Standard name | |
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Human Ortholog | |
Description | Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 7 | 2 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 5 | 2 | 3 | 1 | 1 | 1 | 3 | 3 | 7 | 3 | 7 | 9 |
Bud | 7 | 6 | 15 | 7 | 8 | 20 | 23 | 19 | 23 | 17 | 16 | 16 | 1 | 4 | 7 | 9 | 8 | 8 | 0 | 2 | 0 | 2 | 4 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 7 | 6 | 10 | 6 | 11 | 5 | 8 | 0 | 0 | 0 | 2 | 1 | 5 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 9 | 3 | 1 | 1 | 2 | 2 | 4 | 4 | 2 | 6 | 7 | 13 | 18 | 15 | 16 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 2 | 5 | 9 | 11 | 11 | 12 | 15 | 7 | 12 | 12 | 6 | 3 | 8 | 10 | 20 | 12 | 15 | 0 | 0 | 0 | 0 | 2 | 3 |
Endoplasmic Reticulum | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 3 | 2 | 2 | 3 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 2 | 15 | 5 | 10 | 11 | 2 | 9 | 1 | 0 | 1 | 2 | 14 | 26 | 32 | 11 | 29 | 22 | 4 | 13 | 13 | 5 | 16 | 9 |
Golgi | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 60 | 136 | 98 | 24 | 22 | 8 | 9 | 14 | 22 | 18 | 40 | 36 |
Mitochondria | 234 | 219 | 313 | 163 | 260 | 492 | 460 | 456 | 280 | 601 | 339 | 548 | 204 | 222 | 332 | 16 | 8 | 8 | 199 | 191 | 257 | 219 | 256 | 229 |
Nucleus | 0 | 2 | 2 | 0 | 1 | 7 | 4 | 6 | 0 | 6 | 2 | 1 | 0 | 1 | 1 | 3 | 6 | 14 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 15 | 9 | 21 | 6 | 22 | 52 | 49 | 45 | 26 | 62 | 31 | 65 | 10 | 7 | 5 | 7 | 11 | 13 | 1 | 1 | 1 | 1 | 3 | 2 |
Nucleolus | 13 | 7 | 8 | 9 | 20 | 56 | 55 | 75 | 36 | 52 | 31 | 40 | 3 | 5 | 4 | 7 | 14 | 11 | 0 | 0 | 0 | 1 | 3 | 1 |
Peroxisomes | 2 | 7 | 60 | 19 | 11 | 9 | 11 | 31 | 7 | 11 | 3 | 5 | 8 | 9 | 45 | 34 | 44 | 36 | 13 | 9 | 48 | 8 | 16 | 17 |
SpindlePole | 1 | 3 | 13 | 0 | 6 | 6 | 7 | 31 | 14 | 14 | 4 | 11 | 2 | 11 | 12 | 10 | 24 | 23 | 0 | 0 | 1 | 1 | 2 | 0 |
Vac/Vac Membrane | 25 | 20 | 9 | 3 | 15 | 13 | 13 | 13 | 7 | 23 | 7 | 14 | 30 | 61 | 49 | 27 | 36 | 30 | 3 | 7 | 0 | 10 | 20 | 8 |
Unique Cell Count | 249 | 236 | 352 | 184 | 296 | 545 | 512 | 550 | 314 | 656 | 372 | 580 | 251 | 358 | 440 | 157 | 182 | 159 | 239 | 248 | 358 | 276 | 383 | 323 |
Labelled Cell Count | 311 | 286 | 480 | 227 | 367 | 686 | 647 | 716 | 412 | 814 | 453 | 723 | 350 | 507 | 618 | 189 | 236 | 206 | 239 | 248 | 358 | 276 | 383 | 323 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 7.8 | 8.1 | 8.4 | 7.8 | 6.9 | 6.6 | 6.9 | 6.2 | 6.1 | 5.7 | 5.7 | 9.8 | 9.1 | 8.5 | 9.2 | 8.8 | 8.3 | 10.1 | 10.0 | 10.9 |
Std Deviation (1e-4) | 1.4 | 1.7 | 1.9 | 2.3 | 2.0 | 1.8 | 1.7 | 2.0 | 2.1 | 1.6 | 1.6 | 1.5 | 1.8 | 1.5 | 1.4 | 2.3 | 2.2 | 1.9 | 2.1 | 2.1 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.05 | -0.24 | -0.29 | -0.23 | -0.38 | -0.43 | -0.51 | -0.51 | 0.26 | 0.16 | 0.07 | 0.17 | 0.11 | 0.03 | 0.31 | 0.3 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.3 | -1.1 | -0.4 | 0.2 | -0.6 | 1.7 | -1.4 | 0 | -1.2 | -2.9 | -2.6 | -2.3 | 0.7 | 0.1 | 0.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | -1.2 | 0 | -2.2 | -1.8 | 0.2 | 0.8 | 1.2 | 3.4 | 3.2 | 1.8 |
Cytoplasm | 0 | 1.9 | 0.7 | 1.0 | 1.3 | 0.8 | 0.5 | 1.6 | 0 | 0 | 0.8 | 0.9 | 5.5 | 3.2 | 4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.9 | -0.6 | -2.0 | -4.0 | -2.4 | -3.3 | -5.3 | -3.7 | -4.3 | 0.7 | 1.7 | 1.8 | 1.3 | 4.6 | 3.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.1 | 12.5 | 9.0 | 6.7 | 5.8 | 0 |
Mitochondria | -0.1 | -0.4 | 0.7 | 0.4 | -2.5 | 0.1 | 1.4 | 1.0 | 3.1 | -2.6 | -8.3 | -4.8 | -17.2 | -18.9 | -18.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.4 | 0.7 | 1.9 | 1.9 | 1.2 | 1.2 | 1.9 | 1.2 | 2.7 | -1.1 | -2.7 | -3.8 | -0.7 | 0 | 0.9 |
Nucleolus | 1.6 | 2.8 | 4.5 | 4.7 | 5.8 | 4.8 | 3.6 | 3.6 | 3.1 | 0 | -0.9 | -1.6 | 0 | 3.0 | 2.6 |
Peroxisomes | -2.1 | -5.4 | -8.4 | -7.8 | -5.5 | -6.3 | -9.1 | -7.7 | -9.4 | -5.3 | -6.5 | -2.8 | 1.2 | 2.0 | 1.5 |
SpindlePole | 0 | -1.3 | -2.6 | -2.2 | 1.3 | 0.5 | -1.5 | -2.3 | -1.7 | -2.3 | -0.5 | -0.8 | 1.3 | 4.1 | 4.4 |
Vacuole | 0 | 1.7 | -0.2 | 0 | -0.2 | -0.3 | 0.8 | -0.6 | -0.1 | 4.6 | 6.5 | 4.6 | 6.0 | 6.8 | 6.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.314 | 11.1564 | 10.5338 | 9.3631 | 9.1178 | 10.2615 | 8.1622 | 9.9894 | 9.1427 | 8.7945 | 8.6064 | 8.9349 | 8.3992 | 9.8545 | 8.3583 | 9.323 | 7.6635 | 8.6845 |
Actin | 0.0006 | 0.0027 | 0.0028 | 0.0006 | 0.0002 | 0.0017 | 0.0302 | 0.0018 | 0.0035 | 0.0003 | 0.0013 | 0.0055 | 0.0311 | 0.0033 | 0.0058 | 0.0019 | 0.0044 | 0.0032 |
Bud | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0 | 0.0011 | 0 |
Bud Neck | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Bud Periphery | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0004 | 0.0009 | 0.0007 | 0.0006 | 0.0001 | 0.0092 | 0.0001 |
Bud Site | 0.0011 | 0.0002 | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0023 | 0.0017 | 0.0007 | 0 | 0.0006 | 0.0001 | 0.0014 | 0.0003 | 0.0004 | 0 | 0.0008 | 0.0001 |
Cell Periphery | 0.0004 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0001 |
Cytoplasm | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0022 | 0 | 0.0001 | 0 | 0.0013 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Cytoplasmic Foci | 0.0007 | 0.0041 | 0.0008 | 0.0016 | 0 | 0.0004 | 0.0118 | 0.0026 | 0.0044 | 0.0003 | 0.0007 | 0.0047 | 0.0068 | 0.0018 | 0.0037 | 0.0006 | 0.0041 | 0.0017 |
Eisosomes | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0036 | 0 | 0.0002 | 0 | 0.0039 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0017 | 0.0001 |
Endosome | 0.0019 | 0.0178 | 0.0011 | 0.0019 | 0.0002 | 0.0012 | 0.027 | 0.0029 | 0.0078 | 0.0027 | 0.0168 | 0.0065 | 0.0256 | 0.0066 | 0.0116 | 0.001 | 0.0125 | 0.0049 |
Golgi | 0.0025 | 0.0393 | 0.005 | 0.0031 | 0.0004 | 0.0045 | 0.0249 | 0.011 | 0.0099 | 0.0061 | 0.0059 | 0.0119 | 0.0366 | 0.0343 | 0.0272 | 0.0048 | 0.0129 | 0.0108 |
Lipid Particles | 0.0043 | 0.0151 | 0.0043 | 0.0026 | 0.0002 | 0.0138 | 0.0234 | 0.0181 | 0.0114 | 0.0003 | 0.0005 | 0.0084 | 0.013 | 0.0087 | 0.0145 | 0.0072 | 0.0633 | 0.0044 |
Mitochondria | 0.9545 | 0.8917 | 0.9635 | 0.9747 | 0.9979 | 0.9667 | 0.8275 | 0.9418 | 0.9468 | 0.9885 | 0.9592 | 0.9551 | 0.8481 | 0.9196 | 0.9225 | 0.9791 | 0.8252 | 0.9642 |
None | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 |
Nuclear Periphery | 0.0001 | 0.0003 | 0.0035 | 0 | 0 | 0.0001 | 0.015 | 0.0002 | 0.0005 | 0 | 0.0022 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0026 | 0.0002 |
Nucleolus | 0.0214 | 0.0056 | 0.0003 | 0 | 0 | 0.003 | 0.0003 | 0.0012 | 0.0006 | 0 | 0 | 0 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0009 | 0.0001 |
Nucleus | 0.0013 | 0.0003 | 0.0001 | 0 | 0 | 0.0005 | 0.0026 | 0.0002 | 0.0002 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 |
Peroxisomes | 0.0055 | 0.0141 | 0.007 | 0.0142 | 0.0005 | 0.0054 | 0.0129 | 0.0113 | 0.0067 | 0.0009 | 0.0003 | 0.0017 | 0.0205 | 0.0089 | 0.0087 | 0.0047 | 0.0111 | 0.0051 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0006 | 0 | 0 | 0.0007 | 0.0002 | 0 | 0.0001 | 0 | 0.0024 | 0 |
Vacuole | 0.0023 | 0.0022 | 0.0023 | 0.0002 | 0.0001 | 0.0005 | 0.0023 | 0.0029 | 0.0025 | 0.0001 | 0.0036 | 0.0005 | 0.0042 | 0.0057 | 0.001 | 0.0001 | 0.0193 | 0.0006 |
Vacuole Periphery | 0.0023 | 0.0056 | 0.0071 | 0.0004 | 0.0003 | 0.0016 | 0.0091 | 0.0033 | 0.0034 | 0.0005 | 0.002 | 0.0034 | 0.0078 | 0.0087 | 0.002 | 0.0003 | 0.0257 | 0.0041 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 34.1762 | 18.9128 | 33.466 | 35.4196 | 37.4533 | 28.4644 | 29.7873 | 29.2879 | 30.9648 | 40.9253 |
Translational Efficiency | 0.8503 | 1.5594 | 0.8139 | 0.6623 | 0.7632 | 0.8747 | 0.8865 | 0.8625 | 0.7945 | 0.6806 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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