Standard name
Human Ortholog
Description GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.07 0.1 0.11 0.13 0.15 0.09 0.1 0.07 0.06 0.05 0.16 0.34 0.28 0.19 0.21 0.12 0.09 0.05 0.09 0.2 0.21 0.19
Bud 0.09 0.05 0.09 0.14 0.17 0.17 0.13 0.16 0.16 0.2 0.18 0.08 0.16 0.16 0.12 0.15 0.05 0.05 0 0.05 0.15 0.21 0.3
Bud Neck 0 0 0.05 0.07 0.14 0.09 0.1 0.08 0.07 0.1 0.09 0.06 0.07 0.09 0 0.08 0 0.25 0.2 0.19 0.4 0.42 0.4
Bud Site 0 0 0 0.09 0.1 0.12 0.07 0.07 0.09 0.1 0.14 0 0.07 0.13 0.06 0 0
Cell Periphery 0.23 0.18 0.17 0.2 0.05 0.08 0.08 0.05 0.1 0.06 0.07 0.14 0.1 0.1 0.12 0.13 0.07 0.11 0.09 0.16 0.06 0 0
Cytoplasm 0.79 0.87 0.81 0.61 0.44 0.4 0.39 0.37 0.32 0.24 0.2 0.73 0.53 0.47 0.56 0.41 0.55 0.42 0.55 0.39 0.12 0.06 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.06 0.19 0.22 0.3 0.39 0.48 0.47 0 0 0 0.06 0 0.05 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.07 0.07 0.07 0 0.06 0.1 0 0 0.07 0.06 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.14 0.08 0.15 0.14 0.08 0.1 0.13 0.06 0.06 0.07 0.12 0.26 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 10 31 24 36 45 32 36 19 20 17 36 76 68 3 8 7 9 8 30 37 61 56
Bud 9 7 30 31 48 50 46 57 42 66 63 18 36 39 2 6 3 5 5 17 27 61 86
Bud Neck 3 1 16 16 39 27 35 30 18 32 30 14 15 23 0 3 0 26 32 63 74 121 113
Bud Site 1 0 3 19 28 35 24 27 24 32 49 4 15 31 1 1 2
Cell Periphery 22 27 55 45 14 25 27 19 26 21 26 32 22 24 2 5 4 11 15 53 10 6 5
Cytoplasm 77 128 262 136 123 120 140 137 83 78 72 166 118 116 9 16 32 44 91 132 23 17 7
Endoplasmic Reticulum 3 2 6 3 7 8 11 9 3 3 5 2 3 2 2 2 4 2 2 7 0 2 2
Endosome 1 1 0 2 5 2 2 4 0 0 0 5 4 6 0 1 4 0 1 7 1 3 2
Golgi 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 0 0 0
Mitochondria 0 4 6 23 17 58 80 110 101 159 164 1 1 5 1 1 3 1 5 16 6 5 7
Nucleus 0 0 0 1 5 3 13 7 6 8 12 0 0 0 0 0 4 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 1 1 0 0 1 0 1 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 3 8 6 9 12 8 8 0 1 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 1 4 9 20 26 25 10 20 35 0 7 17 1 0 3 0 0 0 0 1 0
SpindlePole 2 0 12 14 38 24 54 53 21 32 45 13 14 16 2 10 8 0 0 3 0 1 1
Vac/Vac Membrane 0 0 0 0 1 0 4 2 1 0 2 0 0 0 0 0 2 0 2 3 1 2 1
Unique Cell Count 97 147 324 222 277 302 358 367 257 329 352 227 224 246 16 39 58 108 167 342 186 290 288
Labelled Cell Count 130 181 424 318 375 425 501 526 366 479 529 291 313 348 23 55 76 108 167 342 186 290 288


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 5.9 5.0 4.9 5.2 4.8 4.7 4.6 4.2 4.0 3.8 5.6 5.7 5.0 7.4 8.1 8.3 5.9 5.8 6.4
Std Deviation (1e-4) 0.7 1.0 1.1 1.5 1.9 1.3 1.4 1.5 1.7 1.3 1.1 0.9 0.7 0.9 0.9 1.9 2.3 1.6 1.3 1.3
Intensity Change (Log2) -0.02 0.06 -0.05 -0.08 -0.11 -0.26 -0.31 -0.38 0.17 0.19 0.0 0.58 0.7 0.74 0.25 0.22 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 1.3 0 0 0 0 0 0 2.2 7.1 5.6 0 0 0.6
Bud 1.7 2.9 0 0 0 0 0 0 -0.5 2.4 2.4 0 0 -1.0
Bud Neck 1.1 3.9 0 0 0 0 0 0 0.6 0.9 2.1 0 0 0
Bud Site 4.5 5.1 0 0 0 0 0 0 0 3.7 5.8 0 0 0
Cell Periphery 1.0 -4.6 0 0 0 0 0 0 -0.9 -2.4 -2.5 0 -0.7 -2.0
Cytoplasm -5.1 -9.3 -10.5 -11.1 -11.6 -11.8 -14.6 -15.7 -2.1 -7.0 -8.4 -2.4 -5.6 -4.3
Endoplasmic Reticulum 0 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.4 2.7 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 3.5 0 0 0 3.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 4.5 0 0 0
SpindlePole 1.4 4.4 0 0 0 0 0 0 1.1 1.4 1.5 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1092 3.2823 3.4387 3.1495 3.2644 3.3444 4.2301 4.7654 4.7304 5.0942 4.3769 4.508 4.3181 5.2113 4.688 4.7056 4.0988 4.788
Actin 0.0605 0.0059 0.0352 0.0104 0.0221 0.0267 0.019 0.0091 0.04 0.0065 0.0486 0.0096 0.0253 0.0131 0.0307 0.0037 0.0163 0.0107
Bud 0.0039 0.0025 0.0025 0.0018 0.0028 0.0033 0.008 0.0094 0.0073 0.0117 0.007 0.0062 0.0014 0.0031 0.0027 0.0007 0.0008 0.0009
Bud Neck 0.0277 0.014 0.4354 0.4908 0.3005 0.124 0.0199 0.0072 0.3595 0.5121 0.2117 0.0912 0.04 0.002 0.3684 0.6535 0.2655 0.1308
Bud Periphery 0.0076 0.0032 0.0031 0.0044 0.0067 0.0107 0.0187 0.0078 0.0096 0.0285 0.0147 0.0158 0.004 0.0051 0.0068 0.001 0.0013 0.0037
Bud Site 0.1575 0.183 0.1314 0.0145 0.0097 0.0143 0.1509 0.1746 0.1856 0.0322 0.0286 0.0074 0.1671 0.1156 0.1516 0.0484 0.0194 0.0033
Cell Periphery 0.0306 0.0159 0.0065 0.0057 0.0044 0.0098 0.0229 0.0138 0.013 0.0071 0.006 0.0072 0.0515 0.0131 0.0139 0.0021 0.0037 0.0092
Cytoplasm 0.399 0.6348 0.2591 0.3526 0.5092 0.6696 0.5083 0.6476 0.2565 0.231 0.4869 0.685 0.5836 0.7243 0.3381 0.2739 0.6236 0.7171
Cytoplasmic Foci 0.0207 0.0097 0.0279 0.0057 0.0073 0.0145 0.02 0.007 0.0244 0.0339 0.0134 0.0124 0.0103 0.0019 0.0115 0.0055 0.0251 0.0048
Eisosomes 0.0017 0.0002 0.0005 0.0001 0.0003 0.0006 0.0004 0.0003 0.0005 0.0004 0.0003 0.0001 0.0008 0.0003 0.0008 0.0001 0.0003 0.0003
Endoplasmic Reticulum 0.0097 0.0041 0.003 0.005 0.0035 0.0026 0.0073 0.002 0.0016 0.0014 0.0023 0.0024 0.0067 0.0072 0.0018 0.0018 0.0022 0.0033
Endosome 0.044 0.0039 0.0079 0.0353 0.0083 0.0082 0.0146 0.0013 0.0115 0.0464 0.0243 0.0048 0.0152 0.0005 0.0073 0.0014 0.0034 0.0007
Golgi 0.0179 0.0022 0.0047 0.0044 0.0073 0.0037 0.0025 0.0008 0.006 0.0061 0.0156 0.0051 0.0022 0.0004 0.0013 0.001 0.0028 0.0004
Lipid Particles 0.0356 0.0008 0.0047 0.012 0.0094 0.0043 0.0102 0.0007 0.005 0.0127 0.0158 0.0031 0.0046 0.0004 0.0031 0.0001 0.0014 0.0007
Mitochondria 0.0306 0.0002 0.0025 0.0112 0.0479 0.0016 0.0026 0.0004 0.0032 0.0033 0.0105 0.0046 0.0014 0.0001 0.0004 0.0002 0.0002 0.0001
None 0.0741 0.1163 0.0329 0.0267 0.0395 0.0793 0.1488 0.1097 0.0574 0.0438 0.0713 0.1299 0.0742 0.1108 0.05 0.0048 0.028 0.1118
Nuclear Periphery 0.024 0.0003 0.0079 0.0015 0.0004 0.0036 0.0132 0.0006 0.0007 0.0009 0.0011 0.0014 0.0014 0.0004 0.0012 0.0001 0.0003 0.0002
Nucleolus 0.0064 0.0002 0.0008 0.0007 0.0003 0.0023 0.0015 0.0006 0.0007 0.0027 0.0025 0.0003 0.0002 0.0001 0.0011 0.0001 0.0006 0.0001
Nucleus 0.0114 0.0009 0.0058 0.0024 0.0009 0.0026 0.0127 0.0038 0.0032 0.0015 0.0023 0.002 0.0009 0.0006 0.0018 0.0006 0.0011 0.0005
Peroxisomes 0.0178 0.0002 0.0221 0.0017 0.013 0.0089 0.0032 0.0003 0.0097 0.0035 0.0178 0.0078 0.0032 0.0001 0.0018 0.0001 0.0009 0.0002
Punctate Nuclear 0.0072 0.0003 0.0032 0.0005 0.0003 0.0059 0.0034 0.0012 0.0024 0.002 0.011 0.0009 0.0017 0.0002 0.0021 0.0002 0.0023 0.0003
Vacuole 0.0086 0.0014 0.0023 0.0101 0.0055 0.0028 0.0103 0.0019 0.0019 0.0102 0.0068 0.0026 0.004 0.0007 0.0033 0.0007 0.001 0.0011
Vacuole Periphery 0.0033 0.0001 0.0007 0.0027 0.0007 0.0006 0.0017 0.0001 0.0002 0.002 0.0016 0.0004 0.0005 0 0.0003 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.644 10.1167 5.5199 12.7689 15.7518 9.6474 12.0341 9.1375 10.7583 20.1735
Translational Efficiency 1.0099 0.7517 0.8923 0.5451 0.6568 1.3355 0.6196 0.4871 0.5853 0.5434

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains
Localization
Cell Percentages cytoplasm (45%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Bud2

Bud2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available