0.0003 | 0.0059 | 0.0034 | 0.0012 | 0.0024 | 0.002 | 0.011 | 0.0009 | 0.0017 | 0.0002 | 0.0021 | 0.0002 | 0.0023 | 0.0003 |
Vacuole | 0.0086 | 0.0014 | 0.0023 | 0.0101 | 0.0055 | 0.0028 | 0.0103 | 0.0019 | 0.0019 | 0.0102 | 0.0068 | 0.0026 | 0.004 | 0.0007 | 0.0033 | 0.0007 | 0.001 | 0.0011 |
Vacuole Periphery | 0.0033 | 0.0001 | 0.0007 | 0.0027 | 0.0007 | 0.0006 | 0.0017 | 0.0001 | 0.0002 | 0.002 | 0.0016 | 0.0004 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.644 | 10.1167 | 5.5199 | 12.7689 | 15.7518 | 9.6474 | 12.0341 | 9.1375 | 10.7583 | 20.1735 |
Translational Efficiency | 1.0099 | 0.7517 | 0.8923 | 0.5451 | 0.6568 | 1.3355 | 0.6196 | 0.4871 | 0.5853 | 0.5434 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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