Standard name | |
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Human Ortholog | |
Description | Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 8 | 6 | 5 | 8 | 8 |
Bud | 4 | 3 | 12 | 13 | 6 | 17 | 18 | 21 | 13 | 14 | 4 | 3 | 6 | 2 | 0 | 0 | 0 | 1 | 4 | 11 | 4 | 2 | 5 | 8 |
Bud Neck | 2 | 2 | 13 | 2 | 1 | 2 | 5 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 2 | 1 | 2 | 1 | 0 | 3 | 5 |
Bud Site | 0 | 1 | 1 | 1 | 0 | 5 | 11 | 9 | 7 | 8 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 3 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 38 | 175 | 158 | 95 | 58 | 73 | 81 | 82 | 39 | 38 | 8 | 16 | 274 | 176 | 139 | 26 | 20 | 15 | 73 | 88 | 61 | 101 | 130 | 125 |
Endoplasmic Reticulum | 0 | 2 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 5 | 13 | 0 | 3 | 0 | 2 | 1 |
Endosome | 22 | 148 | 18 | 20 | 21 | 20 | 16 | 30 | 9 | 5 | 3 | 2 | 208 | 211 | 283 | 150 | 116 | 58 | 64 | 167 | 92 | 55 | 106 | 73 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 1 | 1 | 1 |
Mitochondria | 15 | 44 | 53 | 31 | 60 | 225 | 264 | 318 | 217 | 244 | 82 | 130 | 31 | 28 | 19 | 46 | 33 | 15 | 27 | 42 | 42 | 21 | 49 | 40 |
Nucleus | 6 | 0 | 0 | 0 | 1 | 2 | 6 | 8 | 5 | 1 | 2 | 3 | 1 | 3 | 2 | 3 | 5 | 3 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 3 | 1 | 0 | 0 | 2 | 6 | 15 | 18 | 5 | 7 | 12 | 6 | 3 | 4 | 0 | 6 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 5 | 8 | 3 | 4 | 38 | 37 | 64 | 50 | 57 | 23 | 48 | 25 | 29 | 37 | 87 | 94 | 75 | 0 | 2 | 1 | 0 | 1 | 1 |
Peroxisomes | 14 | 69 | 13 | 26 | 26 | 39 | 37 | 55 | 13 | 14 | 6 | 4 | 110 | 136 | 180 | 102 | 92 | 62 | 6 | 21 | 11 | 8 | 26 | 10 |
SpindlePole | 42 | 97 | 89 | 60 | 55 | 100 | 71 | 83 | 31 | 37 | 16 | 11 | 149 | 107 | 134 | 197 | 189 | 108 | 34 | 101 | 82 | 48 | 80 | 71 |
Vac/Vac Membrane | 1 | 16 | 0 | 0 | 3 | 3 | 6 | 1 | 6 | 5 | 3 | 3 | 8 | 15 | 15 | 11 | 16 | 9 | 8 | 8 | 5 | 3 | 4 | 21 |
Unique Cell Count | 116 | 415 | 309 | 205 | 190 | 390 | 402 | 523 | 304 | 318 | 109 | 162 | 610 | 465 | 508 | 333 | 340 | 219 | 253 | 476 | 322 | 262 | 430 | 385 |
Labelled Cell Count | 150 | 569 | 367 | 252 | 238 | 527 | 559 | 686 | 409 | 429 | 155 | 237 | 820 | 712 | 814 | 628 | 576 | 357 | 253 | 476 | 322 | 262 | 430 | 385 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 4.6 | 3.5 | 3.3 | 3.6 | 3.5 | 3.3 | 3.8 | 4.0 | 4.0 | 4.5 | 4.5 | 4.7 | 4.8 | 5.3 | 7.7 | 9.6 | 8.8 | 3.7 | 3.7 | 4.6 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.2 | 0.7 | 1.2 | 1.2 | 1.2 | 1.3 | 1.6 | 1.5 | 1.5 | 1.6 | 1.3 | 1.5 | 2.1 | 3.0 | 3.3 | 3.0 | 1.1 | 1.0 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.03 | -0.03 | -0.12 | 0.11 | 0.17 | 0.17 | 0.33 | 0.34 | 0.4 | 0.45 | 0.58 | 1.13 | 1.43 | 1.32 | 0.05 | 0.08 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.2 | 3.9 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4779 | 3.8718 | 3.4236 | 3.7128 | 3.4433 | 3.7953 | 2.4962 | 2.4626 | 2.3782 | 1.4923 | 2.2872 | 1.9853 | 3.6108 | 2.7692 | 2.9841 | 3.4348 | 4.5131 | 2.9904 |
Actin | 0.0068 | 0.0157 | 0.0126 | 0.002 | 0.0011 | 0.0005 | 0.0341 | 0.034 | 0.0005 | 0.0687 | 0.0012 | 0.0003 | 0.0196 | 0.0003 | 0.0143 | 0.0038 | 0.015 | 0.0032 |
Bud | 0.0036 | 0.0018 | 0.001 | 0.0032 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0463 | 0.0001 | 0 | 0.0002 | 0.0015 | 0.0007 | 0.0009 | 0.0002 | 0.0001 |
Bud Neck | 0.0018 | 0.003 | 0.0009 | 0.0015 | 0.0016 | 0.0028 | 0.0007 | 0.0034 | 0.0007 | 0.0002 | 0.0003 | 0.0006 | 0.0004 | 0.0014 | 0.0027 | 0.0018 | 0.0003 | 0.0012 |
Bud Periphery | 0.0046 | 0.002 | 0.0007 | 0.0037 | 0.001 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0916 | 0 | 0 | 0.0004 | 0.0014 | 0.001 | 0.0015 | 0.0004 | 0.0002 |
Bud Site | 0.0108 | 0.0164 | 0.0141 | 0.0077 | 0.001 | 0.0004 | 0.0013 | 0.0089 | 0.0015 | 0.0028 | 0.0001 | 0.0001 | 0.0004 | 0.0102 | 0.0075 | 0.0028 | 0.0006 | 0.0004 |
Cell Periphery | 0.0005 | 0.0007 | 0.0002 | 0.0004 | 0.0007 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0029 | 0.0001 | 0.0001 |
Cytoplasm | 0.0212 | 0.019 | 0.0067 | 0.0069 | 0.0071 | 0.0364 | 0.0163 | 0.0194 | 0.0316 | 0.0072 | 0.0009 | 0.0365 | 0.024 | 0.0271 | 0.0157 | 0.0205 | 0.0081 | 0.0248 |
Cytoplasmic Foci | 0.275 | 0.239 | 0.2087 | 0.3077 | 0.2744 | 0.2057 | 0.3359 | 0.3156 | 0.1865 | 0.2881 | 0.3537 | 0.2484 | 0.1794 | 0.2707 | 0.1946 | 0.1414 | 0.246 | 0.2399 |
Eisosomes | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0021 | 0.0021 | 0.0011 | 0.0003 | 0.0013 | 0.0009 | 0.0049 | 0.0023 | 0.0021 | 0.0012 | 0.0003 | 0.0024 | 0.003 | 0.0017 | 0.0014 | 0.0025 | 0.0018 | 0.0005 |
Endosome | 0.0662 | 0.0813 | 0.0718 | 0.0425 | 0.1306 | 0.0592 | 0.1328 | 0.088 | 0.0745 | 0.1073 | 0.0837 | 0.1023 | 0.0724 | 0.1079 | 0.0683 | 0.1405 | 0.0439 | 0.0535 |
Golgi | 0.0255 | 0.0114 | 0.0233 | 0.0091 | 0.0158 | 0.0115 | 0.0706 | 0.0211 | 0.0163 | 0.026 | 0.1331 | 0.0063 | 0.0197 | 0.0172 | 0.0185 | 0.0306 | 0.0097 | 0.0109 |
Lipid Particles | 0.34 | 0.3117 | 0.287 | 0.3327 | 0.334 | 0.4666 | 0.1608 | 0.192 | 0.2871 | 0.1872 | 0.366 | 0.3865 | 0.4307 | 0.299 | 0.3528 | 0.2285 | 0.2704 | 0.4185 |
Mitochondria | 0.0126 | 0.0041 | 0.0099 | 0.0424 | 0.0371 | 0.0087 | 0.0063 | 0.0021 | 0.0107 | 0.0043 | 0.0034 | 0.0003 | 0.0179 | 0.0064 | 0.0037 | 0.0189 | 0.0483 | 0.002 |
None | 0.0541 | 0.1138 | 0.1461 | 0.1122 | 0.1097 | 0.0779 | 0.008 | 0.1699 | 0.2585 | 0.1377 | 0.0001 | 0.0968 | 0.0874 | 0.0817 | 0.1624 | 0.2896 | 0.2248 | 0.132 |
Nuclear Periphery | 0.0054 | 0.0056 | 0.002 | 0.0004 | 0.0041 | 0.0013 | 0.0273 | 0.0022 | 0.0023 | 0.0011 | 0.0001 | 0.0021 | 0.0355 | 0.0027 | 0.0018 | 0.0044 | 0.0257 | 0.0013 |
Nucleolus | 0.0153 | 0.0228 | 0.0211 | 0.0067 | 0.0039 | 0.0135 | 0.0024 | 0.0305 | 0.0104 | 0.0004 | 0.0001 | 0.0167 | 0.001 | 0.029 | 0.04 | 0.0017 | 0.0005 | 0.0073 |
Nucleus | 0.0154 | 0.0076 | 0.0035 | 0.0007 | 0.0018 | 0.0014 | 0.1067 | 0.0028 | 0.0057 | 0.0003 | 0 | 0.0011 | 0.0167 | 0.0093 | 0.0025 | 0.0015 | 0.0187 | 0.0018 |
Peroxisomes | 0.0806 | 0.0508 | 0.1108 | 0.095 | 0.0402 | 0.0587 | 0.0209 | 0.0689 | 0.0432 | 0.0176 | 0.0548 | 0.0652 | 0.0467 | 0.0516 | 0.0701 | 0.0543 | 0.0388 | 0.0476 |
Punctate Nuclear | 0.048 | 0.0543 | 0.074 | 0.0213 | 0.0138 | 0.0406 | 0.065 | 0.0321 | 0.0595 | 0.0094 | 0.0013 | 0.0299 | 0.0371 | 0.0484 | 0.0375 | 0.0063 | 0.044 | 0.0506 |
Vacuole | 0.0081 | 0.0313 | 0.0033 | 0.0027 | 0.0127 | 0.011 | 0.0028 | 0.0049 | 0.0064 | 0.0015 | 0.0004 | 0.0039 | 0.003 | 0.0292 | 0.0031 | 0.0409 | 0.0017 | 0.0034 |
Vacuole Periphery | 0.0021 | 0.0048 | 0.001 | 0.0008 | 0.0077 | 0.0021 | 0.0018 | 0.0008 | 0.0011 | 0.0004 | 0.0004 | 0.0005 | 0.0037 | 0.003 | 0.0008 | 0.0045 | 0.0011 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.1378 | 25.1535 | 16.8874 | 23.8047 | 16.938 | 18.0786 | 22.7077 | 27.3828 | 24.4271 | 26.2147 |
Translational Efficiency | 0.5951 | 0.5866 | 0.82 | 0.5023 | 0.6729 | 0.9137 | 0.6876 | 0.4833 | 0.7453 | 0.5754 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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