Standard name
Human Ortholog
Description Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.48 0.48 0.69 0.75 0.78 0.78 0.74 – 0.84 0.88 0.82 0.41 0.42 0.55 0.5 0.44 0.44 0 0.08 0 0 0 0
Endoplasmic Reticulum 0 0.08 0.11 0.09 0.08 0.12 0.08 0.11 – 0.06 0 0.11 0.18 0.22 0.14 0 0 0 0.17 0.13 0.27 0.21 0.1 0.07
Endosome 0.11 0 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.73 0.51 0.48 0.3 0.21 0.11 0.18 0.14 – 0.1 0.1 0.09 0.46 0.44 0.31 0.57 0.62 0.63 0.74 0.68 0.59 0.7 0.77 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 3 6 6 – 6 0 2 0 0 0 0 1 0 0 1 0 0 0 0
Bud 0 0 0 3 1 2 6 11 – 12 15 9 0 0 0 1 0 0 0 4 1 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 1 4 3 6 – 8 7 10 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 24 63 128 207 277 326 465 388 – 534 350 400 63 63 89 102 187 98 4 14 9 2 9 10
Endoplasmic Reticulum 3 10 28 26 29 49 45 55 – 41 16 54 27 33 23 6 10 3 21 23 84 29 32 22
Endosome 9 0 14 15 7 5 15 9 – 9 4 3 1 1 2 5 15 3 2 4 15 2 16 12
Golgi 0 1 0 0 0 0 0 0 – 0 0 0 1 1 0 1 0 2 0 0 1 1 1 1
Mitochondria 0 1 4 3 1 5 8 7 – 9 9 6 1 1 0 7 12 1 0 2 5 0 3 2
Nucleus 3 0 3 1 0 1 2 8 – 1 0 4 3 3 5 14 21 12 0 1 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 2 4 – 4 1 4 0 0 0 0 0 0 1 2 5 2 5 4
Nucleolus 0 0 0 0 0 2 0 1 – 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 61 67 127 90 79 45 106 74 – 65 38 46 70 66 51 116 266 141 92 116 185 100 255 244
Unique Cell Count 83 131 266 299 371 418 596 521 638 400 490 152 151 162 202 429 224 126 172 313 143 332 307
Labelled Cell Count 101 142 304 346 396 442 658 569 691 441 538 166 168 172 253 512 260 126 172 313 143 332 307


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.8 7.5 7.8 8.7 7.4 7.3 7.4 – 7.4 7.4 7.5 9.2 9.2 9.2 7.6 8.6 11.5 9.6 9.5 9.8
Std Deviation (1e-4) 1.3 1.7 1.6 1.8 1.8 1.6 1.6 1.5 – 1.6 1.7 1.8 1.7 1.7 1.7 2.1 2.4 3.3 1.8 1.7 1.9
Intensity Change (Log2) – – – 0.06 0.22 -0.02 -0.04 -0.02 – -0.03 -0.02 0.0 0.29 0.29 0.29 0.03 0.19 0.62 0.35 0.33 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 3.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 5.1 6.9 8.1 8.8 7.4 – 11.0 11.1 9.6 -1.3 -1.3 1.4 0.5 -1.2 -1.0
Endoplasmic Reticulum -0.7 -1.2 0.5 -1.4 0 – -2.1 -3.3 0.2 2.1 3.1 1.1 -3.1 -4.6 -4.2
Endosome -0.1 -2.4 -3.2 -2.1 -2.8 – -3.4 -3.3 -4.1 -2.4 -2.4 -2.1 -1.5 -1.1 -2.4
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0.7 0 0 0 0 0 1.1 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 3.3 2.6 2.7
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -4.3 -7.0 -10.9 -9.1 -10.2 – -12.6 -11.2 -12.0 -0.3 -0.8 -3.3 2.1 3.7 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6103 7.6142 7.7255 6.7073 7.0938 6.8731 3.6637 5.6759 7.0695 6.9155 5.2039 5.4477 3.2792 6.18 6.1496 6.3486 4.3191 4.7784
Actin 0.0222 0.0001 0.013 0.075 0.0018 0.0011 0.0308 0 0.0029 0.0001 0.0247 0.0002 0.0232 0.0001 0.0005 0 0.0221 0.0007
Bud 0.0006 0.0003 0.0046 0.0011 0.0003 0.0003 0.0011 0.0004 0.0005 0.0008 0.0016 0.0007 0.0005 0.0002 0.0003 0.0005 0.0009 0.0003
Bud Neck 0.0021 0.0009 0.0011 0.0008 0.0004 0.001 0.0006 0.0012 0.0008 0.0004 0.0027 0.0008 0.0006 0.0004 0.0007 0.0003 0.0008 0.0014
Bud Periphery 0.0015 0.0006 0.0031 0.002 0.0009 0.0008 0.0009 0.0008 0.0008 0.0021 0.0027 0.0025 0.0009 0.0006 0.0008 0.0015 0.0013 0.0011
Bud Site 0.0022 0.0016 0.0032 0.0034 0.0005 0.0004 0.0577 0.0049 0.0012 0.0006 0.0058 0.0007 0.0028 0.0007 0.0011 0.0002 0.0119 0.0005
Cell Periphery 0.0021 0.0031 0.002 0.0013 0.0012 0.0014 0.0017 0.0065 0.0049 0.002 0.0034 0.002 0.0031 0.0017 0.0023 0.001 0.0005 0.0017
Cytoplasm 0.0324 0.0908 0.075 0.0209 0.0309 0.0351 0.0373 0.0676 0.0658 0.0175 0.0111 0.0434 0.0429 0.1067 0.0657 0.0169 0.0577 0.0372
Cytoplasmic Foci 0.0202 0.0037 0.0049 0.0081 0.0118 0.0043 0.0085 0.0251 0.015 0.0008 0.017 0.0023 0.0111 0.0023 0.0083 0.0009 0.0132 0.0026
Eisosomes 0.0001 0 0.0002 0.0002 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0004 0
Endoplasmic Reticulum 0.0984 0.1408 0.1821 0.1124 0.0774 0.0744 0.1054 0.1992 0.2857 0.2353 0.0867 0.1137 0.0569 0.1545 0.276 0.1086 0.0453 0.0763
Endosome 0.123 0.0551 0.0834 0.1121 0.1303 0.0744 0.1391 0.0797 0.13 0.0619 0.2027 0.1097 0.1432 0.0419 0.1053 0.0701 0.1175 0.0802
Golgi 0.0122 0.0007 0.0029 0.0079 0.0065 0.0017 0.0026 0.0013 0.0057 0.0006 0.031 0.002 0.008 0.0004 0.0089 0.0007 0.0075 0.0018
Lipid Particles 0.0114 0.0018 0.0042 0.0027 0.0323 0.0027 0.0102 0.0161 0.0185 0.0017 0.0327 0.002 0.0084 0.0038 0.0122 0.0007 0.0142 0.0051
Mitochondria 0.0071 0.0008 0.0042 0.0084 0.0033 0.0012 0.0009 0.0011 0.0036 0.0008 0.0664 0.005 0.0059 0.0004 0.0048 0.0009 0.0082 0.0015
None 0.0032 0.0009 0.0018 0.0006 0.0049 0.0004 0.0009 0.0003 0.0005 0.0001 0.0002 0.0005 0.0053 0.0204 0.0004 0.0001 0.0044 0.0019
Nuclear Periphery 0.0718 0.0519 0.0613 0.0573 0.0688 0.0682 0.0442 0.072 0.0706 0.054 0.0285 0.067 0.0863 0.0818 0.0657 0.0642 0.0452 0.0858
Nucleolus 0.0015 0.0056 0.0006 0.0004 0.0004 0.0007 0.0009 0.0131 0.0003 0.0002 0.0023 0.0004 0.002 0.0005 0.0004 0.0001 0.0054 0.0008
Nucleus 0.0217 0.0109 0.0094 0.0049 0.0109 0.0183 0.0075 0.0126 0.0112 0.0043 0.0036 0.0065 0.021 0.0092 0.0055 0.0043 0.0272 0.0092
Peroxisomes 0.0042 0.0001 0.0013 0.0095 0.0057 0.0025 0.0011 0.0014 0.023 0 0.0055 0 0.0046 0 0.0006 0 0.0051 0.0009
Punctate Nuclear 0.0113 0.0003 0.0012 0.0009 0.0024 0.0014 0.0007 0.0004 0.0009 0.0001 0.0007 0.0002 0.0055 0.0003 0.0005 0 0.018 0.0043
Vacuole 0.4851 0.5744 0.4831 0.5108 0.5051 0.595 0.4673 0.4535 0.3215 0.5281 0.365 0.5269 0.4749 0.5046 0.3931 0.6182 0.4592 0.5332
Vacuole Periphery 0.0656 0.0557 0.0573 0.0593 0.104 0.1148 0.0804 0.0428 0.0367 0.0886 0.1056 0.1136 0.0928 0.0695 0.0469 0.1109 0.1339 0.1536

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.167 26.8037 12.1989 22.3763 34.9878 38.358 25.7461 18.1695 17.5047 49.2255
Translational Efficiency 0.9123 0.7934 0.8936 0.5837 0.8116 1.4613 0.8894 0.6833 0.8325 0.8519

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol
Localization
Cell Percentages ER (42%), vacuole (10%), mixed (44%)
Cell Cycle Regulation Yes
vacuole - MA (0.00048)
Subcompartmental Group N/A

Mcd4

Mcd4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcd4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available