Standard name | |
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Human Ortholog | |
Description | Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; suggested stimulatory role in 80S formation during translation initiation; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 1 | 2 | 1 | 2 | 2 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 2 | 1 | 3 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 2 | 8 | 2 | 12 | 14 | 8 | 4 | 7 | 7 | 3 | 1 | 1 | 1 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 10 | 6 | 6 | 9 | 10 | 22 | 23 | 9 | 14 | 10 | 8 | 33 | 65 | 45 | – | – | – | 1 | 2 | 0 | 2 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 1 | 0 | 0 | 2 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | – | – | – | 0 | 4 | 0 | 1 | 5 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 2 | 0 | 2 |
Mitochondria | 11 | 47 | 109 | 76 | 98 | 187 | 349 | 248 | 208 | 280 | 120 | 118 | 22 | 23 | 9 | – | – | – | 0 | 1 | 2 | 4 | 5 | 6 |
Nucleus | 178 | 312 | 216 | 161 | 219 | 216 | 255 | 264 | 203 | 256 | 116 | 106 | 262 | 383 | 271 | – | – | – | 188 | 315 | 204 | 241 | 341 | 264 |
Nuclear Periphery | 3 | 0 | 1 | 6 | 1 | 3 | 5 | 1 | 2 | 8 | 3 | 2 | 3 | 2 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 5 |
Nucleolus | 39 | 2 | 1 | 6 | 14 | 8 | 17 | 15 | 18 | 19 | 15 | 12 | 4 | 21 | 16 | – | – | – | 3 | 1 | 4 | 14 | 43 | 46 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 2 | 0 | 2 | 1 | 0 | 0 | 5 | 3 | – | – | – | 0 | 2 | 0 | 3 | 7 | 7 |
Vac/Vac Membrane | 2 | 7 | 4 | 1 | 2 | 2 | 4 | 3 | 0 | 3 | 0 | 1 | 2 | 7 | 6 | – | – | – | 13 | 15 | 18 | 19 | 40 | 65 |
Unique Cell Count | 193 | 343 | 240 | 183 | 257 | 271 | 438 | 370 | 288 | 369 | 170 | 164 | 301 | 475 | 340 | 216 | 352 | 237 | 297 | 457 | 428 | |||
Labelled Cell Count | 238 | 379 | 342 | 267 | 348 | 443 | 670 | 568 | 445 | 592 | 273 | 250 | 328 | 510 | 359 | 216 | 352 | 237 | 297 | 457 | 428 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 5.3 | 4.3 | 4.1 | 4.1 | 3.7 | 2.9 | 3.5 | 3.5 | 3.2 | 3.5 | 3.3 | 6.0 | 5.8 | 5.8 | – | – | – | 5.5 | 5.6 | 6.6 |
Std Deviation (1e-4) | 13.4 | 1.0 | 0.7 | 0.7 | 0.9 | 1.2 | 0.7 | 0.8 | 1.0 | 0.7 | 1.3 | 1.2 | 1.4 | 1.5 | 1.6 | – | – | – | 1.2 | 1.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.07 | -0.22 | -0.55 | -0.31 | -0.28 | -0.43 | -0.31 | -0.37 | 0.49 | 0.43 | 0.44 | – | – | – | 0.37 | 0.39 | 0.62 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0.5 | 0.7 | 0.8 | 1.6 | 2.1 | 0.4 | 0.9 | 1.7 | 1.3 | 3.8 | 4.7 | 4.5 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -10.3 | -13.2 | -12.6 | – | – | – |
Nucleus | -0.7 | -1.6 | -3.2 | -8.6 | -5.5 | -5.5 | -6.0 | -5.5 | -6.2 | -1.1 | -3.2 | -3.3 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 3.0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0117 | 0 | 0.0085 | 0 | 0 | 0 | 0.0087 | 0.0008 | 0.0246 | 0 | 0.0444 | 0.0016 | 0.0007 | 0.0157 | 0.0045 | 0 | 0.0049 | 0 |
Bud | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0003 | 0.0019 | 0.0001 | 0 | 0.0004 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0022 | 0.0029 | 0.0036 | 0.0019 | 0.0001 | 0 | 0.0003 | 0.0008 |
Bud Periphery | 0.0022 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0021 | 0.0001 | 0.001 | 0 | 0.0006 | 0.0006 | 0.0015 | 0.0028 | 0.0001 | 0 | 0.0012 | 0 |
Bud Site | 0.0011 | 0.0001 | 0.001 | 0 | 0 | 0.0001 | 0.0057 | 0.0009 | 0.001 | 0 | 0.0022 | 0.0004 | 0.0003 | 0.0236 | 0.0001 | 0 | 0.0004 | 0 |
Cell Periphery | 0.0015 | 0 | 0.0003 | 0 | 0 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0.0004 | 0 |
Cytoplasm | 0.0028 | 0.0001 | 0.0263 | 0 | 0 | 0 | 0.0411 | 0.074 | 0.0009 | 0.0001 | 0.0063 | 0.0071 | 0.0018 | 0.0016 | 0.0002 | 0 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0144 | 0 | 0.0037 | 0 | 0 | 0 | 0.0304 | 0.0347 | 0.0095 | 0 | 0.0436 | 0.0107 | 0 | 0.004 | 0.011 | 0 | 0.0022 | 0 |
Eisosomes | 0.0046 | 0 | 0.001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0065 | 0 | 0.0013 | 0 | 0 | 0 | 0.0125 | 0.0041 | 0.0004 | 0 | 0.001 | 0.0006 | 0.0033 | 0.0025 | 0.0001 | 0 | 0.0015 | 0 |
Endosome | 0.0641 | 0 | 0.0039 | 0 | 0 | 0 | 0.1129 | 0.0525 | 0.0049 | 0 | 0.0079 | 0.0061 | 0.0012 | 0.0083 | 0.0127 | 0 | 0.079 | 0 |
Golgi | 0.0215 | 0 | 0.0012 | 0 | 0 | 0 | 0.0129 | 0.0236 | 0.0025 | 0 | 0.0075 | 0.0008 | 0 | 0.005 | 0.0489 | 0 | 0.0813 | 0 |
Lipid Particles | 0.4748 | 0 | 0.0042 | 0 | 0 | 0 | 0.0779 | 0.0328 | 0.0042 | 0 | 0.009 | 0.0012 | 0 | 0.0009 | 0.0053 | 0 | 0.0113 | 0 |
Mitochondria | 0.1985 | 0.0001 | 0.01 | 0.0001 | 0.0002 | 0.0001 | 0.0099 | 0.0025 | 0.0173 | 0.0001 | 0.0017 | 0.0034 | 0.0021 | 0.0207 | 0.0042 | 0.0001 | 0.7758 | 0.0001 |
None | 0.0499 | 0 | 0.0705 | 0 | 0 | 0 | 0.0987 | 0.0045 | 0.0047 | 0 | 0.0062 | 0.0079 | 0.0002 | 0.0005 | 0 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0606 | 0.0002 | 0.0018 | 0.0004 | 0.0004 | 0.0002 | 0.0415 | 0.0043 | 0.0028 | 0.0005 | 0.0016 | 0.0189 | 0.054 | 0.0044 | 0.001 | 0.0004 | 0.0017 | 0.0001 |
Nucleolus | 0.0048 | 0.0122 | 0.0092 | 0.0032 | 0.0185 | 0.0352 | 0.005 | 0.0055 | 0.0177 | 0.0089 | 0.1991 | 0.0294 | 0.0133 | 0.0136 | 0.0138 | 0.0118 | 0.0002 | 0.0338 |
Nucleus | 0.0032 | 0.9868 | 0.849 | 0.9961 | 0.9804 | 0.9632 | 0.5087 | 0.7451 | 0.8658 | 0.99 | 0.6457 | 0.8669 | 0.901 | 0.8793 | 0.8937 | 0.9871 | 0.0005 | 0.9645 |
Peroxisomes | 0.0294 | 0 | 0.0033 | 0 | 0 | 0 | 0.0054 | 0.0082 | 0.0146 | 0 | 0.0123 | 0.0045 | 0 | 0.0033 | 0.0036 | 0 | 0.0008 | 0 |
Punctate Nuclear | 0.0059 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0004 | 0.0064 | 0.0034 | 0.0226 | 0.0003 | 0.0069 | 0.033 | 0.0006 | 0.0054 | 0.0003 | 0.0005 | 0.0001 | 0.0005 |
Vacuole | 0.0174 | 0.0001 | 0.0015 | 0 | 0.0001 | 0.0001 | 0.0112 | 0.0021 | 0.0024 | 0 | 0.0006 | 0.0016 | 0.0109 | 0.0021 | 0.0001 | 0 | 0.0103 | 0 |
Vacuole Periphery | 0.0243 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0066 | 0.0006 | 0.0023 | 0 | 0.0003 | 0.002 | 0.0045 | 0.0021 | 0.0002 | 0 | 0.0269 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0815 | 13.0522 | 8.3017 | 14.8665 | 17.1277 | 6.9489 | 11.626 | 12.5124 | 11.8451 | 15.0445 |
Translational Efficiency | 1.3455 | 0.9876 | 1.4921 | 0.8023 | 0.8427 | 2.0429 | 1.2287 | 0.9756 | 1.125 | 0.9971 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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