Standard name
Human Ortholog
Description Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.08 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.97 1.0 0.99 0.99 0.98 0.96 0.94 0.87 – 0.78 0.84 0.99 1.0 0.99 0.97 0.94 0.99 0.93 0.97 0.89 0.81 0.8 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.09 0.11 0.05 0.25 – 0.36 0.22 0 0 0 0 0 0 0 0 0 0.08 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 1 0 – 0 1 0 0 0 1 0 0 0 0 0 2 3 9
Bud 1 1 0 1 1 1 5 7 7 – 9 12 2 2 1 0 1 0 0 0 0 0 6 5
Bud Neck 0 0 3 5 1 2 4 12 3 – 3 6 0 0 0 0 0 0 0 1 1 2 4 3
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 5 4 4 4 6 5 6 – 4 6 3 5 7 39 19 15 0 0 1 2 4 3
Cytoplasm 123 153 270 336 388 408 459 490 356 – 288 358 275 512 650 423 210 160 119 160 235 158 325 308
Endoplasmic Reticulum 2 1 0 0 0 0 0 0 1 – 0 0 2 0 2 14 8 6 2 0 1 2 6 5
Endosome 0 0 1 0 0 0 0 1 0 – 2 2 0 2 0 1 0 0 1 0 2 1 1 2
Golgi 0 0 0 1 0 0 0 0 0 – 0 0 1 0 0 1 4 0 0 0 0 1 0 2
Mitochondria 7 1 0 3 3 36 52 25 104 – 131 95 0 0 2 6 2 1 1 1 8 15 32 19
Nucleus 0 1 1 4 1 0 0 3 1 – 2 0 0 1 1 1 2 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 1 2 – 2 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 2 0 2 5 0 – 1 3 0 0 0 0 0 0 0 0 1 0 1 3
Vac/Vac Membrane 0 0 0 0 0 2 1 6 0 – 3 6 0 0 0 6 3 2 1 1 5 5 13 18
Unique Cell Count 128 157 271 340 392 417 479 519 408 367 426 278 514 654 438 224 162 128 166 264 196 407 392
Labelled Cell Count 138 164 280 355 400 453 531 556 480 445 489 283 523 664 492 250 185 128 166 264 196 407 392


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.7 5.3 4.9 5.6 4.3 4.5 4.8 4.0 – 3.8 4.3 4.9 5.0 5.1 7.4 7.8 7.1 4.5 4.4 5.0
Std Deviation (1e-4) 0.5 0.6 1.1 1.4 1.6 1.3 1.3 1.2 1.2 – 1.1 0.7 1.1 0.9 1.0 1.1 1.2 1.1 1.2 0.9 1.5
Intensity Change (Log2) – – – -0.1 0.1 -0.29 -0.23 -0.12 -0.4 – -0.47 -0.3 -0.1 -0.08 -0.04 0.5 0.58 0.43 -0.22 -0.27 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8333 0.3152 -0.1936 0.5053 1.6046 -0.1356 -0.8404 -0.5971 -0.74 -0.58 -0.9128 -0.9404 -0.0164 0.1425 -0.2765 -0.244 -0.3476 0.0141
Actin 0.0575 0.0006 0.0243 0.0204 0.0052 0.0002 0.0315 0.0006 0.011 0.0256 0.0022 0.0014 0.024 0.0047 0.0115 0.0001 0.0001 0.0023
Bud 0.0006 0.0008 0.0048 0.0004 0.0016 0 0.0014 0.0016 0.001 0.0031 0.0005 0.0002 0.0008 0.0001 0.0014 0.0001 0 0.0002
Bud Neck 0.0026 0.0002 0.0032 0.0005 0.0005 0.0002 0.0058 0.0002 0.0004 0.0049 0.0031 0.0006 0.0011 0.0001 0.0015 0.0001 0 0.0003
Bud Periphery 0.0012 0.0009 0.0043 0.0003 0.0013 0 0.0013 0.0012 0.0017 0.0053 0.0005 0.0004 0.001 0.0001 0.0012 0.0001 0 0.0004
Bud Site 0.0041 0.0175 0.0123 0.001 0.0006 0 0.02 0.006 0.0123 0.0118 0.0257 0.0008 0.0089 0.0009 0.0071 0.0001 0 0.0002
Cell Periphery 0.0008 0.0012 0.0008 0.0001 0.0002 0.0001 0.0006 0.0004 0.0004 0.0007 0.0002 0.0001 0.0008 0.0003 0.0003 0.0001 0 0.0001
Cytoplasm 0.3158 0.4787 0.32 0.3153 0.4639 0.4551 0.3826 0.6591 0.4994 0.5708 0.4979 0.6448 0.398 0.674 0.5551 0.6278 0.3417 0.7696
Cytoplasmic Foci 0.0448 0.0048 0.0341 0.0648 0.0041 0.0122 0.0666 0.0144 0.0303 0.0133 0.0416 0.009 0.0206 0.0052 0.0146 0.0073 0.0023 0.0055
Eisosomes 0.0025 0.0005 0.0005 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0005 0.0002 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0055 0.002 0.0033 0.0032 0.0047 0.0026 0.0051 0.003 0.0039 0.0042 0.0015 0.0028 0.0081 0.006 0.0039 0.0022 0.0012 0.0032
Endosome 0.0162 0.0016 0.0138 0.041 0.0026 0.0006 0.0427 0.0057 0.0142 0.0231 0.0094 0.0107 0.0499 0.0035 0.0065 0.0056 0.0006 0.015
Golgi 0.0061 0.0001 0.0106 0.037 0.0002 0.0001 0.0159 0.0005 0.0042 0.0116 0.0136 0.0021 0.0153 0.0031 0.0059 0.0001 0 0.0033
Lipid Particles 0.0114 0.0002 0.0152 0.0148 0.0001 0.0005 0.0186 0.0005 0.0036 0.0149 0.0356 0.0037 0.0243 0.0012 0.0016 0.0001 0.0001 0.0022
Mitochondria 0.0047 0.0002 0.0167 0.0058 0.0027 0.0001 0.0064 0.0003 0.0164 0.0264 0.0012 0.0114 0.0107 0.0012 0.0017 0.0001 0.0001 0.0295
None 0.4947 0.4869 0.4886 0.4375 0.3122 0.5271 0.3422 0.2995 0.382 0.261 0.3403 0.3063 0.39 0.296 0.3812 0.3514 0.6527 0.1625
Nuclear Periphery 0.0048 0.0004 0.002 0.0007 0.0754 0.0002 0.0095 0.0007 0.0017 0.0049 0.0002 0.0006 0.0105 0.0009 0.0009 0.0007 0.0003 0.0014
Nucleolus 0.0013 0.0002 0.0075 0.0001 0.0004 0 0.0009 0.0001 0.0004 0.0024 0.0004 0.0005 0.0049 0.0001 0.0002 0 0 0.0002
Nucleus 0.0018 0.0008 0.0047 0.0005 0.1172 0.0003 0.0037 0.0011 0.0018 0.0023 0.0004 0.0009 0.0041 0.0005 0.0007 0.0008 0.0002 0.0006
Peroxisomes 0.0132 0.0001 0.0224 0.0527 0.0002 0.0001 0.0321 0.0003 0.0079 0.0019 0.0236 0.0007 0.0044 0.0001 0.0017 0 0 0.0007
Punctate Nuclear 0.0079 0.0006 0.0071 0.001 0.0019 0.0003 0.0021 0.0007 0.0016 0.001 0.0008 0.0006 0.0125 0.0003 0.0011 0.0003 0.0001 0.0009
Vacuole 0.0019 0.0015 0.0033 0.0023 0.0029 0.0003 0.0081 0.0034 0.0041 0.0037 0.001 0.0017 0.0067 0.0015 0.0015 0.0025 0.0004 0.0012
Vacuole Periphery 0.0005 0.0001 0.0006 0.0005 0.0022 0 0.0028 0.0004 0.0017 0.0071 0.0002 0.0005 0.0029 0.0002 0.0003 0.0004 0.0001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5951 4.9886 10.7687 10.0498 6.5775 6.0844 10.0879 10.5224 13.2469 11.0582
Translational Efficiency 0.5797 0.644 0.419 0.5402 0.7118 0.8648 0.4723 0.4652 0.5514 0.4657

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Kkq8

Kkq8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kkq8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available