Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.07 0.07 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.98 0.94 0.86 0.74 – 0.71 0.66 0.7 0.64 0.97 0.98 0.97 0.99 1.0 0.99 0.94 0.95 0.95 0.91 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.15 – 0.2 0.23 0.16 0.22 0 0 0 0.06 0.05 0 0 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 8 12 – 16 21 26 35 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 3 0 – 2 2 3 1 0 0 0 0 0 0 0 0 0 1 1 8
Bud Neck 0 0 0 0 0 2 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 9 6 12 14 20 – 29 32 35 36 2 7 5 2 4 2 0 0 1 1 0 0
Cytoplasm 233 163 337 259 294 287 356 – 305 293 255 308 280 447 359 280 205 137 214 149 321 273 305 424
Endoplasmic Reticulum 2 1 8 6 13 24 74 – 84 103 58 107 7 15 11 17 11 4 3 0 5 14 12 15
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 4 20 – 14 27 25 29 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 1 4 0 – 9 3 6 18 1 0 1 1 0 1 1 0 1 0 0 1
Nucleus 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 4 12 – 2 4 7 4 1 0 0 0 2 0 0 2 0 3 3 4
Unique Cell Count 236 164 343 265 313 333 479 427 441 366 480 288 458 371 282 206 138 227 157 337 301 331 460
Labelled Cell Count 237 167 354 272 324 350 495 463 485 415 538 291 469 376 300 222 144 227 157 337 301 331 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 116.1 112.5 92.9 80.5 65.1 40.4 44.7 – 36.7 36.4 33.3 32.1 106.5 100.1 108.1 150.3 138.7 155.8 102.1 107.9 113.9
Std Deviation (1e-4) 26.8 25.7 16.8 16.3 13.4 12.7 9.4 – 8.6 8.1 8.3 7.6 25.0 24.0 23.7 38.9 37.5 40.8 28.0 29.4 27.0
Intensity Change (Log2) – – – -0.21 -0.51 -1.2 -1.06 – -1.34 -1.35 -1.48 -1.53 0.2 0.11 0.22 0.69 0.58 0.75 0.14 0.22 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.0 – 3.6 4.1 5.0 5.1 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 0.9 1.1 1.2 – 2.7 2.9 3.8 3.0 -1.8 -1.1 -1.2 0 0 0
Cytoplasm -0.5 -2.9 -5.9 -9.3 – -9.9 -11.1 -10.2 -11.7 -0.9 -0.6 -1.3 1.2 1.3 0.8
Endoplasmic Reticulum -0.1 1.3 3.0 6.2 – 7.4 8.4 6.2 8.1 0.1 0.8 0.5 2.3 1.9 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.8 – 3.4 4.7 4.9 4.6 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 79.4699 112.0922 103.6246 94.9997 86.1518 92.037 100.4563 133.4568 117.513 103.6188 89.6878 107.0266 114.759 136.7962 130.4045 120.7661 110.8712 108.4415
Actin 0.0168 0.0006 0.0019 0.0121 0.0024 0.0209 0.0119 0.0005 0.0028 0.0033 0.0067 0.0646 0.0019 0.0011 0.0051 0.0003 0.0051 0.002
Bud 0.0049 0.0007 0.0013 0.004 0.0015 0.0039 0.0031 0.0034 0.003 0.0235 0.0027 0.0019 0.0035 0.001 0.0042 0.002 0.0027 0.0009
Bud Neck 0.001 0.0006 0.0013 0.0021 0.001 0.0048 0.0008 0.0004 0.0008 0.0017 0.0006 0.0017 0.0008 0.0006 0.0008 0.0008 0.0013 0.0021
Bud Periphery 0.0069 0.0005 0.0012 0.0025 0.0017 0.0069 0.0035 0.0008 0.0022 0.0185 0.0038 0.0009 0.0053 0.0005 0.0028 0.0008 0.0024 0.0002
Bud Site 0.0013 0.0008 0.0014 0.0012 0.0004 0.0014 0.0039 0.0036 0.0014 0.0028 0.0021 0.001 0.0011 0.0006 0.0033 0.0002 0.0005 0.0007
Cell Periphery 0.039 0.0222 0.0182 0.026 0.0051 0.0068 0.023 0.0035 0.0105 0.0209 0.0036 0.0025 0.021 0.0054 0.0072 0.0017 0.0063 0.0004
Cytoplasm 0.5734 0.8891 0.8059 0.678 0.7579 0.6435 0.6397 0.9544 0.8372 0.6073 0.6269 0.5977 0.6475 0.9273 0.802 0.737 0.7159 0.7807
Cytoplasmic Foci 0.0167 0.0011 0.0046 0.0081 0.0073 0.0124 0.023 0.0017 0.0049 0.0295 0.0164 0.0462 0.0064 0.0013 0.0021 0.0038 0.0047 0.0266
Eisosomes 0.0004 0 0.0001 0.0004 0.0001 0.0002 0.0011 0 0.0002 0.0003 0.0001 0.0002 0.0003 0.0001 0 0.0003 0 0.0001
Endoplasmic Reticulum 0.0447 0.0136 0.0262 0.0109 0.0073 0.0128 0.0278 0.0039 0.0081 0.0153 0.0017 0.1171 0.0523 0.0036 0.0154 0.0033 0.0067 0.0487
Endosome 0.0157 0.0023 0.0135 0.0269 0.0224 0.053 0.0135 0.0026 0.0078 0.01 0.0983 0.028 0.0111 0.0027 0.0072 0.0109 0.0172 0.0179
Golgi 0.0034 0.0013 0.0028 0.0075 0.0017 0.0087 0.0033 0.0005 0.002 0.0012 0.0164 0.0201 0.0015 0.0004 0.0013 0.0003 0.005 0.0029
Lipid Particles 0.0075 0.0012 0.0029 0.01 0.0023 0.0043 0.0096 0.0003 0.0036 0.0175 0.0015 0.0033 0.0031 0.0005 0.0006 0.0014 0.0033 0.0039
Mitochondria 0.0099 0.0005 0.0041 0.0046 0.016 0.0055 0.0212 0.0004 0.0118 0.0358 0.0593 0.0138 0.0089 0.0009 0.0046 0.0018 0.0049 0.003
None 0.0058 0.0002 0.0014 0.0011 0.0026 0.0021 0.0155 0.0009 0.0028 0.0042 0.0005 0.0331 0.0054 0.0003 0.0006 0.0096 0.0013 0.0171
Nuclear Periphery 0.0281 0.0039 0.0109 0.0234 0.0085 0.0342 0.0281 0.0017 0.0136 0.0089 0.004 0.0044 0.0351 0.0106 0.0117 0.011 0.0109 0.0089
Nucleolus 0.0084 0.0005 0.0026 0.0007 0.0075 0.0019 0.0048 0.0003 0.0018 0.0064 0.0004 0.0021 0.0037 0.0001 0.0005 0.0012 0.0011 0.0035
Nucleus 0.0196 0.0023 0.0051 0.0072 0.008 0.0135 0.0116 0.0016 0.0048 0.0072 0.0035 0.0045 0.0129 0.0036 0.0047 0.0058 0.0064 0.0062
Peroxisomes 0.0055 0 0.0004 0.0058 0.0004 0.0005 0.0029 0 0.0003 0.0042 0.001 0.0009 0.0003 0 0.0002 0.0002 0.0003 0.0003
Punctate Nuclear 0.0017 0 0.0003 0.004 0.0002 0.007 0.0042 0.0001 0.0007 0.0003 0.0006 0.0219 0.0014 0.0001 0.0001 0.0003 0.0002 0.0597
Vacuole 0.1485 0.0537 0.0767 0.1296 0.0939 0.1018 0.1148 0.0158 0.0686 0.1396 0.1187 0.0242 0.1285 0.0346 0.0969 0.147 0.1272 0.0081
Vacuole Periphery 0.0407 0.0049 0.0172 0.0339 0.0516 0.0541 0.0327 0.0036 0.0111 0.0418 0.0312 0.0099 0.0479 0.0047 0.0286 0.0605 0.0766 0.0058

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 558.3492 443.6712 473.2583 628.8259 548.6886 469.4623 507.6739 473.0808 442.3831 565.6571
Translational Efficiency 1.7952 2.7526 2.6602 1.2313 2.3588 2.4892 2.1505 2.4696 2.6621 2.4046

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps27a

Rps27a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps27a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available