Standard name
Human Ortholog
Description Widely-conserved NADHX dehydratase; converts (S)-NADHX to NADH in ATP-dependent manner; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; downstream intergenic region drives antisense expression and mediates coordinated regulation of YKL151C and GPM1 phosphoglycerate mutase; protein abundance increases in response to DNA replication stress; homolog of Carkd in mammals and C-terminus of YjeF in E.coli

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.97 0.97 0.96 0.9 0.8 0.78 0.65 0.66 0.61 0.6 0.99 0.98 0.98 0.95 0.97 0.98 0.93 0.95 0.93 0.94 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.23 0.18 0.36 0.27 0.45 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.11 0.12 0.14 0.09 0.15 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 1 0 0 1 0 0 0 0 0 0 3 0 0 1 1 2
Bud 1 0 1 1 0 1 2 3 5 0 11 5 1 0 0 0 0 0 2 0 1 0 5 5
Bud Neck 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 4 0 4 6 4 3 1 3 5 0 0 3 4 3 6 0 0 0 0 0 0
Cytoplasm 283 129 153 252 376 269 284 307 147 101 167 141 225 277 330 95 76 94 275 124 158 143 265 293
Endoplasmic Reticulum 3 1 3 0 0 1 0 6 3 1 1 1 1 1 1 3 1 2 1 0 0 0 1 2
Endosome 0 0 0 0 0 0 0 1 0 0 1 2 1 0 0 0 0 0 1 0 1 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0
Mitochondria 10 0 0 10 11 41 80 72 81 42 123 72 0 1 1 1 0 1 2 0 3 1 2 2
Nucleus 0 0 0 0 2 3 7 5 8 6 4 9 0 0 0 0 0 0 1 0 0 0 2 0
Nuclear Periphery 0 0 0 1 0 0 3 1 1 2 3 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 2
Vac/Vac Membrane 2 1 1 5 12 12 24 43 27 21 25 36 3 8 3 5 2 1 1 1 0 0 2 2
Unique Cell Count 292 131 157 259 390 299 354 396 226 153 273 236 228 284 336 100 78 96 296 132 170 153 292 328
Labelled Cell Count 299 131 159 274 401 332 406 444 277 174 339 273 231 287 339 108 83 104 296 132 170 153 292 328


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.9 6.0 5.9 6.1 5.4 5.4 6.2 5.6 5.6 5.2 5.9 6.0 7.6 8.3 14.5 15.6 14.2 5.6 5.8 6.8
Std Deviation (1e-4) 0.7 1.5 1.8 2.2 1.7 1.2 1.3 1.5 2.5 1.5 1.4 1.6 1.6 1.9 2.5 2.9 3.6 2.5 1.3 1.8 1.6
Intensity Change (Log2) -0.01 0.05 -0.14 -0.14 0.06 -0.09 -0.1 -0.2 -0.01 0.02 0.36 0.48 1.28 1.39 1.26 -0.09 -0.04 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.6 -2.9 -5.1 -5.6 -7.6 -7.2 -8.3 -8.4 0.9 0.1 0.6 -1.0 0 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 6.5 5.7 8.4 7.1 10.0 7.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 4.0 4.2 4.5 3.6 4.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7453 3.9937 3.1652 2.8083 3.1766 3.3752 1.4499 2.0381 1.7655 2.0605 1.7103 1.7959 3.0006 3.7662 3.0731 3.5213 3.6628 3.603
Actin 0.0037 0.0003 0.0041 0.0012 0.001 0.0017 0.0132 0.0005 0.0026 0.0007 0.0113 0.0023 0.0359 0.0015 0.0143 0.0631 0.0992 0.0043
Bud 0.0022 0.0006 0.0036 0.0038 0.0002 0.0069 0.0013 0.0044 0.0004 0.0003 0.0005 0.0009 0.0006 0.0039 0.001 0.0036 0.0013 0.0004
Bud Neck 0.0003 0.0003 0.0097 0.0006 0.0006 0.0014 0.0053 0.0002 0.0004 0.0006 0.0011 0.0012 0.0183 0.0002 0.0039 0.0281 0.002 0.0029
Bud Periphery 0.0009 0.0002 0.0013 0.0006 0.0001 0.0027 0.0018 0.0012 0.0004 0.0002 0.0004 0.0007 0.0006 0.0022 0.0008 0.0023 0.0021 0.0002
Bud Site 0.0032 0.0044 0.005 0.0038 0.0002 0.0006 0.0104 0.01 0.0049 0.0002 0.0015 0.0003 0.004 0.0089 0.0028 0.0106 0.0019 0.0004
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0 0.0002 0.0003 0.0003 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0002 0.0002 0
Cytoplasm 0.8159 0.9213 0.8261 0.8393 0.9122 0.8664 0.6219 0.8392 0.7681 0.8092 0.7015 0.7389 0.7837 0.9257 0.8913 0.72 0.6802 0.9308
Cytoplasmic Foci 0.0122 0.0082 0.0122 0.0134 0.0113 0.0207 0.0372 0.0071 0.0125 0.0102 0.0346 0.0201 0.0243 0.0082 0.0099 0.047 0.0239 0.013
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0003 0 0.0002 0.0004 0.0003 0
Endoplasmic Reticulum 0.0071 0.0031 0.0025 0.0042 0.0047 0.0035 0.0056 0.0019 0.0031 0.0017 0.0012 0.0044 0.0042 0.0022 0.0039 0.0069 0.0104 0.0023
Endosome 0.0199 0.0079 0.0056 0.0142 0.0075 0.0114 0.0372 0.003 0.0123 0.0028 0.0661 0.0153 0.0165 0.0039 0.0132 0.0451 0.0584 0.0065
Golgi 0.003 0.0005 0.0004 0.0012 0.0007 0.0011 0.0074 0.0002 0.0022 0.0002 0.0256 0.0023 0.0074 0.0004 0.0041 0.0125 0.0156 0.0011
Lipid Particles 0.004 0.0001 0.0001 0.0005 0 0.0006 0.0085 0.0001 0.0032 0 0.0466 0.0065 0.0073 0.0001 0.0073 0.0045 0.0155 0.0016
Mitochondria 0.0019 0.0005 0.0005 0.0362 0.0004 0.0012 0.0038 0.0002 0.0015 0.0003 0.0292 0.0044 0.0019 0.0006 0.0075 0.0067 0.0105 0.0019
None 0.1085 0.0405 0.1056 0.0542 0.051 0.0558 0.2016 0.125 0.176 0.1663 0.034 0.1821 0.0663 0.0347 0.0195 0.0075 0.0069 0.0217
Nuclear Periphery 0.0041 0.0021 0.0029 0.0023 0.0017 0.0026 0.0137 0.0007 0.0024 0.0011 0.0009 0.0027 0.0028 0.0008 0.0057 0.0097 0.0372 0.0019
Nucleolus 0.0002 0.0001 0.0009 0.0006 0 0.0004 0.0008 0.0001 0.0006 0 0.0002 0.0002 0.0029 0.0001 0.0003 0.0004 0.0011 0.0001
Nucleus 0.0047 0.0062 0.0104 0.0085 0.0054 0.0092 0.0103 0.0027 0.0032 0.0038 0.003 0.0031 0.006 0.0035 0.0038 0.0085 0.0127 0.0058
Peroxisomes 0.0038 0.0001 0.0002 0.0011 0.0009 0.0043 0.0083 0 0.0026 0.0002 0.0357 0.0072 0.0097 0.001 0.0037 0.0128 0.0026 0.0011
Punctate Nuclear 0.0015 0.0013 0.0065 0.0115 0.0006 0.0069 0.0057 0.0007 0.0014 0.0006 0.0016 0.006 0.004 0.0006 0.0042 0.0052 0.0097 0.0027
Vacuole 0.0018 0.002 0.0017 0.0022 0.0013 0.0018 0.0041 0.0021 0.0014 0.0011 0.0034 0.001 0.0025 0.0012 0.0014 0.0029 0.005 0.001
Vacuole Periphery 0.0008 0.0003 0.0003 0.0007 0.0003 0.0005 0.0016 0.0002 0.0004 0.0002 0.0014 0.0004 0.0005 0.0002 0.001 0.0017 0.0034 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.6752 18.4824 17.3239 27.6179 19.0466 39.1524 24.343 29.2086 33.2655 27.7114
Translational Efficiency 2.0597 1.5772 1.7458 1.7921 1.6074 1.3183 1.6375 1.6047 1.7052 1.4557

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Widely-conserved NADHX dehydratase; converts (S)-NADHX to NADH in ATP-dependent manner; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; downstream intergenic region drives antisense expression and mediates coordinated regulation of YKL151C and GPM1 phosphoglycerate mutase; protein abundance increases in response to DNA replication stress; homolog of Carkd in mammals and C-terminus of YjeF in E.coli
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nnr2

Nnr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nnr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available