Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent RNA methyltransferase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.06 0 0 0 – 0 0 0 0 0 0.06 0.07 0.17 0.14 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.05 0 0 0 – 0 0 0 0 0 0.05 0.06 0.08 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.08 0.06 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.08 0.07 0.12 0.05 0.11 – 0 0 0 0 0 0.08 0.07 0 0.09 0.09 0 0 0.08 0.08 0 0
Golgi 0 0.12 0.33 0.16 0.27 0 0 – 0 0 0 0 0.11 0.33 0.4 0.48 0.31 0.22 0 0.06 0 0.06 0.07 0.08
Mitochondria 0.94 0.84 0.57 0.87 0.59 0.82 0.74 – 0.9 0.87 0.95 0.9 0.84 0.56 0.44 0.1 0.1 0.1 0.83 0.79 0.74 0.7 0.74 0.73
Nucleus 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0.06 0.05 – 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.16 0.1 0.05 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 – 0.05 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.23 0.08 0.06 0.13 0.09 0.09 – 0.08 0.08 0 0.05 0.05 0.08 0.12 0.13 0.18 0.33 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 3 3 5 0 0 – 1 2 0 0 3 13 4 5 3 1 2 2 0 0 4 8
Bud 4 0 0 5 2 5 9 – 7 3 9 5 1 1 1 5 6 8 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 1
Bud Site 0 0 0 0 0 2 0 – 2 3 3 2 0 0 0 0 1 0 – – – – – –
Cell Periphery 3 11 0 10 2 2 4 – 3 5 0 6 0 12 18 43 33 16 0 0 0 0 0 0
Cytoplasm 1 1 3 9 7 6 14 – 6 2 4 9 3 10 14 19 17 19 0 0 0 0 2 1
Endoplasmic Reticulum 6 1 2 2 7 2 5 – 1 8 3 7 4 18 15 3 9 8 0 1 0 0 0 1
Endosome 5 2 4 13 24 10 42 – 6 15 1 7 1 17 17 8 20 15 4 9 7 7 12 11
Golgi 0 24 17 29 55 3 3 – 3 3 0 4 8 71 100 120 73 37 7 13 3 5 20 28
Mitochondria 180 173 29 158 118 177 276 – 253 307 224 293 63 123 108 24 23 18 159 181 67 64 207 248
Nucleus 2 4 3 0 0 2 16 – 2 6 2 1 3 3 1 6 9 7 1 1 0 0 1 1
Nuclear Periphery 13 3 0 3 2 13 18 – 10 23 8 16 0 6 4 3 5 2 2 4 0 0 1 0
Nucleolus 8 4 0 0 3 9 16 – 5 4 5 3 0 0 0 3 2 6 0 0 0 0 1 1
Peroxisomes 2 5 0 5 3 1 6 – 1 3 3 6 1 1 8 16 37 18 9 4 6 4 9 9
SpindlePole 1 1 2 2 8 9 20 – 13 21 2 12 0 3 5 11 7 10 0 0 0 0 0 2
Vac/Vac Membrane 17 47 4 11 27 20 33 – 23 29 4 16 4 17 29 33 41 56 1 6 0 3 15 19
Unique Cell Count 192 205 51 181 201 216 375 281 354 237 324 75 218 248 250 232 172 191 230 91 92 281 341
Labelled Cell Count 243 277 67 250 263 261 462 336 434 268 388 91 295 324 299 286 221 191 230 91 92 281 341


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.6 7.5 7.3 7.6 6.0 6.2 – 5.1 5.4 4.6 5.2 6.6 7.5 7.1 9.6 9.6 8.8 6.0 7.4 7.9
Std Deviation (1e-4) 1.3 1.1 1.3 1.3 1.5 1.2 1.5 – 1.3 1.2 1.0 1.4 1.2 1.2 1.1 2.1 2.2 2.1 0.7 1.3 1.4
Intensity Change (Log2) – – – -0.04 0.01 -0.32 -0.28 – -0.56 -0.47 -0.72 -0.54 -0.2 -0.01 -0.1 0.35 0.34 0.23 -0.34 -0.02 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 – 0 0 0 0 3.2 2.9 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Endosome 0 0.8 0 0.7 – 0 0 – 0 0 0 0 0 0 0
Golgi -2.7 -0.8 0 0 – 0 0 – 0 -3.1 -0.1 0.9 1.9 -0.3 -1.7
Mitochondria 4.9 0.2 3.8 2.5 – 6.1 5.3 – 6.4 3.4 -0.1 -1.7 -8.1 -7.8 -7.1
Nuclear Periphery 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 – 0 0 0 0 0 3.1 0
SpindlePole 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 – 0 0 0 0 0 0 1.1
Nucleus 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Vacuole 0 1.1 0 0 – 0 0 – 0 0 0 0.8 1.1 1.7 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9044 3.0135 2.3102 3.2014 1.9291 2.7818 4.1332 4.3068 4.1316 3.7802 3.7099 4.3698 2.7792 3.7295 3.2035 2.9987 2.5472 3.2417
Actin 0.0261 0.0074 0.0073 0.0195 0.0022 0.0008 0.0052 0.0009 0.0021 0.0002 0.0185 0.0013 0.019 0.0059 0.0233 0.0012 0.0445 0.0011
Bud 0.0002 0.0003 0.0003 0.003 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0002 0 0.0004 0.011 0.0014 0.0001 0.0002 0.0001
Bud Neck 0.0008 0.0003 0.0002 0.0009 0.0001 0 0.0002 0.0001 0 0 0.0043 0 0.0003 0.0003 0.0002 0.0003 0.0008 0.0001
Bud Periphery 0.0007 0.0005 0.0008 0.0058 0.0001 0.0003 0.0002 0.0002 0.0004 0.0001 0.0003 0.0001 0.0009 0.006 0.0046 0.0003 0.0004 0.0002
Bud Site 0.0015 0.0009 0.0007 0.0266 0.0002 0.0001 0.0002 0.0003 0.0002 0 0.0014 0.0001 0.0009 0.0102 0.004 0.0001 0.0002 0.0001
Cell Periphery 0.0034 0.0002 0.0003 0.002 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0015 0.0005 0.0001 0.0001 0.0001
Cytoplasm 0.0032 0.0001 0.0003 0.0005 0 0.0003 0.0002 0.0002 0.0001 0 0.0001 0 0.0004 0.0007 0.0007 0 0.0001 0.0001
Cytoplasmic Foci 0.03 0.0068 0.0143 0.0026 0.0115 0.0019 0.0056 0.0044 0.0027 0.0002 0.002 0.0017 0.0099 0.0068 0.0048 0.0051 0.0048 0.0013
Eisosomes 0.0095 0.0002 0.0004 0.0008 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0004 0.0004 0.0005 0.0002 0.0005 0.0001
Endoplasmic Reticulum 0.0099 0.0001 0.0002 0.0002 0 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0009 0.0002 0.0009 0 0.0009 0.0003
Endosome 0.0132 0.0195 0.017 0.0038 0.0039 0.0143 0.0193 0.0146 0.0095 0.0009 0.0043 0.0083 0.0263 0.0331 0.0139 0.0064 0.0267 0.0153
Golgi 0.0096 0.0294 0.0203 0.0047 0.0103 0.0086 0.025 0.035 0.0207 0.0037 0.0166 0.0187 0.0435 0.0407 0.0246 0.0115 0.0361 0.021
Lipid Particles 0.0388 0.0264 0.0286 0.0029 0.0111 0.0023 0.0165 0.0062 0.0043 0.0003 0.0015 0.0027 0.037 0.0264 0.0055 0.0042 0.0142 0.0122
Mitochondria 0.7325 0.8311 0.8703 0.9184 0.8746 0.9566 0.8951 0.9197 0.9465 0.9931 0.9458 0.9487 0.8055 0.8319 0.8882 0.9545 0.858 0.9371
None 0.0089 0.0001 0.0009 0.0002 0 0.0001 0.0006 0.0001 0.0001 0 0.0001 0 0.0008 0.0001 0.0016 0 0.0003 0
Nuclear Periphery 0.001 0.0001 0.0002 0.0001 0 0.0001 0.001 0.0002 0.0001 0 0.0001 0.0001 0.0012 0.0002 0.0009 0 0.0031 0.0006
Nucleolus 0.0008 0.0003 0.0004 0.0005 0 0 0.0003 0 0.0001 0 0.0001 0.0001 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0005 0.0001 0.0002 0.0004 0 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0005 0.0002 0 0.0002 0.0002
Peroxisomes 0.0943 0.07 0.0326 0.0037 0.0847 0.0113 0.0245 0.0111 0.0099 0.001 0.003 0.0159 0.0313 0.0131 0.0167 0.0147 0.004 0.0043
Punctate Nuclear 0.005 0 0.0002 0.0001 0.0003 0 0.0009 0 0 0 0.0006 0 0.0123 0.0001 0.0002 0 0.0007 0
Vacuole 0.0069 0.0024 0.0021 0.0025 0.0002 0.001 0.0018 0.0017 0.001 0.0001 0.0002 0.0005 0.003 0.0066 0.0028 0.0004 0.0014 0.0028
Vacuole Periphery 0.0032 0.0037 0.0024 0.0007 0.0006 0.0017 0.0027 0.0047 0.0019 0.0003 0.0004 0.0013 0.0051 0.0038 0.0044 0.0006 0.0027 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6948 8.3635 10.4728 14.1316 12.5093 12.2727 14.2701 16.5874 17.2238 16.5076
Translational Efficiency 0.8484 1.1536 0.7391 0.7644 0.6774 0.8625 0.8507 0.6904 0.6831 0.7019

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent RNA methyltransferase
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rsm22

Rsm22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsm22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available