Standard name
Human Ortholog
Description O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0.08 0.06 0.05 0.06 0.09 0.09 0 0 0 0 0 0 0.11 0.11 0.05 0.07 0.07 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.18 0.19 0 0 0 0 0 0
Cytoplasm 0.77 0.88 0.87 0.93 0.96 0.9 0.9 0.84 0.84 0.8 0.78 0.77 0.87 0.94 0.96 0.94 0.93 0.93 0.41 0.57 0.6 0.72 0.72 0.62
Endoplasmic Reticulum 0.2 0.1 0.08 0 0 0 0 0 0 0 0 0 0.11 0 0 0.05 0 0 0.31 0.15 0.15 0.07 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0.08 0.12 0.1 0.15 0.11 0.13 0 0 0 0 0.05 0 0.06 0.05 0.06 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 3 0 0 2 4 3 7 9 11 10 0 1 2 2 1 1 5 8 6 9 14 26
Bud 1 1 2 2 1 9 21 19 12 18 21 18 0 1 2 4 7 4 18 31 10 17 33 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 9 4 8 13 19
Bud Site 0 0 0 0 0 0 2 2 3 2 6 2 1 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 8 3 3 6 6 4 3 4 4 6 1 3 2 60 37 43 0 1 1 0 3 3
Cytoplasm 118 237 155 122 179 207 236 260 204 235 182 161 184 383 425 299 196 213 69 159 115 191 327 262
Endoplasmic Reticulum 30 27 15 5 3 2 1 1 2 5 6 8 23 3 12 16 4 7 52 40 28 19 9 10
Endosome 0 0 0 0 0 0 1 4 3 2 1 1 0 3 1 1 0 0 1 5 4 3 12 28
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 2 0 0 0 2 3
Mitochondria 3 1 0 0 0 1 3 5 5 7 15 10 1 0 0 1 2 2 2 2 1 1 3 12
Nucleus 0 1 1 2 1 1 4 4 3 6 2 4 0 1 2 0 0 0 0 1 1 0 1 1
Nuclear Periphery 0 1 0 0 0 0 1 1 0 1 2 0 0 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 0 0 0 0 0 0 3 1 0 1 0 0 0 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 2 2 2 1 0 1 0 0 0 1 0 3 10
Vac/Vac Membrane 2 9 3 4 6 18 21 38 25 44 25 27 8 16 2 6 11 3 10 12 12 8 18 20
Unique Cell Count 153 270 178 131 187 229 262 308 243 293 233 210 212 407 443 317 210 229 170 280 191 265 457 426
Labelled Cell Count 156 280 187 138 193 246 300 345 268 333 276 249 220 414 449 391 260 277 170 280 191 265 457 426


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 13.6 9.1 8.8 9.6 7.8 6.9 7.1 6.6 6.3 6.0 6.7 13.0 10.4 9.1 23.6 24.9 27.7 10.6 9.6 8.8
Std Deviation (1e-4) 4.0 7.2 3.1 3.7 3.4 2.1 2.3 1.9 2.0 2.1 2.2 2.5 7.7 4.6 3.7 15.1 16.9 20.7 3.2 2.7 2.3
Intensity Change (Log2) – – – -0.04 0.08 -0.21 -0.39 -0.35 -0.46 -0.51 -0.59 -0.43 0.52 0.19 0.0 1.38 1.45 1.61 0.22 0.09 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 1.7 1.7 0 0 0 0 0 0
Bud 0 0 0 3.2 2.6 2.2 2.6 3.4 3.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -1.6 -1.0 -1.3 0 0 0 -1.7 -0.9 0 0 0 4.5 4.0 4.3
Cytoplasm 1.7 3.0 1.1 1.0 -0.8 -0.9 -1.9 -2.3 -2.6 -0.1 2.9 4.0 2.8 2.1 2.0
Endoplasmic Reticulum -1.6 -3.0 -3.8 -4.4 -4.8 -3.9 -3.5 -2.7 -1.9 0.8 -5.0 -3.2 -1.5 -3.0 -2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.8 2.9 4.1 3.5 4.7 3.6 4.1 1.2 1.4 0 0 1.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.2065 9.91 6.2144 4.8737 4.7575 9.7417 9.6111 8.944 7.1441 5.4848 4.2277 10.3868 13.6131 13.1957 9.5827 6.137 5.7417 12.8705
Actin 0.0188 0.0056 0.0108 0.0046 0.0055 0.0047 0.0339 0.0039 0.0132 0.0018 0.0032 0.008 0.0184 0.0054 0.0215 0.0059 0.0524 0.0024
Bud 0.0017 0.0086 0.0079 0.0222 0.0046 0.0038 0.0014 0.0015 0.0055 0.0221 0.0053 0.0019 0.0011 0.0033 0.0043 0.0072 0.023 0.0012
Bud Neck 0.0014 0.0011 0.0016 0.0017 0.0021 0.0051 0.014 0.0006 0.0016 0.0006 0.0006 0.0039 0.0011 0.0008 0.0042 0.0008 0.0027 0.0036
Bud Periphery 0.0025 0.0051 0.0086 0.0162 0.0014 0.0007 0.001 0.0007 0.0036 0.0238 0.008 0.0017 0.0022 0.0025 0.0034 0.0112 0.0203 0.0012
Bud Site 0.0028 0.0149 0.0084 0.0019 0.0023 0.0004 0.004 0.0051 0.0071 0.001 0.0011 0.0003 0.0017 0.0087 0.0031 0.0024 0.0041 0.0002
Cell Periphery 0.0004 0.0006 0.0005 0.0002 0.0002 0.0002 0.0004 0.0002 0.0004 0.0001 0.0001 0.0002 0.0005 0.0013 0.0003 0.0005 0.0005 0.0001
Cytoplasm 0.6822 0.8237 0.7574 0.797 0.7335 0.7529 0.7449 0.8724 0.7988 0.8152 0.6563 0.7376 0.7585 0.8728 0.85 0.8109 0.7058 0.8369
Cytoplasmic Foci 0.0262 0.0073 0.0198 0.0299 0.0441 0.023 0.0148 0.0068 0.0109 0.0067 0.0295 0.0077 0.0102 0.0045 0.0172 0.0099 0.0146 0.0051
Eisosomes 0.0003 0 0.0001 0 0 0 0.0002 0 0.0002 0 0 0.0001 0.0001 0 0.0002 0 0.0003 0
Endoplasmic Reticulum 0.0871 0.0375 0.0151 0.0173 0.0176 0.1142 0.0559 0.048 0.0293 0.0046 0.0028 0.1429 0.0846 0.0496 0.0117 0.0165 0.0201 0.0928
Endosome 0.0249 0.0048 0.0197 0.0194 0.0255 0.0088 0.025 0.0034 0.0095 0.0043 0.008 0.01 0.0231 0.0029 0.0212 0.0096 0.0139 0.0066
Golgi 0.005 0.0006 0.0018 0.001 0.0064 0.0008 0.0059 0.0004 0.0028 0.0002 0.002 0.0012 0.0053 0.0004 0.004 0.0005 0.0122 0.0007
Lipid Particles 0.008 0.0012 0.0021 0.0008 0.0015 0.0006 0.0061 0.0003 0.0051 0 0.0073 0.0042 0.0069 0.0012 0.0033 0.0002 0.0115 0.0001
Mitochondria 0.0064 0.0009 0.0014 0.0012 0.0022 0.0006 0.0021 0.0003 0.0019 0.0012 0.0017 0.005 0.0116 0.0018 0.0094 0.0013 0.0042 0.0008
None 0.032 0.0197 0.0779 0.0317 0.0906 0.0102 0.0115 0.0337 0.0682 0.1016 0.1927 0.007 0.0065 0.0141 0.0049 0.0781 0.0569 0.0027
Nuclear Periphery 0.0342 0.0243 0.0158 0.0137 0.0204 0.032 0.0295 0.0085 0.0142 0.0029 0.0025 0.027 0.0319 0.0095 0.0111 0.0123 0.0086 0.019
Nucleolus 0.0022 0.0004 0.0006 0.0003 0.0008 0.0003 0.0006 0.0001 0.0007 0.0001 0.0002 0.0003 0.0003 0.0003 0.0002 0.0003 0.0003 0
Nucleus 0.0387 0.0299 0.0305 0.0221 0.0168 0.0292 0.0324 0.0095 0.0142 0.0085 0.006 0.0309 0.0197 0.0131 0.0197 0.0213 0.0124 0.0226
Peroxisomes 0.0095 0.0006 0.001 0.0015 0.0044 0.0005 0.0038 0.0004 0.003 0.0006 0.0641 0.0044 0.0021 0.0002 0.0029 0.0003 0.0272 0.0001
Punctate Nuclear 0.0088 0.005 0.0117 0.0103 0.0151 0.0073 0.0078 0.0015 0.0054 0.0009 0.0035 0.0023 0.0035 0.0015 0.003 0.0029 0.003 0.0013
Vacuole 0.0057 0.0074 0.0059 0.0057 0.0045 0.0039 0.0036 0.0026 0.0037 0.0031 0.004 0.0023 0.0086 0.0056 0.0033 0.0068 0.005 0.0019
Vacuole Periphery 0.0014 0.0008 0.0013 0.0011 0.0008 0.0007 0.0011 0.0002 0.0009 0.0006 0.001 0.0011 0.0021 0.0006 0.0011 0.0012 0.001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.2537 32.6869 289.6346 999.0893 367.4135 19.3473 25.7593 63.6387 689.2058 550.8899
Translational Efficiency 0.9827 1.2488 1.9546 1.4686 2.3735 1.1958 2.1848 1.6336 2.3117 2.5243

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
Localization
Cell Percentages ER (32%), cytoplasm (28%), mixed (31%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00031)
Subcompartmental Group N/A

Pir1

Pir1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pir1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available