Standard name
Human Ortholog
Description One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.52 0.66 0.69 0.52 0.48 0.43 0.4 0.33 0.28 0.33 0.23 0.26 0.72 0.64 0.64 0.33 0.3 0.39 0.68 0.73 0.63 0.68 0.64 0.71
Bud 0.08 0.18 0.22 0.21 0.18 0.15 0.19 0.15 0.15 0.12 0.14 0.12 0.19 0.18 0.21 0.09 0.07 0.08 0.05 0.05 0 0.05 0.08 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.14 0.12 0.05
Bud Site 0 0 0.06 0.05 0.05 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.14 0.15 0.08 0.09 0 0 0.09 0 0.09 0.06 0.1 0.09 0.18 0.21 0.23 0 0.14 0.13 0 0 0 0 0 0
Cytoplasm 0.22 0.17 0.16 0.22 0.28 0.22 0.17 0.24 0.18 0.15 0.14 0.12 0.17 0.22 0.21 0.26 0.26 0.21 0.06 0 0.05 0.05 0 0.08
Endoplasmic Reticulum 0 0.11 0 0.06 0.11 0.16 0.16 0.1 0.12 0.07 0.08 0.12 0.09 0.09 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.33 0.16 0 0 0 0 0 0
Mitochondria 0 0.08 0.11 0.36 0.12 0.37 0.36 0.39 0.6 0.57 0.62 0.67 0 0 0.1 0.05 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.08 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.07 0.1 0.09 0.08 0.08 0.08 0.05 0 0 0 0 0.19 0.12 0.06 0 0 0 0 0 0.08 0 0 0
SpindlePole 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 144 184 192 116 114 124 132 122 75 112 69 84 93 78 95 72 48 64 249 263 208 74 106 124
Bud 21 51 61 47 43 44 63 54 40 39 43 38 24 22 31 20 11 13 16 18 11 5 13 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15 26 15 15 19 9
Bud Site 3 12 17 12 13 18 12 14 4 17 7 6 2 1 1 2 0 1 – – – – – –
Cell Periphery 40 42 22 19 9 9 28 16 25 21 29 30 23 25 34 9 22 22 6 3 5 1 5 7
Cytoplasm 61 47 44 48 66 65 55 87 48 50 43 40 22 27 32 55 41 35 23 10 15 5 7 13
Endoplasmic Reticulum 12 32 12 14 27 45 51 37 33 25 24 39 12 11 6 12 1 7 7 3 3 0 2 1
Endosome 27 3 2 3 8 5 8 7 1 3 4 2 3 2 0 5 5 1 12 11 17 3 3 3
Golgi 19 11 1 1 0 2 1 6 1 2 1 2 2 4 1 43 53 26 7 4 4 0 1 0
Mitochondria 12 21 30 81 28 108 119 143 162 192 184 216 4 1 15 11 7 6 12 7 15 0 1 2
Nucleus 1 0 0 1 1 1 2 1 0 2 0 3 0 0 0 34 13 20 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 2 0 1 2 0 3 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 4 0 2 1 1 0 0 3 1 2 3 3 0 0 0 7 1 3 0 0 0 0 0 0
Peroxisomes 19 20 27 19 19 24 26 20 7 14 12 7 25 14 9 3 3 5 7 2 28 0 0 1
SpindlePole 14 4 12 1 6 3 4 8 1 3 3 4 0 1 0 6 10 12 1 1 0 0 1 0
Vac/Vac Membrane 5 6 2 1 3 5 6 15 3 9 7 9 0 0 0 7 11 4 3 3 3 1 0 0
Unique Cell Count 277 279 277 222 239 290 328 369 270 336 299 322 129 121 149 215 160 164 367 362 334 110 166 176
Labelled Cell Count 382 433 424 365 338 453 509 533 402 493 429 486 210 186 224 286 226 219 367 362 334 110 166 176


Actin, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 8.7 6.3 6.2 7.4 6.9 7.1 7.1 6.5 6.4 6.4 6.4 9.5 9.9 8.7 13.6 15.8 14.4 9.0 9.4 9.7
Std Deviation (1e-4) 1.8 1.4 1.2 1.2 1.5 1.3 1.4 1.5 1.4 1.4 1.6 1.5 1.5 1.8 1.5 4.4 5.3 4.7 1.4 2.0 1.7
Intensity Change (Log2) – – – -0.02 0.24 0.15 0.17 0.19 0.04 0.02 0.03 0.03 0.6 0.67 0.48 1.11 1.33 1.2 0.52 0.58 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.9 -5.0 -6.4 -7.1 -9.1 -9.7 -8.9 -11.1 -10.6 0.6 -1.0 -1.2 -7.9 -8.0 -6.2
Bud -0.2 -1.1 -2.1 -0.9 -2.4 -2.2 -3.5 -2.4 -3.4 -0.8 -0.9 -0.3 -3.8 -4.1 -3.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -0.3 -0.3 0 -1.4 -1.4 -2.8 -0.6 -2.3 -2.7 -2.0 -2.3 -2.7 -3.0 -3.2 -2.8
Cell Periphery 0.2 -2.0 -2.5 0.3 -1.9 0.5 -0.8 0.7 0.6 3.0 3.6 4.3 -1.7 1.9 1.9
Cytoplasm 1.6 3.2 2.0 0.3 2.4 0.6 -0.3 -0.5 -1.2 0.3 1.5 1.4 2.7 2.5 1.4
Endoplasmic Reticulum 1.0 3.0 4.4 4.5 2.7 3.4 1.6 1.8 3.4 2.0 1.9 -0.1 0.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 7.6 10.0 6.6
Mitochondria 6.8 0.3 7.3 7.2 7.9 12.0 11.9 12.6 14.0 -2.6 -3.4 -0.2 -2.3 -2.3 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 6.9 0 5.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.5 -0.7 -0.6 -0.8 -2.1 -3.5 -2.8 -2.7 -4.0 2.7 0.6 -1.3 -3.8 -3.1 -2.6
SpindlePole -2.7 -1.1 -2.4 -2.4 -1.6 -3.0 -2.7 -2.5 -2.3 0 0 0 -0.9 0.9 1.3
Vacuole 0 0 0 0 2.6 0 0 0 1.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5066 8.7209 7.6246 8.123 7.035 8.6758 6.2678 7.5576 6.372 6.4651 6.4658 7.1184 7.3364 7.7952 6.8952 7.7527 6.427 7.6004
Actin 0.8729 0.6785 0.8797 0.9858 0.8864 0.9215 0.743 0.6239 0.6811 0.8986 0.9005 0.824 0.914 0.6326 0.7961 0.8345 0.8272 0.8077
Bud 0.0007 0.0031 0.0026 0.0011 0.0034 0.0005 0.0009 0.0035 0.014 0.006 0.0005 0.0008 0.0007 0.0012 0.0146 0.0246 0.0055 0.0021
Bud Neck 0.0297 0.017 0.0284 0.0024 0.0892 0.0462 0.0166 0.0132 0.031 0.0097 0.0067 0.1151 0.0066 0.0105 0.0154 0.018 0.0315 0.128
Bud Periphery 0.0009 0.0029 0.0058 0.0015 0.0062 0.0007 0.0012 0.004 0.0375 0.0192 0.0007 0.0015 0.0016 0.0011 0.0455 0.0734 0.0439 0.0127
Bud Site 0.0386 0.2188 0.0567 0.005 0.003 0.0009 0.0352 0.2461 0.0727 0.0047 0.0015 0.0026 0.0195 0.1252 0.0421 0.008 0.0043 0.0034
Cell Periphery 0.002 0.0037 0.0011 0.0002 0.0005 0.0003 0.0013 0.0041 0.003 0.0008 0.0001 0.0005 0.0033 0.0022 0.0024 0.0018 0.0079 0.0078
Cytoplasm 0.0029 0.0504 0.0015 0.0002 0.0003 0.0005 0.022 0.0459 0.0153 0.002 0.0009 0.0008 0.0052 0.1482 0.042 0.0212 0.0082 0.0264
Cytoplasmic Foci 0.022 0.0061 0.0094 0.001 0.0013 0.009 0.0298 0.0124 0.0238 0.0058 0.0094 0.0083 0.0051 0.0422 0.0068 0.0068 0.0008 0.0034
Eisosomes 0.0047 0.001 0.0006 0.0003 0.0007 0.0066 0.0024 0.0014 0.0008 0.0009 0.0005 0.0009 0.0224 0.0007 0.0013 0.0006 0.0016 0.0008
Endoplasmic Reticulum 0.0001 0.0064 0 0 0.0001 0 0.0108 0.0004 0.003 0.0005 0.0013 0.0002 0.0018 0.0016 0.0018 0.0005 0.0003 0.0003
Endosome 0.0006 0.0025 0.0003 0.0001 0.0007 0.0009 0.0224 0.0015 0.0212 0.0221 0.0248 0.0036 0.0029 0.0035 0.0058 0.0012 0.0007 0.0003
Golgi 0.004 0.0031 0.002 0.0011 0.0052 0.0065 0.0114 0.0048 0.0173 0.0086 0.0204 0.0051 0.0065 0.0073 0.0039 0.0021 0.0022 0.0023
Lipid Particles 0.001 0.001 0.0003 0.0001 0.0009 0.0011 0.0125 0.0013 0.0133 0.0017 0.0091 0.0036 0.002 0.0014 0.0035 0.0002 0.0006 0.0005
Mitochondria 0.0004 0.0003 0.0002 0.0001 0.0008 0.0004 0.0137 0.0012 0.0079 0.0128 0.0043 0.0065 0.0007 0.0002 0.0053 0.0006 0.0632 0.0002
None 0.0001 0.0006 0 0 0 0 0.0209 0.0181 0.0088 0.0005 0.0012 0.0002 0.004 0.0138 0.0008 0.0002 0.0001 0.0003
Nuclear Periphery 0 0.0002 0 0 0 0 0.0087 0.0001 0.0061 0.0003 0.0022 0.0003 0.0008 0.0002 0.0007 0.0001 0.0001 0
Nucleolus 0 0.0001 0 0 0 0 0.0004 0 0.0004 0.0001 0 0.0016 0 0 0.0005 0 0 0
Nucleus 0 0.0002 0 0 0 0 0.0051 0.0001 0.0054 0.0002 0.0002 0.0146 0.0001 0.0002 0.0007 0.0002 0 0
Peroxisomes 0.0193 0.0035 0.0114 0.001 0.001 0.0046 0.0346 0.0172 0.0301 0.003 0.0129 0.0084 0.0021 0.0071 0.0029 0.0045 0.0015 0.0035
Punctate Nuclear 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0045 0.0003 0.0044 0.0001 0.0021 0.0008 0.0004 0.0004 0.0003 0.0001 0 0.0001
Vacuole 0 0.0006 0 0 0.0001 0 0.0017 0.0003 0.0018 0.0016 0.0002 0.0004 0.0001 0.0002 0.0062 0.0011 0.0001 0.0001
Vacuole Periphery 0 0 0 0 0 0 0.0011 0 0.001 0.0008 0.0004 0.0001 0.0001 0 0.0014 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6902 9.8147 22.6154 34.6271 13.2984 15.8821 17.1052 33.0482 33.0757 22.3959
Translational Efficiency 1.0123 0.7968 0.7983 0.6326 0.8859 0.7737 0.8278 0.5275 0.714 0.5952

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication
Localization
Cell Percentages actin (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Myo3

Myo3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Myo3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available