Standard name
Human Ortholog
Description Monoglyceride lipase (MGL); functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.26 0.12 0.13 0.26 0.1 0.08 0.2 0.2 0.14 0.14 0.1 0.17 0.22 0.15 0.11 0.09 0.06 0.11 0.08 0.09 0.1 0.13 0.18 0.17
Bud 0.06 0.05 0.05 0 0 0 0 0.06 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.08 0.08 0 0.1 0.07 0 0 0 0 0 0.05 0 0.06 0.06 0.22 0.22 0.36 0 0 0 0 0 0
Cytoplasm 0.09 0.19 0.12 0.15 0.27 0.33 0.32 0.26 0.38 0.33 0.32 0.38 0.1 0.06 0.11 0.5 0.56 0.47 0 0.09 0 0 0 0
Endoplasmic Reticulum 0.69 0.72 0.75 0.68 0.63 0.65 0.47 0.46 0.26 0.4 0.26 0.3 0.48 0.62 0.6 0 0 0.05 0.81 0.67 0.66 0.69 0.62 0.55
Endosome 0.08 0 0 0 0 0.05 0 0.1 0.08 0 0 0.07 0.06 0 0.08 0 0 0 0 0 0.07 0.05 0.05 0.09
Golgi 0 0 0 0.13 0 0 0 0 0 0 0 0 0.16 0.09 0.05 0.14 0.16 0.06 0 0 0 0 0 0.09
Mitochondria 0 0 0 0 0 0 0.11 0.11 0.27 0.18 0.36 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.18 0.13 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 49 21 8 34 25 19 59 49 22 26 24 37 54 60 48 11 2 15 16 19 10 28 78 63
Bud 11 9 3 5 6 3 11 15 9 8 22 7 1 1 4 3 0 4 0 2 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 7 1 1 6 4
Bud Site 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 14 5 5 27 15 13 4 0 4 6 10 6 23 26 26 7 48 1 1 1 2 3 2
Cytoplasm 18 34 7 20 70 76 96 65 60 61 74 84 24 25 48 60 18 63 1 18 3 7 8 3
Endoplasmic Reticulum 132 129 45 90 165 150 141 115 41 74 59 67 119 257 274 1 1 7 172 144 71 147 269 212
Endosome 15 1 0 2 3 11 11 26 12 6 9 16 14 15 38 4 1 0 3 6 7 10 22 35
Golgi 6 4 1 17 7 0 8 8 1 1 0 2 40 37 22 17 5 8 2 3 2 5 18 33
Mitochondria 8 0 1 4 0 3 34 28 43 33 83 36 0 0 0 3 0 3 3 1 2 3 8 8
Nucleus 1 1 0 0 0 1 1 1 3 2 3 1 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 0 0 1 1 0 0 0 1 0 2 0 0 1
Nucleolus 1 0 0 1 0 0 1 4 3 0 1 0 3 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 2
SpindlePole 7 1 2 2 5 0 4 4 0 1 1 2 36 74 60 0 0 2 0 0 0 0 1 0
Vac/Vac Membrane 1 0 0 3 1 0 8 6 5 12 5 11 3 4 8 4 2 0 2 6 1 3 6 9
Unique Cell Count 190 179 60 133 262 230 301 249 159 186 229 221 247 412 454 119 32 133 213 215 108 212 431 384
Labelled Cell Count 252 214 72 183 309 278 388 326 199 229 288 274 301 498 529 129 36 150 213 215 108 212 431 384


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 14.8 12.0 11.5 11.8 9.9 9.4 9.4 7.8 8.4 7.7 8.0 12.7 13.5 13.4 16.9 20.2 20.0 13.7 13.0 14.1
Std Deviation (1e-4) 1.8 2.3 1.9 2.0 1.8 1.8 1.7 1.8 1.5 1.7 1.5 1.5 1.8 2.1 2.0 3.4 5.8 4.7 8.4 1.9 2.0
Intensity Change (Log2) – – – -0.07 -0.03 -0.27 -0.36 -0.35 -0.63 -0.52 -0.64 -0.59 0.08 0.17 0.16 0.49 0.75 0.74 0.18 0.11 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.9 -0.9 -1.2 1.1 1.1 0.1 0.1 -0.6 0.6 1.5 0.3 -0.6 -0.8 0 -0.4
Bud 0 0 0 0 0 0 0 1.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.5 0 0 0 0 0 0 0 0 0 0 2.3 0 4.0
Cytoplasm 0.6 2.5 3.3 3.2 2.4 3.7 3.2 3.2 3.9 -0.4 0 -0.3 5.1 4.6 4.8
Endoplasmic Reticulum -1.0 -1.8 -1.4 -4.0 -4.0 -6.7 -4.7 -7.1 -6.3 -3.7 -1.9 -2.2 -10.7 -6.6 -10.1
Endosome 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0
Golgi 2.5 0 0 0 0 0 0 0 0 3.0 0 0 2.7 0 0
Mitochondria 0 0 0 2.3 2.3 4.2 3.1 5.3 3.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2.4 2.9 2.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1672 9.4298 9.1167 8.1328 8.9683 9.2428 6.6768 9.2233 8.2466 7.8175 7.4966 9.1005 11.4101 11.6751 11.8219 11.2454 10.5882 11.5914
Actin 0.0078 0.0072 0.0021 0.0152 0.0068 0.0044 0.0219 0.0479 0.0317 0.0619 0.0542 0.0247 0.0273 0.0069 0.0125 0.0112 0.0353 0.0081
Bud 0.0001 0.0002 0.0001 0.0001 0.0008 0.0001 0.0005 0.0011 0.0008 0.0007 0.0009 0.0005 0.0017 0.0003 0.0002 0.0002 0.0003 0.0003
Bud Neck 0.0005 0.003 0.001 0.002 0.0039 0.0055 0.0151 0.0126 0.0155 0.0199 0.0732 0.0224 0.0029 0.0021 0.0051 0.0024 0.0052 0.0033
Bud Periphery 0.0002 0.0003 0.0002 0.0001 0.0014 0.0003 0.0009 0.0015 0.002 0.0027 0.0031 0.0015 0.0012 0.0005 0.0005 0.0012 0.0031 0.0004
Bud Site 0.0005 0.0076 0.0004 0.0004 0.0111 0.0002 0.0119 0.0663 0.0236 0.0028 0.0021 0.0017 0.0097 0.0092 0.0032 0.0004 0.0008 0.0005
Cell Periphery 0.0111 0.0198 0.0117 0.0081 0.0087 0.0071 0.024 0.0285 0.0531 0.0404 0.0213 0.0312 0.023 0.0114 0.0176 0.0465 0.0578 0.0097
Cytoplasm 0.0177 0.0276 0.0196 0.011 0.0143 0.0395 0.0721 0.0924 0.1011 0.0694 0.1664 0.0668 0.0476 0.0338 0.0252 0.0277 0.0319 0.0313
Cytoplasmic Foci 0.0316 0.0365 0.0295 0.0255 0.066 0.0309 0.0804 0.0752 0.0591 0.054 0.0391 0.0436 0.0302 0.0315 0.0286 0.0105 0.0265 0.0312
Eisosomes 0.0002 0.0007 0.0002 0.0003 0.0001 0.0001 0.0008 0.0007 0.0012 0.0011 0.0006 0.0007 0.0031 0.0003 0.0002 0.001 0.0015 0.0003
Endoplasmic Reticulum 0.1229 0.1313 0.1183 0.2632 0.1501 0.1688 0.0484 0.044 0.0637 0.1299 0.0569 0.088 0.1278 0.1593 0.1637 0.1784 0.0929 0.1627
Endosome 0.04 0.0609 0.0584 0.0301 0.0492 0.0436 0.1084 0.0635 0.0534 0.0623 0.0666 0.0358 0.0283 0.0445 0.0368 0.0104 0.0336 0.0177
Golgi 0.0183 0.0251 0.0284 0.031 0.0492 0.0272 0.0632 0.0737 0.0401 0.0477 0.077 0.0492 0.0209 0.0347 0.0318 0.0201 0.0485 0.0202
Lipid Particles 0.7117 0.6539 0.7157 0.5985 0.6255 0.661 0.4837 0.459 0.5029 0.4664 0.4257 0.6063 0.6485 0.6519 0.6535 0.6791 0.6229 0.7058
Mitochondria 0.001 0.0004 0.0008 0.0003 0.0004 0.0007 0.014 0.0086 0.0106 0.0041 0.0032 0.0028 0.0011 0.001 0.0003 0.0003 0.0021 0.0004
None 0.001 0.0003 0.0002 0.0002 0.0001 0.0002 0.0159 0.0007 0.0103 0.0008 0.0003 0.0046 0.0048 0.0003 0.0002 0.0002 0.0013 0.0009
Nuclear Periphery 0.014 0.0027 0.0014 0.0018 0.0012 0.0023 0.0035 0.001 0.0015 0.0033 0.0006 0.0015 0.0035 0.002 0.0025 0.0018 0.0113 0.0014
Nucleolus 0.0002 0.0005 0.0001 0 0.0003 0 0.001 0.0006 0.0014 0.0004 0.0001 0.0006 0.0004 0.0002 0.0002 0.0001 0.0006 0.0001
Nucleus 0.0028 0.0011 0.0001 0.0001 0.0004 0.0002 0.0018 0.0007 0.0012 0.0008 0.0005 0.0011 0.0013 0.0003 0.0003 0.0002 0.001 0.0003
Peroxisomes 0.0022 0.0008 0.0006 0.0011 0.0014 0.0009 0.0114 0.0045 0.0074 0.003 0.0013 0.0039 0.0026 0.0006 0.0005 0.0002 0.0028 0.0005
Punctate Nuclear 0.0011 0.001 0.0004 0.0004 0.0008 0.0004 0.0036 0.0012 0.0027 0.0015 0.0005 0.0034 0.0047 0.0005 0.0043 0.0002 0.0048 0.0009
Vacuole 0.0144 0.0187 0.0101 0.0103 0.0078 0.006 0.0154 0.0158 0.016 0.0256 0.0057 0.0092 0.0089 0.0082 0.0122 0.0078 0.0147 0.0038
Vacuole Periphery 0.0007 0.0005 0.0006 0.0004 0.0004 0.0006 0.0019 0.0005 0.0007 0.0013 0.0006 0.0006 0.0004 0.0004 0.0004 0.0003 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.3116 61.7794 31.857 50.7758 47.0796 40.9743 48.9663 56.4512 55.3906 54.6146
Translational Efficiency 1.1704 0.8042 1.1859 0.6543 0.8942 1.0823 1.0668 0.9268 1.0213 0.9517

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Monoglyceride lipase (MGL); functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
Localization
Cell Percentages ER (83%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Yju3

Yju3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yju3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available