Standard name
Human Ortholog
Description Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 – 0 0 0 0 0 0.06 0.09 0.11 0.06 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0 0.05 – 0.06 0.08 0.09 0.12 0.12 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.93 0.8 0.79 0.83 0.72 0.73 – 0.59 0.6 0.58 0.58 0.57 0.78 0.74 0.72 0.77 0.76 0.73 0.85 0.7 0.67 0.56 0.57 0.54
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.51 0.45 0.54 0.37 0.43 0.53 – 0.62 0.71 0.66 0.65 0.61 0.47 0.4 0.36 0.22 0.35 0.31 0.12 0.24 0.27 0.35 0.35 0.36
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Bud 0 0 2 0 0 1 – 1 0 0 1 5 0 0 0 0 0 0 0 0 4 2 7 6
Bud Neck 0 0 1 0 1 0 – 4 2 4 3 9 0 0 0 0 1 0 0 0 0 1 0 1
Bud Site 0 0 0 1 7 7 – 11 10 10 20 36 0 5 2 0 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 8 19 33 17 – 29 11 17 13 12 28 65 77 20 7 14 0 0 1 1 0 4
Endoplasmic Reticulum 0 0 0 1 0 0 – 0 0 0 0 0 1 0 0 1 2 1 0 0 1 0 1 0
Endosome 0 0 1 0 1 1 – 0 0 0 0 0 0 5 5 1 2 2 0 1 8 2 4 2
Golgi 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 1 0 0 0 0 2 2 1 3
Mitochondria 0 6 41 15 20 37 – 62 70 88 82 109 8 3 0 7 12 8 0 0 2 0 1 3
Nucleus 96 113 496 340 435 543 – 627 501 591 407 496 356 532 491 240 136 175 88 98 439 191 310 339
Nuclear Periphery 0 0 4 0 4 2 – 8 5 10 9 8 1 0 0 4 0 3 0 0 2 0 0 0
Nucleolus 53 63 341 153 259 394 – 654 589 670 458 536 215 285 247 69 62 73 12 33 174 118 190 228
Peroxisomes 0 1 1 0 0 0 – 0 0 0 0 3 1 4 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 0 0 0 – 2 1 3 0 0 5 19 13 2 0 2 0 1 4 0 3 4
Vac/Vac Membrane 0 2 2 0 4 0 – 4 0 6 0 3 2 5 5 4 2 7 1 2 9 13 18 30
Unique Cell Count 103 141 631 410 604 748 1058 831 1018 705 875 459 721 683 310 179 239 104 142 657 343 541 635
Labelled Cell Count 149 191 899 529 764 1002 1402 1189 1399 993 1218 619 925 847 349 224 287 104 142 657 343 541 635


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 20.4 10.7 13.7 12.2 11.3 – 11.1 10.2 10.3 9.9 9.9 16.3 17.8 18.0 18.4 15.2 15.7 15.9 18.0 18.1
Std Deviation (1e-4) 4.0 6.0 3.2 4.6 4.6 3.6 – 3.9 3.5 3.8 3.5 3.7 7.2 7.1 6.9 7.5 5.9 7.1 5.0 6.2 5.9
Intensity Change (Log2) – – – 0.36 0.19 0.08 – 0.06 -0.06 -0.05 -0.1 -0.11 0.61 0.74 0.75 0.79 0.51 0.55 0.58 0.76 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 4.3 5.2 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.3 4.1 1.4 – 2.0 0.1 0.6 0.8 0.2 4.4 6.3 7.4 4.4 0 3.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.0 -2.6 -1.2 – -0.5 1.4 1.6 3.2 3.8 -3.7 -6.3 -6.8 -2.8 0.1 -1.8
Nucleus 1.7 -2.7 -2.6 – -8.1 -7.4 -8.6 -8.1 -8.9 -0.4 -2.1 -2.8 -0.4 -0.7 -1.7
Nuclear Periphery 0 0 0 – 0 0 0.7 1.2 0.6 0 0 0 0 0 0
Nucleolus -5.3 -3.9 -0.5 – 3.1 6.6 4.8 4.1 2.8 -2.3 -5.3 -6.5 -9.2 -4.6 -6.2
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 3.4 2.7 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.2722 14.7748 13.6053 12.4446 13.2221 12.9744 11.02 12.2698 11.5516 10.8577 10.0982 10.6489 12.6508 15.9843 14.6633 14.5174 12.8918 13.8153
Actin 0.0051 0.0007 0.0008 0.0015 0 0.0001 0.0071 0.0001 0.0063 0.0008 0.0013 0.0006 0.0552 0 0.0032 0.0001 0.0081 0.0002
Bud 0.0003 0 0.0003 0.0001 0.0003 0 0.0009 0 0.0002 0.0001 0.0002 0.0001 0.0018 0.0001 0.0004 0.0002 0.0001 0.0001
Bud Neck 0.0003 0 0.0002 0.0001 0.0001 0.0002 0.0018 0 0.0002 0.0001 0.0001 0.0003 0.0013 0 0.0001 0 0.0003 0.0002
Bud Periphery 0.0003 0 0.0005 0.0002 0.0007 0.0001 0.0015 0 0.0003 0 0.0005 0.0002 0.0026 0.0001 0.0007 0.0004 0.0004 0.0001
Bud Site 0.0015 0.0003 0.0035 0.0004 0.0007 0.0001 0.0085 0.0007 0.0008 0.0015 0.0008 0.0002 0.0057 0.0001 0.0005 0.0002 0.0003 0.0002
Cell Periphery 0.0003 0 0.0005 0.0004 0.0006 0.0001 0.0006 0 0.0002 0 0.0002 0.0003 0.0015 0 0.0003 0.0003 0.0001 0.0001
Cytoplasm 0.0028 0 0.0004 0.0006 0 0 0.0054 0 0.0004 0.001 0.0002 0.0002 0.008 0.0001 0.0056 0.0001 0.0007 0.0002
Cytoplasmic Foci 0.0141 0 0.002 0.0036 0.0001 0.0015 0.0141 0.0017 0.0025 0.0114 0.0037 0.0022 0.0302 0.0042 0.0013 0.0023 0.0129 0.0022
Eisosomes 0.0001 0.0001 0 0 0 0 0.0002 0 0.0002 0 0.0001 0 0.0005 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0011 0 0.0004 0.0004 0 0 0.0015 0 0.0005 0.0005 0.0003 0.0001 0.0085 0 0.0012 0 0.0016 0.0001
Endosome 0.0179 0 0.0032 0.0221 0 0.0003 0.0132 0.0001 0.0022 0.0097 0.0118 0.0008 0.022 0 0.0038 0.0001 0.0094 0.0018
Golgi 0.0059 0 0.0011 0.0087 0 0.0003 0.0042 0.0001 0.0033 0.0018 0.0027 0.0006 0.0065 0 0.0011 0.0001 0.0104 0.0004
Lipid Particles 0.0193 0.0001 0.002 0.0085 0.0002 0.0057 0.0134 0.0018 0.0041 0.0054 0.0288 0.001 0.0273 0.0005 0.0025 0.0053 0.0239 0.0019
Mitochondria 0.0091 0.0076 0.0054 0.0147 0.0135 0.0068 0.013 0.0006 0.005 0.0003 0.039 0.0069 0.0169 0.0002 0.0145 0.0196 0.0078 0.0052
None 0.0025 0 0.0002 0.0001 0.0001 0 0.0052 0 0.0005 0.0006 0.0001 0.0001 0.0127 0 0.0004 0.0003 0.004 0.0001
Nuclear Periphery 0.0059 0.0006 0.0042 0.0005 0.0001 0.0003 0.0048 0.0003 0.0018 0.0008 0.0008 0.0001 0.0285 0.0013 0.0025 0.0006 0.0288 0.0007
Nucleolus 0.1433 0.0508 0.07 0.0601 0.4482 0.2611 0.1342 0.0641 0.0543 0.0372 0.3528 0.2916 0.1138 0.0512 0.0678 0.0678 0.3868 0.2958
Nucleus 0.751 0.938 0.8995 0.8722 0.5332 0.7216 0.7408 0.9293 0.909 0.9229 0.5516 0.6907 0.6146 0.9414 0.8881 0.8986 0.4696 0.6842
Peroxisomes 0.0065 0.0002 0.0002 0.0011 0.0004 0.0003 0.005 0.0008 0.0056 0.0018 0.0007 0.0008 0.0061 0 0.0005 0.0018 0.0188 0.0012
Punctate Nuclear 0.0058 0.0013 0.0007 0.0001 0.0003 0.0003 0.0192 0.0002 0.0012 0.0037 0.0006 0.0011 0.0261 0.0002 0.0022 0.0003 0.0054 0.0026
Vacuole 0.0044 0 0.003 0.0027 0.0007 0.0004 0.0035 0.0001 0.0005 0.0002 0.0028 0.0008 0.0051 0.0002 0.0012 0.0006 0.0017 0.0008
Vacuole Periphery 0.0024 0 0.0018 0.0018 0.0007 0.0005 0.0019 0.0001 0.0008 0.0001 0.001 0.0011 0.005 0.0002 0.0019 0.0014 0.0087 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.5756 94.584 115.3046 140.8884 122.4839 118.3363 137.7582 168.2816 177.6888 124.4234
Translational Efficiency 2.86 2.4419 1.9116 1.832 1.6774 2.0377 1.746 1.6203 1.6023 1.9265

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Elf1

Elf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available