Standard name
Human Ortholog
Description 5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0.06 0 0 0.05 0 0.06 0 0.07 0.11 0.13 0.1 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.07 0 0.14 0.19 0.23 0.19 0.3 0.27 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.98 0.95 0.86 0.9 0.8 0.8 0.72 0.72 0.72 0.69 0.67 0.91 0.85 0.82 0.84 0.84 0.85 0.93 0.93 0.9 0.84 0.76 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.09 0.1 0.13 0.11 0.16 0.14 0.18 0 0 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0.08 0 0 0 0.06 0.09 0.17 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 4 6 0 2 2 4 0 0 0 1 1 0 1 1 0 5 6 4
Bud Neck 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 6 4 12 7 21 5 9 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 5 0 1 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 0 1 8 12 15 23 24 12 19 10 20 4 40 55 82 32 17 16 0 0 0 4 3 3
Endoplasmic Reticulum 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 8 5 2 0 0 0 0 0 0
Endosome 0 0 0 0 4 0 0 0 0 0 0 0 0 3 7 2 2 1 1 1 0 0 4 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 1 1 0 2
Mitochondria 15 5 3 14 11 54 104 123 70 133 93 125 2 0 1 1 9 2 2 1 0 1 4 7
Nucleus 234 145 191 179 295 301 433 390 267 319 234 264 540 409 513 271 286 200 228 139 187 252 367 286
Nuclear Periphery 0 0 2 5 2 5 11 11 1 4 6 2 1 6 8 2 2 1 0 0 0 0 0 6
Nucleolus 4 0 0 6 6 33 56 71 42 69 48 69 0 2 6 11 9 16 3 0 3 1 7 12
Peroxisomes 1 0 0 0 2 0 1 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 1 0 2 1 0 0 0 0 1 4 7 0 1 0 0 0 0 1 2 4
Vac/Vac Membrane 1 6 2 9 13 4 15 23 17 16 10 20 13 20 19 12 26 10 7 4 11 27 82 152
Unique Cell Count 246 148 201 209 329 376 544 540 371 445 340 393 593 480 627 323 339 234 247 150 209 300 483 495
Labelled Cell Count 256 157 206 231 350 428 654 651 424 574 418 498 598 500 647 340 362 248 247 150 209 300 483 495


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 8.1 7.9 10.4 8.1 7.1 6.9 6.9 7.4 7.0 6.8 6.9 9.7 10.1 9.7 12.5 14.0 11.8 8.8 8.3 9.0
Std Deviation (1e-4) 1.2 1.7 2.7 10.6 2.8 2.7 2.3 2.5 2.6 2.5 2.5 2.3 3.3 4.3 4.2 4.5 5.9 4.7 2.7 2.0 2.2
Intensity Change (Log2) – – – 0.4 0.04 -0.14 -0.2 -0.18 -0.09 -0.17 -0.22 -0.19 0.3 0.36 0.3 0.67 0.83 0.58 0.16 0.08 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.3 1.1 0.3 -1.3 0.6 -1.2 1.0 0 1.4 3.1 3.6 2.5 0.6 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 1.3 4.9 6.1 6.9 5.9 8.2 7.6 8.5 0 0 0 0 0 0
Nucleus -3.2 -2.2 -4.8 -5.1 -6.7 -6.6 -6.7 -7.2 -7.6 -1.8 -3.6 -4.6 -3.8 -3.7 -3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 4.3 4.7 5.4 5.0 5.9 5.6 6.3 0 0 0 0 0 3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.1 2.0 0 0 2.2 2.3 1.9 0 2.5 0 2.1 1.6 1.9 3.4 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.8126 8.7752 9.0732 7.888 7.2063 7.92 5.1133 7.8795 7.8207 6.305 5.558 6.1464 4.2592 6.2634 5.9215 5.5035 4.892 4.9111
Actin 0 0 0 0 0.0001 0 0.0074 0.0001 0.0106 0.0002 0.0054 0.0003 0 0 0 0 0 0.0002
Bud 0 0 0 0 0.0001 0 0.0007 0 0.0001 0.0002 0.0002 0.0001 0 0 0 0 0 0
Bud Neck 0 0.0001 0 0 0.0007 0.0008 0.002 0.0001 0.0006 0.0003 0.0004 0.0009 0.0001 0.0001 0 0 0.0001 0.0005
Bud Periphery 0 0.0001 0 0 0.0004 0.0001 0.0021 0.0001 0.0002 0.0003 0.0005 0.0002 0 0 0 0 0 0.0001
Bud Site 0 0 0 0 0.0001 0 0.0018 0.0002 0.007 0.0006 0.0008 0.0001 0.0001 0 0 0 0 0.0001
Cell Periphery 0 0.0001 0 0 0.0001 0 0.0007 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0 0.0001 0 0 0.0001 0.0001 0.0098 0.0003 0.0008 0.0013 0.003 0.0003 0.0003 0.0001 0 0 0 0.001
Cytoplasmic Foci 0 0 0 0 0 0 0.0195 0.0001 0.0087 0.0049 0.0226 0.0005 0.001 0 0 0 0 0.0002
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.0001 0 0.0022 0.0013 0.0003 0.0008 0.0037 0.0002 0.0001 0.0001 0 0 0 0.0004
Endosome 0 0 0 0 0.0001 0.0001 0.0224 0.002 0.0101 0.0174 0.0733 0.0005 0.0004 0 0 0 0 0.0014
Golgi 0 0 0 0 0 0 0.0045 0.0003 0.0024 0.0021 0.0171 0.0004 0 0 0 0 0 0.0001
Lipid Particles 0 0 0 0 0 0 0.0107 0.0005 0.0095 0.0146 0.0318 0.0028 0.0006 0 0 0 0 0.0001
Mitochondria 0.0002 0.0003 0.0001 0.0001 0.0009 0.0001 0.0123 0.0006 0.0012 0.0028 0.0768 0.0016 0.0006 0.0001 0.0003 0.0001 0.0001 0.0002
None 0 0 0 0 0 0 0.0023 0.0001 0.0003 0.0004 0.0015 0 0.0003 0 0 0 0 0.0001
Nuclear Periphery 0.0005 0.0059 0.0011 0.0025 0.0053 0.0007 0.013 0.0042 0.0035 0.0057 0.0114 0.0023 0.0007 0.0034 0.0007 0.0012 0.0002 0.0014
Nucleolus 0.0194 0.0189 0.0136 0.0175 0.0518 0.0464 0.0256 0.0221 0.0136 0.0279 0.0427 0.0343 0.0208 0.0064 0.0139 0.0223 0.0354 0.0231
Nucleus 0.9795 0.973 0.9849 0.9682 0.9381 0.9481 0.825 0.9629 0.9172 0.8977 0.6803 0.9517 0.9729 0.9889 0.9849 0.9762 0.9638 0.97
Peroxisomes 0 0 0 0 0 0 0.009 0 0.0075 0.0004 0.0062 0.0011 0 0 0 0 0 0
Punctate Nuclear 0.0002 0.0004 0.0001 0.0116 0.0009 0.0031 0.0105 0.0033 0.0049 0.0045 0.0138 0.0005 0.0007 0 0 0.0001 0.0001 0.0005
Vacuole 0.0001 0.0005 0 0 0.0007 0.0003 0.0121 0.0011 0.0008 0.0101 0.0045 0.0012 0.0008 0.0005 0 0 0.0001 0.0004
Vacuole Periphery 0.0001 0.0005 0.0001 0 0.0005 0.0001 0.0065 0.0006 0.0004 0.0076 0.0035 0.0008 0.0005 0.0002 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.7857 35.1971 26.1177 34.9418 71.5929 72.9437 21.0488 14.9388 29.357 63.2575
Translational Efficiency 1.6869 0.8223 0.7588 1.3879 1.1832 1.5037 1.1804 0.9382 1.0303 1.1281

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description 5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rad27

Rad27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available